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  1. Article ; Online: Intranasal delivery of LaAg vaccine improves immunity of aged mice against visceral Leishmaniasis.

    Salgado, Caio Loureiro / Corea, Andrés Felipe Mendéz / Covre, Luciana Polaco / Fonseca-Martins, Alessandra Marcia da / Falqueto, Aloisio / Guedes, Herbert Leonel de Matos / Rossi-Bergmann, Bartira / Gomes, Daniel Cláudio Oliviera

    Acta tropica

    2024  Volume 252, Page(s) 107125

    Abstract: There are no approved vaccines yet for human visceral leishmaniasis (VL), the most severe form of the leishmaniasis clinical manifestations that is fatal in over 95 % of untreated cases. It is well-accepted that immunological changes during aging have ... ...

    Abstract There are no approved vaccines yet for human visceral leishmaniasis (VL), the most severe form of the leishmaniasis clinical manifestations that is fatal in over 95 % of untreated cases. It is well-accepted that immunological changes during aging have deleterious impact on the efficacy of vaccines and response to infections. In this work, we compared the response of young and aged mice to intranasal vaccination with killed Leishmania amazonensis promastigote antigens (LaAg) that were then challenged with L. infantum infection, a species that causes visceral leishmaniasis. Intranasal vaccination with LaAg induced a similar reduction in parasitism and hepatosplenomegaly in both young and aged mice compared to their unvaccinated counterparts. Following infection, there was also a less prominent inflammatory profile particularly in the vaccinated aged group, with lower production of TNF-α and nitrite compared to the respective unvaccinated group. Interestingly, the LaAg intranasal vaccination promoted increased production of IFN-γ that was observed in both young- and aged vaccinated groups. Additionally, CD4
    MeSH term(s) Humans ; Animals ; Mice ; Aged ; Leishmaniasis, Visceral/prevention & control ; Programmed Cell Death 1 Receptor ; Antigens, Protozoan ; Leishmania mexicana ; Leishmaniasis ; Protozoan Vaccines ; Mice, Inbred BALB C ; Leishmaniasis Vaccines ; Leishmania infantum ; Cytokines
    Chemical Substances Programmed Cell Death 1 Receptor ; Antigens, Protozoan ; Protozoan Vaccines ; Leishmaniasis Vaccines ; Cytokines
    Language English
    Publishing date 2024-01-26
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2024.107125
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.136: Show issues Location:
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  2. Article ; Online: Transcriptomic landscape of skin lesions in cutaneous leishmaniasis reveals a strong CD8

    Fantecelle, Carlos Henrique / Covre, Luciana Polaco / Garcia de Moura, Renan / Guedes, Herbert Leonel de Matos / Amorim, Camila Farias / Scott, Phillip / Mosser, David / Falqueto, Aloisio / Akbar, Arne N / Gomes, Daniel Claudio Oliveira

    Immunology

    2021  Volume 164, Issue 4, Page(s) 754–765

    Abstract: The severity of lesions that develop in patients infected by Leishmania braziliensis is mainly associated with a highly cytotoxic and inflammatory cutaneous environment. Recently, we demonstrated that senescent T and NK cells play a role in the ... ...

    Abstract The severity of lesions that develop in patients infected by Leishmania braziliensis is mainly associated with a highly cytotoxic and inflammatory cutaneous environment. Recently, we demonstrated that senescent T and NK cells play a role in the establishment and maintenance of this tissue inflammation. Here, we extended those findings using transcriptomic analyses that demonstrate a strong co-induction of senescence and pro-inflammatory gene signatures in cutaneous leishmaniasis (CL) lesions. The senescence-associated signature was characterized by marked expression of key genes such as ATM, Sestrin 2, p16, p21 and p38. The cell type identification from deconvolution of bulk sequencing data showed that the senescence signature was linked with CD8
    MeSH term(s) Biomarkers ; Biopsy ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/metabolism ; Computational Biology/methods ; Cytokines/genetics ; Cytokines/metabolism ; Databases, Genetic ; Disease Susceptibility/immunology ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Immunosenescence/genetics ; Inflammation Mediators/metabolism ; Leishmania braziliensis/immunology ; Leishmaniasis, Cutaneous/etiology ; Leishmaniasis, Cutaneous/metabolism ; Leishmaniasis, Cutaneous/pathology ; Parasite Load ; Skin/pathology ; Transcriptome
    Chemical Substances Biomarkers ; Cytokines ; Inflammation Mediators
    Language English
    Publishing date 2021-09-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80124-0
    ISSN 1365-2567 ; 0019-2805 ; 0953-4954
    ISSN (online) 1365-2567
    ISSN 0019-2805 ; 0953-4954
    DOI 10.1111/imm.13410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.181: Show issues Location:
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  3. Article ; Online: Compartmentalized cytotoxic immune response leads to distinct pathogenic roles of natural killer and senescent CD8

    Covre, Luciana Polaco / Devine, Oliver Patrick / Garcia de Moura, Renan / Vukmanovic-Stejic, Milica / Dietze, Reynaldo / Ribeiro-Rodrigues, Rodrigo / Guedes, Herbert Leonel de Matos / Lubiana Zanotti, Raphael / Falqueto, Aloisio / Akbar, Arne N / Gomes, Daniel Claudio Oliveira

    Immunology

    2020  Volume 159, Issue 4, Page(s) 429–440

    Abstract: Cytotoxic activity mediated by ... ...

    Abstract Cytotoxic activity mediated by CD8
    MeSH term(s) CD56 Antigen/genetics ; CD56 Antigen/immunology ; CD57 Antigens/genetics ; CD57 Antigens/immunology ; Case-Control Studies ; Cellular Senescence/immunology ; Cytotoxicity, Immunologic ; Female ; Gene Expression Regulation ; Host-Parasite Interactions/genetics ; Host-Parasite Interactions/immunology ; Humans ; Interferon-gamma/genetics ; Interferon-gamma/immunology ; Killer Cells, Natural/immunology ; Killer Cells, Natural/parasitology ; Killer Cells, Natural/pathology ; Lectins, C-Type/genetics ; Lectins, C-Type/immunology ; Leishmania braziliensis/immunology ; Leishmania braziliensis/pathogenicity ; Leishmaniasis, Cutaneous/immunology ; Leishmaniasis, Cutaneous/parasitology ; Leishmaniasis, Cutaneous/pathology ; Male ; Oligosaccharides/genetics ; Oligosaccharides/immunology ; Receptors, Immunologic/genetics ; Receptors, Immunologic/immunology ; Severity of Illness Index ; Sialyl Lewis X Antigen/analogs & derivatives ; Sialyl Lewis X Antigen/genetics ; Sialyl Lewis X Antigen/immunology ; Signal Transduction ; Skin/immunology ; Skin/parasitology ; Skin/pathology ; T-Lymphocytes, Cytotoxic/immunology ; T-Lymphocytes, Cytotoxic/parasitology ; T-Lymphocytes, Cytotoxic/pathology
    Chemical Substances 6-sulfo sialyl Lewis X ; CD56 Antigen ; CD57 Antigens ; KLRG1 protein, human ; Lectins, C-Type ; NCAM1 protein, human ; Oligosaccharides ; Receptors, Immunologic ; Sialyl Lewis X Antigen ; Interferon-gamma (82115-62-6)
    Language English
    Publishing date 2020-01-26
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80124-0
    ISSN 1365-2567 ; 0019-2805 ; 0953-4954
    ISSN (online) 1365-2567
    ISSN 0019-2805 ; 0953-4954
    DOI 10.1111/imm.13173
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.181: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Intranasal vaccination with killed Leishmania amazonensis promastigotes antigen (LaAg) associated with CAF01 adjuvant induces partial protection in BALB/c mice challenged with Leishmania (infantum) chagasi.

    Leal, Janine Miranda / Mosquini, Marcelle / Covre, Luciana Polaco / Stagmiller, Nataly Pescinalli / Rodrigues, Rodrigo Ribeiro / Christensen, Dennis / de Matos Guedes, Herbert Leonel / Guedes, Herbert Leonel De Matos / Rossi-Bergmann, Bartira / Gomes, Daniel Cláudio De Oliviera

    Parasitology

    2015  Volume 142, Issue 13, Page(s) 1640–1646

    Abstract: The CAF01 adjuvant has previously been shown to be safe for human use and to be a potent adjuvant for several vaccine antigens. In the present work, we sought to optimize the Leishmania amazonensis antigens (LaAg) intranasal vaccine in an attempt to ... ...

    Abstract The CAF01 adjuvant has previously been shown to be safe for human use and to be a potent adjuvant for several vaccine antigens. In the present work, we sought to optimize the Leishmania amazonensis antigens (LaAg) intranasal vaccine in an attempt to enhance the protective immune responses against Leishmania (infantum) chagasi by using the CAF01 association. LaAg/CAF01 vaccinated mice that were challenged 15 days after booster dose with L. (infantum) chagasi showed a significant reduction in their parasite burden in both the spleen and liver, which is associated with an increase in specific production of IFN-γ and nitrite, and a decrease in IL-4 production. In addition, LaAg/CAF01 intranasal delivery was able to increase lymphoproliferative immune responses after parasite antigen recall. These results suggest the feasibility of using the intranasal route for the delivery of crude antigens and of a human-compatible adjuvant against visceral leishmaniasis.
    MeSH term(s) Adjuvants, Immunologic/administration & dosage ; Administration, Intranasal ; Animals ; Antibodies, Protozoan/blood ; Antigens, Protozoan/immunology ; Cytokines/biosynthesis ; Epitopes ; Female ; Immunity, Cellular ; Immunoglobulin G/blood ; Leishmania infantum/immunology ; Leishmania mexicana/immunology ; Leishmaniasis, Visceral/prevention & control ; Liver/parasitology ; Mice ; Mice, Inbred BALB C ; Nitrites/metabolism ; Protozoan Vaccines/administration & dosage ; Spleen/cytology ; Spleen/immunology ; Spleen/parasitology ; Vaccines, Inactivated/administration & dosage
    Chemical Substances Adjuvants, Immunologic ; Antibodies, Protozoan ; Antigens, Protozoan ; Cytokines ; Epitopes ; Immunoglobulin G ; Nitrites ; Protozoan Vaccines ; Vaccines, Inactivated
    Language English
    Publishing date 2015-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 207627-5
    ISSN 1469-8161 ; 0031-1820
    ISSN (online) 1469-8161
    ISSN 0031-1820
    DOI 10.1017/S0031182015001250
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uh II Zs.220: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Intranasal vaccination with killed Leishmania amazonensis promastigotes antigen (LaAg) associated with CAF01 adjuvant induces partial protection in BALB/c mice challenged with Leishmania (infantum) chagasi

    LEAL, JANINE MIRANDA / MOSQUINI, MARCELLE / COVRE, LUCIANA POLACO / STAGMILLER, NATALY PESCINALLI / RODRIGUES, RODRIGO RIBEIRO / CHRISTENSEN, DENNIS / GUEDES, HERBERT LEONEL DE MATOS / ROSSI-BERGMANN, BARTIRA / GOMES, DANIEL CLÁUDIO DE OLIVIERA

    Parasitology. 2015 Nov., v. 142, no. 13

    2015  

    Abstract: The CAF01 adjuvant has previously been shown to be safe for human use and to be a potent adjuvant for several vaccine antigens. In the present work, we sought to optimize the Leishmania amazonensis antigens (LaAg) intranasal vaccine in an attempt to ... ...

    Abstract The CAF01 adjuvant has previously been shown to be safe for human use and to be a potent adjuvant for several vaccine antigens. In the present work, we sought to optimize the Leishmania amazonensis antigens (LaAg) intranasal vaccine in an attempt to enhance the protective immune responses against Leishmania (infantum) chagasi by using the CAF01 association. LaAg/CAF01 vaccinated mice that were challenged 15 days after booster dose with L. (infantum) chagasi showed a significant reduction in their parasite burden in both the spleen and liver, which is associated with an increase in specific production of IFN-γ and nitrite, and a decrease in IL-4 production. In addition, LaAg/CAF01 intranasal delivery was able to increase lymphoproliferative immune responses after parasite antigen recall. These results suggest the feasibility of using the intranasal route for the delivery of crude antigens and of a human-compatible adjuvant against visceral leishmaniasis.
    Keywords Leishmania amazonensis ; adjuvants ; antigens ; immune response ; interferon-gamma ; interleukin-4 ; liver ; mice ; nitrites ; parasites ; promastigotes ; spleen ; vaccination ; vaccines ; visceral leishmaniasis
    Language English
    Dates of publication 2015-11
    Size p. 1640-1646.
    Publishing place Cambridge University Press
    Document type Article
    ZDB-ID 207627-5
    ISSN 1469-8161 ; 0031-1820
    ISSN (online) 1469-8161
    ISSN 0031-1820
    DOI 10.1017/S0031182015001250
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4' 55/15: Show issues

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  6. Article: PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen.

    Guedes, Herbert Leonel de Matos / Guimarães, Allan Jefferson / Muniz, Mauro de Medeiros / Pizzini, Claudia Vera / Hamilton, Andrew John / Peralta, José Mauro / Deepe, George S / Zancopé-Oliveira, Rosely M

    Journal of clinical microbiology

    2002  Volume 41, Issue 2, Page(s) 535–539

    Abstract: The major diagnostic antigens of Histoplasma capsulatum var. capsulatum are the H and M antigens, pluripotent glycoproteins that elicit both humoral and T-cell-mediated immune responses. The gene encoding the M antigen has previously been sequenced, and ... ...

    Abstract The major diagnostic antigens of Histoplasma capsulatum var. capsulatum are the H and M antigens, pluripotent glycoproteins that elicit both humoral and T-cell-mediated immune responses. The gene encoding the M antigen has previously been sequenced, and its sequence has significant overall homology to those of the genes for fungal catalases. Regions of the M-antigen gene with little or no homology were used to design four oligonucleotide sequences for application in the PCR detection and identification of H. capsulatum var. capsulatum. The PCR correctly identified the 31 H. capsulatum var. capsulatum strains isolated from human, animal, and soil specimens and 1 H. capsulatum var. duboisii isolate. PCR products of 111 and 279 bp were amplified with primers Msp1F-Msp1R and Msp2F-Msp2R, respectively. No amplification product was obtained from DNA extracted from an H. capsulatum var. farciminosum isolate. The specificity of the PCR with the M-antigen-derived primers was confirmed by the total absence of amplification products when genomic DNA from Paracoccidioides brasiliensis, Candida spp., Sporothrix schenckii, Cryptococcus neoformans, Blastomyces dermatitidis, Coccidioides immitis, Aspergillus niger, and Aspergillus fumigatus were applied in the reaction. This rapid, sensitive, and specific assay provides a way to identify typical and atypical isolates of H. capsulatum var. capsulatum.
    MeSH term(s) Antigens, Fungal/genetics ; DNA, Fungal/analysis ; Glycoproteins/genetics ; Histoplasma/genetics ; Histoplasma/isolation & purification ; Humans ; Polymerase Chain Reaction/methods
    Chemical Substances Antigens, Fungal ; DNA, Fungal ; Glycoproteins ; M antigen, Ajellomyces capsulatus
    Language English
    Publishing date 2002-08-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/JCM.41.2.535-539.2003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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