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  1. Article: Hypercalcemia, an Important Puzzle Piece in Uncommon Onset Pediatric Sarcoidosis-A Case Report and a Review of the Literature.

    Mărginean, Cristina Oana / Meliţ, Lorena Elena / Grigorescu, Gabriel / Puiac, Claudiu / Simu, Iunius

    Frontiers in pediatrics

    2020  Volume 8, Page(s) 497

    Abstract: Sarcoidosis (SD) is a systemic granulomatous condition that is especially encountered in young adults and rarely in children, affecting predominantly the lungs and lymph nodes. We report the case of a 14-year-old teenage boy admitted to our clinic for ... ...

    Abstract Sarcoidosis (SD) is a systemic granulomatous condition that is especially encountered in young adults and rarely in children, affecting predominantly the lungs and lymph nodes. We report the case of a 14-year-old teenage boy admitted to our clinic for nausea, vomiting, and weight loss. Clinical examination at the time of admission revealed malaise, pallor, and abdominal tenderness in the epigastric area at palpation. Laboratory tests revealed an elevated level of hemoglobin, mild thrombocytosis, increased erythrocyte sedimentation rate, and a mild increase in creatinine and urea levels along with hypercalcemia. An abdominal ultrasound revealed a right ectopic kidney, whereas the upper digestive endoscopy showed intense hyperemia and edema of the gastric mucosa. Thoracic computed tomography scan revealed giant hilar and mediastinal lymphadenopathy, along with multiple micronodules within the lung parenchyma and ground-glass aspect. The level of angiotensin-converting enzyme was high, parathormone was normal, and vitamin D level was low. Pathological examination of the bronchial, mediastinal, and lung biopsies established the diagnosis of SD. We administered oral corticosteroids for 2 months with outstandingly favorable outcome and no signs of recurrence 6 months after the cessation of the therapy.
    Language English
    Publishing date 2020-08-26
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2711999-3
    ISSN 2296-2360
    ISSN 2296-2360
    DOI 10.3389/fped.2020.00497
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Combinatorial approach for improving the outcome of angiogenic therapy in ischemic tissues.

    Odent Grigorescu, G / Preda, M B / Radu, E / Rosca, A-M / Tutuianu, R / Mitroi, D N / Simionescu, M / Burlacu, A

    Biomaterials

    2015  Volume 60, Page(s) 72–81

    Abstract: Two major populations of endothelial progenitor cells (EPC), namely endothelial colony forming cells (ECFC, or late outgrowth EPC) and circulating angiogenic cells (CAC, or early outgrowth EPC) have been reported to play important roles in vasculogenesis ...

    Abstract Two major populations of endothelial progenitor cells (EPC), namely endothelial colony forming cells (ECFC, or late outgrowth EPC) and circulating angiogenic cells (CAC, or early outgrowth EPC) have been reported to play important roles in vasculogenesis in numerous pathological conditions. However, the poor retention of cells into the ischemic tissue and neovessel fragility are two major flaws that need to be overcome for successful angiogenic therapy. The objective of this study was to explore and exploit the functional properties of EPC populations in order to increase the effectiveness of post-ischemic cell therapy. The results indicate different, still complementary, effects of the two EPC populations on adherence and proliferation of vascular endothelial cells. Matrigel plug assay and mouse hind limb ischemia model showed that concomitant administration of CAC-secreted factors and ECFC resulted in three-fold increase in local cell retention and improved muscle perfusion, vessel maturation and hind limb regeneration, in comparison to either treatment alone. By concluding, factors secreted by CAC co-administered at the time of ECFC transplantation improve tissue regeneration and vascular repair through stabilization of newly-derived blood vessels.
    MeSH term(s) Animals ; Cell Adhesion ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Collagen/chemistry ; Drug Combinations ; Endothelial Cells/cytology ; Endothelial Cells/transplantation ; Hindlimb/blood supply ; Humans ; Ischemia/therapy ; Laminin/chemistry ; Mice ; Neovascularization, Physiologic ; Proteoglycans/chemistry ; Stem Cell Transplantation ; Stem Cells/cytology ; Tissue Scaffolds/chemistry
    Chemical Substances Drug Combinations ; Laminin ; Proteoglycans ; matrigel (119978-18-6) ; Collagen (9007-34-5)
    Language English
    Publishing date 2015-08
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 603079-8
    ISSN 1878-5905 ; 0142-9612
    ISSN (online) 1878-5905
    ISSN 0142-9612
    DOI 10.1016/j.biomaterials.2015.05.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Combinatorial approach for improving the outcome of angiogenic therapy in ischemic tissues

    Odent (Grigorescu), G / A. Burlacu / A.-M. Rosca / D.N. Mitroi / E. Radu / M. Simionescu / M.B. Preda / R. Tutuianu

    Biomaterials. 2015 Aug., v. 60

    2015  

    Abstract: Two major populations of endothelial progenitor cells (EPC), namely endothelial colony forming cells (ECFC, or late outgrowth EPC) and circulating angiogenic cells (CAC, or early outgrowth EPC) have been reported to play important roles in vasculogenesis ...

    Abstract Two major populations of endothelial progenitor cells (EPC), namely endothelial colony forming cells (ECFC, or late outgrowth EPC) and circulating angiogenic cells (CAC, or early outgrowth EPC) have been reported to play important roles in vasculogenesis in numerous pathological conditions. However, the poor retention of cells into the ischemic tissue and neovessel fragility are two major flaws that need to be overcome for successful angiogenic therapy. The objective of this study was to explore and exploit the functional properties of EPC populations in order to increase the effectiveness of post-ischemic cell therapy. The results indicate different, still complementary, effects of the two EPC populations on adherence and proliferation of vascular endothelial cells. Matrigel plug assay and mouse hind limb ischemia model showed that concomitant administration of CAC-secreted factors and ECFC resulted in three-fold increase in local cell retention and improved muscle perfusion, vessel maturation and hind limb regeneration, in comparison to either treatment alone. By concluding, factors secreted by CAC co-administered at the time of ECFC transplantation improve tissue regeneration and vascular repair through stabilization of newly-derived blood vessels.
    Keywords blood vessels ; endothelial cells ; functional properties ; hindlimbs ; ischemia ; mice ; models ; muscles ; stem cells ; therapeutics ; tissue repair ; tissues
    Language English
    Dates of publication 2015-08
    Size p. 72-81.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 603079-8
    ISSN 0142-9612
    ISSN 0142-9612
    DOI 10.1016/j.biomaterials.2015.05.002
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells.

    Odent Grigorescu, Gabriela / Rosca, Ana-Maria / Preda, Mihai Bogdan / Tutuianu, Raluca / Simionescu, Maya / Burlacu, Alexandrina

    Journal of tissue engineering and regenerative medicine

    2016  Volume 11, Issue 11, Page(s) 3241–3252

    Abstract: Here we investigated the impact of hypoxic environment on the angiogenic properties of early-outgrowth endothelial progenitor cells (EPCs), with particular focus on the role of secreted vascular endothelial growth factor-A (VEGF-A) and stromal derived ... ...

    Abstract Here we investigated the impact of hypoxic environment on the angiogenic properties of early-outgrowth endothelial progenitor cells (EPCs), with particular focus on the role of secreted vascular endothelial growth factor-A (VEGF-A) and stromal derived factor-1 (SDF-1) in mediating these effects. We found that cultured EPCs secreted factors with paracrine effects on chemotaxis, migration, proliferation and tube formation of mature endothelial cells (ECs), and these properties were not affected by hypoxia. Depletion of VEGF-A did not change the ability of EPC-conditioned medium (CM) to promote EC migration and tube formation in vitro, suggesting that the pro-angiogenic paracrine effects of EPCs did not totally rely on the presence of VEGF-A. These findings were confirmed by in vivo experiments, on a mouse model of hind limb ischaemia, which showed that VEGF-depleted EPC-CM sustained tissue perfusion at the same level as complete EPC-CM. However, concomitant deletion of VEGF-A and SDF-1 in EPC-CM impaired the pro-angiogenic properties of EPC-CM, by inhibition of EC spreading in culture, tube-like structure formation on Matrigel support, in vivo neovessels formation and ischaemic hind limb regeneration. Taken together, our data demonstrate that: (i) hypoxia does not affect the capacity of EPCs to support the angiogenic process; (ii) the absence of either VEGF-A or SDF-1 from EPC-CM can be rescued by the presence of the other one, so that the overall angiogenic effects remain unchanged; and (iii) and the concomitant deletion of VEGF-A and SDF-1 from EPC-CM impairs its pro-angiogenic effect, both in vitro and in vivo. Copyright © 2016 John Wiley & Sons, Ltd.
    MeSH term(s) Animals ; Chemokine CXCL12/agonists ; Chemokine CXCL12/metabolism ; Endothelial Progenitor Cells/metabolism ; Hindlimb/blood supply ; Hindlimb/metabolism ; Humans ; Ischemia/metabolism ; Ischemia/therapy ; Mice ; Neovascularization, Physiologic ; Vascular Endothelial Growth Factor A/agonists ; Vascular Endothelial Growth Factor A/metabolism
    Chemical Substances CXCL12 protein, human ; Chemokine CXCL12 ; VEGFA protein, human ; Vascular Endothelial Growth Factor A
    Language English
    Publishing date 2016-12-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-7005
    ISSN (online) 1932-7005
    DOI 10.1002/term.2233
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Polyvinylamine hydrochloride-based microcapsules: polymer synthesis, permeability and mechanical properties.

    Grigorescu, G / Rehor, A / Hunkeler, D

    Journal of microencapsulation

    2002  Volume 19, Issue 2, Page(s) 245–259

    Abstract: Mechanically stable microcapsules, with sizes of 0.4-1.5 mm, have been produced with permeabilities appropriate for applications involving living cells and controlled delivery. Polyvinylamine hydrochloride was employed alone, in miXtures with poly( ... ...

    Abstract Mechanically stable microcapsules, with sizes of 0.4-1.5 mm, have been produced with permeabilities appropriate for applications involving living cells and controlled delivery. Polyvinylamine hydrochloride was employed alone, in miXtures with poly(methylene-co-guanidine) hydrochloride, or as a coating material for pre-formed capsules. The influence of polymer molar mass, the ratio between the two polycations, the coating time, and the capsule size on the properties of the capsules were analysed. The competitive displacement of one polycation with another in the polysaccharide matrix was also documented. The properties of the capsules vary remarkably, depending of the polyelectrolyte combinations used for their preparation. Specifically, capsules could withstand compressive loads of between 0.09-1.67 N, while the permeability varied from 10-120 kDa. Both are within the ranges required for clinical immunosuppressive therapies.
    MeSH term(s) Capsules/chemistry ; Capsules/pharmacokinetics ; Coated Materials, Biocompatible/chemistry ; Coated Materials, Biocompatible/pharmacokinetics ; Drug Stability ; Guanidines ; Particle Size ; Permeability ; Polyamines ; Polyvinyls/chemical synthesis ; Polyvinyls/pharmacokinetics
    Chemical Substances Capsules ; Coated Materials, Biocompatible ; Guanidines ; Polyamines ; Polyvinyls ; poly(methylene-co-guanidine) ; poly(vinylamine) (26336-38-9)
    Language English
    Publishing date 2002-01-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 843632-0
    ISSN 1464-5246 ; 0265-2048
    ISSN (online) 1464-5246
    ISSN 0265-2048
    DOI 10.1080/02652040110081361
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    Zs.A 2267: Show issues Location:
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  6. Article: Diagnosticul de laborator al icterelor.

    Grigorescu, G

    Munca sanitara

    1971  Volume 19, Issue 11, Page(s) 662–667

    Title translation Laboratory diagnosis of icterus.
    MeSH term(s) Jaundice/diagnosis
    Language Romanian
    Publishing date 1971-11
    Publishing country Romania
    Document type Journal Article
    ZDB-ID 415059-4
    ISSN 0027-318X
    ISSN 0027-318X
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    Zs.B 1453: Show issues Location:
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  7. Article: Aspecte inedite ale unui caz de rinofimă.

    Obreja, S / Grigorescu, G

    Revista de chirurgie, oncologie, radiologie, o.r.l., oftalmologie, stomatologie. Oto-rino-laringologia

    1989  Volume 34, Issue 2, Page(s) 133–138

    Abstract: The present paper reports on a case of rhinophyma associated with pendulous fibroma, operated in the same surgical sequence, with grafting the decortication surface of the pyramid with graft of free skin taken from the arm. The clinical and histological ... ...

    Title translation Unusual aspects of a case of rhinophyma.
    Abstract The present paper reports on a case of rhinophyma associated with pendulous fibroma, operated in the same surgical sequence, with grafting the decortication surface of the pyramid with graft of free skin taken from the arm. The clinical and histological characteristics of the case presented and the conclusions to be drawn from it are outlined, as the disease is a nosologic entity rarely met.
    MeSH term(s) Aged ; Fibroma/diagnosis ; Fibroma/pathology ; Fibroma/surgery ; Humans ; Male ; Nose Neoplasms/diagnosis ; Nose Neoplasms/pathology ; Nose Neoplasms/surgery ; Rhinophyma/diagnosis ; Rhinophyma/pathology ; Rhinophyma/surgery ; Rosacea ; Skin Transplantation
    Language Romanian
    Publishing date 1989-04
    Publishing country Romania
    Document type Case Reports ; English Abstract ; Journal Article
    ISSN 1220-0867
    ISSN 1220-0867
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Factors secreted by mesenchymal stem cells and endothelial progenitor cells have complementary effects on angiogenesis in vitro.

    Burlacu, Alexandrina / Grigorescu, Gabriela / Rosca, Ana-Maria / Preda, Mihai Bogdan / Simionescu, Maya

    Stem cells and development

    2012  Volume 22, Issue 4, Page(s) 643–653

    Abstract: Stem cell-based therapy for myocardial regeneration has reported several functional improvements that are attributed mostly to the paracrine effects stimulating angiogenesis and cell survival. This study was conducted to comparatively evaluate the ... ...

    Abstract Stem cell-based therapy for myocardial regeneration has reported several functional improvements that are attributed mostly to the paracrine effects stimulating angiogenesis and cell survival. This study was conducted to comparatively evaluate the potential of factors secreted by mesenchymal stem cells (MSCs) in normoxic and hypoxic conditions to promote tissue repair by sustaining endothelial cell (EC) adhesion and proliferation and conferring protection against apoptosis. To this aim, a conditioned medium (CM) was generated from MSCs after 24-h incubation in a serum-free normal or hypoxic environment. MSCs exhibited resistance to hypoxia, which induced increased secretion of vascular endothelial growth factor (VEGF) and decreased levels of other cytokines, including stromal-derived factor-1 (SDF). The CM derived from normal (nMSC-CM) and hypoxic cells (hypMSC-CM) induced similar protective effects on H9c2 cells in hypoxia. Minor differences were noticed in the potential of normal versus hypoxic CM to promote angiogenesis, which were likely connected to SDFα and VEGF levels: the nMSC-CM was more effective in stimulating EC migration, whereas the hypMSC-CM had an enhanced effect on EC adhesion. However, the factors secreted by MSCs in normoxic or hypoxic conditions supported adhesion, but not proliferation, of ECs in vitro, as revealed by impedance-based dynamic assessments. Surprisingly, factors secreted by other stem/progenitor cells, such as endothelial progenitor cells (EPCs), had complementary effects to the MSC-CM. Thus, the EPC-CM, in either a normal or hypoxic environment, supported EC proliferation, but did not sustain EC adhesion. Combined use of the MSC-CM and EPC-CM promoted both EC adhesion and proliferation, suggesting that the local angiogenesis at the site of ischemic injury might be better stimulated by simultaneous releasing of factors secreted by multiple stem/progenitor cell populations.
    MeSH term(s) Animals ; Cell Adhesion/drug effects ; Cell Adhesion/physiology ; Cell Hypoxia/drug effects ; Cell Hypoxia/physiology ; Cell Line ; Cell Proliferation ; Chemokine CXCL12/metabolism ; Culture Media, Conditioned/pharmacology ; Endothelial Cells/cytology ; Endothelial Cells/metabolism ; Female ; Humans ; Male ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Mice ; Neovascularization, Physiologic/drug effects ; Neovascularization, Physiologic/physiology ; Vascular Endothelial Growth Factor A/metabolism
    Chemical Substances CXCL12 protein, human ; Chemokine CXCL12 ; Culture Media, Conditioned ; VEGFA protein, human ; Vascular Endothelial Growth Factor A
    Language English
    Publishing date 2012-10-19
    Publishing country United States
    Document type Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2142214-X
    ISSN 1557-8534 ; 1547-3287
    ISSN (online) 1557-8534
    ISSN 1547-3287
    DOI 10.1089/scd.2012.0273
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    Zs.A 3616: Show issues Location:
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  9. Article: Ligatura de carotidă externă bilaterală într-un caz de hemoragie post-amigdalectomie.

    Obreja, S / Grigorescu, G

    Revista de chirurgie, oncologie, radiologie, o.r.l., oftalmologie, stomatologie. Oto-rino-laringologia

    1988  Volume 33, Issue 2, Page(s) 145–147

    Title translation Bilateral ligation of the external carotid in a case of post-tonsillectomy hemorrhage.
    MeSH term(s) Adult ; Carotid Artery, External/surgery ; Emergencies ; Female ; Hemorrhage/pathology ; Hemorrhage/surgery ; Humans ; Ligation ; Postoperative Complications/pathology ; Postoperative Complications/surgery ; Tonsillectomy/adverse effects
    Language Romanian
    Publishing date 1988-04
    Publishing country Romania
    Document type Case Reports ; English Abstract ; Journal Article
    ISSN 1220-0867
    ISSN 1220-0867
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  10. Article: Premorbid cognitive and behavioral functioning in military recruits experiencing the first episode of psychosis.

    Gheorge, Mihai D / Baloescu, Alexandrina / Grigorescu, Gabriela

    CNS spectrums

    2004  Volume 9, Issue 8, Page(s) 604–606

    Abstract: Objective: To characterize the premorbid cognitive and behavioral abilities in apparently healthy adolescents who at a later time will be diagnosed with schizophreniform disorder or schizophrenia.: Background: Clarifying the pathological relationship ...

    Abstract Objective: To characterize the premorbid cognitive and behavioral abilities in apparently healthy adolescents who at a later time will be diagnosed with schizophreniform disorder or schizophrenia.
    Background: Clarifying the pathological relationship between subtle intellectual and behavioral abnormalities and disease could provide markers for the early prediction of future psychosis.
    Method: Premorbid data on young male patients admitted to the Department of Psychiatry of the Central Military Hospital in Bucharest, Romania, between 1996 and 2002 and diagnosed with a first episode of psychosis or schizophreniform disorder were collected. The premorbid data consisted in the test scores of intellectual functioning and personality traits were collected by the Romanian Draft Board in order to assesses their aptitude to serve in the military. Premorbid cognitive and behavioral scores of male patients (cases=157) were compared with the scores of healthy male individuals (non-cases=169) matched for age, education, and geographic area of residence. The tests were administered when subjects were 18 years of age (initial screening) and the entire assessment was completed and concluded when subjects reached 20 years of age (actual conscription).
    Results: As a group, apparently healthy males later admitted for a first episode of psychosis or schizophreniform disorder, obtained lower (worse) scores on the Raven Progressive Matrices test and on relevant personality traits in comparison to controls.
    Conclusion: The results add to the accumulating body of evidence suggesting that aspects of schizophrenia manifest years before the illness is formally diagnosed. Despite these results, more studies are needed to improve the diagnosing specificity and predictive value of the premorbid cognitive and behavioral manifestations, before they can be used as markers in models of primary or secondary prevention.
    MeSH term(s) Adaptation, Psychological ; Adult ; Cognition Disorders/diagnosis ; Cognition Disorders/psychology ; Cohort Studies ; Diagnosis, Differential ; Early Diagnosis ; Humans ; Male ; Mental Disorders/diagnosis ; Mental Disorders/psychology ; Military Personnel/psychology ; Romania ; Schizophrenia/diagnosis ; Schizophrenic Psychology ; Schizotypal Personality Disorder/diagnosis ; Schizotypal Personality Disorder/psychology
    Language English
    Publishing date 2004-07-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2008418-3
    ISSN 2165-6509 ; 1092-8529
    ISSN (online) 2165-6509
    ISSN 1092-8529
    DOI 10.1017/s1092852900002777
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    Zs.MO 340: Show issues

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