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  1. Article ; Online: Water-soluble polymers in agriculture: xanthan gum as eco-friendly alternative to synthetics.

    Berninger, Teresa / Dietz, Natalie / González López, Óscar

    Microbial biotechnology

    2021  Volume 14, Issue 5, Page(s) 1881–1896

    Abstract: Water-soluble polymers (WSPs) are a versatile group of chemicals used across industries for different purposes such as thickening, stabilizing, adhesion and gelation. Synthetic polymers have tailored characteristics and are chemically homogeneous, ... ...

    Abstract Water-soluble polymers (WSPs) are a versatile group of chemicals used across industries for different purposes such as thickening, stabilizing, adhesion and gelation. Synthetic polymers have tailored characteristics and are chemically homogeneous, whereas plant-derived biopolymers vary more widely in their specifications and are chemically heterogeneous. Between both sources, microbial polysaccharides are an advantageous compromise. They combine naturalness with defined material properties, precisely controlled by optimizing strain selection, fermentation operational parameters and downstream processes. The relevance of such bio-based and biodegradable materials is rising due to increasing environmental awareness of consumers and a tightening regulatory framework, causing both solid and water-soluble synthetic polymers, also termed 'microplastics', to have come under scrutiny. Xanthan gum is the most important microbial polysaccharide in terms of production volume and diversity of applications, and available as different grades with specific properties. In this review, we will focus on the applicability of xanthan gum in agriculture (drift control, encapsulation and soil improvement), considering its potential to replace traditionally used synthetic WSPs. As a spray adjuvant, xanthan gum prevents the formation of driftable fine droplets and shows particular resistance to mechanical shear. Xanthan gum as a component in encapsulated formulations modifies release properties or provides additional protection to encapsulated agents. In geotechnical engineering, soil amended with xanthan gum has proven to increase water retention, reduce water evaporation, percolation and soil erosion - topics of high relevance in the agriculture of the 21st century. Finally, hands-on formulation tips are provided to facilitate exploiting the full potential of xanthan gum in diverse agricultural applications and thus providing sustainable solutions.
    MeSH term(s) Agriculture ; Plastics ; Polymers ; Polysaccharides, Bacterial ; Water
    Chemical Substances Plastics ; Polymers ; Polysaccharides, Bacterial ; Water (059QF0KO0R) ; xanthan gum (TTV12P4NEE)
    Language English
    Publishing date 2021-07-01
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2406063-X
    ISSN 1751-7915 ; 1751-7915
    ISSN (online) 1751-7915
    ISSN 1751-7915
    DOI 10.1111/1751-7915.13867
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  2. Article ; Online: Obesity related pathophysiological aspects favouring Gastroesophageal Reflux Disease.

    García Ruiz de Gordejuela, Amador / Ibarzabal Olano, Ainitze / Petrola Chacón, Carlos Gustavo / González López, Óscar

    Cirugia espanola

    2023  Volume 101 Suppl 4, Page(s) S3–S7

    Abstract: Gastroesophageal reflux disease (GERD) is a complex entity and one of the most frequents in general population. Association of GERD with obesity is clearly defined from an epidemiological point of view, where up to 40% of patients with overweight and ... ...

    Abstract Gastroesophageal reflux disease (GERD) is a complex entity and one of the most frequents in general population. Association of GERD with obesity is clearly defined from an epidemiological point of view, where up to 40% of patients with overweight and obesity present reflux. Most of the risk factors associated to GERD are shared and especially frequent in patients with obesity. The pathophysiology that explain this correlation is complex and multifactorial, and includes both aspects related to physiology of motility, and anatomic changes. The malfunction of the lower esophageal sphincter, the greater transdiaphragmatic pressure gradient, pathological accumulation of fat and the high rate of hiatal hernia that comes with overweight will be the main factors that explain this correlation. Other factor like pathological eating habits or obesity related diseases have also an important role. In summary, it is the sum of the factors more than just one of them what would explain the pathophysiology of GERD in obese population.
    MeSH term(s) Humans ; Overweight/complications ; Gastroesophageal Reflux/complications ; Esophageal Sphincter, Lower ; Hernia, Hiatal/complications ; Obesity/complications ; Obesity/epidemiology
    Language English
    Publishing date 2023-11-16
    Publishing country Spain
    Document type Journal Article
    ISSN 2173-5077
    ISSN (online) 2173-5077
    DOI 10.1016/j.cireng.2022.12.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Nonlytic Quasi-Enveloped Hepatovirus Release Is Facilitated by pX Protein Interaction with the E3 Ubiquitin Ligase ITCH.

    Shirasaki, Takayoshi / González-López, Olga / McKnight, Kevin L / Xie, Ling / Shiota, Tomoyuki / Chen, Xian / Feng, Hui / Lemon, Stanley M

    Journal of virology

    2022  Volume 96, Issue 21, Page(s) e0119522

    Abstract: Hepatoviruses are atypical hepatotropic picornaviruses that are released from infected cells without lysis in small membranous vesicles. These exosome-like, quasi-enveloped virions (eHAV) are infectious and the only form of hepatitis A virus (HAV) found ... ...

    Abstract Hepatoviruses are atypical hepatotropic picornaviruses that are released from infected cells without lysis in small membranous vesicles. These exosome-like, quasi-enveloped virions (eHAV) are infectious and the only form of hepatitis A virus (HAV) found circulating in blood during acute infection. eHAV is released through multivesicular endosomes in a process dependent on endosomal sorting complexes required for transport (ESCRT). Capsid protein interactions with the ESCRT-associated Bro1 domain proteins, ALG-2-interacting protein X (ALIX) and His domain-containing protein tyrosine phosphatase (HD-PTP), which are both recruited to the pX domain of 1D (VP1pX), are critical for this process. Previous proteomics studies suggest pX also binds the HECT domain, NEDD4 family E3 ubiquitin ligase, ITCH. Here, we confirm this interaction and show ITCH binds directly to the carboxy-terminal half of pX from both human and bat hepatoviruses independently of ALIX. A small chemical compound (compound 5) designed to disrupt interactions between WW domains of NEDD4 ligases and substrate molecules blocked ITCH binding to pX and demonstrated substantial antiviral activity against HAV. CRISPR deletion or small interfering RNA (siRNA) knockdown of ITCH expression inhibited the release of a self-assembling nanocage protein fused to pX and also impaired the release of eHAV from infected cells. The release could be rescued by overexpression of wild-type ITCH, but not a catalytically inactive ITCH mutant. Despite this, we found no evidence that ITCH ubiquitylates pX or that eHAV release is strongly dependent upon Lys residues in pX. These data indicate ITCH plays an important role in the ESCRT-dependent release of quasi-enveloped hepatovirus, although the substrate molecule targeted for ubiquitylation remains to be determined.
    MeSH term(s) Humans ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; Hepatovirus/metabolism ; Capsid Proteins/genetics ; Capsid Proteins/metabolism ; Endosomal Sorting Complexes Required for Transport/metabolism ; Hepatitis A virus/physiology ; Nedd4 Ubiquitin Protein Ligases/metabolism
    Chemical Substances Ubiquitin-Protein Ligases (EC 2.3.2.27) ; Capsid Proteins ; Endosomal Sorting Complexes Required for Transport ; Nedd4 Ubiquitin Protein Ligases (EC 2.3.2.26)
    Language English
    Publishing date 2022-10-26
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/jvi.01195-22
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  4. Article: Comprehensive Analysis of Acquired Genetic Variants and Their Prognostic Impact in Systemic Mastocytosis.

    González-López, Oscar / Muñoz-González, Javier I / Orfao, Alberto / Álvarez-Twose, Iván / García-Montero, Andrés C

    Cancers

    2022  Volume 14, Issue 10

    Abstract: Systemic mastocytosis (SM) is a rare clonal haematopoietic stem cell disease in which activating KIT mutations (most commonly KIT D816V) are present in virtually every (>90%) adult patient at similar frequencies among non-advanced and advanced forms of ... ...

    Abstract Systemic mastocytosis (SM) is a rare clonal haematopoietic stem cell disease in which activating KIT mutations (most commonly KIT D816V) are present in virtually every (>90%) adult patient at similar frequencies among non-advanced and advanced forms of SM. The KIT D816V mutation is considered the most common pathogenic driver of SM. Acquisition of this mutation early during haematopoiesis may cause multilineage involvement of haematopoiesis by KIT D816V, which has been associated with higher tumour burden and additional mutations in other genes, leading to an increased rate of transformation to advanced SM. Thus, among other mutations, alterations in around 30 genes that are also frequently mutated in other myeloid neoplasms have been reported in SM cases. From these genes, 12 (i.e., ASXL1, CBL, DNMT3A, EZH2, JAK2, KRAS, NRAS, SF3B1, RUNX1, SF3B1, SRSF2, TET2) have been recurrently reported to be mutated in SM. Because of all the above, assessment of multilineage involvement of haematopoiesis by the KIT D816V mutation, in the setting of multi-mutated haematopoiesis as revealed by a limited panel of genes (i.e., ASXL1, CBL, DNMT3A, EZH2, NRAS, RUNX1 and SRSF2) and associated with a poorer patient outcome, has become of great help to identify SM patients at higher risk of disease progression and/or poor survival who could benefit from closer follow-up and eventually also early cytoreductive treatment.
    Language English
    Publishing date 2022-05-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers14102487
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  5. Article: Influence of fungicide application and vine age on Trichoderma diversity as source of biological control agents

    Carro-Huerga, G. / Mayo-Prieto, S. / Rodriguez-Gonzalez, A. / Gonzalez-Lopez, O. / Gutierrez, S. / Casquero, P. A.

    Agronomy

    2021  Volume 11, Issue 3, Art. 446, Page(s) 16 pp.

    Abstract: Fungi from the genus Trichoderma have a worldwide distribution and are commonly found in agricultural lands. Further, it has been described as a non-virulent and symbiont microorganism that can contribute to minimize the pernicious effects of pathogens. ... ...

    Institution Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS), Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, Avenida Portugal 41, 24071 León, Spain
    Abstract Fungi from the genus Trichoderma have a worldwide distribution and are commonly found in agricultural lands. Further, it has been described as a non-virulent and symbiont microorganism that can contribute to minimize the pernicious effects of pathogens. In the present work we have isolated Trichoderma spp. from bark of grapevine in different orchards in order to determine the influence of fungicide application and vine age on Trichoderma diversity in plant. An opposite correlation between the number of fungicides sprayed per campaign and the diversity of Trichoderma spp. isolates was found. Moreover, the older are grapevine plants the higher is the diversity of Trichoderma spp. isolates. The different Trichoderma strains isolated were tested against Phaeoacremonium minimum, a grapevine trunk pathogen, to evaluate their biocontrol capacity. Three Trichoderma strains shown a significant capacity to control P. minimum and were selected as candidates to be used as biological control agents. In addition, a rapid and easy method for isolating Trichoderma spp. from grapevine plants has been developed, which allowed to determine that the reduction in the amount of pesticide use, together with the preservation of old vineyards, lead to healthier agroecosystems containing higher levels of beneficial microorganisms.
    Language English
    Document type Article
    ZDB-ID 2607043-1
    ISSN 2073-4395
    ISSN 2073-4395
    Database Viticulture and Oenology Abstracts

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  6. Article ; Online: Detection of hyperfunctioning parathyroid tissue by

    Villasboas-Rosciolesi, D / García-Burillo, A / González-López, O / Caubet-Busquet, E / Castell-Conesa, J

    Revista espanola de medicina nuclear e imagen molecular

    2018  Volume 38, Issue 2, Page(s) 118–120

    Title translation Detección de tejido paratiroideo hiperfuncionante mediante PET/TC con
    MeSH term(s) Adult ; Choline/analogs & derivatives ; Humans ; Hyperparathyroidism/diagnostic imaging ; Male ; Positron Emission Tomography Computed Tomography/methods
    Chemical Substances fluorocholine (6029HGL0QP) ; Choline (N91BDP6H0X)
    Language Spanish
    Publishing date 2018-06-05
    Publishing country Spain
    Document type Case Reports ; Journal Article
    ISSN 2253-8089
    ISSN (online) 2253-8089
    DOI 10.1016/j.remn.2018.02.008
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  7. Article ; Online: Volatile Organic Compound Chamber: A Novel Technology for Microbiological Volatile Interaction Assays.

    Álvarez-García, Samuel / Mayo-Prieto, Sara / Carro-Huerga, Guzmán / Rodríguez-González, Álvaro / González-López, Óscar / Gutiérrez, Santiago / Casquero, Pedro A

    Journal of fungi (Basel, Switzerland)

    2021  Volume 7, Issue 4

    Abstract: The interest in the study of microbiological interactions mediated by volatile organic compounds (VOCs) has steadily increased in the last few years. Nevertheless, most assays still rely on the use of non-specific materials. We present a new tool, the ... ...

    Abstract The interest in the study of microbiological interactions mediated by volatile organic compounds (VOCs) has steadily increased in the last few years. Nevertheless, most assays still rely on the use of non-specific materials. We present a new tool, the volatile organic compound chamber (VOC chamber), specifically designed to perform these experiments. The novel devices were tested using four
    Language English
    Publishing date 2021-03-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof7040248
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  8. Article: Effect of Cry Toxins on

    Rodríguez-González, Álvaro / Porteous-Álvarez, Alejandra J / Guerra, Marcos / González-López, Óscar / Casquero, Pedro A / Escriche, Baltasar

    Insects

    2021  Volume 13, Issue 1

    Abstract: ... The ... ...

    Abstract The beetle
    Language English
    Publishing date 2021-12-26
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects13010027
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  9. Article: Effects of adults body size and larvae diet on the fecundity and percent fertility of eggs laid by Xylotrechus arvicola (Coleoptera: Cerambycidae) females, insect pest in Spanish vineyards

    Rodriguez-Gonzalez, A. / Pelaez, H. J. / Gonzalez-Lopez, O. / Mayo, S. / Casquero, P. A.

    Bulletin of Entomological Research

    2018  Volume 48, Issue 2, Page(s) 83–91

    Abstract: body size; eggs; fecundity; longevity; percent viability; relationship; grape; pest. ...

    Institution Research Group in Engineering and Sustainable Agriculture, Department of Engineering and Agricultural Sciences, Environment Institute Natural Resources and Biodiversity, University of Leon, Portugal Avenue 41, 24071 Leon, Spain
    Abstract body size; eggs; fecundity; longevity; percent viability; relationship; grape; pest.
    Language English
    Document type Article
    ZDB-ID 280516-9
    ISSN 1475-2670 ; 0007-4853
    ISSN (online) 1475-2670
    ISSN 0007-4853
    Database Viticulture and Oenology Abstracts

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  10. Article: Insecticidal Properties of

    Rodríguez-González, Álvaro / Álvarez-García, Samuel / González-López, Óscar / Silva, Franceli Da / Casquero, Pedro A

    Insects

    2019  Volume 10, Issue 5

    Abstract: The bean weevil, ...

    Abstract The bean weevil,
    Language English
    Publishing date 2019-05-25
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects10050151
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