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  1. Article ; Online: Efficient production of poly-3-hydroxybutyrate from acetate and butyrate by halophilic bacteria Salinivibrio spp. TGB4 and TGB19.

    Tao, Guan-Bao / Tian, Linyue / Pu, Nan / Li, Zheng-Jun

    International journal of biological macromolecules

    2022  Volume 221, Page(s) 1365–1372

    Abstract: Volatile fatty acids (VFAs) derived from biomass are considered to be economical and environmentally friendly feedstocks for microbial fermentation. Converting VFAs to polyhydroxyalkanoate (PHA) could reduce the substrate cost and provide an economically ...

    Abstract Volatile fatty acids (VFAs) derived from biomass are considered to be economical and environmentally friendly feedstocks for microbial fermentation. Converting VFAs to polyhydroxyalkanoate (PHA) could reduce the substrate cost and provide an economically viable route for the commercialization of PHA. The halophilic bacteria Salinivibrio spp. TGB4 and TGB19, newly isolated from salt fields, were found to accumulate poly-3-hydroxybutyrate (PHB) using acetate or butyrate as the substrate. Both strains exhibited considerable cell growth (OD
    MeSH term(s) Butyrates ; Hydroxybutyrates ; Polyesters/metabolism ; Polyhydroxyalkanoates ; Fatty Acids, Volatile ; Acetates ; Vibrionaceae/metabolism ; Fermentation
    Chemical Substances poly-beta-hydroxybutyrate (26063-00-3) ; Butyrates ; Hydroxybutyrates ; Polyesters ; Polyhydroxyalkanoates ; Fatty Acids, Volatile ; Acetates
    Language English
    Publishing date 2022-09-17
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2022.09.141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Production of polyhydroxyalkanoates by a moderately halophilic bacterium of Salinivibrio sp. TGB10.

    Tao, Guan-Bao / Tan, Bi-Wei / Li, Zheng-Jun

    International journal of biological macromolecules

    2021  Volume 186, Page(s) 574–579

    Abstract: A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84  ...

    Abstract A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84 g/L were obtained using glucose as the carbon source after 24 h of cultivation in shake flasks. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was synthesized when propionate was provided as secondary carbon source. Salinivibrio sp. TGB10 exhibited favorable tolerance to propionate. The use of 8 g/L propionate and 20 g/L glucose as combinational substrates yielded 1.45 g/L PHBV with a 3-hydroxyvalerate monomer content of 72.02 mol% in flask cultures. In bioreactor study, CDW of 33.45 g/L and PHBV titer of 27.36 g/L were obtained after 108 h of fed-batch cultivation. The results indicated that Salinivibrio sp. TGB10 is a promising halophilic bacterium for the production of PHBV with various polymer compositions.
    MeSH term(s) Bioreactors ; Fatty Acids, Volatile/metabolism ; Fermentation ; Hydroxybutyrates/metabolism ; Polyesters/metabolism ; Polyhydroxyalkanoates/metabolism ; Substrate Specificity ; Sugars/metabolism ; Vibrionaceae/enzymology ; Vibrionaceae/growth & development ; Water Microbiology
    Chemical Substances Fatty Acids, Volatile ; Hydroxybutyrates ; Polyesters ; Polyhydroxyalkanoates ; Sugars ; poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) ; poly-beta-hydroxybutyrate (26063-00-3)
    Language English
    Publishing date 2021-07-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2021.07.038
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Hyper production of polyhydroxyalkanoates by a novel bacterium Salinivibrio sp. TGB11

    Tao, Guan-Bao / Pu, Nan / Wang, Meng-Ru / Li, Zheng-Jun

    Biochemical engineering journal. 2022 July, v. 185

    2022  

    Abstract: Strains of Salinivibrio genus are able to grow under wide range of NaCl concentration. Salinivibrio sp. TGB11, a novel bacterium screened from saltworks, was found capable of producing polyhydroxyalkanoates from sugars and volatile fatty acids. In shake ... ...

    Abstract Strains of Salinivibrio genus are able to grow under wide range of NaCl concentration. Salinivibrio sp. TGB11, a novel bacterium screened from saltworks, was found capable of producing polyhydroxyalkanoates from sugars and volatile fatty acids. In shake flask cultures, the use of glucose, sucrose, acetate, and butyrate achieved poly(3-hydroxybutyrate) (PHB) titers of 5.00, 3.23, 0.77, and 0.88 g/L, respectively. The addition of 0.5 g/L propionate with glucose yielded 4.38 g/L poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), with a 3-hydroxyvalerate monomer content of 8.57 mol%, yet propionate concentration higher than 1.5 g/L severely inhibited cell growth. In fed-batch bioreactor studies, PHB titer of 100.22 g/L and PHBV titer of 59.30 g/L were achieved. The PHB production yield was 0.42 g/g, reaching 88% of the theoretical value. Salinivibrio sp. TGB11 showed the highest PHB titer and yield in Salinivibrio genus and could be a promising host for industrial purposes.
    Keywords Salinivibrio ; acetates ; bacteria ; bioreactors ; butyrates ; cell growth ; culture flasks ; glucose ; propionic acid ; sucrose
    Language English
    Dates of publication 2022-07
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2012139-8
    ISSN 1369-703X
    ISSN 1369-703X
    DOI 10.1016/j.bej.2022.108538
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Efficient production of poly-3-hydroxybutyrate from acetate and butyrate by halophilic bacteria Salinivibrio spp. TGB4 and TGB19

    Tao, Guan-Bao / Tian, Linyue / Pu, Nan / Li, Zheng-Jun

    International journal of biological macromolecules. 2022 Nov. 30, v. 221

    2022  

    Abstract: Volatile fatty acids (VFAs) derived from biomass are considered to be economical and environmentally friendly feedstocks for microbial fermentation. Converting VFAs to polyhydroxyalkanoate (PHA) could reduce the substrate cost and provide an economically ...

    Abstract Volatile fatty acids (VFAs) derived from biomass are considered to be economical and environmentally friendly feedstocks for microbial fermentation. Converting VFAs to polyhydroxyalkanoate (PHA) could reduce the substrate cost and provide an economically viable route for the commercialization of PHA. The halophilic bacteria Salinivibrio spp. TGB4 and TGB19, newly isolated from salt fields, were found to accumulate poly-3-hydroxybutyrate (PHB) using acetate or butyrate as the substrate. Both strains exhibited considerable cell growth (OD₆₀₀ of ~8) even at acetate concentration of 100 g/L. In shake flask cultures, TGB4 produced PHB titers of 0.90 and 1.34 g/L, while TGB19 produced PHB titers of 0.25 and 2.53 g/L with acetate and butyrate, respectively. When acetate and butyrate were both applied, PHB production was significantly increased, and the PHB titer of TGB4 and TGB19 reached 6.14 and 6.84 g/L, respectively. After optimizing the culture medium, TGB19 produced 8.42 g/L PHB, corresponding to 88.55 wt% of cell dry weight. During fed-batch cultivation, TGB19 produced a PHB titer of 53.23 g/L. This is the highest reported PHB titer using acetate and butyrate by pure microbial cultures and would provide promising hosts for the industrial production of PHA from VFAs.
    Keywords Salinivibrio ; acetates ; biomass ; butyrates ; cell growth ; commercialization ; culture flasks ; culture media ; economic sustainability ; feedstocks ; fermentation ; poly-3-hydroxybutyrate
    Language English
    Dates of publication 2022-1130
    Size p. 1365-1372.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2022.09.141
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Lactate Dehydrogenase and Risk of Readmission with Gastric Cancer: A Propensity Score Matching Analysis.

    Chen, Wei-Sheng / Huang, Ze-Xin / Zhang, Hui-Hui / Chen, Xiao-Dong / Cai, Yi-Qi / Chen, Wen-Jing / Zhu, Guan-Bao / Huang, Yun-Shi

    Journal of investigative surgery : the official journal of the Academy of Surgical Research

    2023  Volume 36, Issue 1, Page(s) 2172488

    Abstract: Purpose: Readmission is one of the measures of quality of care and potential costs. This study aimed to determine whether lactate dehydrogenase (LDH) is associated with an increased risk of 30-day readmission in gastric cancer.: Methods: We performed ...

    Abstract Purpose: Readmission is one of the measures of quality of care and potential costs. This study aimed to determine whether lactate dehydrogenase (LDH) is associated with an increased risk of 30-day readmission in gastric cancer.
    Methods: We performed a retrospective study of patients who underwent radical gastrectomy for gastric cancer at our institution between July 2014 and May 2018. Balanced cohorts were created by propensity score matching (PSM) with a 1:1 ratio to generate the elevated LDH (ELDH) group (n = 151) and the low LDH group (Control) (n = 302). To determine the incidence, causes, and risk factors of 30-day readmission, subgroup analyzes were performed and used to develop an efficient prediction model.
    Results: A total of 788 patients met the criteria to be included in the study. The cutoff value for serum LDH was 215.5. After PSM, a total of 302 patients were matched in pairs (ELDH group, n = 151, Control group, n = 151). ELDH levels had a higher risk of readmission (p = 0.005, Odds ratio 3.768, 95% confidence interval 1.493-9.510). The pre-match 30-day readmission rate was 7.2 percent, and common causes of post-match readmission included infection-related symptoms, gastrointestinal symptoms, and gastrointestinal bleeding.
    Conclusions: Patients with preoperative ELDH levels, postoperative complications, and high preoperative American Society of Anesthesiologists Scores had a higher risk of readmission 30 days after surgery.
    MeSH term(s) Humans ; Stomach Neoplasms/epidemiology ; Stomach Neoplasms/surgery ; Propensity Score ; Patient Readmission ; Retrospective Studies ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Postoperative Complications/surgery ; Gastrectomy/adverse effects ; Lactate Dehydrogenases
    Chemical Substances Lactate Dehydrogenases (EC 1.1.-)
    Language English
    Publishing date 2023-02-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 639444-9
    ISSN 1521-0553 ; 0894-1939
    ISSN (online) 1521-0553
    ISSN 0894-1939
    DOI 10.1080/08941939.2023.2172488
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Production of polyhydroxyalkanoates by a moderately halophilic bacterium of Salinivibrio sp. TGB10

    Tao, Guan-Bao / Tan, Bi-Wei / Li, Zheng-Jun

    International journal of biological macromolecules. 2021 Sept. 01, v. 186

    2021  

    Abstract: A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84  ...

    Abstract A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84 g/L were obtained using glucose as the carbon source after 24 h of cultivation in shake flasks. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was synthesized when propionate was provided as secondary carbon source. Salinivibrio sp. TGB10 exhibited favorable tolerance to propionate. The use of 8 g/L propionate and 20 g/L glucose as combinational substrates yielded 1.45 g/L PHBV with a 3-hydroxyvalerate monomer content of 72.02 mol% in flask cultures. In bioreactor study, CDW of 33.45 g/L and PHBV titer of 27.36 g/L were obtained after 108 h of fed-batch cultivation. The results indicated that Salinivibrio sp. TGB10 is a promising halophilic bacterium for the production of PHBV with various polymer compositions.
    Keywords Salinivibrio ; bacteria ; bioreactors ; carbon ; glucose ; polyhydroxyalkanoates ; propionic acid
    Language English
    Dates of publication 2021-0901
    Size p. 574-579.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2021.07.038
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: The mechanism of Bushen Huoxue decoction in treating intervertebral disc degeneration based on network pharmacology.

    Feng, Shuai-Hua / Xie, Fang / Yao, Hong-Yan / Wu, Guan-Bao / Sun, Xiang-Yun / Yang, Jie

    Annals of palliative medicine

    2021  Volume 10, Issue 4, Page(s) 3783–3792

    Abstract: Background: This study aimed to explore the mechanism of Bushen Huoxue decoction (BHD) in treating intervertebral disc degeneration using the network pharmacology method.: Methods: Using of oral bioavailability >30% and drug-likeness >0.18 as the ... ...

    Abstract Background: This study aimed to explore the mechanism of Bushen Huoxue decoction (BHD) in treating intervertebral disc degeneration using the network pharmacology method.
    Methods: Using of oral bioavailability >30% and drug-likeness >0.18 as the screening standards, the effective components and targets of BHD were retrieved from the TCMSP database and the BATMAN-TCM database. The disease targets of intervertebral disc degeneration were retrieved from the GeneCards database. The Wayne map of the interaction targets of the effective components of BHD and intervertebral disc degeneration were drawn using R software. The protein-protein interaction (PPI) network of common targets was constructed using STRING software. The network map of the interaction targets of the effective components of BHD-intervertebral disc degeneration was drawn using Cytoscape3.7.2 software. The GO and KEGG enrichment analysis of the common targets of BHD and intervertebral disc degeneration was performed using R software and the related plug-ins to screen the potential pathways and analyze its mechanism.
    Results: This study screened 164 effective components of BHD, 131 interaction targets, 626 targets for degenerative disc disease, and 31 common interaction targets. IL6, VEGFA, CASP3, EGFR, ESR1, and MAPK8 appeared more frequently. These were mainly enriched in the AGE-RAGE, TNF, PI3K Akt, and MAPK signaling pathways.
    Conclusions: BHD mainly intervenes in intervertebral disc degeneration through IL6, VEGFA, CASP3, EGFR, ESR1, and MAPK8. The mechanism of the intervention of BHD on intervertebral disc degeneration may be related to AGE-RAGE, TNF, PI3K Akt, MAPK, and other signal pathways.
    MeSH term(s) Drugs, Chinese Herbal/therapeutic use ; Humans ; Intervertebral Disc Degeneration/drug therapy ; Medicine, Chinese Traditional ; Phosphatidylinositol 3-Kinases
    Chemical Substances Drugs, Chinese Herbal ; bushen huoxue
    Language English
    Publishing date 2021-03-16
    Publishing country China
    Document type Journal Article
    ZDB-ID 2828544-X
    ISSN 2224-5839 ; 2224-5839
    ISSN (online) 2224-5839
    ISSN 2224-5839
    DOI 10.21037/apm-20-2586
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: A laparoscopic radical inguinal lymphadenectomy approach partly preserving great saphenous vein branches can benefit for patients with penile carcinoma.

    Ma, Yongkang / Hao, Jianwei / Yin, Huaqi / Zhu, Mingkai / Guan, Bao / Zhu, Chaoshuai / Dong, Bingqi / Zhao, Shiming / He, Zhaohong / Yang, Tiejun

    BMC surgery

    2022  Volume 22, Issue 1, Page(s) 138

    Abstract: Background: Inguinal lymphadenectomy (iLAD) is effective for penile carcinoma treatment, but usually results in many complications. This study aims to clinically evaluate the feasibility and clinical significance of a laparoscopic radical iLAD approach ... ...

    Abstract Background: Inguinal lymphadenectomy (iLAD) is effective for penile carcinoma treatment, but usually results in many complications. This study aims to clinically evaluate the feasibility and clinical significance of a laparoscopic radical iLAD approach partly preserving great saphenous vein branches for penile carcinoma patients.
    Methods: A total of 48 patients with penile cancer who underwent laparoscopic radical iLAD with retention of the great saphenous vein in Henan Cancer Hospital from 2012 Jan to 2020 Dec were included in this study. Sixteen penile carcinoma patients who underwent laparoscopic radical iLAD preserving parts of superficial branches of the great saphenous vein were identified as the sparing group, and the matched 32 patients who incised those branches were identified as control group. This new procedure was performed by laparoscopy, preserving parts of superficial branches of the great saphenous vein, superficial lateral and medial femoral veins. Clinicopathological features and perioperative variables were recorded. Postoperative complications, including skin flap necrosis, lymphorrhagia, and lower extremity edema were analyzed retrospectively.
    Results: We found that the operative time of the sparing group is significantly longer than the control group (p = 0.011). There was no statistical difference in intraoperative blood loss, the lymph node number per side, average time to remove the drainage tube and postoperative hospital stay between the two groups. Compared to the control group, the sparing group showed a significantly decreased incidence of lower extremity edema (p = 0.018). The preservation of parts of superficial branches of the great saphenous vein was mainly decreased the incidence of edema below ankle (p = 0.034).
    Conclusions: This study demonstrated that the iLAD with preserving parts of superficial branches of the great saphenous vein, with a decreased incidence of postoperative complications, is a safe and feasible approach for penile cancer.
    MeSH term(s) Carcinoma/surgery ; Femoral Vein/pathology ; Humans ; Laparoscopy/methods ; Lymph Node Excision/methods ; Male ; Penile Neoplasms/surgery ; Postoperative Complications/epidemiology ; Retrospective Studies ; Saphenous Vein/pathology ; Saphenous Vein/surgery
    Language English
    Publishing date 2022-04-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2050442-1
    ISSN 1471-2482 ; 1471-2482
    ISSN (online) 1471-2482
    ISSN 1471-2482
    DOI 10.1186/s12893-022-01582-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Effect of Short-Term Preoperative Parenteral Nutrition Support for Gastric Cancer Patients with Sarcopenia: a Propensity Score Matching Analysis.

    Huang, Ze-Xin / Zhang, Hui-Hui / Zhang, Wei-Teng / Shi, Ming-Ming / Ren, Jia-Huan / Xu, Li-Bin / Chen, Xiao-Dong / Zhu, Guan-Bao

    Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract

    2022  Volume 26, Issue 7, Page(s) 1362–1372

    Abstract: Introduction: Sarcopenia is well recognized as an unfavorable prognostic marker for gastric cancer (GC) patients. Currently, few nutritional interventions-such as parenteral nutrition-exist for the treatment of patients with sarcopenia. This study aimed ...

    Abstract Introduction: Sarcopenia is well recognized as an unfavorable prognostic marker for gastric cancer (GC) patients. Currently, few nutritional interventions-such as parenteral nutrition-exist for the treatment of patients with sarcopenia. This study aimed to estimate the effectiveness of short-term preoperative parenteral nutrition (PN) in GC patients with sarcopenia.
    Materials and methods: We collected data on GC patients with sarcopenia who underwent radical gastrectomy at our hospital from 2010 to 2018. A 1:1 ratio propensity score matching (PSM) was applied to establish the PN and control groups. Data were analyzed using the chi-squared, Mann-Whitney U, and Fisher's exact tests.
    Results: In total, 428 patients met the inclusion criteria, and the propensity scores identified 166 matched pairs of patients with sarcopenia. The overall incidence of postoperative complications between both groups was not significantly different (P = 0.728). The PN group had a lower rate of intra-abdominal infection (P = 0.032) and higher hospitalization costs (P < 0.001) than the control group. Multivariate analysis demonstrated that age, Charlson score, and TNM stage were independent risk factors for postoperative complications. Additionally, subgroup analysis revealed that short-term preoperative PN support is associated with decreased postoperative surgical complications in patients with albumin levels < 35 g/L (P = 0.025).
    Conclusion: Short-term preoperative PN support is not associated with reduction of overall complication rate in patients with GC and sarcopenia. However, those with sarcopenia and hypoalbuminemia benefited from preoperative PN support.
    MeSH term(s) Gastrectomy/adverse effects ; Humans ; Parenteral Nutrition ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Postoperative Complications/surgery ; Propensity Score ; Retrospective Studies ; Sarcopenia/complications ; Stomach Neoplasms/complications ; Stomach Neoplasms/surgery
    Language English
    Publishing date 2022-01-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2012365-6
    ISSN 1873-4626 ; 1934-3213 ; 1091-255X
    ISSN (online) 1873-4626 ; 1934-3213
    ISSN 1091-255X
    DOI 10.1007/s11605-021-05185-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Progress in copper-catalyzed trifluoromethylation

    Guan-bao Li / Chao Zhang / Chun Song / Yu-dao Ma

    Beilstein Journal of Organic Chemistry, Vol 14, Iss 1, Pp 155-

    2018  Volume 181

    Abstract: The introduction of trifluoromethyl groups into organic molecules has attracted great attention in the past five years. In this review, we describe the recent efforts in the development of trifluoromethylation via copper catalysis using nucleophilic, ... ...

    Abstract The introduction of trifluoromethyl groups into organic molecules has attracted great attention in the past five years. In this review, we describe the recent efforts in the development of trifluoromethylation via copper catalysis using nucleophilic, electrophilic or radical trifluoromethylation reagents.
    Keywords copper ; fluorine ; trifluoromethylation ; Science ; Q ; Organic chemistry ; QD241-441
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Beilstein-Institut
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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