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  1. Article ; Online: Characterization of a novel mouse platelet transfusion model.

    Gordy, Dominique / Swayne, Theresa / Berry, Gregory J / Thomas, Tiffany A / Hudson, Krystalyn E / Stone, Elizabeth F

    Vox sanguinis

    2024  

    Abstract: Background and objectives: Platelet transfusions are increasing with medical advances. Based on FDA criteria, platelet units are assessed by in vitro measures; however, it is not known how platelet processing and storage duration affect function in vivo. ...

    Abstract Background and objectives: Platelet transfusions are increasing with medical advances. Based on FDA criteria, platelet units are assessed by in vitro measures; however, it is not known how platelet processing and storage duration affect function in vivo. Our study's aim was to develop a novel platelet transfusion model stored in mouse plasma that meets FDA criteria adapted to mice, and transfused fresh and stored platelets are detectable in clots in vivo.
    Study design and methods: Platelet units stored in mouse plasma were prepared using a modified platelet-rich plasma (PRP) collection protocol. Characteristics of fresh and stored units, including pH, cell count, in vitro measures of activity, including activation and aggregation, and post-transfusion recovery (PTR), were determined. Lastly, a tail transection assay was conducted using mice transfused with fresh or stored units, and transfused platelets were identified by confocal imaging.
    Results: Platelet units had acceptable platelet and white cell counts and were negative for bacterial contamination. Fresh and 1-day stored units had acceptable pH; the platelets were activatable by thrombin and adenosine diphosphate, agreeable with thrombin, had acceptable PTR, and were present in vivo in clots of recipients after tail transection. In contrast, 2-day stored units had clinically unacceptable quality.
    Conclusion: We developed mouse platelets for transfusion analogous to human platelet units using a modified PRP collection protocol with maximum storage of 1 day for an 'old' unit. This provides a powerful tool to test how process modifications and storage conditions affect transfused platelet function in vivo.
    Language English
    Publishing date 2024-04-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 80313-3
    ISSN 1423-0410 ; 0042-9007
    ISSN (online) 1423-0410
    ISSN 0042-9007
    DOI 10.1111/vox.13642
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article: Characterization of a Novel Mouse Platelet Transfusion Model.

    Gordy, Dominique / Swayne, Theresa / Berry, Gregory J / Thomas, Tiffany A / Hudson, Krystalyn E / Stone, Elizabeth F

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Background: Platelet transfusions are increasing with advances in medical care. Based on FDA criteria, platelet units are assessed by : Study design and methods: Platelet units stored in mouse plasma were prepared using a modified platelet rich ... ...

    Abstract Background: Platelet transfusions are increasing with advances in medical care. Based on FDA criteria, platelet units are assessed by
    Study design and methods: Platelet units stored in mouse plasma were prepared using a modified platelet rich plasma collection protocol. Characteristics of fresh and stored units, including pH, cell count,
    Results: Platelet units had acceptable platelet and white cell counts and were negative for bacterial contamination. Fresh and 1-day stored units had acceptable pH; the platelets were activatable by thrombin and ADP, aggregable with thrombin, had acceptable PTR, and were present
    Discussion: We developed mouse platelets for transfusion analogous to human platelet units using a modified platelet rich plasma collection protocol with maximum storage of 1 day for an "old" unit. This provides a powerful tool to test how process modifications and storage conditions affect transfused platelet function
    Language English
    Publishing date 2023-11-14
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.11.10.566577
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Reticulocytes in donor RBC units enhance RBC alloimmunization.

    Thomas, Tiffany A / Qiu, Annie / Kim, Christopher Y / Gordy, Dominique E / Miller, Anabel / Tredicine, Maria / Dzieciatkowska, Monika / Zotti, Flavia Dei / Hod, Eldad A / Dâ Alessandro, Angelo / Zimring, James C / Spitalnik, Steven L / Hudson, Krystalyn E

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Although red blood cell (RBC) transfusions save lives, some patients develop clinically-significant alloantibodies against donor blood group antigens, which then have adverse effects in multiple clinical settings. Few effective measures exist to prevent ... ...

    Abstract Although red blood cell (RBC) transfusions save lives, some patients develop clinically-significant alloantibodies against donor blood group antigens, which then have adverse effects in multiple clinical settings. Few effective measures exist to prevent RBC alloimmunization and/or eliminate alloantibodies in sensitized patients. Donor-related factors may influence alloimmunization; thus, there is an unmet clinical need to identify which RBC units are immunogenic. Repeat volunteer blood donors and donors on iron supplements have elevated reticulocyte counts compared to healthy non-donors. Early reticulocytes retain mitochondria and other components, which may act as danger signals in immune responses. Herein, we tested whether reticulocytes in donor RBC units could enhance RBC alloimmunization. Using a murine model, we demonstrate that transfusing donor RBC units with increased reticulocyte frequencies dose-dependently increase RBC alloimmunization rates and alloantibody levels. Transfusing reticulocyte-rich RBC units was associated with increased RBC clearance from the circulation and a robust proinflammatory cytokine response. As compared to previously reported post-transfusion RBC consumption patterns, erythrophagocytosis from reticulocyte-rich units was increasingly performed by splenic B cells. These data suggest that reticulocytes in a donated RBC unit impact the quality of blood transfused, are targeted to a distinct compartment, and may be an underappreciated risk factor for RBC alloimmunization.
    Language English
    Publishing date 2023-01-25
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.01.25.525560
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Reticulocytes in donor blood units enhance red blood cell alloimmunization.

    Thomas, Tiffany A / Qiu, Annie / Kim, Christopher Y / Gordy, Dominique E / Miller, Anabel / Tredicine, Maria / Dzieciatkowska, Monika / Dei Zotti, Flavia / Hod, Eldad A / D'Alessandro, Angelo / Zimring, James C / Spitalnik, Steven L / Hudson, Krystalyn E

    Haematologica

    2023  Volume 108, Issue 10, Page(s) 2639–2651

    Abstract: Although red blood cell (RBC) transfusions save lives, some patients develop clinically-significant alloantibodies against donor blood group antigens, which then have adverse effects in multiple clinical settings. Few effective measures exist to prevent ... ...

    Abstract Although red blood cell (RBC) transfusions save lives, some patients develop clinically-significant alloantibodies against donor blood group antigens, which then have adverse effects in multiple clinical settings. Few effective measures exist to prevent RBC alloimmunization and/or eliminate alloantibodies in sensitized patients. Donor-related factors may influence alloimmunization; thus, there is an unmet clinical need to identify which RBC units are immunogenic. Repeat volunteer blood donors and donors on iron supplements have elevated reticulocyte counts compared to healthy non-donors. Early reticulocytes retain mitochondria and other components, which may act as danger signals in immune responses. Herein, we tested whether reticulocytes in donor RBC units could enhance RBC alloimmunization. Using a murine model, we demonstrate that transfusing donor RBC units with increased reticulocyte frequencies dose-dependently increased RBC alloimmunization rates and alloantibody levels. Transfusing reticulocyte-rich RBC units was associated with increased RBC clearance from the circulation and a robust proinflammatory cytokine response. As compared to previously reported post-transfusion RBC consumption patterns, erythrophagocytosis from reticulocyte-rich units was increasingly performed by splenic B cells. These data suggest that reticulocytes in a donated RBC unit impact the quality of blood transfused, are targeted to a distinct compartment, and may be an underappreciated risk factor for RBC alloimmunization.
    MeSH term(s) Humans ; Mice ; Animals ; Reticulocytes ; Isoantibodies ; Blood Donors ; Erythrocytes ; Risk Factors
    Chemical Substances Isoantibodies
    Language English
    Publishing date 2023-10-01
    Publishing country Italy
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2333-4
    ISSN 1592-8721 ; 0017-6567 ; 0390-6078
    ISSN (online) 1592-8721
    ISSN 0017-6567 ; 0390-6078
    DOI 10.3324/haematol.2023.282815
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.76: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  5. Article ; Online: Low-Dose Dietary Fish Oil Improves RBC Deformability without Improving Post-Transfusion Recovery in Mice.

    Kim, Christopher Y / Larsen, Hannah J / Spitalnik, Steven L / Hod, Eldad A / Francis, Richard O / Hudson, Krystalyn E / Gordy, Dominique E / Stone, Elizabeth F / Peltier, Sandy / Amireault, Pascal / D'Alessandro, Angelo / Zimring, James C / Buehler, Paul W / Fu, Xiaoyun / Thomas, Tiffany

    Nutrients

    2023  Volume 15, Issue 20

    Abstract: Long-chain polyunsaturated fatty acids (LC-PUFAs) are important modulators of red blood cell (RBC) rheology. Dietary LC-PUFAs are readily incorporated into the RBC membrane, improving RBC deformability, fluidity, and hydration. Female C57BL/6J mice ... ...

    Abstract Long-chain polyunsaturated fatty acids (LC-PUFAs) are important modulators of red blood cell (RBC) rheology. Dietary LC-PUFAs are readily incorporated into the RBC membrane, improving RBC deformability, fluidity, and hydration. Female C57BL/6J mice consumed diets containing increasing amounts of fish oil (FO) ad libitum for 8 weeks. RBC deformability, filterability, and post-transfusion recovery (PTR) were evaluated before and after cold storage. Lipidomics and lipid peroxidation markers were evaluated in fresh and stored RBCs. High-dose dietary FO (50%, 100%) was associated with a reduction in RBC quality (i.e., in vivo lifespan, deformability, lipid peroxidation) along with a reduced 24 h PTR after cold storage. Low-dose dietary FO (6.25-12.5%) improved the filterability of fresh RBCs and reduced the lipid peroxidation of cold-stored RBCs. Although low doses of FO improved RBC deformability and reduced oxidative stress, no improvement was observed for the PTR of stored RBCs. The improvement in RBC deformability observed with low-dose FO supplementation could potentially benefit endurance athletes and patients with conditions resulting from reduced perfusion, such as peripheral vascular disease.
    MeSH term(s) Humans ; Female ; Mice ; Animals ; Mice, Inbred C57BL ; Erythrocyte Deformability ; Erythrocytes/metabolism ; Fish Oils/pharmacology ; Fish Oils/metabolism ; Fatty Acids, Unsaturated/metabolism ; Fatty Acids/metabolism ; Dietary Fats, Unsaturated/metabolism ; Blood Preservation/methods
    Chemical Substances Fish Oils ; Fatty Acids, Unsaturated ; Fatty Acids ; Dietary Fats, Unsaturated
    Language English
    Publishing date 2023-10-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu15204456
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Domestic and International Shipping of Biospecimens.

    Gordy, Dominique / Tashjian, Randy S / Lee, Hannah / Movassaghi, Masoud / Yong, William H

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1897, Page(s) 433–443

    Abstract: The packaging and shipment of biospecimens is a multistep process for which a distinct set of regulations needs to be followed, depending on whether a biospecimen is shipped domestically or internationally and whether the shipment contains hazardous ... ...

    Abstract The packaging and shipment of biospecimens is a multistep process for which a distinct set of regulations needs to be followed, depending on whether a biospecimen is shipped domestically or internationally and whether the shipment contains hazardous materials. Shipments may be delayed if these regulations are not followed. Once learned, the process is straightforward. Major principles include double or triple packaging, adequate absorbent material, appropriate coolant, accurate labeling, and complete documentation. Training in packaging and shipping is often offered at major biomedical institutions and is a requirement to avoid shipping biohazards.
    MeSH term(s) Biological Specimen Banks/standards ; Humans ; Specimen Handling ; Transportation/methods
    Language English
    Publishing date 2018-12-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-8935-5_35
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Deuterated Linoleic Acid Attenuates the RBC Storage Lesion in a Mouse Model of Poor RBC Storage.

    Kim, Christopher Y / Johnson, Hannah / Peltier, Sandy / Spitalnik, Steven L / Hod, Eldad A / Francis, Richard O / Hudson, Krystalyn E / Stone, Elizabeth F / Gordy, Dominique E / Fu, Xiaoyun / Zimring, James C / Amireault, Pascal / Buehler, Paul W / Wilson, Robert B / D'Alessandro, Angelo / Shchepinov, Mikhail S / Thomas, Tiffany

    Frontiers in physiology

    2022  Volume 13, Page(s) 868578

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2022-04-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2022.868578
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Neuropathology of COVID-19 (neuro-COVID): clinicopathological update.

    Lou, Jerry J / Movassaghi, Mehrnaz / Gordy, Dominique / Olson, Madeline G / Zhang, Ting / Khurana, Maya S / Chen, Zesheng / Perez-Rosendahl, Mari / Thammachantha, Samasuk / Singer, Elyse J / Magaki, Shino D / Vinters, Harry V / Yong, William H

    Free neuropathology

    2020  Volume 2

    Abstract: Coronavirus disease 2019 (COVID-19) is emerging as the greatest public health crisis in the early ... ...

    Abstract Coronavirus disease 2019 (COVID-19) is emerging as the greatest public health crisis in the early 21
    Language English
    Publishing date 2020-02-29
    Publishing country Canada
    Document type Journal Article
    ISSN 2699-4445
    ISSN (online) 2699-4445
    DOI 10.17879/freeneuropathology-2021-2993
    Database MEDical Literature Analysis and Retrieval System OnLINE

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