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  1. Article ; Online: Purification and biochemical characterization of

    Arunachallam, Premalatha / Kumaravel, Vijayalakshmi / Gopal, Suseela Rajakumar

    Preparative biochemistry & biotechnology

    2023  Volume 54, Issue 3, Page(s) 444–453

    Abstract: The purification and biochemical characterization of the extracellular alpha amylase ... ...

    Abstract The purification and biochemical characterization of the extracellular alpha amylase from
    MeSH term(s) alpha-Amylases/chemistry ; Aspergillus ; Starch/metabolism
    Chemical Substances alpha-Amylases (EC 3.2.1.1) ; Starch (9005-25-8)
    Language English
    Publishing date 2023-07-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 1322522-4
    ISSN 1532-2297 ; 1082-6068
    ISSN (online) 1532-2297
    ISSN 1082-6068
    DOI 10.1080/10826068.2023.2235694
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 821: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Immobilization of Bacillus amyloliquefaciens MBL27 cells for enhanced antimicrobial protein production using calcium alginate beads.

    Kumaravel, Vijayalakshmi / Gopal, Suseela Rajakumar

    Biotechnology and applied biochemistry

    2010  Volume 57, Issue 3, Page(s) 97–103

    Abstract: Cell immobilization is one of the common techniques for increasing the overall cell concentration and productivity. Bacillus amyloliquefaciens MBL27 cells were immobilized in calcium alginate beads and it is a promising method for repeated AMP ( ... ...

    Abstract Cell immobilization is one of the common techniques for increasing the overall cell concentration and productivity. Bacillus amyloliquefaciens MBL27 cells were immobilized in calcium alginate beads and it is a promising method for repeated AMP (antimicrobial protein) production. The present study aimed at determining the optimal conditions for immobilization of B. amyloliquefaciens MBL27 cells in calcium alginate beads and the operational stability for enhanced production of the AMP. AMP production with free and immobilized cells was also done. In batch fermentation, maximum AMP production (7300 AU (arbitrary units)/ml against Staphylococcus aureus) was obtained with immobilized cells in shake flasks under optimized parameters such as 3% (w/v) sodium alginate, 136 mM CaCl2 with 350 alginate beads/flask of 2.7-3.0 mm diameter. In repeated cultivation, the highest activity was obtained after the second cycle of use and approx. 94% production was noted up to the fifth cycle. The immobilized cells of B. amyloliquefaciens MBL27 in alginate beads are more efficient for the production of AMP and had good stability. The potential application of AMP as a wound healant and the need for development of economical methods for improved production make whole cell immobilization an excellent alternative method for enhanced AMP production.
    MeSH term(s) Alginates/chemistry ; Anti-Infective Agents/metabolism ; Bacillus/cytology ; Bacillus/metabolism ; Bacterial Proteins/metabolism ; Cells, Immobilized/cytology ; Cells, Immobilized/metabolism ; Fermentation ; Glucuronic Acid/chemistry ; Hexuronic Acids/chemistry ; Industrial Microbiology/methods
    Chemical Substances Alginates ; Anti-Infective Agents ; Bacterial Proteins ; Hexuronic Acids ; Glucuronic Acid (8A5D83Q4RW) ; alginic acid (8C3Z4148WZ)
    Language English
    Publishing date 2010-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 883433-7
    ISSN 1470-8744 ; 0885-4513
    ISSN (online) 1470-8744
    ISSN 0885-4513
    DOI 10.1042/BA20100252
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1865: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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