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  1. Article ; Online: Pediatric residents' perceptions about the impact of the COVID-19 pandemic on their training.

    Labanca, Vanesa D / Giacomossi, Pedro / Urtasun, Marcela / Grisolia, Nicolás A / Domínguez, Paula

    Archivos argentinos de pediatria

    2023  Volume 122, Issue 1, Page(s) e202310059

    Abstract: Introduction. Medical residency programs suffered changes that may have affected academic training during the COVID-19 pandemic. Objectives. To describe the perceptions of pediatric residents about the pandemic's impact on their education. To ... ...

    Title translation Percepción de los médicos residentes de Pediatría sobre el impacto de la pandemia por COVID-19 en su proceso formativo.
    Abstract Introduction. Medical residency programs suffered changes that may have affected academic training during the COVID-19 pandemic. Objectives. To describe the perceptions of pediatric residents about the pandemic's impact on their education. To transculturally adapt and validate the COVID-19 Resident Education and Experience Survey into Spanish. Materials and methods. Observational, cross-sectional study. Participants were pediatric residents from hospitals across the country. The survey by Ostapenko et al. was used. A descriptive analysis was done using the SPSS software, version 21. The project was approved by the Institutional Ethics Committee. Results. The survey was completed by 127 residents. Most did more than 50 hours of health care activities before and during the pandemic. Also, 43.3% (95% CI: 35-52) spent at least 1 hour a day studying individually before the pandemic, while 63% (95% CI: 54.3-70.9) did so during the pandemic. In relation to the time spent doing academic work, 75.6% (95% CI: 67.4-82.2) reported that, before the pandemic, they spent at least 4 hours a week doing academic activities, dropping to 41.7% (95% CI: 33.5-50.4) during the pandemic. More than 60% (95% CI: 54.3-70.1) perceived that the pandemic impaired their training to become a specialist and 93.7% (95% CI: 88.1-96.8), that their stress levels increased. Conclusions. The perception was that participants spent less hours doing academic activities during the pandemic. Most surveyed participants mentioned that their stress levels increased and that the pandemic impaired their training to become a specialist.
    MeSH term(s) Humans ; Child ; COVID-19/epidemiology ; Pandemics ; Cross-Sectional Studies ; Surveys and Questionnaires ; Internship and Residency
    Language Spanish
    Publishing date 2023-08-31
    Publishing country Argentina
    Document type Journal Article
    ZDB-ID 424449-7
    ISSN 1668-3501 ; 0325-0075 ; 0004-0487
    ISSN (online) 1668-3501
    ISSN 0325-0075 ; 0004-0487
    DOI 10.5546/aap.2023-10059.eng
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Screening for common respiratory viruses in pediatric outpatients 2 years after the onset of the COVID-19 pandemic.

    Leone, María B / Ponti, Daniela A / Fernández Berengeno, María N / Grisolía, Nicolás A / Aprea, Valeria P / Yazde de Puleio, María L / Svartz, Alejandra / Haleblian, Emilce

    Archivos argentinos de pediatria

    2022  Volume 120, Issue 4, Page(s) 264–268

    Abstract: Introduction: During 2020, circulation of other respiratory viruses was lower than usual. Most likely, as mitigation measures for coronavirus disease 2019 (COVID-19) were modified, their prevalence in 2021 may have increased.: Objective: To estimate ... ...

    Title translation Pesquisa de virus respiratorios habituales en pacientes pediátricos ambulatorios, a dos años de la pandemia por COVID-19.
    Abstract Introduction: During 2020, circulation of other respiratory viruses was lower than usual. Most likely, as mitigation measures for coronavirus disease 2019 (COVID-19) were modified, their prevalence in 2021 may have increased.
    Objective: To estimate the prevalence of common respiratory viruses among patients aged 0-5 years seen at the Emergency Department of a children's hospital in the City of Buenos Aires.
    Methods: Cross-sectional study of 348 patients consulting for suspected COVID-19 in whom SARS-CoV-2 infection was ruled out and routine screening for common respiratory viruses was performed.
    Results: Respiratory syncytial virus (RSV), a common respiratory virus, was identified in 40% of patients. Age younger than 2 years was an independent predictor of RSV (odds ratio [OR]: 4.15; 95% confidence interval [CI]: 2.46-6.99).
    Conclusions: . In the study population, 40% of patients suspected of COVID-19 in whom SARS-CoV-2 infection was ruled out had RSV infection.
    MeSH term(s) COVID-19/diagnosis ; COVID-19/epidemiology ; Child ; Cross-Sectional Studies ; Humans ; Outpatients ; Pandemics ; Respiratory Syncytial Virus, Human ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/epidemiology ; SARS-CoV-2 ; Viruses
    Language Spanish
    Publishing date 2022-05-04
    Publishing country Argentina
    Document type Journal Article
    ZDB-ID 424449-7
    ISSN 1668-3501 ; 0325-0075 ; 0004-0487
    ISSN (online) 1668-3501
    ISSN 0325-0075 ; 0004-0487
    DOI 10.5546/aap.2022.eng.264
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Usefulness of an early warning score as an early predictor of clinical deterioration in hospitalized children.

    Elencwajg, Magalí / Grisolía, Nicolás A / Meregalli, Claudia / Montecuco, Micaela A / Montiel, María V / Rodríguez, Gabriela M / Serviddio, Carla C

    Archivos argentinos de pediatria

    2020  Volume 118, Issue 6, Page(s) 399–404

    Abstract: Introduction: The early detection of clinical deterioration in hospitalized patients helps to improve the quality of care. The pediatric early warning score (PEWS) system predicts such deterioration in the first 24 hours of administration. Prior studies ...

    Title translation Utilidad de una escala de alerta temprana como predictor precoz de deterioro clínico en niños internados.
    Abstract Introduction: The early detection of clinical deterioration in hospitalized patients helps to improve the quality of care. The pediatric early warning score (PEWS) system predicts such deterioration in the first 24 hours of administration. Prior studies support the use of these tools.
    Objective: To assess the usefulness of the Brighton PEWS (B-PEWS) for the prediction of clinical deterioration among hospitalized children at a children's hospital in the Autonomous City of Buenos Aires.
    Population and methods: Cross-sectional study. The medical records of all patients aged 1 month to 18 years admitted to any hospitalization ward between March 1st and August 31st, 2018 were included. Predictive outcome measure: B-PEWS score (≤ 3 and ≥ 4 points). Outcome variable: clinical deterioration in the first 24 hours. Categorical outcome measures were analyzed with the χ² test and screening values were estimated. The relative risk was used as a measure of association. A ROC curve analysis and an optimal cut-point analysis according to the Youden index were done considering the score in a continuous manner.
    Results: A total of 518 medical records were reviewed. Forty patients had clinical deterioration; the B-PEWS score was ≥ 4 in 37 patients and ≤ 3 in 3 (relative risk: 56.36; 95 % confidence interval: 17.76-178.89; p < 0.01). Sensitivity: 92.5 %; specificity: 88.3 %; positive predictive value: 39.8 %; negative predictive value: 99.3 %; positive likelihood ratio: 7.91; negative likelihood ratio: 0.08. AUC: 0.94 (95 % confidence interval: 0.89-0.98).
    Conclusions: The B-PEWS demonstrated to be useful to predict clinical deterioration in hospitalized children.
    MeSH term(s) Child ; Child, Hospitalized ; Clinical Deterioration ; Cross-Sectional Studies ; Early Warning Score ; Hospitalization ; Humans ; Retrospective Studies
    Language Spanish
    Publishing date 2020-11-23
    Publishing country Argentina
    Document type Journal Article
    ZDB-ID 424449-7
    ISSN 1668-3501 ; 0325-0075 ; 0004-0487
    ISSN (online) 1668-3501
    ISSN 0325-0075 ; 0004-0487
    DOI 10.5546/aap.2020.eng.399
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers.

    Seery, Vanesa / Raiden, Silvina C / Algieri, Silvia C / Grisolía, Nicolás A / Filippo, Daniela / De Carli, Norberto / Di Lalla, Sandra / Cairoli, Héctor / Chiolo, María J / Meregalli, Claudia N / Gimenez, Lorena I / Gregorio, Gabriela / Sarli, Mariam / Alcalde, Ana L / Davenport, Carolina / Bruera, María J / Simaz, Nancy / Pérez, Mariela F / Nivela, Valeria /
    Bayle, Carola / Tuccillo, Patricia / Agosta, María T / Pérez, Hernán / Villa Nova, Susana / Suárez, Patricia / Takata, Eugenia M / García, Mariela / Lattner, Jorge / Rolón, María J / Coll, Patricia / Sananez, Inés / Holgado, María P / Ferrero, Fernando / Geffner, Jorge / Arruvito, Lourdes

    EBioMedicine

    2021  Volume 67, Page(s) 103357

    Abstract: Background: Perhaps reflecting that children with COVID-19 rarely exhibit severe respiratory symptoms and often remain asymptomatic, little attention has been paid to explore the immune response in pediatric COVID-19. Here, we analyzed the phenotype and ...

    Abstract Background: Perhaps reflecting that children with COVID-19 rarely exhibit severe respiratory symptoms and often remain asymptomatic, little attention has been paid to explore the immune response in pediatric COVID-19. Here, we analyzed the phenotype and function of circulating neutrophils from children with COVID-19.
    Methods: An observational study including 182 children with COVID-19, 21 children with multisystem inflammatory syndrome (MIS-C), and 40 healthy children was performed in Buenos Aires, Argentina. Neutrophil phenotype was analyzed by flow cytometry in blood samples. Cytokine production, plasma levels of IgG antibodies directed to the spike protein of SARS-CoV-2 and citrullinated histone H3 were measured by ELISA. Cell-free DNA was quantified by fluorometry.
    Findings: Compared with healthy controls, neutrophils from children with COVID-19 showed a lower expression of CD11b, CD66b, and L-selectin but a higher expression of the activation markers HLA-DR, CD64 and PECAM-1 and the inhibitory receptors LAIR-1 and PD-L1. No differences in the production of cytokines and NETs were observed. Interestingly, the expression of CD64 in neutrophils and the serum concentration of IgG antibodies directed to the spike protein of SARS-CoV-2 distinguished asymptomatic from mild and moderate COVID-19.
    Interpretation: Acute lung injury is a prominent feature of severe COVID-19 in adults. A low expression of adhesion molecules together with a high expression of inhibitory receptors in neutrophils from children with COVID-19 might prevent tissue infiltration by neutrophils preserving lung function.
    Funding: This study was supported by the Ministry of Science and Technology (National Agency for Scientific and Technological Promotion, IP-COVID-19-0277 and PMO BID PICT 2018-2548), and University of Buenos Aires from Argentina (20020170100573BA).
    MeSH term(s) Antibodies, Viral/blood ; Argentina ; Biomarkers/blood ; COVID-19/blood ; COVID-19/immunology ; Case-Control Studies ; Child ; Child, Preschool ; Cytokines/blood ; Female ; Flow Cytometry ; Humans ; Immunoglobulin G/blood ; Infant ; Male ; Neutrophils/immunology ; SARS-CoV-2/immunology ; Spike Glycoprotein, Coronavirus/immunology ; Systemic Inflammatory Response Syndrome/blood ; Systemic Inflammatory Response Syndrome/immunology
    Chemical Substances Antibodies, Viral ; Biomarkers ; Cytokines ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2021-05-09
    Publishing country Netherlands
    Document type Journal Article ; Observational Study
    ZDB-ID 2851331-9
    ISSN 2352-3964
    ISSN (online) 2352-3964
    DOI 10.1016/j.ebiom.2021.103357
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A poor and delayed anti-SARS-CoV2 IgG response is associated to severe COVID-19 in children.

    Sananez, Inés / Raiden, Silvina C / Algieri, Silvia C / Uranga, Macarena / Grisolía, Nicolás A / Filippo, Daniela / De Carli, Norberto / Lalla, Sandra Di / Cairoli, Héctor / Chiolo, María J / Meregalli, Claudia N / Cohen, Emilia / Mosquera, Graciela / Marcó Del Pont, María / Giménez, Lorena I / Gregorio, Gabriela / Sarli, Mariam / Alcalde, Ana L / Davenport, Carolina /
    Bruera, María J / Simaz, Nancy / Pérez, Mariela F / Nivela, Valeria / Bayle, Carola / Alvarez, Laura / Revetria, María / Tuccillo, Patricia / Agosta, María T / Pérez, Hernán / Nova, Susana Villa / Suárez, Patricia / Takata, Eugenia M / García, Mariela / Lattner, Jorge / Rolón, María J / Coll, Patricia / Salvatori, Melina / Piccardo, Claudio / Russo, Constanza / Varese, Augusto / Seery, Vanesa / Holgado, María P / Polo, María L / Ceballos, Ana / Nuñez, Myriam / Penedo, Juan Martín Gómez / Ferrero, Fernando / Geffner, Jorge / Arruvito, Lourdes

    EBioMedicine

    2021  Volume 72, Page(s) 103615

    Abstract: Background: Most children and youth develop mild or asymptomatic disease during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, a very small number of patients suffer severe Coronavirus induced disease 2019 (COVID-19). ... ...

    Abstract Background: Most children and youth develop mild or asymptomatic disease during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, a very small number of patients suffer severe Coronavirus induced disease 2019 (COVID-19). The reasons underlying these different outcomes remain unknown.
    Methods: We analyzed three different cohorts: children with acute infection (n=550), convalescent children (n=138), and MIS-C (multisystem inflammatory syndrome in children, n=42). IgG and IgM antibodies to the spike protein of SARS-CoV-2, serum-neutralizing activity, plasma cytokine levels, and the frequency of circulating Follicular T helper cells (cTfh) and plasmablasts were analyzed by conventional methods.
    Findings: Fifty-eight percent of the children in the acute phase of infection had no detectable antibodies at the time of sampling while a seronegative status was found in 25% and 12% of convalescent and MIS-C children, respectively. When children in the acute phase of the infection were stratified according disease severity, we found that contrasting with the response of children with asymptomatic, mild and moderate disease, children with severe COVID-19 did not develop any detectable response. A defective antibody response was also observed in the convalescent cohort for children with severe disease at the time of admission. This poor antibody response was associated to both, a low frequency of cTfh and a high plasma concentration of inflammatory cytokines.
    Interpretation: A weak and delayed kinetic of antibody response to SARS-CoV-2 together with a systemic pro-inflammatory profile characterize pediatric severe COVID-19. Because comorbidities are highly prevalent in children with severe COVID-19, further studies are needed to clarify their contribution in the weak antibody response observed in severe disease.
    Funding: National Agency for Scientific and Technological Promotion from Argentina (IP-COVID-19-0277 and PMO-BID-PICT2018-2548).
    MeSH term(s) Antibodies, Viral/blood ; Antibody Formation ; Argentina ; COVID-19/blood ; COVID-19/complications ; COVID-19/immunology ; Child ; Child, Preschool ; Cytokines/blood ; Female ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Infant ; Male ; SARS-CoV-2/immunology ; Systemic Inflammatory Response Syndrome/blood ; Systemic Inflammatory Response Syndrome/immunology
    Chemical Substances Antibodies, Viral ; Cytokines ; Immunoglobulin G ; Immunoglobulin M
    Language English
    Publishing date 2021-10-11
    Publishing country Netherlands
    Document type Journal Article ; Observational Study
    ZDB-ID 2851331-9
    ISSN 2352-3964
    ISSN (online) 2352-3964
    DOI 10.1016/j.ebiom.2021.103615
    Database MEDical Literature Analysis and Retrieval System OnLINE

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