LIVIVO - Search results -

Search results

Result 1 - 10 of total 11

Search options

Article ; Online: Rapid detection of KPC-producing Enterobacterales by using a modified Carba NP test with imipenem/relebactam.

Oviaño, Marina / Ramírez, Cecilia de la Luna / González-Bardanca, Mónica / Candela, Ana / Bou, Germán

Enfermedades infecciosas y microbiologia clinica (English ed.)

2022 Volume 40, Issue 10, Page(s) 568–571

Abstract: Introduction: Here, we propose a novel modified Carba NP test for detecting KPC-producing Enterobacterales using imipenem/relebactam.: Material and methods: The test performance was evaluated in a random selection of 160 previously molecularly ... ...

Abstract	Introduction: Here, we propose a novel modified Carba NP test for detecting KPC-producing Enterobacterales using imipenem/relebactam. Material and methods: The test performance was evaluated in a random selection of 160 previously molecularly characterized clinical isolates carrying the 110 bla Results: All class A producing Enterobacterales (111/111) were detected using imipenem/relebactam as no visual appreciation of color change was perceived due to a nule hydrolysis of imipenem in the antibiotic solution. Overall, the assay showed 100% sensitivity (111/111) and specificity (69/69) for detecting class A KPC-producing Enterobacterales. Discussion: The biochemical assay provides very reliable results for detecting KPC-producing Enterobacterales, with a turnaround time of less than 1 hour, minimum handling and no specialized equipment required.
MeSH term(s)	Gammaproteobacteria ; Imipenem/pharmacology ; Anti-Bacterial Agents/pharmacology ; Azabicyclo Compounds
Chemical Substances	relebactam (Y1MYA2UHFL) ; Imipenem (71OTZ9ZE0A) ; Anti-Bacterial Agents ; Azabicyclo Compounds
Language	English
Publishing date	2022-11-01
Publishing country	Spain
Document type	Journal Article
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimce.2021.03.010
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Rapid detection of KPC-producing Enterobacterales by using a modified Carba NP test with imipenem/relebactam.

Oviaño, Marina / Ramírez, Cecilia de la Luna / González-Bardanca, Mónica / Candela, Ana / Bou, Germán

Enfermedades infecciosas y microbiologia clinica (English ed.)

2021

Abstract	Introduction: Here, we propose a novel modified Carba NP test for detecting KPC-producing Enterobacterales using imipenem/relebactam. Material and methods: The test performance was evaluated in a random selection of 160 previously molecularly characterized clinical isolates carrying the 110 bla Results: All class A producing Enterobacterales (111/111) were detected using imipenem/relebactam as no visual appreciation of color change was perceived due to a nule hydrolysis of imipenem in the antibiotic solution. Overall, the assay showed 100% sensitivity (111/111) and specificity (69/69) for detecting class A KPC-producing Enterobacterales. Discussion: The biochemical assay provides very reliable results for detecting KPC-producing Enterobacterales, with a turnaround time of less than 1 hour, minimum handling and no specialized equipment required.
Language	Spanish
Publishing date	2021-04-15
Publishing country	Spain
Document type	Journal Article
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimc.2021.03.013
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Strategies to Combat Multidrug-Resistant and Persistent Infectious Diseases.

Pacios, Olga / Blasco, Lucia / Bleriot, Inès / Fernandez-Garcia, Laura / González Bardanca, Mónica / Ambroa, Antón / López, María / Bou, German / Tomás, Maria

Antibiotics (Basel, Switzerland)

2020 Volume 9, Issue 2

Abstract: Antibiotic failure is one of the most worrying health problems worldwide. We are currently facing an international crisis with several problematic facets: new antibiotics are no longer being discovered, resistance mechanisms are occurring in almost all ... ...

Abstract	Antibiotic failure is one of the most worrying health problems worldwide. We are currently facing an international crisis with several problematic facets: new antibiotics are no longer being discovered, resistance mechanisms are occurring in almost all clinical isolates of bacteria, and recurrent infections caused by persistent bacteria are hampering the successful treatment of infections. In this context, new anti-infectious strategies against multidrug-resistant (MDR) and persistent bacteria, as well as the rescue of Food and Drug Administration (FDA)-approved compounds (drug repurposing), are being explored. Among the highlighted new anti-infectious strategies, in this review, we focus on antimicrobial peptides, anti-virulence compounds, phage therapy, and new molecules. As drugs that are being repurposed, we highlight anti-inflammatory compounds, anti-psychotics, anti-helminthics, anti-cancerous drugs, and statins.
Language	English
Publishing date	2020-02-06
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2681345-2
ISSN	2079-6382
ISSN	2079-6382
DOI	10.3390/antibiotics9020065
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Viral Related Tools against SARS-CoV-2.

Fernandez-Garcia, Laura / Pacios, Olga / González-Bardanca, Mónica / Blasco, Lucia / Bleriot, Inés / Ambroa, Antón / López, María / Bou, German / Tomás, Maria

Viruses

2020 Volume 12, Issue 10

Abstract: At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2. As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to ... ...

Abstract	At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2. As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to fight against it. Here we summarize the recent research on viruses employed as therapy and diagnostic of COVID-19: (i) viral-vector vaccines both in clinical trials and pre-clinical phases; (ii) the use of bacteriophages to find antibodies specific to this virus and some studies of how to use the bacteriophages themselves as a treatment against viral diseases; and finally, (iii) the use of CRISPR-Cas technology both to obtain a fast precise diagnose of the patient and also the possible use of this technology as a cure.
MeSH term(s)	Antibodies, Viral/immunology ; Antibodies, Viral/therapeutic use ; Antiviral Agents/therapeutic use ; Bacteriophages ; Betacoronavirus/isolation & purification ; COVID-19 ; CRISPR-Cas Systems ; Coronavirus Infections/therapy ; Coronavirus Infections/virology ; Humans ; Pandemics ; Phage Therapy ; Pneumonia, Viral/therapy ; Pneumonia, Viral/virology ; SARS-CoV-2 ; Viral Vaccines/immunology
Chemical Substances	Antibodies, Viral ; Antiviral Agents ; Viral Vaccines
Keywords	covid19
Language	English
Publishing date	2020-10-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v12101172
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Viral Related Tools against SARS-CoV-2

Fernandez-Garcia, Laura / Pacios, Olga / González-Bardanca, Mónica / Blasco, Lucia / Bleriot, Inés / Ambroa, Antón / López, María / Bou, German / Tomás, Maria

Viruses. 2020 Oct. 16, v. 12, no. 10

2020

Abstract	At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2. As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to fight against it. Here we summarize the recent research on viruses employed as therapy and diagnostic of COVID-19: (i) viral-vector vaccines both in clinical trials and pre-clinical phases; (ii) the use of bacteriophages to find antibodies specific to this virus and some studies of how to use the bacteriophages themselves as a treatment against viral diseases; and finally, (iii) the use of CRISPR-Cas technology both to obtain a fast precise diagnose of the patient and also the possible use of this technology as a cure.
Keywords	COVID-19 infection ; CRISPR-Cas systems ; Severe acute respiratory syndrome coronavirus 2 ; antibodies ; bacteriophages ; clinical trials ; patients ; therapeutics ; vaccines
Language	English
Dates of publication	2020-1016
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v12101172
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: CRISPR-Cas, a Revolution in the Treatment and Study of ESKAPE Infections: Pre-Clinical Studies.

González de Aledo, Manuel / González-Bardanca, Mónica / Blasco, Lucía / Pacios, Olga / Bleriot, Inés / Fernández-García, Laura / Fernández-Quejo, Melisa / López, María / Bou, Germán / Tomás, María

Antibiotics (Basel, Switzerland)

2021 Volume 10, Issue 7

Abstract: One of the biggest threats we face globally is the emergence of antimicrobial-resistant (AMR) bacteria, which runs in parallel with the lack in the development of new antimicrobials. Among these AMR bacteria pathogens belonging to the ESKAPE group can be ...

Abstract	One of the biggest threats we face globally is the emergence of antimicrobial-resistant (AMR) bacteria, which runs in parallel with the lack in the development of new antimicrobials. Among these AMR bacteria pathogens belonging to the ESKAPE group can be highlighted (
Language	English
Publishing date	2021-06-22
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2681345-2
ISSN	2079-6382
ISSN	2079-6382
DOI	10.3390/antibiotics10070756
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Enhanced Antibacterial Activity of Repurposed Mitomycin C and Imipenem in Combination with the Lytic Phage vB_KpnM-VAC13 against Clinical Isolates of Klebsiella pneumoniae.

Pacios, Olga / Fernández-García, Laura / Bleriot, Ines / Blasco, Lucía / González-Bardanca, Mónica / López, María / Fernández-Cuenca, Felipe / Oteo, Jesús / Pascual, Álvaro / Martínez-Martínez, Luis / Domingo-Calap, Pilar / Bou, Germán / Tomás, María

Antimicrobial agents and chemotherapy

2021 Volume 65, Issue 9, Page(s) e0090021

Abstract: Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that employs different strategies (resistance and persistence) to counteract antibiotic treatments. This study aimed to search for new means of combatting imipenem-resistant and persister ... ...

Abstract	Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that employs different strategies (resistance and persistence) to counteract antibiotic treatments. This study aimed to search for new means of combatting imipenem-resistant and persister strains of K. pneumoniae by repurposing the anticancer drug mitomycin C as an antimicrobial agent and by combining the drug and the conventional antibiotic imipenem with the lytic phage vB_KpnM-VAC13. Several clinical K. pneumoniae isolates were characterized, and an imipenem-resistant isolate (harboring OXA-245 β-lactamase) and a persister isolate were selected for study. The mitomycin C and imipenem MICs for both isolates were determined by the broth microdilution method. Time-kill curve data were obtained by optical density at 600 nm (OD
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacteriophages ; Humans ; Imipenem/pharmacology ; Klebsiella Infections ; Klebsiella pneumoniae ; Microbial Sensitivity Tests ; Mitomycin/pharmacology ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; Mitomycin (50SG953SK6) ; Imipenem (71OTZ9ZE0A) ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2021-08-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.00900-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 809: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Einzelsignatur: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Activity of imipenem/relebactam against a Spanish nationwide collection of carbapenemase-producing Enterobacterales.

Vázquez-Ucha, Juan Carlos / Seoane-Estévez, Alejandro / Rodiño-Janeiro, Bruno Kotska / González-Bardanca, Mónica / Conde-Pérez, Kelly / Martínez-Guitián, Marta / Alvarez-Fraga, Laura / Arca-Suárez, Jorge / Lasarte-Monterrubio, Cristina / Gut, Marta / Gut, Ivo / Álvarez-Tejado, Miguel / Oviaño, Marina / Beceiro, Alejandro / Bou, Germán

The Journal of antimicrobial chemotherapy

2021 Volume 76, Issue 6, Page(s) 1498–1510

Abstract: Background: Imipenem/relebactam is a novel carbapenem/β-lactamase inhibitor combination, developed to act against carbapenemase-producing Enterobacterales (CPE).: Objectives: To assess the in vitro activity of imipenem/relebactam against a Spanish ... ...

Abstract	Background: Imipenem/relebactam is a novel carbapenem/β-lactamase inhibitor combination, developed to act against carbapenemase-producing Enterobacterales (CPE). Objectives: To assess the in vitro activity of imipenem/relebactam against a Spanish nationwide collection of CPE by testing the susceptibility of these isolates to 16 widely used antimicrobials and to determine the underlying β-lactam resistance mechanisms involved and the molecular epidemiology of carbapenemases in Spain. Materials and methods: Clinical CPE isolates (n = 401) collected for 2 months from 24 hospitals in Spain were tested. MIC50, MIC90 and susceptibility/resistance rates were interpreted in accordance with the EUCAST guidelines. β-Lactam resistance mechanisms and molecular epidemiology were characterized by WGS. Results: For all isolates, high rates of susceptibility to colistin (86.5%; MIC50/90 = 0.12/8 mg/L), imipenem/relebactam (85.8%; MIC50/90 = 0.5/4 mg/L) and ceftazidime/avibactam (83.8%, MIC50/90 = 1/≥256 mg/L) were observed. The subgroups of isolates producing OXA-48-like (n = 305, 75.1%) and KPC-like enzymes (n = 44, 10.8%) were highly susceptible to ceftazidime/avibactam (97.7%, MIC50/90 = 1/2 mg/L) and imipenem/relebactam (100.0%, MIC50/90 = ≤0.25/1 mg/L), respectively.The most widely disseminated high-risk clones of carbapenemase-producing Klebsiella pneumoniae across Spain were found to be ST11, ST147, ST392 and ST15 (mostly associated with OXA-48) and ST258/512 (in all cases producing KPC). Conclusions: Imipenem/relebactam, colistin and ceftazidime/avibactam were the most active antimicrobials against all CPEs. Imipenem/relebactam is a valuable addition to the antimicrobial arsenal used in the fight against CPE, particularly against KPC-producing isolates, which in all cases were susceptible to this combination.
Language	English
Publishing date	2021-02-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dkab043
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 124: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Viral Related Tools against SARS-CoV-2

Fernandez-Garcia, Laura / Pacios, Olga / González-Bardanca, Mónica Blasco / Lucia, Bleriot / Inés, Ambroa / Antón, López María Bou / German, Tomás Maria

Viruses

Abstract: At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2 As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to ... ...

Abstract	At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2 As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to fight against it Here we summarize the recent research on viruses employed as therapy and diagnostic of COVID-19: (i) viral-vector vaccines both in clinical trials and pre-clinical phases;(ii) the use of bacteriophages to find antibodies specific to this virus and some studies of how to use the bacteriophages themselves as a treatment against viral diseases;and finally, (iii) the use of CRISPR-Cas technology both to obtain a fast precise diagnose of the patient and also the possible use of this technology as a cure
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #875274
Database	COVID19

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Multiplex Real-Time PCR-short

Alcaide, Fernando / Trastoy, Rocío / Moure, Raquel / González-Bardanca, Mónica / Ambroa, Antón / López, María / Bleriot, Inés / Blasco, Lucia / Fernandez-García, Laura / Tato, Marta / Bou, German / Tomás, María

Journal of clinical microbiology

2019 Volume 57, Issue 8

Abstract: Tuberculosis (TB) remains a major health problem worldwide. Control of TB requires rapid, accurate diagnosis of active disease. However, extrapulmonary TB is very difficult to diagnose because the clinical specimens have very low bacterial loads. Several ...

Abstract	Tuberculosis (TB) remains a major health problem worldwide. Control of TB requires rapid, accurate diagnosis of active disease. However, extrapulmonary TB is very difficult to diagnose because the clinical specimens have very low bacterial loads. Several molecular methods involving direct detection of the
MeSH term(s)	ATP-Binding Cassette Transporters/genetics ; Bacterial Load/methods ; Bacterial Proteins/genetics ; Humans ; Limit of Detection ; Lung/microbiology ; Multiplex Polymerase Chain Reaction/methods ; Multiplex Polymerase Chain Reaction/standards ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/growth & development ; Oligonucleotides/genetics ; Pleural Effusion/microbiology ; Sensitivity and Specificity ; Tuberculosis/microbiology
Chemical Substances	ATP-Binding Cassette Transporters ; Bacterial Proteins ; Oligonucleotides ; PstS-1 protein, Mycobacterium tuberculosis ; locked nucleic acid
Language	English
Publishing date	2019-07-26
Publishing country	United States
Document type	Comparative Study ; Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.00733-19
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1188: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito