Article: Comparison of methods for isolating fungal DNA.
2021 Volume 25, Page(s) e00221
Abstract: Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus : Design: Five DNA extraction procedures were carried out in a Class 2 Biosafety cabinet in a dedicated room with suitable biosafety precautions ... ...
Abstract | Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Design: Five DNA extraction procedures were carried out in a Class 2 Biosafety cabinet in a dedicated room with suitable biosafety precautions and appropriate biowaste disposal methods. A total of 6 Results: From the viewpoint of concentration and purity, methods A shown abundant amount of fungal DNA whereas methods E report a pure fungal DNA with R260/280 of 1.7 near the optimal 1.8. The DNA quantity reach statistically difference at ANOVA test with p value 0.0005. Conclusion: Overall, the E method was the most efficient method in the extraction of DNA from fungal cultures compared to the other methods considering time, cost, technical expertise, and instrumentation. Use of this assay will allow researchers to obtain DNA from fungi quickly for use in molecular assays. |
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Language | English |
Publishing date | 2021-04-17 |
Publishing country | Netherlands |
Document type | Journal Article |
ZDB-ID | 2834973-8 |
ISSN | 2352-5517 |
ISSN | 2352-5517 |
DOI | 10.1016/j.plabm.2021.e00221 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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