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  1. Article ; Online: Identification of Escherichia coli isolated from flies (Insecta: Diptera) that inhabit the environment of dairy farms harboring extraintestinal virulence markers.

    Motta, Rodrigo G / Motta, Lorrayne S A M / Bertolini, Amanda B / Prado, Aline M / Thyssen, Patricia J / Hernandes, Rodrigo T / Orsi, Henrique / Rall, Vera L M / Gouvea, Fábio L R / Guerra, Simony T / Guimarães, Felipe F / Joaquim, Sâmea F / Pantoja, José C / Langoni, Helio / Ribeiro, Márcio G

    Journal of applied microbiology

    2023  Volume 134, Issue 12

    Abstract: Aims: We investigate extraintestinal pathogenic genes (ExPEC) related to virulence of Escherichia coli in flies from the dairy environment.: Methods and results: We collected 217 flies from nine dairy farms, which were submitted to microbiological ... ...

    Abstract Aims: We investigate extraintestinal pathogenic genes (ExPEC) related to virulence of Escherichia coli in flies from the dairy environment.
    Methods and results: We collected 217 flies from nine dairy farms, which were submitted to microbiological culture. Fifty-one E. coli were identified using mass spectrometry. Eleven dipteran families were identified, with a predominance of Muscidae, and a minor frequency of Tachinidae, Drosophilidae, Sphaeroceridae, Ulidiidae, Syrphidae, Chloropidae, Calliphoridae, Sarcophagidae, and Piophilidae. A panel of 16 virulence-encoding genes related to ExPEC infections were investigated, which revealed predominance of serum resistance (traT, 31/51 = 60.8%; ompT, 29/51 = 56.9%), iron uptake (irp2, 17/51 = 33.3%, iucD 11/51 = 21.6%), and adhesins (papC, 6/51 = 11.8%; papA, 5/51 = 9.8%).
    Conclusions: Our findings reveal Dipterans from milking environment carrying ExPEC virulence-encoding genes also identified in clinical bovine E. coli-induced infections.
    MeSH term(s) Humans ; Animals ; Cattle ; Escherichia coli/genetics ; Virulence/genetics ; Farms ; Escherichia coli Infections/veterinary ; Escherichia coli Infections/microbiology ; Virulence Factors/genetics ; Extraintestinal Pathogenic Escherichia coli ; Insecta ; Diptera
    Chemical Substances Virulence Factors
    Language English
    Publishing date 2023-12-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1358023-1
    ISSN 1365-2672 ; 1364-5072
    ISSN (online) 1365-2672
    ISSN 1364-5072
    DOI 10.1093/jambio/lxad301
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 259: Show issues Location:
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  2. Article ; Online: Management of massive hemopericardium in the electrophysiology laboratory: The double long sheath technique.

    Chokr, Muhieddine Omar / Santos, Italo Bruno Dos Santos Sousa / Gouvea, Fabio Cesar / Kulchetscki, Rodrigo / Andere, Tamer El / Hardy, Carina / Pisani, Cristiano / Melo, Sissy / Scanavacca, Mauricio

    Pacing and clinical electrophysiology : PACE

    2022  Volume 45, Issue 5, Page(s) 583–588

    Abstract: Aim: To describe a simple and useful technique for acute management of massive hemopericardium inside the Electrophysiology (EP) laboratory METHODS AND RESULTS: Five patients from a single center experience were identified, all with blood loss above ... ...

    Abstract Aim: To describe a simple and useful technique for acute management of massive hemopericardium inside the Electrophysiology (EP) laboratory METHODS AND RESULTS: Five patients from a single center experience were identified, all with blood loss above 1000 ml after initial pericardiocenthesis. Using two long 8.5 F transseptal sheaths inside the pericardium space, with continuous negative pressure, allowed the complete cessation of bleeding or hemodynamic maintenance until definitive surgical repair in all patients CONCLUSION: The use of two long sheaths for blood drainage, instead of conventional pericardiocenthesis, might be helpful to manage massive hemopericardium inside EP lab, avoiding urgent cardiac surgery or maintaining clinical stability until surgical staff is available.
    MeSH term(s) Cardiac Electrophysiology ; Cardiac Surgical Procedures ; Humans ; Pericardial Effusion/surgery ; Pericardium/surgery
    Language English
    Publishing date 2022-03-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 424437-0
    ISSN 1540-8159 ; 0147-8389
    ISSN (online) 1540-8159
    ISSN 0147-8389
    DOI 10.1111/pace.14477
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Left Atrial Thrombus and Dense Spontaneous Contrast in Direct Oral Anticoagulant Therapy of Atrial Fibrillation: Insights from a Reference Center.

    Marques, Thiago / Darrieux, Francisco / Gouvêa, Fábio / Garambone, Leandro / Lindoso, Ana Paula / Lage, João / Sacilotto, Luciana / Coimbra, Ana Lúcia / Pinheiro, Martina / Olivetti, Natália / Lara, Sissy / Hardy, Carina / Athayde, Guilherme / Hachul, Denise / Pisani, Cristiano / Wu, Tan Chen / Scanavacca, Maurício

    Arquivos brasileiros de cardiologia

    2022  Volume 119, Issue 4, Page(s) 514–519

    Abstract: Background: In the treatment of atrial fibrillation (AF), the most frequently sustained arrhythmia, with catheter ablation (CA) or electrical cardioversion (ECV), the periprocedural period is one of the most critical phases. Currently, the use of new ... ...

    Title translation Trombo Atrial Esquerdo e Contraste Espontâneo Denso no Uso de Anticoagulante Oral de Ação Direta em Fibrilação Atrial: Visão de Centro Referenciado.
    Abstract Background: In the treatment of atrial fibrillation (AF), the most frequently sustained arrhythmia, with catheter ablation (CA) or electrical cardioversion (ECV), the periprocedural period is one of the most critical phases. Currently, the use of new direct action oral anticoagulants (DOAC) is increasingly frequent; however, in the real world, there are still few data on studies on the thrombus incidence in the left atrium (TrLA) or dense spontaneous contrast (DSC) on transesophageal echocardiogram (TEE).
    Objective: To evaluate the prevalence of events and association with risk factors in patients using DOACs. Primary objective: to analyze the prevalence of thrombus in the LA by TEE in patients using DOAC undergoing ECV/CA. Second, evaluate the association of comorbidities with the presence of thrombi and DSC.
    Methods: Retrospective cohort, single-center study with patients followed at the Arrhythmia Outpatient Unit (InCor-HCFMUSP). Patients indicated for procedures and using DOACs were selected, and their clinical/echocardiographic data were analyzed. A significance level of 5% was considered.
    Results: 354 patients were included, a total of 400 procedures, from March 2012-March 2018. Thrombus in the LA was found in 11 patients (2.8%), associated with advanced age (p=0.007) and higher CHA2DS2-VASc (p<0.001) score. DSC in the LA before TEE was found in 29 patients (7.3%), with lower LVEF (p<0.038) and greater LA dimension (p<0.0001).
    Conclusion: The incidence of LA thrombus and DSC in patients using DOC in the context of AF ECV/CA, although small, is not negligible. Patients with higher CHA2DS2-VASc scores, especially older and with larger LA diameter, are more prone to these echocardiographic findings.
    MeSH term(s) Humans ; Atrial Fibrillation ; Retrospective Studies ; Administration, Oral ; Anticoagulants/therapeutic use ; Thrombosis/diagnostic imaging ; Thrombosis/epidemiology ; Thrombosis/etiology ; Heart Diseases/diagnostic imaging ; Heart Diseases/epidemiology ; Echocardiography, Transesophageal ; Atrial Appendage/diagnostic imaging
    Chemical Substances Anticoagulants
    Language Portuguese
    Publishing date 2022-10-20
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 730261-7
    ISSN 1678-4170 ; 0066-782X
    ISSN (online) 1678-4170
    ISSN 0066-782X
    DOI 10.36660/abc.20210658
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 548: Show issues Location:
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  4. Article ; Online: Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus.

    Bertolini, Amanda Bezerra / Thyssen, Patrícia Jacqueline / Brandão, Paulo Eduardo / Prado, Aline Marrara / Silva, Sheila Oliveira de Souza / Mioni, Mateus de Souza Ribeiro / de Gouvea, Fábio Lucas Rezende / Pantoja, José Carlos de Figueiredo / Langoni, Helio / Guimarães, Felipe Freitas / Joaquim, Sâmea Fernandes / Guerra, Simony Trevizan / Leite, Domingos da Silva / Rall, Vera Mores / Hernandes, Rodrigo Tavanelli / Lucheis, Simone Baldini / Rossi, Gabriel Augusto Marques / Ribeiro, Márcio Garcia

    Journal of applied microbiology

    2023  Volume 134, Issue 3

    Abstract: Aims: We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms.: Methods and results: We collected 217 insect specimens from nine dairy farms, which were examined through ... ...

    Abstract Aims: We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms.
    Methods and results: We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens.
    Conclusions: Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.
    MeSH term(s) Animals ; Cattle ; Rotavirus/genetics ; Rotavirus Infections ; Diptera ; Betacoronavirus ; Farms ; Insecta ; Feces ; Cattle Diseases/epidemiology ; Diarrhea/epidemiology ; Phylogeny ; Genotype
    Language English
    Publishing date 2023-02-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 1358023-1
    ISSN 1365-2672 ; 1364-5072
    ISSN (online) 1365-2672
    ISSN 1364-5072
    DOI 10.1093/jambio/lxad020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 259: Show issues Location:
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