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  1. Book ; Online: Molecular and Metabolic Mechanisms Associated with Fleshy Fruit Quality

    Pezzotti, Mario / Granell, Antonio / Fortes, Ana M. / Bouzayen, Mondher

    2017  

    Abstract: Fleshy Fruits are a late acquisition of plant evolution. In addition of protecting the seeds, these specialized organs unique to plants were developed to promote seed dispersal via the contribution of frugivorous animals. Fruit development and ripening ... ...

    Abstract Fleshy Fruits are a late acquisition of plant evolution. In addition of protecting the seeds, these specialized organs unique to plants were developed to promote seed dispersal via the contribution of frugivorous animals. Fruit development and ripening is a complex process and understanding the underlying genetic and molecular program is a very active field of research. Part of the ripening process is directed to build up quality traits such as color, texture and aroma that make the fruit attractive and palatable. As fruit consumers, humans have developed a time long interaction with fruits which contributed to make the fruit ripening attributes conform our needs and preferences. This issue of Frontiers in Plant Science is intended to cover the most recent advances in our understanding of different aspects of fleshy fruit biology, including the genetic, molecular and metabolic mechanisms associated to each of the fruit quality traits.-

    It is also of prime importance to consider the effects of environmental cues, cultural practices and postharvest methods, and to decipher the mechanism by which they impact fruit quality traits. Most of our knowledge of fleshy fruit development, ripening and quality traits comes from work done in a reduced number of species that are not only of economic importance but can also benefit from a number of genetic and genomic tools available to their specific research communities. For instance, working with tomato and grape offers several advantages since the genome sequences of these two fleshy fruit species have been deciphered and a wide range of biological and genetic resources have been developed. Ripening mutants are available for tomato which constitutes the main model system for fruit functional genomics. In addition, tomato is used as a reference species for climacteric fruit which ripening is controlled by the phytohormone ethylene.-

    Likewise, grape is a reference species for non-climacteric fruit even though no single master switches controlling ripening initiation have been uncovered yet. In the last period, the genome sequence of an increased number of fruit crop species became available which creates a suitable situation for research communities around crops to get organized and information to be shared through public repositories. On the other hand, the availability of genome-wide expression profiling technologies has enabled an easier study of global transcriptional changes in fruit species where the sequenced genome is not yet available. In this issue authors will present recent progress including original data as well as authoritative reviews on our understanding of fleshy fruit biology focusing on tomato and grape as model species
    Keywords Science (General) ; Botany ; Physiology
    Size 1 electronic resource (436 p.)
    Publisher Frontiers Media SA
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT020097413
    ISBN 9782889452729 ; 2889452727
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book ; Online: Genetics and Genomics of Plant Reproduction for Crop Breeding

    Barcaccia, Gianni / Baldoni, Luciana / Adelina Mendes, Marta / Albertini, Emidio / Pupilli, Fulvio / Mazzucato, Andrea / Zenoni, Sara / Vieira Coimbra, Silvia / Granell, Antonio / Zhang, Dabing

    2020  

    Keywords Science: general issues ; Botany & plant sciences ; Gametes ; Male-Sterility ; Self-Incompatibility ; Parthenocarpy ; Parthenogenesis ; Apomixis
    Size 1 electronic resource (377 pages)
    Publisher Frontiers Media SA
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021230619
    ISBN 9782889638871 ; 2889638871
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  3. Article ; Online: Determination of Plant Volatile Apocarotenoids.

    Rambla, José L / Granell, Antonio

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 2083, Page(s) 165–175

    Abstract: Volatile compounds derived from carotenoid cleavage are biologically relevant molecules in plants, as they are involved in different processes of plant communication and also have a significant effect on our perception of food flavor.Here we describe two ...

    Abstract Volatile compounds derived from carotenoid cleavage are biologically relevant molecules in plants, as they are involved in different processes of plant communication and also have a significant effect on our perception of food flavor.Here we describe two methods for the determination of volatile apocarotenoids in fruit based on extraction by means of headspace solid-phase microextraction (HS-SPME) and separation and detection by means of gas chromatography coupled to mass spectrometry (GC-MS). The first method is optimised for the detection of the complete volatile profile in Citrus fruit, including those compounds derived from carotenoids. The second is a shorter method focused to the detection of volatile apocarotenoids in the tomato fruit.
    MeSH term(s) Carotenoids/analysis ; Carotenoids/chemistry ; Gas Chromatography-Mass Spectrometry ; Molecular Structure ; Plants/chemistry ; Volatile Organic Compounds/analysis ; Volatile Organic Compounds/chemistry
    Chemical Substances Volatile Organic Compounds ; Carotenoids (36-88-4) ; apocarotenal (V22N3E2U32)
    Language English
    Publishing date 2019-11-19
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9952-1_12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum)

    Liu, Ying / Andersson, Mariette / Granell, Antonio / Cardi, Teodoro / Hofvander, Per / Nicolia, Alessandro

    Plant cell reports. 2022 Sept., v. 41, no. 9

    2022  

    Abstract: KEY MESSAGE: We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with a high mutation rate. The application of genome ... ...

    Abstract KEY MESSAGE: We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with a high mutation rate. The application of genome editing as a research and breeding method has provided many possibilities to improve traits in many crops in recent years. In cultivated tomato (Solanum lycopersicum), so far only stable Agrobacterium-mediated transformation carrying CRISPR/Cas9 reagents has been established. Shoot regeneration from transfected protoplasts is the major bottleneck in the application of DNA-free genome editing via ribonucleoprotein-based CRISPR/Cas9 method in cultivated tomato. In this study, we report the implementation of a transgene-free breeding method for cultivated tomato by CRISPR/Cas9 technology, including the optimization of protoplast isolation and overcoming the obstacle in shoot regeneration from transfected protoplasts. We have identified that the shoot regeneration medium containing 0.1 mg/L IAA and 0.75 mg/L zeatin was the best hormone combination with a regeneration rate of up to 21.3%. We have successfully obtained regenerated plants with a high mutation rate four months after protoplast isolation and transfection. Out of 110 regenerated M₀ plants obtained, 35 (31.8%) were mutated targeting both SP and SP5G genes simultaneously and the editing efficiency was up to 60% in at least one allele in either SP or SP5G genes.
    Keywords CRISPR-Cas systems ; Solanum lycopersicum ; alleles ; mutants ; mutation rate ; protoplasts ; tomatoes ; transfection ; zeatin
    Language English
    Dates of publication 2022-09
    Size p. 1843-1852.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 8397-5
    ISSN 1432-203X ; 0721-085X ; 0721-7714
    ISSN (online) 1432-203X
    ISSN 0721-085X ; 0721-7714
    DOI 10.1007/s00299-022-02893-8
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Grafting improves tolerance to combined drought and heat stresses by modifying metabolism in citrus scion

    Balfagón, Damián / Rambla, José L. / Granell, Antonio / Arbona, Vicent / Gómez-Cadenas, Aurelio

    Environmental and experimental botany. 2022 Mar., v. 195

    2022  

    Abstract: Mediterranean basin and other citrus-growing areas, such as Florida or California, are among the most vulnerable regions to the impacts of global warming. Therefore, citrus will be likely subjected to increasing periods of water scarcity combined with ... ...

    Abstract Mediterranean basin and other citrus-growing areas, such as Florida or California, are among the most vulnerable regions to the impacts of global warming. Therefore, citrus will be likely subjected to increasing periods of water scarcity combined with high temperatures that will impair plant growth, development and yield. In citrus industry, grafting is used to improve varietal characteristics, such as abiotic stress tolerance. Previous research showed that under drought and heat stress conditions, Carrizo citrange (Poncirus trifoliata × Citrus sinensis) is a better rootstock than Cleopatra mandarin (Citrus reshni) because it induces a higher antioxidant activity on the scion, reducing oxidative damage and increasing plant tolerance. Here, it is shown that metabolic reconfiguration, including changes in carbohydrate and amino acid fluxes, are key responses for plant acclimation to stress conditions. Moreover, the importance of the rootstock on scion metabolic and hormonal responses to drought and heat stress combination has been addressed by using reciprocal grafting between Carrizo and Cleopatra genotypes. Thus, Carrizo as a rootstock improves the metabolic and hormonal response of Cleopatra scions to the stress combination by inducing the accumulation of protective compounds such as raffinose, galactinol and salicylic acid. In turn, Cleopatra as a rootstock reduces levels of raffinose, galactinol, proline, phenylalanine and tryptophan in Carrizo scions, which impairs plant tolerance to the stress combination. Our findings show the effect of the rootstock on scion metabolic response to stress combination and remark the importance of the rootstock in citrus plants exposed to harsh environmental conditions.
    Keywords Citroncirus webberi ; Citrus aurantium var. chrysocarpa ; Citrus sinensis ; Poncirus trifoliata ; acclimation ; antioxidant activity ; biochemical pathways ; drought ; heat ; heat stress ; industry ; mandarins ; metabolism ; phenylalanine ; plant growth ; proline ; raffinose ; rootstocks ; salicylic acid ; scions ; stress response ; stress tolerance ; tryptophan ; water shortages ; California ; Florida ; Mediterranean region
    Language English
    Dates of publication 2022-03
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 195968-2
    ISSN 0098-8472
    ISSN 0098-8472
    DOI 10.1016/j.envexpbot.2022.104793
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Constitutive expression of VviNAC17 transcription factor significantly induces the synthesis of flavonoids and other phenolics in transgenic grape berry cells.

    Badim, Hélder / Vale, Mariana / Coelho, Marco / Granell, Antonio / Gerós, Hernâni / Conde, Artur

    Frontiers in plant science

    2022  Volume 13, Page(s) 964621

    Abstract: VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape ...

    Abstract VviNAC17 is a grapevine transcription factor activated by ABA. Because ABA has been proposed as the main signal modulating the secondary metabolism in grape berry skins, here we postulated VviNAC17 as a positive regulator of secondary metabolism in grape cells. To validate the hypothesis,
    Language English
    Publishing date 2022-07-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2022.964621
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum).

    Liu, Ying / Andersson, Mariette / Granell, Antonio / Cardi, Teodoro / Hofvander, Per / Nicolia, Alessandro

    Plant cell reports

    2022  Volume 41, Issue 9, Page(s) 1843–1852

    Abstract: Key message: We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with a high mutation rate. The application of genome ... ...

    Abstract Key message: We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with a high mutation rate. The application of genome editing as a research and breeding method has provided many possibilities to improve traits in many crops in recent years. In cultivated tomato (Solanum lycopersicum), so far only stable Agrobacterium-mediated transformation carrying CRISPR/Cas9 reagents has been established. Shoot regeneration from transfected protoplasts is the major bottleneck in the application of DNA-free genome editing via ribonucleoprotein-based CRISPR/Cas9 method in cultivated tomato. In this study, we report the implementation of a transgene-free breeding method for cultivated tomato by CRISPR/Cas9 technology, including the optimization of protoplast isolation and overcoming the obstacle in shoot regeneration from transfected protoplasts. We have identified that the shoot regeneration medium containing 0.1 mg/L IAA and 0.75 mg/L zeatin was the best hormone combination with a regeneration rate of up to 21.3%. We have successfully obtained regenerated plants with a high mutation rate four months after protoplast isolation and transfection. Out of 110 regenerated M
    MeSH term(s) CRISPR-Cas Systems/genetics ; Gene Editing/methods ; Lycopersicon esculentum/genetics ; Plant Breeding ; Protoplasts ; Ribonucleoproteins/genetics
    Chemical Substances Ribonucleoproteins
    Language English
    Publishing date 2022-06-30
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 8397-5
    ISSN 1432-203X ; 0721-085X ; 0721-7714
    ISSN (online) 1432-203X
    ISSN 0721-085X ; 0721-7714
    DOI 10.1007/s00299-022-02893-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Pigment-Related Mutations Greatly Affect Berry Metabolome in San Marzano Tomatoes

    Dono, Gabriella / Rambla, José Luis / Frusciante, Sarah / Fabene, Eleonora / Gómez-Cadenas, Aurelio / Granell, Antonio / Diretto, Gianfranco / Mazzucato, Andrea

    Horticulturae. 2022 Jan. 28, v. 8, no. 2

    2022  

    Abstract: The study describes the alterations in metabolomic profiles of four tomato fruit mutations introgressed into Solanum lycopersicum cv. San Marzano, a well-known Italian traditional variety. Three lines carrying variants affecting the content of all ... ...

    Abstract The study describes the alterations in metabolomic profiles of four tomato fruit mutations introgressed into Solanum lycopersicum cv. San Marzano, a well-known Italian traditional variety. Three lines carrying variants affecting the content of all pigments, high pigment-1 (hp-1), hp-2, pigment diluter (pd), and a combination of Anthocyanin fruit and atroviolaceum (Aft_atv), were selected, and characterized. Biochemical analysis of 44 non-polar, 133 polar, and 65 volatile metabolites in ripe fruits revealed a wide range of differences between the variant lines and the recurrent parent San Marzano. Among non-polar compounds, many carotenoids, plastoquinones, and tocopherols increased in the fruit of high pigment lines, as well as in Aft_atv, whose β-carotene levels increased too. Interestingly, pd displayed enriched levels of xanthophylls (all-trans-neoxanthin and luteoxanthin) but, simultaneously, decreased levels of α-and β-/γ-tocopherols. Looking at the metabolites in the polar fraction, a significant decrease in sugar profile was observed in hp-1, pd, and Aft_atv. Conversely, many vitamins and organic acids increased in the hp-2 and Aft_atv lines, respectively. Overall, phenylpropanoids was the metabolic group with the highest extent of polar changes, with considerable increases of many compounds mainly in the case of Aft_atv, followed by the pd and hp-2 lines. Finally, several flavor-related compounds were found to be modified in all mutants, mostly due to increased levels in many benzenoid, lipid, and phenylalanine derivative volatiles, which are associated with sweeter taste and better aroma. Construction of metabolic maps, interaction networks, and correlation matrices gave an integrated representation of the large effect of single variants on the tomato fruit metabolome. In conclusion, the identified differences in the mutated lines might contribute to generating novel phenotypes in the traditional San Marzano type, with increased desirable nutraceutical and organoleptic properties.
    Keywords Solanum lycopersicum ; anthocyanins ; dietary supplements ; fruits ; landraces ; lipids ; metabolites ; metabolome ; metabolomics ; odors ; phenylalanine ; phenylpropanoids ; plastoquinones ; sugars ; taste ; tocopherols ; tomatoes ; xanthophylls
    Language English
    Dates of publication 2022-0128
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2813983-5
    ISSN 2311-7524
    ISSN 2311-7524
    DOI 10.3390/horticulturae8020120
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: A xanthophyll-derived apocarotenoid regulates carotenogenesis in tomato chromoplasts

    D’Ambrosio, Caterina / Stigliani, Adriana Lucia / Rambla, José L. / Frusciante, Sarah / Diretto, Gianfranco / Enfissi, Eugenia M.A. / Granell, Antonio / Fraser, Paul D. / Giorio, Giovanni

    Elsevier B.V. Plant Science. 2023 Mar., v. 328 p.111575-

    2023  

    Abstract: Carotenoids possess important biological functions that make them essential components of the human diet. β-Carotene and some other carotenoids have vitamin A activity while lutein and zeaxanthin, typically referred to as the macular pigments, are ... ...

    Abstract Carotenoids possess important biological functions that make them essential components of the human diet. β-Carotene and some other carotenoids have vitamin A activity while lutein and zeaxanthin, typically referred to as the macular pigments, are involved in good vision and in delaying the onset of age-related eye diseases. In order to create a zeaxanthin-producing tomato fruit, two transgenic lines, one with a high β-carotene cyclase activity and the other with a high β-carotene hydroxylase activity, have been genetically crossed. Ripe fruits from the resulting progeny contained significant levels of violaxanthin, antheraxanthin, and xanthophyll esters. However, their zeaxanthin content was not as high as expected, and the total level of carotenoids was only 25% of the carotenoids found in ripe fruits of the comparator line. Targeted transcript analysis and apocarotenoids determinations indicated that transcriptional regulation of the pathway or degradation of synthesized carotenoids were not responsible for the low carotenoid content of hybrid fruits which instead appeared to result from a substantial reduction of carotenoid biosynthesis. Notably, the content of an unidentified hydroxylated cyclic (C13) apocarotenoid was 13 times higher in the hybrid fruits than in the control fruits. Furthermore, a GC-MS-based metabolite profiling demonstrated a perturbation of carotenogenesis in ripening hybrid fruits compatible with a block of the pathway. Moreover, carotenoid profiling on leaf, fruit, and petal samples from a set of experimental lines carrying the hp3 mutation, in combination with the two transgenes, indicated that the carotenoid biosynthesis in petal and fruit chromoplasts could be regulated. Altogether the data were consistent with the hypothesis of the regulation of the carotenoid pathway in tomato chromoplasts through a mechanism of feedback inhibition mediated by a xanthophyll-derived apocarotenoid. This chromoplast-specific post-transcriptional mechanism was disclosed in transgenic fruits of HU hybrid owing to the abnormal production of zeaxanthin and antheraxanthin, the more probable precursors of the apocarotenoid signal. A model describing the regulation of carotenoid pathway in tomato chromoplasts is presented.
    Keywords antheraxanthin ; biosynthesis ; chromoplasts ; eyes ; fruits ; genetically modified organisms ; human nutrition ; hybrids ; hydroxylation ; leaves ; lutein ; metabolites ; models ; mutation ; progeny ; tomatoes ; transcription (genetics) ; transgenes ; violaxanthin ; vision ; vitamin A ; zeaxanthin ; Fruit ripening ; Carotenoid metabolism regulation ; Lcyb ; CrtRb2 ; Transgene over-expression ; hp3 mutation
    Language English
    Dates of publication 2023-03
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 742010-9
    ISSN 1873-2259 ; 0168-9452
    ISSN (online) 1873-2259
    ISSN 0168-9452
    DOI 10.1016/j.plantsci.2022.111575
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Genetic and molecular mechanisms underlying the

    Picarella, Maurizio E / Ruiu, Fabrizio / Selleri, Luigi / Presa, Silvia / Mizzotti, Chiara / Masiero, Simona / Colombo, Lucia / Soressi, Gian Piero / Granell, Antonio / Mazzucato, Andrea

    Frontiers in plant science

    2024  Volume 15, Page(s) 1329949

    Abstract: Parthenocarpy allows fruit set independently of fertilization. In parthenocarpic-prone tomato genotypes, fruit set can be achieved under pollen-limiting environmental conditions and in sterile mutants. Parthenocarpy is also regarded as a quality-related ... ...

    Abstract Parthenocarpy allows fruit set independently of fertilization. In parthenocarpic-prone tomato genotypes, fruit set can be achieved under pollen-limiting environmental conditions and in sterile mutants. Parthenocarpy is also regarded as a quality-related trait, when seedlessness is associated with positive fruit quality aspects. Among the different sources of genetic parthenocarpy described in tomato, the
    Language English
    Publishing date 2024-03-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2024.1329949
    Database MEDical Literature Analysis and Retrieval System OnLINE

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