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  1. Article ; Online: Regenerative Medizin für das Hornhautepithel : Zelltherapie in Wissenschaft und Klinik.

    Menzel-Severing, Johannes / Spaniol, Kristina / Groeber-Becker, Florian / Geerling, Gerd

    Die Ophthalmologie

    2022  Volume 119, Issue 9, Page(s) 891–901

    Abstract: In the case of thermal or caustic burns of the ocular surface, loss of limbal epithelial stem cells leads to compromised self-renewal of the corneal epithelium. This results in permanent loss of vision. In these situations, transplantation of cultured ... ...

    Title translation Regenerative medicine for the corneal epithelium : Cell therapy from bench to bedside.
    Abstract In the case of thermal or caustic burns of the ocular surface, loss of limbal epithelial stem cells leads to compromised self-renewal of the corneal epithelium. This results in permanent loss of vision. In these situations, transplantation of cultured limbal epithelial cells on an amniotic membrane or fibrin gel as substrate (Holoclar®) can help to regenerate the corneal surface. The required cells are obtained from the healthy partner eye, if available. Adult stem cells from other parts of the body potentially serve as alternative cell sources: hair follicles, oral mucosa, mesenchymal stromal cells, or induced pluripotent stem cells (originally, e.g., skin fibroblasts). The reprogramming of such cells can be achieved with the help of transcription factors. In addition, work is being done on biosynthetic or synthetic matrices, which not only serve as substrate material for the transplantation but also support the functional properties of these cells (self-renewal, corneal epithelial-typical phenotype).
    MeSH term(s) Burns, Chemical/metabolism ; Cell- and Tissue-Based Therapy ; Cornea ; Epithelium, Corneal ; Humans ; Regenerative Medicine
    Language German
    Publishing date 2022-06-24
    Publishing country Germany
    Document type Journal Article ; Review
    ISSN 2731-7218
    ISSN (online) 2731-7218
    DOI 10.1007/s00347-022-01674-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Online ; Thesis: Lack of NFATc1 SUMOylation prevents autoimmunity and alloreactivity

    Xiao, Yin [Verfasser] / Krappman, Daniel [Gutachter] / Kastenmüller, Wolfgang [Gutachter] / Rosenwald, Andreas [Gutachter] / Groeber-Becker, Florian [Gutachter]

    2023  

    Author's details Yin Xiao ; Gutachter: Daniel Krappman, Wolfgang Kastenmüller, Andreas Rosenwald, Florian Groeber-Becker
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  3. Book ; Online ; Thesis: Development of a tissue-engineered primary human skin infection model to study the pathogenesis of tsetse fly-transmitted African trypanosomes in mammalian skin

    Reuter, Christian Steffen [Verfasser] / Engstler, Markus [Gutachter] / Walles, Heike [Gutachter] / Groeber-Becker, Florian [Gutachter]

    2023  

    Author's details Christian Steffen Reuter ; Gutachter: Markus Engstler, Heike Walles, Florian Groeber-Becker
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  4. Article ; Online: Fully Synthetic 3D Fibrous Scaffolds for Stromal Tissues-Replacement of Animal-Derived Scaffold Materials Demonstrated by Multilayered Skin.

    Weigel, Tobias / Malkmus, Christoph / Weigel, Verena / Wußmann, Maximiliane / Berger, Constantin / Brennecke, Julian / Groeber-Becker, Florian / Hansmann, Jan

    Advanced materials (Deerfield Beach, Fla.)

    2022  Volume 34, Issue 10, Page(s) e2106780

    Abstract: The extracellular matrix (ECM) of soft tissues in vivo has remarkable biological and structural properties. Thereby, the ECM provides mechanical stability while it still can be rearranged via cellular remodeling during tissue maturation or healing ... ...

    Abstract The extracellular matrix (ECM) of soft tissues in vivo has remarkable biological and structural properties. Thereby, the ECM provides mechanical stability while it still can be rearranged via cellular remodeling during tissue maturation or healing processes. However, modern synthetic alternatives fail to provide these key features among basic properties. Synthetic matrices are usually completely degraded or are inert regarding cellular remodeling. Based on a refined electrospinning process, a method is developed to generate synthetic scaffolds with highly porous fibrous structures and enhanced fiber-to-fiber distances. Since this approach allows for cell migration, matrix remodeling, and ECM synthesis, the scaffold provides an ideal platform for the generation of soft tissue equivalents. Using this matrix, an electrospun-based multilayered skin equivalent composed of a stratified epidermis, a dermal compartment, and a subcutis is able to be generated without the use of animal matrix components. The extension of classical dense electrospun scaffolds with high porosities and motile fibers generates a fully synthetic and defined alternative to collagen-gel-based tissue models and is a promising system for the construction of tissue equivalents as in vitro models or in vivo implants.
    MeSH term(s) Animals ; Connective Tissue ; Extracellular Matrix/chemistry ; Skin ; Tissue Engineering ; Tissue Scaffolds/chemistry
    Language English
    Publishing date 2022-01-21
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1474949-X
    ISSN 1521-4095 ; 0935-9648
    ISSN (online) 1521-4095
    ISSN 0935-9648
    DOI 10.1002/adma.202106780
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  5. Book ; Online: Regenerative Medizin für das Hornhautepithel

    Menzel-Severing, Johannes / Spaniol, Kristina / Groeber-Becker, Florian Kai / Geerling, Gerd

    Zelltherapie in Wissenschaft und Klinik ; Regenerative medicine for the corneal epithelium. Cell therapy from bench to bedside

    2022  

    Abstract: 891 ... 901 ... Bei einer Verbrennung oder Verätzung der Augenoberfläche kann der Verlust von Limbusepithelstammzellen zu einer kompromittierten Selbsterneuerung des Hornhautepithels führen. Hierdurch kommt es zu einem dauerhaften Sehverlust. In diesen ... ...

    Abstract 891

    901

    Bei einer Verbrennung oder Verätzung der Augenoberfläche kann der Verlust von Limbusepithelstammzellen zu einer kompromittierten Selbsterneuerung des Hornhautepithels führen. Hierdurch kommt es zu einem dauerhaften Sehverlust. In diesen Situationen kann die Transplantation von kultivierten Limbusepithelzellen auf einer Amnionmembran oder einem Fibringel als Träger (Holoclar®, Holostem Terapie Avanzate s.r.l., Modena, Italien) zu einer Regeneration der Hornhautoberfläche beitragen. Die erforderlichen Zellen werden dem gesunden Partnerauge entnommen – sofern vorhanden. Alternative Zellquellen sind adulte Stammzellen aus anderen Körperregionen (Haarfollikel, Mundschleimhaut, mesenchymale Stromazellen) oder induzierte pluripotente Stammzellen (ursprünglich z. B. Hautfibroblasten). Das Umprogrammieren solcher Zellen kann unter Zuhilfenahme von Transkriptionsfaktoren erfolgen. Zusätzlich wird an biosynthetischen oder synthetischen Matrices gearbeitet, die nicht nur als Trägermaterial für die Transplantation dienen, sondern auch die Funktionen der Zellen (Selbsterneuerung, hornhautepitheltypischer Phänotyp) unterstützen sollen.

    In the case of thermal or caustic burns of the ocular surface, loss of limbal epithelial stem cells leads to compromised self-renewal of the corneal epithelium. This results in permanent loss of vision. In these situations, transplantation of cultured limbal epithelial cells on an amniotic membrane or fibrin gel as substrate (Holoclar®) can help to regenerate the corneal surface. The required cells are obtained from the healthy partner eye, if available. Adult stem cells from other parts of the body potentially serve as alternative cell sources: hair follicles, oral mucosa, mesenchymal stromal cells, or induced pluripotent stem cells (originally, e.g., skin fibroblasts). The reprogramming of such cells can be achieved with the help of transcription factors. In addition, work is being done on biosynthetic or synthetic matrices, which not only serve as substrate material for the ...
    Keywords Limbusstammzellinsuffizienz ; Limbusstammzelltransplantation ; Tissue-Engineering ; Umprogrammierung ; Matrices ; DDC::600 Technik ; Medizin ; angewandte Wissenschaften::610 Medizin und Gesundheit::614 Inzidenz und Präventation von Krankheiten
    Subject code 801
    Language German
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Book ; Online ; Thesis: Entwicklung eines \(in\) \(vitro\) Modells für Verbrennungen ersten Grades zur Testung einer zellbasierten Wundauflage ; Gutachter: Florian Groeber-Becker, Markus Engstler, Gerhard Winter

    Weigel [geb. Schneider], Verena [Verfasser] / Groeber-Becker, Florian [Gutachter] / Engstler, Markus [Gutachter] / Winter, Gerhard [Gutachter]

    2022  

    Author's details Verena Weigel [geb. Schneider
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language German
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  7. Book ; Online ; Thesis: Vascularization Strategies for Full-Thickness Skin Equivalents to Model Melanoma Progression

    Leikeim, Anna [Verfasser] / Walles, Heike [Gutachter] / Schmidt, Marc [Gutachter] / Groeber-Becker, Florian [Gutachter] / Groneberg, Dieter [Gutachter]

    2022  

    Author's details Anna Leikeim ; Gutachter: Heike Walles, Marc Schmidt, Florian Groeber-Becker, Dieter Groneberg
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  8. Article ; Online: Digital histology of tissue with Mueller microscopy and FastDBSCAN

    Lee, Hee Ryung / Lotz, Christian / Groeber-Becker, Florian Kai / Dembski, Sofia / Novikova, Tatiana

    2022  

    Abstract: 9616 ... 9624 ... We present the results of the automated post-processing of Mueller microscopy images of skin tissue models with a new fast version of the algorithm of density-based spatial clustering of applications with noise (FastDBSCAN) and discuss the ... ...

    Abstract 9616

    9624

    We present the results of the automated post-processing of Mueller microscopy images of skin tissue models with a new fast version of the algorithm of density-based spatial clustering of applications with noise (FastDBSCAN) and discuss the advantages of its implementation for digital histology of tissue. We demonstrate that using the FastDBSCAN algorithm, one can produce the diagnostic segmentation of high resolution images of tissue by several orders of magnitude faster and with high accuracy (>97%) compared to the original version of the algorithm.

    61

    32
    Keywords Histology ; Skin tissue ; Tissue models ; Microscopy images ; Mueller ; DDC::600 Technik ; Medizin ; angewandte Wissenschaften::610 Medizin und Gesundheit::611 Menschliche Anatomie ; Zytologie ; Histologie
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms.

    Reuter, Christian / Hauf, Laura / Imdahl, Fabian / Sen, Rituparno / Vafadarnejad, Ehsan / Fey, Philipp / Finger, Tamara / Jones, Nicola G / Walles, Heike / Barquist, Lars / Saliba, Antoine-Emmanuel / Groeber-Becker, Florian / Engstler, Markus

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 7660

    Abstract: Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the cell cycle-arrested metacyclic life cycle stage into mammalian skin at the site of the fly's bite. We introduce an advanced human skin equivalent and use tsetse flies to ... ...

    Abstract Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the cell cycle-arrested metacyclic life cycle stage into mammalian skin at the site of the fly's bite. We introduce an advanced human skin equivalent and use tsetse flies to naturally infect the skin with trypanosomes. We detail the chronological order of the parasites' development in the skin by single-cell RNA sequencing and find a rapid activation of metacyclic trypanosomes and differentiation to proliferative parasites. Here we show that after the establishment of a proliferative population, the parasites enter a reversible quiescent state characterized by slow replication and a strongly reduced metabolism. We term these quiescent trypanosomes skin tissue forms, a parasite population that may play an important role in maintaining the infection over long time periods and in asymptomatic infected individuals.
    MeSH term(s) Animals ; Humans ; Trypanosoma brucei brucei/genetics ; Parasites ; Skin/parasitology ; Trypanosoma ; Tsetse Flies/parasitology ; Mammals
    Language English
    Publishing date 2023-11-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-43437-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Biomimetic Connection of Transcutaneous Implants with Skin.

    Weigel, Tobias / Christ, Bastian / Dembski, Sofia / Ewald, Andrea / Groneberg, Dieter / Hansmann, Jan / Luxenhofer, Robert / Metzger, Marco / Walles, Heike / Willy, Christian / Groeber-Becker, Florian / Probst, Jörn

    Advanced healthcare materials

    2023  Volume 12, Issue 30, Page(s) e2301131

    Abstract: Bacterial infection is a crucial complication in implant restoration, in particular in permanent skin-penetrating implants. Therein, the resulting gap between transcutaneous implant and skin represents a permanent infection risk, limiting the field of ... ...

    Abstract Bacterial infection is a crucial complication in implant restoration, in particular in permanent skin-penetrating implants. Therein, the resulting gap between transcutaneous implant and skin represents a permanent infection risk, limiting the field of application and the duration of application. To overcome this limitation, a tight physiological connection is required to achieve a biological and mechanical welding for a long-term stable closure including self-healing probabilities. This study describes a new approach, wherein the implant is connected covalently to a highly porous electrospun fleece featuring physiological dermal integration potential. The integrative potential of the scaffold is shown in vitro and confirmed in vivo, further demonstrating tissue integration by neovascularization, extracellular matrix formation, and prevention of encapsulation. To achieve a covalent connection between fleece and implant surface, self-initiated photografting and photopolymerization of hydroxyethylmethacrylate is combined with a new crosslinker (methacrylic acid coordinated titanium-oxo clusters) on proton-abstractable implant surfaces. For implant modification, the attached fleece is directed perpendicular from the implant surface into the surrounding dermal tissue. First in vitro skin implantations demonstrate the implants' dermal integration capability as well as wound closure potential on top of the fleece by epithelialization, establishing a bacteria-proof and self-healing connection of skin and transcutaneous implant.
    MeSH term(s) Humans ; Biomimetics ; Prostheses and Implants ; Skin ; Titanium ; Neovascularization, Pathologic ; Surface Properties
    Chemical Substances Titanium (D1JT611TNE)
    Language English
    Publishing date 2023-09-13
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649576-4
    ISSN 2192-2659 ; 2192-2640
    ISSN (online) 2192-2659
    ISSN 2192-2640
    DOI 10.1002/adhm.202301131
    Database MEDical Literature Analysis and Retrieval System OnLINE

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