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  1. Article ; Online: Mytilus galloprovincialis CYP1A-like mRNAs reveal closer proximity of mytilid CYP1A to the eumetazoan CYP2 family.

    Cunha, M / Martins, C / Grosso, A R / Costa, P M

    Aquatic toxicology (Amsterdam, Netherlands)

    2019  Volume 214, Page(s) 105260

    Abstract: Due to the role of Cytochrome P450, Family 1, Subfamily A (CYP1A) in the detoxification of many polycyclic aromatic hydrocarbons (PAHs), there has been an effort to characterise the gene and the products from its expression in organisms that are relevant ...

    Abstract Due to the role of Cytochrome P450, Family 1, Subfamily A (CYP1A) in the detoxification of many polycyclic aromatic hydrocarbons (PAHs), there has been an effort to characterise the gene and the products from its expression in organisms that are relevant for biomonitoring and toxicity testing procedures. Nonetheless, the existence of functional homologues in aquatic invertebrates is not entirely consensual, especially in bivalve molluscs, which pose as one of the most important models for aquatic toxicologists, especially mytilids. After isolation and sequencing of CYP1A-like mRNA from the Mediterranean mussel, Mytilus galloprovincialis, phylogenetics incorporating homologues from molluscs and other eumetazoans, vertebrates included, yielded notorious similarity to sequences belonging to the CYP2 Family. Altogether, the findings further indicate that CYP1A-like CYPs may be absent in bivalves, in lieu of Families CYP2, 3 and 4, suggesting caution when interpreting data from common biomarkers of exposure to aromatic hydrocarbons that have been developed for CYP1A activity and expression in higher deuterostomes.
    MeSH term(s) Animals ; Base Sequence ; Cytochrome P-450 CYP1A1/genetics ; Cytochrome P-450 CYP1A1/metabolism ; Cytochrome P450 Family 2/genetics ; Cytochrome P450 Family 2/metabolism ; Mytilus/enzymology ; Mytilus/genetics ; Phylogeny ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Water Pollutants, Chemical/toxicity
    Chemical Substances RNA, Messenger ; Water Pollutants, Chemical ; Cytochrome P-450 CYP1A1 (EC 1.14.14.1) ; Cytochrome P450 Family 2 (EC 1.14.14.1)
    Language English
    Publishing date 2019-07-16
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 782699-0
    ISSN 1879-1514 ; 0166-445X
    ISSN (online) 1879-1514
    ISSN 0166-445X
    DOI 10.1016/j.aquatox.2019.105260
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4376: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  2. Article: Mytilus galloprovincialis CYP1A-like mRNAs reveal closer proximity of mytilid CYP1A to the eumetazoan CYP2 family

    Cunha, M / Martins, C / Grosso, A.R / Costa, P.M

    Aquatic toxicology. 2019 Sept., v. 214

    2019  

    Abstract: Due to the role of Cytochrome P450, Family 1, Subfamily A (CYP1A) in the detoxification of many polycyclic aromatic hydrocarbons (PAHs), there has been an effort to characterise the gene and the products from its expression in organisms that are relevant ...

    Abstract Due to the role of Cytochrome P450, Family 1, Subfamily A (CYP1A) in the detoxification of many polycyclic aromatic hydrocarbons (PAHs), there has been an effort to characterise the gene and the products from its expression in organisms that are relevant for biomonitoring and toxicity testing procedures. Nonetheless, the existence of functional homologues in aquatic invertebrates is not entirely consensual, especially in bivalve molluscs, which pose as one of the most important models for aquatic toxicologists, especially mytilids. After isolation and sequencing of CYP1A-like mRNA from the Mediterranean mussel, Mytilus galloprovincialis, phylogenetics incorporating homologues from molluscs and other eumetazoans, vertebrates included, yielded notorious similarity to sequences belonging to the CYP2 Family. Altogether, the findings further indicate that CYP1A-like CYPs may be absent in bivalves, in lieu of Families CYP2, 3 and 4, suggesting caution when interpreting data from common biomarkers of exposure to aromatic hydrocarbons that have been developed for CYP1A activity and expression in higher deuterostomes.
    Keywords Mytilus galloprovincialis ; aquatic invertebrates ; biomarkers ; cytochrome P-450 ; environmental monitoring ; genes ; messenger RNA ; models ; mussels ; phylogeny ; polycyclic aromatic hydrocarbons ; toxicity testing ; vertebrates
    Language English
    Dates of publication 2019-09
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 782699-0
    ISSN 1879-1514 ; 0166-445X
    ISSN (online) 1879-1514
    ISSN 0166-445X
    DOI 10.1016/j.aquatox.2019.105260
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3881: Show issues

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  3. Article ; Online: Unconstrained mining of transcript data reveals increased alternative splicing complexity in the human transcriptome.

    Mollet, I G / Ben-Dov, Claudia / Felício-Silva, Daniel / Grosso, A R / Eleutério, Pedro / Alves, Ruben / Staller, Ray / Silva, Tito Santos / Carmo-Fonseca, Maria

    Nucleic acids research

    2010  Volume 38, Issue 14, Page(s) 4740–4754

    Abstract: Mining massive amounts of transcript data for alternative splicing information is paramount to help understand how the maturation of RNA regulates gene expression. We developed an algorithm to cluster transcript data to annotated genes to detect ... ...

    Abstract Mining massive amounts of transcript data for alternative splicing information is paramount to help understand how the maturation of RNA regulates gene expression. We developed an algorithm to cluster transcript data to annotated genes to detect unannotated splice variants. A higher number of alternatively spliced genes and isoforms were found compared to other alternative splicing databases. Comparison of human and mouse data revealed a marked increase, in human, of splice variants incorporating novel exons and retained introns. Previously unannotated exons were validated by tiling array expression data and shown to correspond preferentially to novel first exons. Retained introns were validated by tiling array and deep sequencing data. The majority of retained introns were shorter than 500 nt and had weak polypyrimidine tracts. A subset of retained introns matching small RNAs and displaying a high GC content suggests a possible coordination between splicing regulation and production of noncoding RNAs. Conservation of unannotated exons and retained introns was higher in horse, dog and cow than in rodents, and 64% of exon sequences were only found in primates. This analysis highlights previously bypassed alternative splice variants, which may be crucial to deciphering more complex pathways of gene regulation in human.
    MeSH term(s) Algorithms ; Alternative Splicing ; Animals ; Base Composition ; Base Sequence ; Conserved Sequence ; Data Mining ; Exons ; Gene Expression Profiling ; Genomics/methods ; Humans ; Introns ; Mice ; Oligonucleotide Array Sequence Analysis ; Protein Isoforms/genetics ; RNA, Messenger/chemistry ; RNA, Untranslated/metabolism ; Sequence Alignment
    Chemical Substances Protein Isoforms ; RNA, Messenger ; RNA, Untranslated
    Language English
    Publishing date 2010-04-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkq197
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

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  4. Article: Unconstrained mining of transcript data reveals increased alternative splicing complexity in the human transcriptome

    Mollet, I.G / Ben-Dov, Claudia / Felício-Silva, Daniel / Grosso, A.R / Eleutério, Pedro / Alves, Ruben / Staller, Ray / Silva, Tito Santos / Carmo-Fonseca, Maria

    Nucleic acids research. 2010 Aug., v. 38, no. 14

    2010  

    Abstract: Mining massive amounts of transcript data for alternative splicing information is paramount to help understand how the maturation of RNA regulates gene expression. We developed an algorithm to cluster transcript data to annotated genes to detect ... ...

    Abstract Mining massive amounts of transcript data for alternative splicing information is paramount to help understand how the maturation of RNA regulates gene expression. We developed an algorithm to cluster transcript data to annotated genes to detect unannotated splice variants. A higher number of alternatively spliced genes and isoforms were found compared to other alternative splicing databases. Comparison of human and mouse data revealed a marked increase, in human, of splice variants incorporating novel exons and retained introns. Previously unannotated exons were validated by tiling array expression data and shown to correspond preferentially to novel first exons. Retained introns were validated by tiling array and deep sequencing data. The majority of retained introns were shorter than 500 nt and had weak polypyrimidine tracts. A subset of retained introns matching small RNAs and displaying a high GC content suggests a possible coordination between splicing regulation and production of noncoding RNAs. Conservation of unannotated exons and retained introns was higher in horse, dog and cow than in rodents, and 64% of exon sequences were only found in primates. This analysis highlights previously bypassed alternative splice variants, which may be crucial to deciphering more complex pathways of gene regulation in human.
    Keywords Primates ; algorithms ; alternative splicing ; cows ; databases ; dogs ; exons ; gene expression ; genes ; genome mining ; high-throughput nucleotide sequencing ; horses ; humans ; introns ; mice ; non-coding RNA ; transcriptome
    Language English
    Dates of publication 2010-08
    Size p. 4740-4754.
    Document type Article
    ZDB-ID 186809-3
    ISSN 0301-5610 ; 0305-1048
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkq197
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 79/704: Show issues

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  5. Article ; Online: Detection of hybridization and species identification in domesticated and wild quails using genetic markers

    Amaral, A.J. / Silva, A.B. / Grosso, A.R. / Chikhi, L. / Bastos-Silveira, C. / Dias, D.

    Folia Zoologica

    2007  Volume 56, Issue 3

    Abstract: Hybridization is particularly widespread in birds and can affect species status and recovery. The common quail Coturnix coturnix is a protected game species that has undergone significant population decrease due to habitat changes. The release of ... ...

    Abstract Hybridization is particularly widespread in birds and can affect species status and recovery. The common quail Coturnix coturnix is a protected game species that has undergone significant population decrease due to habitat changes. The release of Japanese quail C. japonica and or hybrids for restocking has been occurring since the 1970¿s. Both species have not developed reproductive isolating mechanisms and hybridization is occurring. Species distinction based on morphology and male callings is difficult. In this work cytochrome b gene and five microsatellite loci were used with the aim of establishing an identification test for quails sampled in Portugal. Cytochrome b gene revealed to be of promising use to identify the quail maternal lineage. Success in species assignment with the studied microsatellite loci was moderate to identify samples with suspicion of being hybrids with common quail maternal lineage.
    Keywords coturnix-c.-japonica ; dna ; individuals ; mitochondrial genome ; multilocus genotype data ; number ; population-structure ; primers ; simulation ; vertebrates
    Subject code 590
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2108381-2
    ISSN 1573-1189 ; 0139-7893
    ISSN (online) 1573-1189
    ISSN 0139-7893
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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