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  1. Article: Comparison of methods for isolating fungal DNA.

    Galliano, Ilaria / Daprà, Valentina / Zaniol, Elena / Alliaudi, Carla / Graziano, Elisa / Montanari, Paola / Calvi, Cristina / Bergallo, Massimiliano

    Practical laboratory medicine

    2021  Volume 25, Page(s) e00221

    Abstract: Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus : Design: Five DNA extraction procedures were carried out in a Class 2 Biosafety cabinet in a dedicated room with suitable biosafety precautions ... ...

    Abstract Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus
    Design: Five DNA extraction procedures were carried out in a Class 2 Biosafety cabinet in a dedicated room with suitable biosafety precautions and appropriate biowaste disposal methods. A total of 6
    Results: From the viewpoint of concentration and purity, methods A shown abundant amount of fungal DNA whereas methods E report a pure fungal DNA with R260/280 of 1.7 near the optimal 1.8. The DNA quantity reach statistically difference at ANOVA test with p value 0.0005.
    Conclusion: Overall, the E method was the most efficient method in the extraction of DNA from fungal cultures compared to the other methods considering time, cost, technical expertise, and instrumentation. Use of this assay will allow researchers to obtain DNA from fungi quickly for use in molecular assays.
    Language English
    Publishing date 2021-04-17
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2834973-8
    ISSN 2352-5517
    ISSN 2352-5517
    DOI 10.1016/j.plabm.2021.e00221
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Severe rosuvastatin accumulation with rhabdomyolysis due to drug interactions and low cardiac output syndrome.

    Previsdomini, Marco / Graziano, Elisa / Decosterd, Laurent / Courlet, Perrine / Perren, Andreas / Ceschi, Alessandro

    British journal of clinical pharmacology

    2019  Volume 85, Issue 7, Page(s) 1616–1618

    MeSH term(s) Aged, 80 and over ; Cardiac Output, Low/complications ; Drug Interactions ; Humans ; Hydroxymethylglutaryl-CoA Reductase Inhibitors/administration & dosage ; Hydroxymethylglutaryl-CoA Reductase Inhibitors/adverse effects ; Male ; Rhabdomyolysis/chemically induced ; Rosuvastatin Calcium/administration & dosage ; Rosuvastatin Calcium/adverse effects
    Chemical Substances Hydroxymethylglutaryl-CoA Reductase Inhibitors ; Rosuvastatin Calcium (83MVU38M7Q)
    Language English
    Publishing date 2019-05-03
    Publishing country England
    Document type Case Reports ; Letter
    ZDB-ID 188974-6
    ISSN 1365-2125 ; 0306-5251 ; 0264-3774
    ISSN (online) 1365-2125
    ISSN 0306-5251 ; 0264-3774
    DOI 10.1111/bcp.13950
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Modulation of human endogenous retroviruses -H, -W and -K transcription by microbes.

    Bergallo, Massimiliano / Galliano, Ilaria / Montanari, Paola / Zaniol, Elena / Graziano, Elisa / Calvi, Cristina / Alliaudi, Carla / Daprà, Valentina / Savino, Francesco

    Microbes and infection

    2020  Volume 22, Issue 8, Page(s) 366–370

    Abstract: The human endogenous retroviruses (HERVs) are endogenous retroviruses that are inserted into the germ cell DNA of humans over 30 million years ago. Using real-time RT-PCR we describe HERV modulation by commensal microbes in the human gut. Infants, ... ...

    Abstract The human endogenous retroviruses (HERVs) are endogenous retroviruses that are inserted into the germ cell DNA of humans over 30 million years ago. Using real-time RT-PCR we describe HERV modulation by commensal microbes in the human gut. Infants, exclusively or predominant breast milk feeding, less than 12 weeks of age, during bacteria gut colonization, were assessed for eligibility. Our data demonstrate that the colonization with commensal microbes, in particular, Bifidobacterium spp., of the gut causes modulation of HERVs.
    Language English
    Publishing date 2020-02-06
    Publishing country France
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1465093-9
    ISSN 1769-714X ; 1286-4579
    ISSN (online) 1769-714X
    ISSN 1286-4579
    DOI 10.1016/j.micinf.2020.01.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Association Between HDL Cholesterol and QTc Interval: A Population-Based Epidemiological Study.

    Del Giorno, Rosaria / Gabutti, Sofia / Troiani, Chiara / Stefanelli, Kevyn / Falciano, Raffaele / Graziano, Elisa / Rochat Negro, Tommaso / Gabutti, Luca

    Journal of clinical medicine

    2019  Volume 8, Issue 10

    Abstract: Previous experimental studies showed that increasing high-density lipoprotein cholesterol (HDL) cholesterol shortens cardiac ventricular repolarization and the QT interval corrected for heart rate (QTc). However, little is known about the epidemiological ...

    Abstract Previous experimental studies showed that increasing high-density lipoprotein cholesterol (HDL) cholesterol shortens cardiac ventricular repolarization and the QT interval corrected for heart rate (QTc). However, little is known about the epidemiological relationship between HDL and QTc. The potential antiarrhythmic effect of HDL cholesterol remains a speculative hypothesis. In this cross-sectional population based study in adults living in the Italian-speaking part of Switzerland, we aimed to explore the association between HDL cholesterol and the QTc interval in the general population. A total of 1202 subjects were screened. electrocardiogram (ECG) recordings, measurements of lipid parameters and other laboratory tests were performed. QTc was corrected using Bazett's (QTc
    Language English
    Publishing date 2019-09-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm8101527
    Database MEDical Literature Analysis and Retrieval System OnLINE

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