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  1. Article: Water quality and physical hydrogeology of the Amarapura township, Mandalay, Myanmar

    Grzybowski, Michael / Lenczewski, Melissa E / Oo, Yee Yee

    Hydrogeology journal. 2019 June, v. 27, no. 4

    2019  

    Abstract: Mandalay is a major city in central Myanmar with a high urban population and which lacks a central wastewater management system, a solid waste disposal process, and access to treated drinking water. The purpose of this study is to investigate the ... ...

    Title translation Qualité de l’eau et hydrogéologie physique du bidonville d’Amarapura, Mandalay, au Myanmar Calidad del agua e hidrogeología física del Amarapura township, Mandalay, Myanmar 缅甸曼德勒阿玛拉普拉镇的水质和物理水文地质状况 Qualidade da água e hidrogeologia física da Cidade de Amarapura, Mandalay, Mianmar
    Abstract Mandalay is a major city in central Myanmar with a high urban population and which lacks a central wastewater management system, a solid waste disposal process, and access to treated drinking water. The purpose of this study is to investigate the groundwater quality of local dug wells and tube wells, determine quantitative data on characteristics of the Amarapura Aquifer, and compare seasonal variations in groundwater flow and quality. Water samples were collected during the dry and wet seasons, then analyzed for major ion chemistry using ion chromatography to identify indicators of wastewater contamination transport to the shallow aquifer and to compare seasonal variations in groundwater chemistry. An open-source analytic element model, GFLOW, was used to describe the physical hydrogeology and to determine groundwater flow characteristics in the aquifer. Hydrogeochemistry data and numerical groundwater flow models provide evidence that the Amarapura Aquifer is susceptible to contamination from anthropogenic sources. The dominant water types in most dug wells and tube wells is Na-Cl, but there is no known geologic source of NaCl near Mandalay. Many of these wells also contain water with high electrical conductivity, chloride, nitrate, ammonium, and E. coli. Physical measurements and GFLOW characterize groundwater flow directions predominantly towards the Irrawaddy River and with average linear velocities ranging from 1.76 × 10−2 m/day (2.04 × 10−7 m/s) to 9.25 m/day (1.07 × 10−4 m/s). This is the first hydrogeological characterization conducted in Myanmar.
    Keywords Escherichia coli ; ammonium ; anthropogenic activities ; aquifers ; drinking water ; electrical conductivity ; groundwater ; groundwater flow ; hydrogeochemistry ; ion exchange chromatography ; models ; nitrates ; rivers ; seasonal variation ; sodium chloride ; solid wastes ; urban population ; waste disposal ; wastewater treatment ; water pollution ; water quality ; wells ; wet season ; Myanmar
    Language English
    Dates of publication 2019-06
    Size p. 1497-1513.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 1227482-3
    ISSN 0941-2816 ; 1431-2174
    ISSN 0941-2816 ; 1431-2174
    DOI 10.1007/s10040-018-01922-9
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Chronic stress from adolescence to adulthood increases adiposity and anxiety in rats with decreased expression of

    Szalanczy, Alexandria M / Fitzpatrick, Mackenzie / Beeson, Angela / Bui, Trangdai / Dyson, Christina / Eller, Seth / Landry, Julia / Scott, Christina / Grzybowski, Michael / Klotz, Jason / Geurts, Aron M / Weiner, Jeff L / Redei, Eva E / Solberg Woods, Leah C

    Frontiers in genetics

    2024  Volume 14, Page(s) 1247232

    Abstract: We previously ... ...

    Abstract We previously identified
    Language English
    Publishing date 2024-01-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2023.1247232
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Creation and characterization of novel rat model for recessive dystrophic epidermolysis bullosa: Frameshift mutation of the Col7a1 gene leads to severe blistered phenotype.

    Stone, William / Strege, Chloe / Miller, William / Geurts, Aron M / Grzybowski, Michael / Riddle, Megan / Lees, Christopher / Eide, Cindy / Keene, Douglas R / Tufa, Sara F / Seelig, Davis / McGrath, John / Tolar, Jakub

    PloS one

    2024  Volume 19, Issue 5, Page(s) e0302991

    Abstract: Recessive dystrophic epidermolysis bullosa is a rare genodermatosis caused by a mutation of the Col7a1 gene. The Col7a1 gene codes for collagen type VII protein, a major component of anchoring fibrils. Mutations of the Col7a1 gene can cause aberrant ... ...

    Abstract Recessive dystrophic epidermolysis bullosa is a rare genodermatosis caused by a mutation of the Col7a1 gene. The Col7a1 gene codes for collagen type VII protein, a major component of anchoring fibrils. Mutations of the Col7a1 gene can cause aberrant collagen type VII formation, causing an associated lack or absence of anchoring fibrils. This presents clinically as chronic blistering, scarring, and fibrosis, often leading to the development of cutaneous squamous cell carcinoma. Patients also experience persistent pain and pruritus. Pain management and supportive bandaging remain the primary treatment options. The pathology of recessive dystrophic epidermolysis bullosa was first described in the 1980s, and there has since been a multitude of encouraging treatment options developed. However, in vivo research has been hindered by inadequate models of the disease. The various mouse models in existence possess longevity and surface area constraints, or do not adequately model a normal human disease state. In this paper, we describe a novel rat model of recessive dystrophic epidermolysis bullosa that offers an alternative to previous murine models. An 8-base pair deletion was induced in the Col7a1 gene of Lewis rats, which was subsequently found to cause a premature stop codon downstream. Homozygous mutants presented with a fragile and chronically blistered phenotype postnatally. Further histological analysis revealed subepidermal clefting and the absence of anchoring fibrils. The generation of this novel model offers researchers an easily maintained organism that possesses a larger surface area for experimental topical and transfused therapies to be tested, which may provide great utility in the future study of this debilitating disease.
    MeSH term(s) Collagen Type VII/genetics ; Animals ; Epidermolysis Bullosa Dystrophica/genetics ; Epidermolysis Bullosa Dystrophica/pathology ; Disease Models, Animal ; Rats ; Frameshift Mutation ; Phenotype ; Genes, Recessive ; Rats, Inbred Lew ; Blister/genetics ; Blister/pathology ; Skin/pathology ; Male
    Chemical Substances Collagen Type VII
    Language English
    Publishing date 2024-05-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0302991
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Btg2

    Hoffman, Matthew J / Takizawa, Akiko / Jensen, Eric S / Schilling, Rebecca / Grzybowski, Michael / Geurts, Aron M / Dwinell, Melinda R

    Physiological genomics

    2022  Volume 54, Issue 7, Page(s) 231–241

    Abstract: Hypertension (HTN) is a complex disease influenced by heritable genetic elements and environmental interactions. Dietary salt is among the most influential modifiable factors contributing to increased blood pressure (BP). It is well established that men ... ...

    Abstract Hypertension (HTN) is a complex disease influenced by heritable genetic elements and environmental interactions. Dietary salt is among the most influential modifiable factors contributing to increased blood pressure (BP). It is well established that men and women develop BP impairment in different patterns and a recent emphasis has been placed on identifying mechanisms leading to the differences observed between the sexes in HTN development. The current work reported here builds on an extensive genetic mapping experiment that sought to identify genetic determinants of salt-sensitive (SS) HTN using the Dahl SS rat. BTG antiproliferation factor 2 (
    MeSH term(s) Animals ; Blood Pressure/genetics ; Female ; Humans ; Hypertension/drug therapy ; Immediate-Early Proteins/genetics ; Immediate-Early Proteins/metabolism ; Immediate-Early Proteins/therapeutic use ; Kidney/metabolism ; Mutation/genetics ; Rats ; Rats, Inbred Dahl ; Sodium Chloride, Dietary ; Tumor Suppressor Proteins/genetics ; Tumor Suppressor Proteins/metabolism ; Tumor Suppressor Proteins/therapeutic use
    Chemical Substances BTG2 protein, rat ; Immediate-Early Proteins ; Sodium Chloride, Dietary ; Tumor Suppressor Proteins ; BTG2 protein, human (141490-22-4)
    Language English
    Publishing date 2022-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2038823-8
    ISSN 1531-2267 ; 1094-8341
    ISSN (online) 1531-2267
    ISSN 1094-8341
    DOI 10.1152/physiolgenomics.00167.2021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Changes in Environmental Stress over COVID-19 Pandemic Likely Contributed to Failure to Replicate Adiposity Phenotype Associated with Krtcap3.

    Szalanczy, Alexandria M / Giorgio, Gina / Goff, Emily / Seshie, Osborne / Grzybowski, Michael / Klotz, Jason / Geurts, Aron M / Redei, Eva E / Solberg Woods, Leah C

    bioRxiv : the preprint server for biology

    2023  

    Abstract: We previously identified Keratinocyte-associated protein 3, Krtcap3, as an obesity-related gene in female rats where a whole-body Krtcap3 knock-out (KO) led to increased adiposity compared to wild-type (WT) controls when fed a high-fat diet (HFD). We ... ...

    Abstract We previously identified Keratinocyte-associated protein 3, Krtcap3, as an obesity-related gene in female rats where a whole-body Krtcap3 knock-out (KO) led to increased adiposity compared to wild-type (WT) controls when fed a high-fat diet (HFD). We sought to replicate this work to better understand the function of Krtcap3 but were unable to reproduce the adiposity phenotype. In the current work, WT female rats ate more compared to WT in the prior study, with corresponding increases in body weight and fat mass, while there were no changes in these measures in KO females between the studies. The prior study was conducted before the COVID-19 pandemic, while the current study started after initial lock-down orders and was completed during the pandemic with a generally less stressful environment. We hypothesize that the environmental changes impacted stress levels and may explain the failure to replicate our results. Analysis of corticosterone (CORT) at euthanasia showed a significant study by genotype interaction where WT had significantly higher CORT relative to KO in Study 1, with no differences in Study 2. These data suggest that decreasing Krtcap3 expression may alter the environmental stress response to influence adiposity. We also found that KO rats in both studies, but not WT, experienced a dramatic increase in CORT after their cage mate was removed, suggesting a separate connection to social behavioral stress. Future work is necessary to confirm and elucidate the finer mechanisms of these relationships, but these data indicate the possibility of Krtcap3 as a novel stress gene.
    Language English
    Publishing date 2023-06-25
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.03.15.532439
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: UBASH3A deficiency accelerates type 1 diabetes development and enhances salivary gland inflammation in NOD mice.

    Chen, Yi-Guang / Ciecko, Ashley E / Khaja, Shamim / Grzybowski, Michael / Geurts, Aron M / Lieberman, Scott M

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 12019

    Abstract: Recent advances in genetic analyses have significantly refined human type 1 diabetes (T1D) associated loci. The goal of such effort is to identify the causal genes and have a complete understanding of the molecular pathways that independently or ... ...

    Abstract Recent advances in genetic analyses have significantly refined human type 1 diabetes (T1D) associated loci. The goal of such effort is to identify the causal genes and have a complete understanding of the molecular pathways that independently or interactively influence cellular processes leading to the destruction of insulin producing pancreatic β cells. UBASH3A has been suggested as the underlying gene for a human T1D associated region on chromosome 21. To further evaluate the role of UBASH3A in T1D, we targeted Ubash3a in NOD mice using zinc-finger nuclease mediated mutagenesis. In both 10-week-old females and males, significantly more advanced insulitis was observed in UBASH3A-deficient than in wild-type NOD mice. Consistently, UBASH3A-deficient NOD mice developed accelerated T1D in both sexes, which was associated with increased accumulation of β-cell autoreactive T cells in the spleen and pancreatic lymph node. Adoptive transfer of splenic T cells into NOD.Rag1
    MeSH term(s) Adoptive Transfer ; Animals ; Autoimmunity ; CD4-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/metabolism ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/metabolism ; Diabetes Mellitus, Type 1/genetics ; Diabetes Mellitus, Type 1/immunology ; Diabetes Mellitus, Type 1/metabolism ; Disease Models, Animal ; Female ; Genetic Predisposition to Disease/genetics ; Insulin-Secreting Cells/metabolism ; Male ; Mice ; Mice, Inbred NOD ; Mice, Knockout ; Mutagenesis/drug effects ; Receptors, Antigen, T-Cell/deficiency ; Receptors, Antigen, T-Cell/genetics ; Sialadenitis/metabolism ; Zinc Finger Nucleases/pharmacology
    Chemical Substances Receptors, Antigen, T-Cell ; Sts-2 protein, mouse ; Zinc Finger Nucleases (EC 3.1.-)
    Language English
    Publishing date 2020-07-21
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-68956-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Genetic Disruption of System xc-Mediated Glutamate Release from Astrocytes Increases Negative-Outcome Behaviors While Preserving Basic Brain Function in Rat.

    Hess, Evan M / Kassel, Sara N / Simandl, Gregory / Raddatz, Nicholas / Maunze, Brian / Hurley, Matthew M / Grzybowski, Michael / Klotz, Jason / Geurts, Aron / Liu, Qing-Song / Choi, SuJean / Twining, Robert C / Baker, David A

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2023  Volume 43, Issue 13, Page(s) 2349–2361

    Abstract: The importance of neuronal glutamate to synaptic transmission throughout the brain illustrates the immense therapeutic potential and safety risks of targeting this system. Astrocytes also release glutamate, the clinical relevance of which is unknown as ... ...

    Abstract The importance of neuronal glutamate to synaptic transmission throughout the brain illustrates the immense therapeutic potential and safety risks of targeting this system. Astrocytes also release glutamate, the clinical relevance of which is unknown as the range of brain functions reliant on signaling from these cells hasn't been fully established. Here, we investigated system xc- (Sxc), which is a glutamate release mechanism with an
    MeSH term(s) Rats ; Male ; Animals ; Glutamic Acid/metabolism ; Astrocytes/metabolism ; Synaptic Transmission ; Brain/metabolism ; Neurons/metabolism
    Chemical Substances Glutamic Acid (3KX376GY7L)
    Language English
    Publishing date 2023-02-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.1525-22.2023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Changes in environmental stress over COVID-19 pandemic likely contributed to failure to replicate adiposity phenotype associated with

    Szalanczy, Alexandria M / Giorgio, Gina / Goff, Emily / Seshie, Osborne / Grzybowski, Michael / Klotz, Jason / Geurts, Aron M / Redei, Eva E / Solberg Woods, Leah C

    Physiological genomics

    2023  Volume 55, Issue 10, Page(s) 452–467

    Abstract: We previously identified keratinocyte-associated protein 3, ...

    Abstract We previously identified keratinocyte-associated protein 3,
    MeSH term(s) Humans ; Rats ; Female ; Animals ; Mice ; Adiposity/genetics ; Pandemics ; COVID-19/genetics ; Communicable Disease Control ; Obesity/genetics ; Obesity/metabolism ; Corticosterone ; Diet, High-Fat/adverse effects ; Phenotype ; Mice, Knockout
    Chemical Substances Corticosterone (W980KJ009P)
    Language English
    Publishing date 2023-07-17
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2038823-8
    ISSN 1531-2267 ; 1094-8341
    ISSN (online) 1531-2267
    ISSN 1094-8341
    DOI 10.1152/physiolgenomics.00019.2023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: A "Four Core Genotypes" rat model to distinguish mechanisms underlying sex-biased phenotypes and diseases.

    Arnold, Arthur P / Chen, Xuqi / Grzybowski, Michael N / Ryan, Janelle M / Sengelaub, Dale R / Mohanroy, Tara / Furlan, V Andree / Grisham, William / Malloy, Lynn / Takizawa, Akiko / Wiese, Carrie B / Vergnes, Laurent / Skaletsky, Helen / Page, David C / Reue, Karen / Harley, Vincent R / Dwinell, Melinda R / Geurts, Aron M

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Background: We have generated a rat model similar to the Four Core Genotypes mouse model, allowing comparison of XX and XY rats with the same type of gonad. The model detects novel sex chromosome effects (XX vs. XY) that contribute to sex differences in ...

    Abstract Background: We have generated a rat model similar to the Four Core Genotypes mouse model, allowing comparison of XX and XY rats with the same type of gonad. The model detects novel sex chromosome effects (XX vs. XY) that contribute to sex differences in any rat phenotype.
    Methods: XY rats were produced with an autosomal transgene of
    Results: We have measured numerous phenotypes to characterize this model, including gonadal histology, breeding performance, anogenital distance, levels of reproductive hormones, body and organ weights, and central nervous system sexual dimorphisms. Serum testosterone levels were comparable in adult XX and XY gonadal males. Numerous phenotypes previously found to be sexually differentiated by the action of gonadal hormones were found to be similar in XX and XY rats with the same type of gonad, suggesting that XX and XY rats with the same type of gonad have comparable levels of gonadal hormones at various stages of development.
    Conclusion: The results establish a powerful new model to discriminate sex chromosome and gonadal hormone effects that cause sexual differences in rat physiology and disease.
    Language English
    Publishing date 2023-02-10
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.02.09.527738
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Keratinocyte-associated protein 3

    Szalanczy, Alexandria M / Goff, Emily / Seshie, Osborne / Deal, Aaron / Grzybowski, Michael / Klotz, Jason / Chuang Key, Chia-Chi / Geurts, Aron M / Solberg Woods, Leah C

    Frontiers in genetics

    2022  Volume 13, Page(s) 942574

    Abstract: Despite the obesity crisis in the United States, the underlying genetics are poorly understood. Our lab previously ... ...

    Abstract Despite the obesity crisis in the United States, the underlying genetics are poorly understood. Our lab previously identified
    Language English
    Publishing date 2022-09-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2022.942574
    Database MEDical Literature Analysis and Retrieval System OnLINE

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