Artikel ; Online: S100A7 in Psoriasis: Immunodetection and Activation by CRISPR technology.
Methods in molecular biology (Clifton, N.J.)
2019 Band 1929, Seite(n) 729–738
Abstract: Psoriasis, an inflammatory autoimmune skin disease, is the result of a chronic interaction between hyperproliferative keratinocytes and infiltrating activated immune cells. The mechanisms underlying psoriasis pathogenesis remain largely unknown, although ...
| Abstract | Psoriasis, an inflammatory autoimmune skin disease, is the result of a chronic interaction between hyperproliferative keratinocytes and infiltrating activated immune cells. The mechanisms underlying psoriasis pathogenesis remain largely unknown, although a combination of genetic and environmental factors plays an important role in its development. S100A7 is overexpressed in psoriasis, and there is growing evidence that S100A7 may be involved in the pathogenesis of psoriasis. Since the mechanisms underlying S100A7 regulation and function remain elusive, a better understanding of these mechanisms may be useful to uncover additional treatment approaches for psoriasis. Immunohistology provides invaluable tools for a better understanding of the pathogenetic mechanisms of psoriasis. Here, we describe basic methods for immunofluorescence and immunohistochemistry analysis of S100A7 expression in psoriatic patients as well as in S100A7 CRISPR-activated human keratinocyte cell line. |
|---|---|
| Mesh-Begriff(e) | COP9 Signalosome Complex/metabolism ; CRISPR-Cas Systems ; Cell Line ; Fluorescent Antibody Technique ; Humans ; Intracellular Signaling Peptides and Proteins/metabolism ; Keratinocytes/cytology ; Keratinocytes/metabolism ; Peptide Hydrolases/metabolism ; Psoriasis/genetics ; Psoriasis/metabolism ; S100 Calcium Binding Protein A7/genetics ; S100 Calcium Binding Protein A7/metabolism ; Transcriptional Activation ; Up-Regulation |
| Chemische Substanzen | Intracellular Signaling Peptides and Proteins ; S100 Calcium Binding Protein A7 ; S100A7 protein, human ; Peptide Hydrolases (EC 3.4.-) ; COPS5 protein, human (EC 3.4.-.-) ; COP9 Signalosome Complex (EC 3.4.19.12) |
| Sprache | Englisch |
| Erscheinungsdatum | 2019-02-01 |
| Erscheinungsland | United States |
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1940-6029 |
| ISSN (online) | 1940-6029 |
| DOI | 10.1007/978-1-4939-9030-6_45 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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