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  1. Article ; Online: Imaging of Subcellular Distribution of Platinum in Single Cells Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry.

    Managh, Amy J / Greenhalgh, Calum J

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2752, Page(s) 215–226

    Abstract: Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) is a well-established and sensitive analytical technique, which provides high-resolution imaging of endogenous elements, element tagged-markers, metal-containing nanoparticles, and ... ...

    Abstract Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) is a well-established and sensitive analytical technique, which provides high-resolution imaging of endogenous elements, element tagged-markers, metal-containing nanoparticles, and metallodrugs within cells. Here we describe a protocol for imaging the subcellular distribution of platinum within A549 cells, following their incubation with the platinum-based anticancer agent, Oxaliplatin. We outline the essential steps in sample preparation and instrumental setup and discuss how the current generation of low-dispersion instruments facilitates new approaches to data acquisition and image processing. The protocol described herein can be easily adapted for other cell lines and metal-containing labeling agents.
    MeSH term(s) Diagnostic Imaging ; Spectrum Analysis ; Image Processing, Computer-Assisted ; Laser Therapy ; Platinum ; Mass Spectrometry
    Chemical Substances Platinum (49DFR088MY)
    Language English
    Publishing date 2024-01-09
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3621-3_14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Laser ablation inductively coupled plasma mass spectrometry as a novel clinical imaging tool to detect asbestos fibres in malignant mesothelioma.

    Voloaca, Oana M / Greenhalgh, Calum J / Cole, Laura M / Clench, Malcolm R / Managh, Amy J / Haywood-Small, Sarah L

    Rapid communications in mass spectrometry : RCM

    2020  Volume 34, Issue 21, Page(s) e8906

    Abstract: Rationale: Malignant pleural mesothelioma is an extremely aggressive and incurable malignancy associated with prior exposure to asbestos fibres. Difficulties remain in relation to early diagnosis, notably due to impeded identification of asbestos in ... ...

    Abstract Rationale: Malignant pleural mesothelioma is an extremely aggressive and incurable malignancy associated with prior exposure to asbestos fibres. Difficulties remain in relation to early diagnosis, notably due to impeded identification of asbestos in lung tissue. This study describes a novel laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) imaging approach to identify asbestos within mesothelioma models with clinical significance.
    Methods: Human mesothelioma cells were exposed to different types of asbestos fibres and prepared on plastic slides for LA-ICP-MS analysis. No further sample preparation was required prior to analysis, which was performed using an NWR Image 266 nm laser ablation system coupled to an Element XR sector-field ICP mass spectrometer, with a lateral resolution of 2 μm. Data was processed using LA-ICP-MS ImageTool v1.7 with the final graphic production made using DPlot software.
    Results: Four different mineral fibres were successfully identified within the mesothelioma samples based on some of the most abundant elements that make up these fibres (Si, Mg and Fe). Using LA-ICP-MS as an imaging tool provided information on the spatial distribution of the fibres at cellular level, which is essential in asbestos detection within tissue samples. Based on the metal counts generated by the different types of asbestos, different fibres can be identified based on shape, size, and elemental composition. Detection of Ca was attempted but requires further optimisation.
    Conclusions: Detection of asbestos fibres in lung tissues is very useful, if not necessary, to complete the pathological dt9iagnosis of asbestos-related malignancies in the medicolegal field. For the first time, this study demonstrates the successful application of LA-ICP-MS imaging to identify asbestos fibres and other mineral fibres within mesothelioma samples. Ultimately, high-resolution, fast-speed LA-ICP-MS analysis has the potential to be integrated into clinical workflow to aid earlier detection and stratification of mesothelioma patient samples.
    MeSH term(s) Asbestos/analysis ; Asbestos/chemistry ; Cell Line, Tumor ; Humans ; Lasers ; Lung Neoplasms/diagnostic imaging ; Lung Neoplasms/pathology ; Mass Spectrometry/methods ; Mesothelioma, Malignant/diagnostic imaging ; Mesothelioma, Malignant/pathology ; Microscopy/methods
    Chemical Substances Asbestos (1332-21-4)
    Language English
    Publishing date 2020-07-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 58731-x
    ISSN 1097-0231 ; 0951-4198
    ISSN (online) 1097-0231
    ISSN 0951-4198
    DOI 10.1002/rcm.8906
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3461: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Exploration of Matrix Effects in Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Cisplatin-Treated Tumors.

    Greenhalgh, Calum J / Karekla, Ellie / Miles, Gareth J / Powley, Ian R / Costa, Catia / de Jesus, Janella / Bailey, Melanie J / Pritchard, Catrin / MacFarlane, Marion / Pringle, J Howard / Managh, Amy J

    Analytical chemistry

    2020  Volume 92, Issue 14, Page(s) 9847–9855

    Abstract: The use of a low aerosol dispersion ablation chamber within a laser ablation inductively coupled plasma mass spectrometer (LA-ICP-MS) setup allows for high-resolution, high-speed imaging of the distribution of elements within a sample. Here we show how ... ...

    Abstract The use of a low aerosol dispersion ablation chamber within a laser ablation inductively coupled plasma mass spectrometer (LA-ICP-MS) setup allows for high-resolution, high-speed imaging of the distribution of elements within a sample. Here we show how this enhanced capability creates new analytical problems and solutions. We report the distribution of platinum at the cellular level in non-small cell lung cancer (NSCLC) explant models after treatment with clinically relevant doses of cisplatin. This revealed for the first time a correlation between the platinum signal and the presence of carbon deposits within lung tissue. We show how complementary ion beam analysis techniques, particle-induced X-ray emission (PIXE) and elastic backscattering spectrometry (EBS), can be used to explore potential matrix effects in LA-ICP-MS data. For these samples, it was confirmed that the enhancement was unlikely to have resulted from a matrix effect alone. Thus, the presence of carbon deposits within tissue has potential implications for the effective distribution of the cisplatin drug.
    MeSH term(s) Antineoplastic Agents/therapeutic use ; Carbon/chemistry ; Carcinoma, Non-Small-Cell Lung ; Cisplatin/therapeutic use ; Humans ; Laser Therapy ; Lung Neoplasms/chemistry ; Lung Neoplasms/drug therapy ; Mass Spectrometry/methods ; Spheroids, Cellular ; Tissue Culture Techniques
    Chemical Substances Antineoplastic Agents ; Carbon (7440-44-0) ; Cisplatin (Q20Q21Q62J)
    Language English
    Publishing date 2020-07-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.0c01347
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

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  4. Article: Exploration of Matrix Effects in Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Cisplatin-Treated Tumors

    Greenhalgh, Calum J / Karekla, Ellie / Miles, Gareth J / Powley, Ian R / Costa, Catia / de Jesus, Janella / Bailey, Melanie J / Pritchard, Catrin / MacFarlane, Marion / Pringle, J. Howard / Managh, Amy J

    Analytical chemistry. 2020 June 17, v. 92, no. 14

    2020  

    Abstract: The use of a low aerosol dispersion ablation chamber within a laser ablation inductively coupled plasma mass spectrometer (LA-ICP–MS) setup allows for high-resolution, high-speed imaging of the distribution of elements within a sample. Here we show how ... ...

    Abstract The use of a low aerosol dispersion ablation chamber within a laser ablation inductively coupled plasma mass spectrometer (LA-ICP–MS) setup allows for high-resolution, high-speed imaging of the distribution of elements within a sample. Here we show how this enhanced capability creates new analytical problems and solutions. We report the distribution of platinum at the cellular level in non-small cell lung cancer (NSCLC) explant models after treatment with clinically relevant doses of cisplatin. This revealed for the first time a correlation between the platinum signal and the presence of carbon deposits within lung tissue. We show how complementary ion beam analysis techniques, particle-induced X-ray emission (PIXE) and elastic backscattering spectrometry (EBS), can be used to explore potential matrix effects in LA-ICP–MS data. For these samples, it was confirmed that the enhancement was unlikely to have resulted from a matrix effect alone. Thus, the presence of carbon deposits within tissue has potential implications for the effective distribution of the cisplatin drug.
    Keywords X-radiation ; aerosols ; analytical chemistry ; atomic absorption spectrometry ; carbon ; cisplatin ; lung neoplasms ; lungs ; platinum ; spectrometers
    Language English
    Dates of publication 2020-0617
    Size p. 9847-9855.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.0c01347
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4' 52/94: Show issues
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