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  1. Article ; Online: Validated High-Performance Thin-Layer Chromatography-Mass Spectrometry Method and Stability Study of Linalool in the Volatile Oil of the Rhizomes of Homalomena aromatica Schott.

    Goswami, Ashis Kumar / Gogoi, Neelutpal / Sharma, Hemanta Kumar

    Journal of chromatographic science

    2022  Volume 61, Issue 3, Page(s) 269–278

    Abstract: Homalomena aromatica is a herb of tremendous ethnomedicinal importance to various communities residing in northeast India. In this study, a high-performance thin-layer chromatography-based densitometric method was developed for identification, ... ...

    Abstract Homalomena aromatica is a herb of tremendous ethnomedicinal importance to various communities residing in northeast India. In this study, a high-performance thin-layer chromatography-based densitometric method was developed for identification, quantification and stability study of linalool. Mass spectrometry was hyphenated to HPTLC for streamlining the method. The stability of linalool was studied by analyzing the effect of acid, base, UV, sunlight, thermal stress and H2O2 on linalool. The chromatographic plates were developed to a height of 70 mm in toluene:ethyl acetate solvent system at a ratio of 9.5:0.5 and visualized with p-anisaldehyde reagent. The developed method was found to be precise, accurate and reproducible according to International Conference on Harmonization guidelines, and compact bands of linalool were observed at Rf of 0.351 ± 0.001. The content of linalool in the volatile oil of H. aromatica was found to be 58% v/v. By application of the hyphenated MS technique, linalool was identified at m/z 137, (M + H)+. It was observed that acidic pH has the highest effect on linalool with a percentage degradation of 65. The developed method can be used in the analysis and quality control of herbal materials and volatile oils containing linalool and quality control of rhizomes of H. aromatica.
    MeSH term(s) Oils, Volatile/analysis ; Chromatography, Thin Layer/methods ; Rhizome/chemistry ; Hydrogen Peroxide ; Mass Spectrometry
    Chemical Substances Oils, Volatile ; linalool (D81QY6I88E) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2022-03-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80141-0
    ISSN 1945-239X ; 0021-9665
    ISSN (online) 1945-239X
    ISSN 0021-9665
    DOI 10.1093/chromsci/bmac012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 895: Show issues Location:
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  2. Article: Integrated computational approach towards repurposing of antimalarial drug against SARS-CoV-2 main protease.

    Gogoi, Neelutpal / Chowdhury, Purvita / Goswami, Ashis Kumar / Das, Aparoop / Chetia, Dipak / Gogoi, Bhaskarjyoti

    Structural chemistry

    2022  Volume 33, Issue 5, Page(s) 1409–1422

    Abstract: Huge vaccination drives are underway around the world for the ongoing COVID-19 pandemic. However, the search for antiviral drugs is equally crucial. As new drug discovery is a time-consuming process, repurposing of existing drugs or developing drug ... ...

    Abstract Huge vaccination drives are underway around the world for the ongoing COVID-19 pandemic. However, the search for antiviral drugs is equally crucial. As new drug discovery is a time-consuming process, repurposing of existing drugs or developing drug candidates against SARS-CoV-2 will make the process faster. Considering this, 63 approved and developing antimalarial compounds were selected to screen against main protease (M
    Supplementary information: The online version contains supplementary material available at 10.1007/s11224-022-01916-0.
    Language English
    Publishing date 2022-05-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2018832-8
    ISSN 1572-9001 ; 1040-0400
    ISSN (online) 1572-9001
    ISSN 1040-0400
    DOI 10.1007/s11224-022-01916-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: In vitro Evaluation and Molecular Dynamics, DFT Guided Investigation of Antimalarial Activity of Ethnomedicinally used Coptis teeta Wall.

    Goswami, Ashis Kumar / Sharma, Hemanta Kumar / Gogoi, Neelutpal / Kashyap, Ankita / Gogoi, Bhaskarjyoti

    Combinatorial chemistry & high throughput screening

    2021  Volume 25, Issue 2, Page(s) 292–306

    Abstract: Background: Malaria is caused by different species of Plasmodium; among which P. falciparum is the most severe. Coptis teeta is an ethnomedicinal plant of enormous importance for tribes of northeast India.: Objective: In this study, the antimalarial ... ...

    Abstract Background: Malaria is caused by different species of Plasmodium; among which P. falciparum is the most severe. Coptis teeta is an ethnomedicinal plant of enormous importance for tribes of northeast India.
    Objective: In this study, the antimalarial activity of the methanol extracts of Coptis teeta was evaluated in vitro and lead identification was carried out via in silico study.
    Methods: On the basis of the in vitro results, in silico analysis by application of different modules of Discovery Studio 2018 was performed on multiple targets of P. falciparum taking into consideration some of the compounds reported from C. teeta.
    Results: The IC50 of the methanol extract of Coptis teeta was reported to be 0.08 μg/ml in 3D7 strain and 0.7 μg/ml in Dd2 strain of P. falciparum. From the docking study, noroxyhydrastatine was observed to have better binding affinity in comparison to chloroquine. The binding of noroxyhydrastinine with dihydroorotate dehydrogenase was further validated by molecular dynamics simulation and was observed to be significantly stable in comparison to the co-crystal inhibitor. During simulations, it was observed that noroxyhydrastinine retained the interactions, giving strong indications of its effectiveness against the P. falciparum proteins and stability in the binding pocket. From the Density-functional theory analysis, the bandgap energy of noroxyhydrastinine was found to be 0.186 Ha, indicating a favorable interaction.
    Conclusion: The in silico analysis as an addition to the in vitro results provides strong evidence of noroxyhydrastinine as an antimalarial agent.
    MeSH term(s) Antimalarials/chemistry ; Antimalarials/pharmacology ; Coptis/chemistry ; Molecular Dynamics Simulation ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plasmodium falciparum
    Chemical Substances Antimalarials ; Plant Extracts
    Language English
    Publishing date 2021-01-19
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2064785-2
    ISSN 1875-5402 ; 1386-2073
    ISSN (online) 1875-5402
    ISSN 1386-2073
    DOI 10.2174/1386207324666210118095503
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5577: Show issues Location:
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  4. Article ; Online: Development and Validation of High-Performance Thin-layer Chromatographic Method for Quantification of Berberine in Rhizomes of Coptis teeta Wall, an Endangered Species Collected from Arunachal Pradesh, India.

    Goswami, Ashis Kumar / Gogoi, Neelutpal / Shakya, Anshul / Sharma, Hemanta Kumar

    Journal of chromatographic science

    2019  Volume 57, Issue 5, Page(s) 411–417

    Abstract: The high polarity of the protoberberine alkaloids present in Coptis teeta has made it difficult to quantify the alkaloids. This study was designed to develop and validate a thin-layer chromatography (TLC) densitometric-based method using high-performance ...

    Abstract The high polarity of the protoberberine alkaloids present in Coptis teeta has made it difficult to quantify the alkaloids. This study was designed to develop and validate a thin-layer chromatography (TLC) densitometric-based method using high-performance thin-layer chromatography for quantification of berberine. The separation was achieved in a solvent system consisting of butanol:ethyl acetate:formic acid:water by volume on TLC aluminum plate precoated with silica gel 60 F254. Determination and quantification were performed by densitometric scanning under mercury lamp at a wavelength 351 nm in absorbance mode. The validated method gave compact bands for berberine at an Rf of 0.70. The precision, accuracy and reproducibility of the method were validated by following International Conference on Harmonization guidelines. Graphically, linear results were obtained for berberine with correlation coefficient of 0.997 ± 0.09% (R ± SD) in the concentration range of 90-210 ng/band. The limit of quantification and limit of detection from the analysis were found to be 70 and 30 ng/band, respectively. The berberine concentration in the methanol extract of C. teeta was found to be 30.97 ± 0.55 mg in 100 mg of the crude drug. The method developed here in can be implemented in the analysis and routine quality control of herbal materials and formulations containing C. teeta and berberine.
    MeSH term(s) Berberine/analysis ; Berberine/isolation & purification ; Chemical Fractionation ; Chromatography, Thin Layer/methods ; Coptis/chemistry ; Densitometry/methods ; Endangered Species ; India ; Plant Extracts/analysis ; Plant Extracts/isolation & purification ; Reproducibility of Results ; Rhizome/chemistry
    Chemical Substances Plant Extracts ; Berberine (0I8Y3P32UF)
    Language English
    Publishing date 2019-02-12
    Publishing country United States
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 80141-0
    ISSN 1945-239X ; 0021-9665
    ISSN (online) 1945-239X
    ISSN 0021-9665
    DOI 10.1093/chromsci/bmz009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 895: Show issues Location:
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  5. Article ; Online: Computational guided identification of a citrus flavonoid as potential inhibitor of SARS-CoV-2 main protease.

    Gogoi, Neelutpal / Chowdhury, Purvita / Goswami, Ashis Kumar / Das, Aparoop / Chetia, Dipak / Gogoi, Bhaskarjyoti

    Molecular diversity

    2020  Volume 25, Issue 3, Page(s) 1745–1759

    Abstract: Although vaccine development is being undertaken at a breakneck speed, there is currently no effective antiviral drug for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing COVID-19. Therefore, the present study aims to explore the ... ...

    Abstract Although vaccine development is being undertaken at a breakneck speed, there is currently no effective antiviral drug for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing COVID-19. Therefore, the present study aims to explore the possibilities offered by naturally available and abundant flavonoid compounds, as a prospective antiviral drug to combat the virus. A library of 44 citrus flavonoids was screened against the highly conserved Main Protease (M
    MeSH term(s) Citrus/chemistry ; Coronavirus 3C Proteases/antagonists & inhibitors ; Coronavirus 3C Proteases/chemistry ; Coronavirus 3C Proteases/metabolism ; Drug Discovery ; Flavonoids/metabolism ; Flavonoids/pharmacology ; Molecular Docking Simulation ; Protease Inhibitors/metabolism ; Protease Inhibitors/pharmacology ; Protein Conformation ; Quercetin/analogs & derivatives ; Quercetin/metabolism ; Quercetin/pharmacology ; SARS-CoV-2/drug effects ; SARS-CoV-2/enzymology
    Chemical Substances Flavonoids ; Protease Inhibitors ; Quercetin (9IKM0I5T1E) ; taxifolin (9SOB9E3987) ; Coronavirus 3C Proteases (EC 3.4.22.28)
    Language English
    Publishing date 2020-11-25
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1376507-3
    ISSN 1573-501X ; 1381-1991
    ISSN (online) 1573-501X
    ISSN 1381-1991
    DOI 10.1007/s11030-020-10150-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4946: Show issues Location:
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  6. Article: Development and Validation of High-Performance Thin-layer Chromatographic Method for Quantification of Berberine in Rhizomes of Coptis teeta Wall, an Endangered Species Collected from Arunachal Pradesh, India

    Goswami, Ashis Kumar / Gogoi, Neelutpal / Shakya, Anshul / Sharma, Hemanta Kumar

    Journal of chromatographic science. 2019 May 01, v. 57, no. 5

    2019  

    Abstract: The high polarity of the protoberberine alkaloids present in Coptis teeta has made it difficult to quantify the alkaloids. This study was designed to develop and validate a thin-layer chromatography (TLC) densitometric-based method using high-performance ...

    Abstract The high polarity of the protoberberine alkaloids present in Coptis teeta has made it difficult to quantify the alkaloids. This study was designed to develop and validate a thin-layer chromatography (TLC) densitometric-based method using high-performance thin-layer chromatography for quantification of berberine. The separation was achieved in a solvent system consisting of butanol:ethyl acetate:formic acid:water by volume on TLC aluminum plate precoated with silica gel 60 F₂₅₄. Determination and quantification were performed by densitometric scanning under mercury lamp at a wavelength 351 nm in absorbance mode. The validated method gave compact bands for berberine at an Rf of 0.70. The precision, accuracy and reproducibility of the method were validated by following International Conference on Harmonization guidelines. Graphically, linear results were obtained for berberine with correlation coefficient of 0.997 ± 0.09% (R ± SD) in the concentration range of 90–210 ng/band. The limit of quantification and limit of detection from the analysis were found to be 70 and 30 ng/band, respectively. The berberine concentration in the methanol extract of C. teeta was found to be 30.97 ± 0.55 mg in 100 mg of the crude drug. The method developed here in can be implemented in the analysis and routine quality control of herbal materials and formulations containing C. teeta and berberine.
    Keywords Coptis ; absorbance ; aluminum ; berberine ; densitometry ; detection limit ; drugs ; endangered species ; guidelines ; lamps ; methanol ; quality control ; rhizomes ; silica gel ; solvents ; thin layer chromatography ; wavelengths ; India
    Language English
    Dates of publication 2019-0501
    Size p. 411-417.
    Publishing place Oxford University Press
    Document type Article
    ZDB-ID 80141-0
    ISSN 1945-239X ; 0021-9665
    ISSN (online) 1945-239X
    ISSN 0021-9665
    DOI 10.1093/chromsci/bmz009
    Database NAL-Catalogue (AGRICOLA)

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