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  1. Article ; Online: Site-specific analysis of N-glycans from different sheep prion strains.

    Natali Nakić / Thanh Hoa Tran / Mislav Novokmet / Olivier Andreoletti / Gordan Lauc / Giuseppe Legname

    PLoS Pathogens, Vol 17, Iss 2, p e

    2021  Volume 1009232

    Abstract: Prion diseases are a group of neurodegenerative diseases affecting a wide range of mammalian species, including humans. During the course of the disease, the abnormally folded scrapie prion protein (PrPSc) accumulates in the central nervous system where ... ...

    Abstract Prion diseases are a group of neurodegenerative diseases affecting a wide range of mammalian species, including humans. During the course of the disease, the abnormally folded scrapie prion protein (PrPSc) accumulates in the central nervous system where it causes neurodegeneration. In prion disorders, the diverse spectrum of illnesses exists because of the presence of different isoforms of PrPSc where they occupy distinct conformational states called strains. Strains are biochemically distinguished by a characteristic three-band immunoblot pattern, defined by differences in the occupancy of two glycosylation sites on the prion protein (PrP). Characterization of the exact N-glycan structures attached on either PrPC or PrPSc is lacking. Here we report the characterization and comparison of N-glycans from two different sheep prion strains. PrPSc from both strains was isolated from brain tissue and enzymatically digested with trypsin. By using liquid chromatography coupled to electrospray mass spectrometry, a site-specific analysis was performed. A total of 100 structures were detected on both glycosylation sites. The N-glycan profile was shown to be similar to the one on mouse PrP, however, with additional 40 structures reported. The results presented here show no major differences in glycan composition, suggesting that glycans may not be responsible for the differences in the two analyzed prion strains.
    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Correction

    Natali Nakić / Thanh Hoa Tran / Mislav Novokmet / Olivier Andreoletti / Gordan Lauc / Giuseppe Legname

    PLoS Pathogens, Vol 17, Iss 4, p e

    Site-specific analysis of N-glycans from different sheep prion strains.

    2021  Volume 1009511

    Abstract: This corrects the article DOI:10.1371/journal.ppat.1009232.]. ...

    Abstract [This corrects the article DOI:10.1371/journal.ppat.1009232.].
    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Comparison of 2-Aminobenzamide, Procainamide and RapiFluor-MS as Derivatizing Agents for High-Throughput HILIC-UPLC-FLR-MS N-glycan Analysis

    Toma Keser / Tamara Pavić / Gordan Lauc / Olga Gornik

    Frontiers in Chemistry, Vol

    2018  Volume 6

    Abstract: Rising awareness of the universal importance of protein N-glycosylation governs the development of further advances in N-glycan analysis. Nowadays it is well known that correct glycosylation is essential for proper protein function, which emanates from ... ...

    Abstract Rising awareness of the universal importance of protein N-glycosylation governs the development of further advances in N-glycan analysis. Nowadays it is well known that correct glycosylation is essential for proper protein function, which emanates from its important role in many physiological processes. Furthermore, glycosylation is involved in pathophysiology of multiple common complex diseases. In the vast majority of cases, N-glycosylation profiles are analyzed from enzymatically released glycans, which can be further derivatized in order to enhance the sensitivity of the analysis. Techniques wherein derivatized N-glycans are profiled using hydrophilic interaction chromatography (HILIC) with fluorescence (FLR) and mass spectrometry (MS) detection are now routinely performed in a high-throughput manner. Therefore, we aimed to examine the performance of frequently used labeling compounds −2-aminiobenzamide (2-AB) and procainamide (ProA), and the recently introduced RapiFluor-MS (RF-MS) fluorescent tag. In all experiments N-glycans were released by PNGase F, fluorescently derivatized, purified by HILIC solid phase extraction and profiled using HILIC-UPLC-FLR-MS. We assessed sensitivity, linear range, limit of quantification (LOQ), repeatability and labeling efficiency for all three labels. For this purpose, we employed in-house prepared IgG and a commercially available IgG as a model glycoprotein. All samples were analyzed in triplicates using different amounts of starting material. We also tested the performance of all three labels in a high-throughput setting on 68 different IgG samples, all in duplicates and 22 identical IgG standards. In general, ProA labeled glycans had the highest FLR sensitivity (15-fold and 4-fold higher signal intensities compared to 2-AB and RF-MS respectively) and RF-MS had the highest MS sensitivity (68-fold and 2-fold higher signal intensities compared to 2-AB and ProA, respectively). ProA and RF-MS showed comparable limits of quantification with both FLR and MS detection, whilst ...
    Keywords N-glycans ; 2-aminobenzamide ; procainamide ; RapiFluor-MS ; IgG ; HILIC ; Chemistry ; QD1-999
    Subject code 500
    Language English
    Publishing date 2018-07-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: N -Glycosylation Patterns across the Age-Related Macular Degeneration Spectrum

    Ivona Bućan / Jelena Škunca Herman / Iris Jerončić Tomić / Olga Gornik / Zoran Vatavuk / Kajo Bućan / Gordan Lauc / Ozren Polašek

    Molecules, Vol 27, Iss 1774, p

    2022  Volume 1774

    Abstract: The pathogenesis of age-related macular degeneration (AMD) remains elusive, despite numerous research studies. Therefore, we aimed to investigate the changes of plasma and IgG-specific N -glycosylation across the disease severity spectrum. We examined ... ...

    Abstract The pathogenesis of age-related macular degeneration (AMD) remains elusive, despite numerous research studies. Therefore, we aimed to investigate the changes of plasma and IgG-specific N -glycosylation across the disease severity spectrum. We examined 2835 subjects from the 10.001 Dalmatians project, originating from the isolated Croatian islands of Vis and Korčula. All subjects were classified into four groups, namely (i) bilateral AMD, (ii) unilateral AMD, (iii) early-onset drusen, and (iv) controls. We analysed plasma and IgG N -glycans measured by HPLC and their association with retinal fundus photographs. There were 106 (3.7%) detected cases of AMD; 66 of them were bilateral. In addition, 45 (0.9%) subjects were recorded as having early-onset retinal drusen. We detected several interesting differences across the analysed groups, suggesting that N -glycans can be used as a biomarker for AMD. Multivariate analysis suggested a significant decrease in the immunomodulatory bi-antennary glycan structures in unilateral AMD (adjusted odds ratio 0.43 (95% confidence interval 0.22–0.79)). We also detected a substantial increase in the pro-inflammatory tetra-antennary plasma glycans in bilateral AMD (7.90 (2.94–20.95)). Notably, some of these associations were not identified in the aggregated analysis, where all three disease stages were collapsed into a single category, suggesting the need for better-refined phenotypes and the use of disease severity stages in the analysis of more complex diseases. Age-related macular degeneration progression is characterised by the complex interplay of various mechanisms, some of which can be detected by measuring plasma and IgG N -glycans. As opposed to a simple case-control study, more advanced and refined study designs are needed to understand the pathogenesis of complex diseases.
    Keywords age-related macular degeneration ; N -glycans ; prediction ; biomarker ; discovery ; Organic chemistry ; QD241-441
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice

    Olga O. Zaytseva / Michaela Seeling / Jasminka Krištić / Gordan Lauc / Marija Pezer / Falk Nimmerjahn

    Frontiers in Cell and Developmental Biology, Vol

    2020  Volume 8

    Abstract: Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases. Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different ... ...

    Abstract Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases. Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states. Fc N-glycan composition can alter the effector functions of IgG by modulating its affinity for ligands, such as Fcγ receptors (FcγRs). However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG–FcγR interaction. To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds. We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
    Keywords Fcγ receptor ; IgG N-glycan profile ; immunoglobulin G ; N-glycosylation ; liquid chromatography–electrospray ionization–mass spectrometry ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: The Association of the Polymorphisms in the FUT8 -Related Locus with the Plasma Glycosylation in Post-Traumatic Stress Disorder

    Lucija Tudor / Gordana Nedic Erjavec / Matea Nikolac Perkovic / Marcela Konjevod / Suzana Uzun / Oliver Kozumplik / Ninoslav Mimica / Gordan Lauc / Dubravka Svob Strac / Nela Pivac

    International Journal of Molecular Sciences, Vol 24, Iss 5706, p

    2023  Volume 5706

    Abstract: The molecular underpinnings of post-traumatic stress disorder (PTSD) are still unclear due to the complex interactions of genetic, psychological, and environmental factors. Glycosylation is a common post-translational modification of proteins, and ... ...

    Abstract The molecular underpinnings of post-traumatic stress disorder (PTSD) are still unclear due to the complex interactions of genetic, psychological, and environmental factors. Glycosylation is a common post-translational modification of proteins, and different pathophysiological states, such as inflammation, autoimmune diseases, and mental disorders including PTSD, show altered N-glycome. Fucosyltransferase 8 (FUT8) is the enzyme that catalyzes the addition of core fucose on glycoproteins, and mutations in the FUT8 gene are associated with defects in glycosylation and functional abnormalities. This is the first study that investigated the associations of plasma N-glycan levels with FUT8 -related rs6573604, rs11621121, rs10483776, and rs4073416 polymorphisms and their haplotypes in 541 PTSD patients and control participants. The results demonstrated that the rs6573604 T allele was more frequent in the PTSD than in the control participants. Significant associations of plasma N-glycan levels with PTSD and FUT8 -related polymorphisms were observed. We also detected associations of rs11621121 and rs10483776 polymorphisms and their haplotypes with plasma levels of specific N-glycan species in both the control and PTSD groups. In carriers of different rs6573604 and rs4073416 genotypes and alleles, differences in plasma N-glycan levels were only found in the control group. These molecular findings suggest a possible regulatory role of FUT8 -related polymorphisms in glycosylation, the alternations of which could partially explain the development and clinical manifestation of PTSD.
    Keywords post-traumatic stress disorder ; fucosyltransferase 8 (FUT8) ; FUT8 -related polymorphisms ; glycosylation ; haplotype ; N-glycans ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 150
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Trace N-glycans including sulphated species may originate from various plasma glycoproteins and not necessarily IgG

    Gordan Lauc / Frano Vučković / Albert Bondt / Marija Pezer / Manfred Wuhrer

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 3

    Keywords Science ; Q
    Language English
    Publishing date 2018-07-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Trace N-glycans including sulphated species may originate from various plasma glycoproteins and not necessarily IgG

    Gordan Lauc / Frano Vučković / Albert Bondt / Marija Pezer / Manfred Wuhrer

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 3

    Keywords Science ; Q
    Language English
    Publishing date 2018-07-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Rapid COVID-19 Antigen Testing in Croatia

    Dragan Primorac / Vitorio Perić / Vid Matišić / Vilim Molnar / Renata Zadro / Adriana Vince / Gordan Lauc / Ozren Polašek

    Frontiers in Public Health, Vol

    Risk Perception Plays an Important Role in the Epidemic Control

    2021  Volume 9

    Abstract: Aim: To explore the clinical presentation and epidemiological history of the subjects who underwent SARS-CoV-2 antigen testing.Methods: We included 1,000 consecutive subjects who presented themselves at the diagnostic clinic in Croatia and analyzed their ...

    Abstract Aim: To explore the clinical presentation and epidemiological history of the subjects who underwent SARS-CoV-2 antigen testing.Methods: We included 1,000 consecutive subjects who presented themselves at the diagnostic clinic in Croatia and analyzed their symptoms and epidemiological history. All subjects were classified into three groups, according to their reason of arrival; symptomatic, contacts of confirmed patients, and those who were tested due to administrative reasons.Results: On average, there were 24% of positive antigen results; the positivity rate was 51% among symptomatic, 16% in contacts, and 5% of administrative patients. The commonest symptoms of the disease included febrility and anosmia. We developed a clinical score to predict SARS-CoV-2 positivity, which had an area under the curve of 79.3 [95% confidence intervals (CI) 75.8–82.8]. Contact with the isolated person [odds ratio 0.54 (95% CI 0.31–0.94)] and international travel had a protective effect [0.20 (0.09–0.43)], suggesting that risk perception and mandatory pretravel measures had a key role in the determination of the infection risk.Conclusions: A combination of clinical symptoms can have reasonable predictive power for an antigen-positive test result. Risk perception seems to have a role in the epidemic spread, probably via stricter adherence to personal preventative measures.
    Keywords SARS-CoV-2 ; risk ; perception ; antigen ; diagnostic ; pooling ; Public aspects of medicine ; RA1-1270
    Subject code 150 ; 610
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Comparative analysis of transferrin and IgG N-glycosylation in two human populations

    Irena Trbojević-Akmačić / Frano Vučković / Tea Pribić / Marija Vilaj / Urh Černigoj / Jana Vidič / Jelena Šimunović / Agnieszka Kępka / Ivana Kolčić / Lucija Klarić / Mislav Novokmet / Maja Pučić-Baković / Erdmann Rapp / Aleš Štrancar / Ozren Polašek / James F. Wilson / Gordan Lauc

    Communications Biology, Vol 6, Iss 1, Pp 1-

    2023  Volume 13

    Abstract: A large-scale comparative study in two human populations analyzes human plasma transferrin and immunoglobulin G (IgG) N-glycosylation profiles and their association with age, sex, and various biochemical and physiological traits. ...

    Abstract A large-scale comparative study in two human populations analyzes human plasma transferrin and immunoglobulin G (IgG) N-glycosylation profiles and their association with age, sex, and various biochemical and physiological traits.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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