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  1. Article: Production of heterologous proteins using the baculovirus/insect expression system.

    Griffiths, C M / Page, M J

    Methods in molecular biology (Clifton, N.J.)

    1997  Volume 75, Page(s) 427–440

    MeSH term(s) Animals ; Baculoviridae ; Cloning, Molecular/methods ; Humans ; Insecta ; Protein Biosynthesis ; Proteins/genetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics
    Chemical Substances Proteins ; Recombinant Proteins
    Language English
    Publishing date 1997
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1385/0-89603-441-0:427
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  2. Article: The baculovirus P10 protein of Autographa californica nucleopolyhedrovirus forms two distinct cytoskeletal-like structures and associates with polyhedral occlusion bodies during infection

    Carpentier, D.C.J / Griffiths, C.M / King, L.A

    Virology. 2008 Feb. 20, v. 371, issue 2

    2008  

    Keywords Autographa californica multiple nucleopolyhedrovirus ; cytoskeleton ; viral proteins
    Language English
    Dates of publication 2008-0220
    Size p. 278-291.
    Document type Article
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/j.virol.2007.09.043
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    Z 3686: Show issues

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  3. Article: Reconstruction of the glottis after total laryngectomy.

    Griffiths, C M

    Ear, nose, & throat journal

    1981  Volume 60, Issue 6, Page(s) 259–264

    MeSH term(s) Glottis/surgery ; Humans ; Laryngectomy ; Methods ; Tracheotomy
    Language English
    Publishing date 1981-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 750153-5
    ISSN 1942-7522 ; 0145-5613
    ISSN (online) 1942-7522
    ISSN 0145-5613
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  4. Article: Neoglottic reconstruction after total laryngectomy. Staffieri's techniques: a report of 18 cases.

    Griffiths, C M

    Archives of otolaryngology (Chicago, Ill. : 1960)

    1980  Volume 106, Issue 2, Page(s) 77–79

    Abstract: Mario Staffieri and associates developed a technique for reconstruction of a phonatory neoglottis in 1970. Since then Staffiere has performed over 100 of these procedures with approximately a 90% success rate. The technique has been used in 18 cases at ... ...

    Abstract Mario Staffieri and associates developed a technique for reconstruction of a phonatory neoglottis in 1970. Since then Staffiere has performed over 100 of these procedures with approximately a 90% success rate. The technique has been used in 18 cases at The University of Texas Medical Branch in Galveston with certain variations. All patients received a wide field total laryngectomy for T3 or T4 endolaryngeal or pyriform sinus carcinomas. Of these 18 patients, two did not have neoglotic fistulas created. Of the 16 patients who remained in the series, 12 have a good voice. One died of carcinoma of the lung, one had spontaneous closure after postoperative radiotherapy, one aspirated severely and was closed surgically, and the last patient had a postoperative wound infection and closed spontaneously. He developed good esophageal speech.
    MeSH term(s) Glottis/surgery ; Humans ; Laryngectomy/methods ; Speech, Alaryngeal/methods ; Surgical Flaps
    Language English
    Publishing date 1980-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 211582-7
    ISSN 0003-9977 ; 0096-6894 ; 0276-0673
    ISSN 0003-9977 ; 0096-6894 ; 0276-0673
    DOI 10.1001/archotol.1980.00790260009004
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  5. Article: Multigrain seabed sediment transport modelling for the south-west Australian Shelf

    Li, F / Griffiths, C.M / Dyt, C.P / Weill, P / Feng, M / Salles, T / Jenkins, C

    Marine & freshwater research. 2009, v. 60, no. 7

    2009  

    Abstract: With increasing concerns about climate change and sea-level rise, there is a need for a comprehensive understanding of the sedimentary processes involved in the erosion, transport and deposition of sediment on the continental shelf. In the present paper, ...

    Abstract With increasing concerns about climate change and sea-level rise, there is a need for a comprehensive understanding of the sedimentary processes involved in the erosion, transport and deposition of sediment on the continental shelf. In the present paper, long-term and large-scale seabed morphological changes on the south-west Australian continental shelf were investigated by a comprehensive sediment transport model, Sedsim. The investigated area covers the continental shelf and abyssal basins of the south-western region. The regional seabed is sensitive to environmental forces and sediment supply, and most terrigenous sediment carried down by major rivers is trapped in inland lakes or estuaries. Only a small fraction of fine-grain sediment reaches the continental shelf. The simulation has also confirmed that the Leeuwin Current and high-energy waves play the most important roles in regional long-term seabed evolution. Although the numerical implementation only approximates some forcing and responses, it represents a significant step forward in understanding the nature of potential long-term seabed change as a response to possible climate change scenarios. The 50-year forecast on the seabed morphological changes provides a reference for the management of coastal and offshore resources, as well as infrastructure, in a sustainable way.
    Keywords climate change
    Language English
    Size p. 774-785.
    Publishing place Collingwood, Victoria: CSIRO Publishing
    Document type Article
    ZDB-ID 1283028-8
    ISSN 1323-1650
    ISSN 1323-1650
    DOI 10.1071/MF08049
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    Z 4948: Show issues

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  6. Article ; Online: Describing the burden of the COVID-19 pandemic in people with psoriasis: findings from a global cross-sectional study.

    Mahil, S K / Yates, M / Yiu, Z Z N / Langan, S M / Tsakok, T / Dand, N / Mason, K J / McAteer, H / Meynell, F / Coker, B / Vincent, A / Urmston, D / Vesty, A / Kelly, J / Lancelot, C / Moorhead, L / Bachelez, H / Capon, F / Contreras, C R /
    De La Cruz, C / Di Meglio, P / Gisondi, P / Jullien, D / Lambert, J / Naldi, L / Norton, S / Puig, L / Spuls, P / Torres, T / Warren, R B / Waweru, H / Weinman, J / Brown, M A / Galloway, J B / Griffiths, C M / Barker, J N / Smith, C H

    Journal of the European Academy of Dermatology and Venereology : JEADV

    2021  Volume 35, Issue 10, Page(s) e636–e640

    MeSH term(s) COVID-19 ; Cross-Sectional Studies ; Humans ; Pandemics ; Psoriasis/epidemiology ; SARS-CoV-2
    Language English
    Publishing date 2021-08-19
    Publishing country England
    Document type Letter
    ZDB-ID 1128828-0
    ISSN 1468-3083 ; 0926-9959
    ISSN (online) 1468-3083
    ISSN 0926-9959
    DOI 10.1111/jdv.17450
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    Zs.A 3492: Show issues Location:
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    Zs.MO 413: Show issues

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  7. Article: Vertical transmission of sublethal granulovirus infection in the Indian meal moth, Plodia interpunctella

    Burden, J.P / Griffiths, C.M / Cory, J.S / Smith, P / Sait, S.M

    Molecular ecology. Mar 2002. v. 11 (3)

    2002  

    Keywords infection ; Plodia interpunctella ; Granulovirus ; messenger RNA ; viral proteins ; ovaries ; testes ; DNA replication ; developmental stages
    Language English
    Dates of publication 2002-03
    Size p. 547-555.
    Document type Article
    ZDB-ID 1126687-9
    ISSN 1365-294X ; 0962-1083
    ISSN (online) 1365-294X
    ISSN 0962-1083
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    Z 2005/723: Show issues
    Nees-Institut: Meckenheimer Allee: Show issues

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  8. Article: Vertical transmission of sublethal granulovirus infection in the Indian meal moth, Plodia interpunctella.

    Burden, J P / Griffiths, C M / Cory, J S / Smith, P / Sait, S M

    Molecular ecology

    2002  Volume 11, Issue 3, Page(s) 547–555

    Abstract: Knowledge of the mechanisms of pathogen persistence in relation to fluctuations in host density is crucial to our understanding of disease dynamics. In the case of insect baculoviruses, which are typically transmitted horizontally via a lifestage that ... ...

    Abstract Knowledge of the mechanisms of pathogen persistence in relation to fluctuations in host density is crucial to our understanding of disease dynamics. In the case of insect baculoviruses, which are typically transmitted horizontally via a lifestage that can persist outside the host, a key issue that remains to be elucidated is whether the virus can also be transmitted vertically as a sublethal infection. We show that RNA transcripts for the Plodia interpunctella GV granulin gene are present in a high proportion of P. interpunctella insects that survive virus challenge. Granulin is a late-expressed gene that is only transcribed after viral genome replication, its presence thus strongly indicates that viral genome replication has occurred. Almost all insects surviving the virus challenge tested positive for viral RNA in the larval and pupal stage. However, this proportion declined in the emerging adults. Granulin mRNA was also detected in both the ovaries and testes, which may represent a putative mechanism by which reduced fecundity in sublethally affected hosts might be manifested. RNA transcripts were also detected in 60-80% of second-generation larvae that were derived from mating surviving adults, but there was no difference between the sexes, with both males and females capable of transmitting a sublethal infection to their offspring. The data indicate that low-level persistent infection, with at least limited gene expression, can occur in P. interpunctella following survival of a granulovirus challenge. We believe that this is the first demonstration of a persistent, sublethal infection by a baculovirus to be initiated by a sublethal virus dose. We hypothesize that the 'latent' baculovirus infections frequently referred to in the literature may also be low level persistent, sublethal infections resulting from survival from initial baculovirus exposure.
    MeSH term(s) Animals ; Female ; Genes, Viral ; Genome, Viral ; Granulovirus/genetics ; Granulovirus/pathogenicity ; Granulovirus/physiology ; Male ; Moths/virology ; Occlusion Body Matrix Proteins ; Polymerase Chain Reaction ; Viral Proteins/genetics ; Viral Structural Proteins ; Virus Replication
    Chemical Substances Occlusion Body Matrix Proteins ; Viral Proteins ; Viral Structural Proteins ; granulin protein, granulovirus
    Language English
    Publishing date 2002-01-28
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1126687-9
    ISSN 1365-294X ; 0962-1083
    ISSN (online) 1365-294X
    ISSN 0962-1083
    DOI 10.1046/j.0962-1083.2001.01439.x
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  9. Article: Anchored simple-sequence repeats as primers to generate species-specific DNA markers in Lolium and Festuca grasses

    Pasakinskiene, I / Griffiths, C.M / Bettany, A.J.E / Paplauskiene, V / Humphreys, M.W

    Theoretical and applied genetics. Feb 2000. v. 100 (3/4)

    2000  

    Abstract: Simple-sequence repeats (SSRS) comprising three tetranucleotide repeat sequences with two-base 'anchors', namely 5'-(AGAC)4GC, 5'-AC(GACA)4 and 5'-(GACA)4GT, were used in PCR reactions as primers to develop inter-SSR DNA fingerprints of the outbreeding ... ...

    Abstract Simple-sequence repeats (SSRS) comprising three tetranucleotide repeat sequences with two-base 'anchors', namely 5'-(AGAC)4GC, 5'-AC(GACA)4 and 5'-(GACA)4GT, were used in PCR reactions as primers to develop inter-SSR DNA fingerprints of the outbreeding grass species Lolium multiflorum, L. perenne, Festuca pratensis and F. arundinacea. Each species was represented by DNA samples from 3 to 6 varieties. In all four species distinctive species-specific DNA profiles were produced that were common across a number of varieties despite their diverse origin. While the fingerprints of the two ryegrasses, L. multiflorum and L. perenne, were the most similar, a number of inter-SSR DNA markers were generated that enabled them to be distinguished from each other. Some slight variations were found between varieties, which provided putative variety-specific markers for cultivar identification. In addition, variations in the DNA profiles of the genotypes of L. multiflorum and F. pratensis were examined, and the results showed that variety-specific fingerprints are integrated patterns made up from the profiles of individual genotypes. Amongst the primers used, AC(GACA)4 generated the best distinction between Lolium and Festuca individuals and provides an effective new tool for genome identification. A number of species-discriminating sequences, ranging in size between 550 bp and 1,600 bp, were cloned: three clones for F. pratensis, one clone for L. multiflorum and one clone for F. arundinacea. A F. pratensis fragment pFp 78H582 was sequenced. Southern hybridization confirmed the presence of this fragment in F. arundinacea (which contains one genome of F. pratensis), but no homology was found with L. multiflorum. However, a F. arundinacea clone amplified with (GACA)4GT, pFa 104H1350, was found to be unique to the F. arundinacea genome.
    Keywords Lolium perenne ; Lolium multiflorum ; Festuca pratensis ; Festuca arundinacea ; genetic markers ; polymerase chain reaction ; DNA ; DNA fingerprinting ; varieties ; Southern blotting ; nucleotide sequences ; repetitive sequences ; genotype ; DNA primers
    Language English
    Dates of publication 2000-02
    Size p. 384-390.
    Document type Article
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
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    Z 584: Show issues

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  10. Article: Sequence of the nucleocapsid protein gene of Machupo virus: close relationship with another South American pathogenic arenavirus, Junín.

    Griffiths, C M / Wilson, S M / Clegg, J C

    Archives of virology

    1992  Volume 124, Issue 3-4, Page(s) 371–377

    Abstract: The sequence of the nucleocapsid (N) protein of Machupo virus (causative agent of Bolivian haemorrhagic fever) has been determined, and used to infer a phylogenetic relationship to other arenaviruses. The relationship of the virus to Junín and Tacaribe ... ...

    Abstract The sequence of the nucleocapsid (N) protein of Machupo virus (causative agent of Bolivian haemorrhagic fever) has been determined, and used to infer a phylogenetic relationship to other arenaviruses. The relationship of the virus to Junín and Tacaribe viruses, together with previous demonstrations of antigenic similarity and cross-protection by heterologous viruses, suggest that vaccines developed against Argentine haemorrhagic fever might also be effective against the Bolivian disease.
    MeSH term(s) Amino Acid Sequence ; Arenaviruses, New World/classification ; Arenaviruses, New World/genetics ; Base Sequence ; Capsid/genetics ; DNA, Viral ; Molecular Sequence Data ; Phylogeny ; RNA, Viral ; Viral Core Proteins/genetics
    Chemical Substances DNA, Viral ; RNA, Viral ; Viral Core Proteins
    Language English
    Publishing date 1992
    Publishing country Austria
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/bf01309817
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