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Article ; Online: Platelet Activation and Severe Bleeding During Extracorporeal Carbon Dioxide Removal in Chronic Obstructive Pulmonary Disease Patients.

Smadja, David M / Chocron, Richard / Rivet, Nadia / Ortuno, Sofia / Guerin, Coralie L / Diehl, Jean-Luc

ASAIO journal (American Society for Artificial Internal Organs : 1992)

2024

Language	English
Publishing date	2024-05-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	759982-1
ISSN	1538-943X ; 0162-1432 ; 1058-2916
ISSN (online)	1538-943X
ISSN	0162-1432 ; 1058-2916
DOI	10.1097/MAT.0000000000002241
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Uk I Zs.199: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.B 2435: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)

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Article ; Online: The Onset of Intussusceptive Angiogenesis in COVID-19 Patients Might Come from the Mobilization of Stem Cell Sub-Populations Expressing the Hemangioblast Marker CD143.

Soret, Lou / Guerin, Coralie L / Goudot, Guillaume / Guyonnet, Léa / Diehl, Jean-Luc / Philippe, Aurélien / Gaussem, Pascale / Smadja, David M

Stem cell reviews and reports

2024

Abstract: COVID-19 and infectious diseases have been included in strategic development goals (SDG) of United Nations (UN). The SARS-CoV-2 pandemic has unveiled complex pathophysiological mechanisms underpinning COVID-19, notably inducing a systemic acquired ... ...

Abstract	COVID-19 and infectious diseases have been included in strategic development goals (SDG) of United Nations (UN). The SARS-CoV-2 pandemic has unveiled complex pathophysiological mechanisms underpinning COVID-19, notably inducing a systemic acquired vascular hemopathy characterized by endothelial dysfunction and intussusceptive angiogenesis, a rapid vascular remodeling process identified as a hallmark in severe COVID-19 cases affecting pulmonary and cardiac tissues. Stem cell migration have been proposed as significant regulators of this neoangiogenic process. In a monocentric cross-sectional study, through spectral flow cytometry analysis of peripheral blood mononuclear cells, we identified a distinct stem cell subpopulation mobilized in critical COVID-19. Indeed, by an unsupervised analysis generating a UMAP representation we highlighted eleven different clusters in critical and non-critical COVID-19 patients. Only one cluster was significantly associated to critical COVID-19 compared to non-critical patients. This cluster expressed the markers: CD45dim, CD34+, CD117+, CD147+, and CD143+, and were negative for CD133. Higher level of expression of hemangioblast markers CD143 were found in critical COVID-19 patients. This population, indicative of hemangioblast-like cells, suggests a key role in COVID-19-related neoangiogenesis, potentially driving the severe vascular complications observed. Our findings underscore the need for further investigation into the contributions of adult stem cells in COVID-19 pathology, offering new insights into therapeutic targets and interventions.
Language	English
Publishing date	2024-05-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	2495577-2
ISSN	2629-3277 ; 1558-6804 ; 1550-8943
ISSN (online)	2629-3277 ; 1558-6804
ISSN	1550-8943
DOI	10.1007/s12015-024-10727-1
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Zs.A 6198: Show issues

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Article ; Online: Results of an international survey about methods used to isolate human endothelial colony-forming cells: guidance from the SSC on Vascular Biology of the ISTH.

Blandinières, Adeline / Randi, Anna M / Paschalaki, Koralia E / Guerin, Coralie L / Melero-Martin, Juan M / Smadja, David M

Journal of thrombosis and haemostasis : JTH

2023 Volume 21, Issue 9, Page(s) 2611–2619

Abstract: Background: Assessment of endothelial colony-forming cell (ECFC) number and vasculogenic properties is crucial for exploring vascular diseases and regeneration strategies. A previous survey of the Scientific and Standardization Committee on Vascular ... ...

Abstract	Background: Assessment of endothelial colony-forming cell (ECFC) number and vasculogenic properties is crucial for exploring vascular diseases and regeneration strategies. A previous survey of the Scientific and Standardization Committee on Vascular Biology of the International Society on Thrombosis and Haemostasis clarified key methodological points but highlighted a lack of standardization associated with ECFC culture. Objectives: The aim of this study was to provide expert consensus guidance on ECFC isolation and culture. Methods: We surveyed 21 experts from 10 different countries using a questionnaire proposed during the 2019 International Society on Thrombosis and Haemostasis Congress in Melbourne (Australia) to attain a consensus on ECFC isolation and culture. Results: We report here the consolidated results of the questionnaire. There was agreement on several general statements, mainly the technical aspects of ECFC isolation and cell culture. In contrast, on the points concerning the definition of a colony of ECFCs, the quantification of ECFCs, and the estimation of their age (in days or number of passages), the expert opinions were widely dispersed. Conclusion: Our survey clearly indicates an unmet need for rigorous standardization, multicenter comparison of results, and validation of ECFC isolation and culture procedures for clinical laboratory practice and robustness of results. To this end, we propose a standardized protocol for the isolation and expansion of ECFCs from umbilical cord and adult peripheral blood.
MeSH term(s)	Adult ; Humans ; Endothelial Cells ; Cell Culture Techniques ; Biology ; Australia ; Cells, Cultured ; Neovascularization, Physiologic
Language	English
Publishing date	2023-06-17
Publishing country	England
Document type	Multicenter Study ; Journal Article
ZDB-ID	2112661-6
ISSN	1538-7836 ; 1538-7933
ISSN (online)	1538-7836
ISSN	1538-7933
DOI	10.1016/j.jtha.2023.06.014
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Zs.A 5805: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Do Endothelial Colony-forming Cells Come From Bone Marrow or Vessels/VSELs?

Detriche, Grégoire / Guerin, Coralie L / Gendron, Nicolas / Mirault, Tristan / Smadja, David M

Stem cell reviews and reports

2021 Volume 17, Issue 4, Page(s) 1500–1502

MeSH term(s)	Blood Vessels/cytology ; Bone Marrow ; Embryonic Stem Cells ; Endothelial Cells ; Pluripotent Stem Cells
Language	English
Publishing date	2021-03-02
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	2495577-2
ISSN	2629-3277 ; 1558-6804 ; 1550-8943
ISSN (online)	2629-3277 ; 1558-6804
ISSN	1550-8943
DOI	10.1007/s12015-021-10140-y
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Zs.A 6198: Show issues

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Article ; Online: Tunable DNMT1 degradation reveals DNMT1/DNMT3B synergy in DNA methylation and genome organization.

Scelfo, Andrea / Barra, Viviana / Abdennur, Nezar / Spracklin, George / Busato, Florence / Salinas-Luypaert, Catalina / Bonaiti, Elena / Velasco, Guillaume / Bonhomme, Frédéric / Chipont, Anna / Tijhuis, Andréa E / Spierings, Diana C J / Guérin, Coralie / Arimondo, Paola / Francastel, Claire / Foijer, Floris / Tost, Jӧrg / Mirny, Leonid / Fachinetti, Daniele

The Journal of cell biology

2024 Volume 223, Issue 4

Abstract: DNA methylation (DNAme) is a key epigenetic mark that regulates critical biological processes maintaining overall genome stability. Given its pleiotropic function, studies of DNAme dynamics are crucial, but currently available tools to interfere with ... ...

Abstract	DNA methylation (DNAme) is a key epigenetic mark that regulates critical biological processes maintaining overall genome stability. Given its pleiotropic function, studies of DNAme dynamics are crucial, but currently available tools to interfere with DNAme have limitations and major cytotoxic side effects. Here, we present cell models that allow inducible and reversible DNAme modulation through DNMT1 depletion. By dynamically assessing whole genome and locus-specific effects of induced passive demethylation through cell divisions, we reveal a cooperative activity between DNMT1 and DNMT3B, but not of DNMT3A, to maintain and control DNAme. We show that gradual loss of DNAme is accompanied by progressive and reversible changes in heterochromatin, compartmentalization, and peripheral localization. DNA methylation loss coincides with a gradual reduction of cell fitness due to G1 arrest, with minor levels of mitotic failure. Altogether, this system allows DNMTs and DNA methylation studies with fine temporal resolution, which may help to reveal the etiologic link between DNAme dysfunction and human disease.
MeSH term(s)	Humans ; Cell Division ; DNA Methylation ; Epigenomics ; Heterochromatin/genetics ; DNA (Cytosine-5-)-Methyltransferase 1/genetics ; DNA Methyltransferase 3A/genetics ; Cell Line
Chemical Substances	Heterochromatin ; DNMT1 protein, human (EC 2.1.1.37) ; DNMT3A protein, human ; DNA (Cytosine-5-)-Methyltransferase 1 (EC 2.1.1.37) ; DNA Methyltransferase 3A (EC 2.1.1.37)
Language	English
Publishing date	2024-02-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218154-x
ISSN	1540-8140 ; 0021-9525
ISSN (online)	1540-8140
ISSN	0021-9525
DOI	10.1083/jcb.202307026
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Ub I Zs.190: Show issues

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Article ; Online: Detection of the interactions of tumour derived extracellular vesicles with immune cells is dependent on EV-labelling methods.

Loconte, Luisa / Arguedas, Davinia / El, Rojbin / Zhou, Alix / Chipont, Anna / Guyonnet, Lea / Guerin, Coralie / Piovesana, Ester / Vázquez-Ibar, José Luis / Joliot, Alain / Théry, Clotilde / Martín-Jaular, Lorena

Journal of extracellular vesicles

2023 Volume 12, Issue 12, Page(s) e12384

Abstract: Cell-cell communication within the complex tumour microenvironment is critical to cancer progression. Tumor-derived extracellular vesicles (TD-EVs) are key players in this process. They can interact with immune cells and modulate their activity, either ... ...

Abstract	Cell-cell communication within the complex tumour microenvironment is critical to cancer progression. Tumor-derived extracellular vesicles (TD-EVs) are key players in this process. They can interact with immune cells and modulate their activity, either suppressing or activating the immune system. Deciphering the interactions between TD-EVs and immune cells is essential to understand immune modulation by cancer cells. Fluorescent labelling of TD-EVs is a method of choice to study such interaction. This work aims to determine the impact of EV labelling methods on the detection by imaging flow cytometry and multicolour spectral flow cytometry of EV interaction and capture by the different immune cell types within human Peripheral Blood Mononuclear Cells (PBMCs). EVs released by the triple-negative breast carcinoma cell line MDA-MB-231 were labelled either with the lipophilic dye MemGlow-488 (MG-488), Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) or through ectopic expression of a MyrPalm-superFolderGFP reporter (mp-sfGFP), which incorporates into EVs during their biogenesis. Our results show that these labelling strategies, although analysed with the same techniques, led to diverging results. While MG-488-labelled EVs incorporate in all cell types, CFSE-labelled EVs are restricted to a minor subset of cells and mp-sfGFP-labelled EVs are mainly detected in CD14+ monocytes which are the main uptakers of EVs and other particles, regardless of the labelling method. Furthermore, our results show that the method used for EV labelling influences the detection of the different types of EV interactions with the recipient cells. Specifically, MG-488, CFSE and mp-sfGFP result in observation suggesting, respectively, transient EV-PM interaction that results in dye transfer, EV content delivery, and capture of intact EVs. Consequently, the type of EV labelling method has to be considered as they can provide complementary information on various types of EV-cell interaction and EV fate.
MeSH term(s)	Humans ; Extracellular Vesicles/metabolism ; Leukocytes, Mononuclear ; Succinimides/metabolism ; Cell Line
Chemical Substances	5-(6)-carboxyfluorescein diacetate succinimidyl ester ; Succinimides
Language	English
Publishing date	2023-01-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2683797-3
ISSN	2001-3078 ; 2001-3078
ISSN (online)	2001-3078
ISSN	2001-3078
DOI	10.1002/jev2.12384
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Article ; Online: Severity of endothelial dysfunction is associated with the occurrence of hemorrhagic complications in COPD patients treated by extracorporeal CO

Diehl, Jean-Luc / Augy, Jean Loup / Rivet, Nadia / Guerin, Coralie / Chocron, Richard / Smadja, David M

Intensive care medicine

2020 Volume 46, Issue 10, Page(s) 1950–1952

MeSH term(s)	Carbon Dioxide ; Extracorporeal Membrane Oxygenation ; Humans ; Pulmonary Disease, Chronic Obstructive/complications ; Vascular Diseases
Chemical Substances	Carbon Dioxide (142M471B3J)
Language	English
Publishing date	2020-06-09
Publishing country	United States
Document type	Letter
ZDB-ID	80387-x
ISSN	1432-1238 ; 0340-0964 ; 0342-4642 ; 0935-1701
ISSN (online)	1432-1238
ISSN	0340-0964 ; 0342-4642 ; 0935-1701
DOI	10.1007/s00134-020-06138-8
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Zs.A 1089: Show issues

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Article ; Online: Residual ANTXR1+ myofibroblasts after chemotherapy inhibit anti-tumor immunity via YAP1 signaling pathway.

Licaj, Monika / Mhaidly, Rana / Kieffer, Yann / Croizer, Hugo / Bonneau, Claire / Meng, Arnaud / Djerroudi, Lounes / Mujangi-Ebeka, Kevin / Hocine, Hocine R / Bourachot, Brigitte / Magagna, Ilaria / Leclere, Renaud / Guyonnet, Lea / Bohec, Mylene / Guérin, Coralie / Baulande, Sylvain / Kamal, Maud / Le Tourneau, Christophe / Lecuru, Fabrice /

Becette, Véronique / Rouzier, Roman / Vincent-Salomon, Anne / Gentric, Geraldine / Mechta-Grigoriou, Fatima

Nature communications

2024 Volume 15, Issue 1, Page(s) 1312

Abstract: Although cancer-associated fibroblast (CAF) heterogeneity is well-established, the impact of chemotherapy on CAF populations remains poorly understood. Here we address this question in high-grade serous ovarian cancer (HGSOC), in which we previously ... ...

Abstract	Although cancer-associated fibroblast (CAF) heterogeneity is well-established, the impact of chemotherapy on CAF populations remains poorly understood. Here we address this question in high-grade serous ovarian cancer (HGSOC), in which we previously identified 4 CAF populations. While the global content in stroma increases in HGSOC after chemotherapy, the proportion of FAP
MeSH term(s)	Female ; Humans ; Cancer-Associated Fibroblasts/metabolism ; Microfilament Proteins/metabolism ; Myofibroblasts/metabolism ; Ovarian Neoplasms/pathology ; Ovary/metabolism ; Receptors, Cell Surface/metabolism ; Signal Transduction ; Tumor Microenvironment
Chemical Substances	ANTXR1 protein, human ; Microfilament Proteins ; Receptors, Cell Surface
Language	English
Publishing date	2024-02-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45595-3
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Article: Low-Intensity Pulsed Ultrasound-Mediated Blood-Brain Barrier Opening Increases Anti-Programmed Death-Ligand 1 Delivery and Efficacy in Gl261 Mouse Model.

Ahmed, Mohammed H / Hernández-Verdin, Isaias / Quissac, Emie / Lemaire, Nolwenn / Guerin, Coralie / Guyonnet, Lea / Zahr, Noël / Mouton, Laura / Santin, Mathieu / Petiet, Alexandra / Schmitt, Charlotte / Bouchoux, Guillaume / Canney, Michael / Sanson, Marc / Verreault, Maïté / Carpentier, Alexandre / Idbaih, Ahmed

Pharmaceutics

2023 Volume 15, Issue 2

Abstract: Therapeutic antibodies targeting immune checkpoints have shown limited efficacy in clinical trials in glioblastoma (GBM) patients. Ultrasound-mediated blood-brain barrier opening (UMBO) using low-intensity pulsed ultrasound improved drug delivery to the ... ...

Abstract	Therapeutic antibodies targeting immune checkpoints have shown limited efficacy in clinical trials in glioblastoma (GBM) patients. Ultrasound-mediated blood-brain barrier opening (UMBO) using low-intensity pulsed ultrasound improved drug delivery to the brain. We explored the safety and the efficacy of UMBO plus immune checkpoint inhibitors in preclinical models of GBM. A blood-brain barrier (BBB) opening was performed using a 1 MHz preclinical ultrasound system in combination with 10 µL/g microbubbles. Brain penetration of immune checkpoint inhibitors was determined, and immune cell populations were evaluated using flow cytometry. The impact of repeated treatments on survival was determined. In syngeneic GL261-bearing immunocompetent mice, we showed that UMBO safely and repeatedly opened the BBB. BBB opening was confirmed visually and microscopically using Evans blue dye and magnetic resonance imaging. UMBO plus anti-PDL-1 was associated with a significant improvement of overall survival compared to anti-PD-L1 alone. Using mass spectroscopy, we showed that the penetration of therapeutic antibodies can be increased when delivered intravenously compared to non-sonicated brains. Furthermore, we observed an enhancement of activated microglia percentage when combined with anti-PD-L1. Here, we report that the combination of UMBO and anti-PD-L1 dramatically increases GL261-bearing mice's survival compared to their counterparts treated with anti-PD-L1 alone. Our study highlights the BBB as a limitation to overcome in order to increase the efficacy of anti-PD-L1 in GBM and supports clinical trials combining UMBO and in GBM patients.
Language	English
Publishing date	2023-01-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics15020455
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Article ; Online: Phenotyping polarization dynamics of immune cells using a lipid droplet-cell pairing microfluidic platform.

Pinon, Léa / Ruyssen, Nicolas / Pineau, Judith / Mesdjian, Olivier / Cuvelier, Damien / Chipont, Anna / Allena, Rachele / Guerin, Coralie L / Asnacios, Sophie / Asnacios, Atef / Pierobon, Paolo / Fattaccioli, Jacques

Cell reports methods

2022 Volume 2, Issue 11, Page(s) 100335

Abstract: The immune synapse is the tight contact zone between a lymphocyte and a cell presenting its cognate antigen. This structure serves as a signaling platform and entails a polarization of intracellular components necessary to the immunological function of ... ...

Abstract	The immune synapse is the tight contact zone between a lymphocyte and a cell presenting its cognate antigen. This structure serves as a signaling platform and entails a polarization of intracellular components necessary to the immunological function of the cell. While the surface properties of the presenting cell are known to control the formation of the synapse, their impact on polarization has not yet been studied. Using functional lipid droplets as tunable artificial presenting cells combined with a microfluidic pairing device, we simultaneously observe synchronized synapses and dynamically quantify polarization patterns of individual B cells. By assessing how ligand concentration, surface fluidity, and substrate rigidity impact lysosome polarization, we show that its onset and kinetics depend on the local antigen concentration at the synapse and on substrate rigidity. Our experimental system enables a fine phenotyping of monoclonal cell populations based on their synaptic readout.
MeSH term(s)	Microfluidics ; Lipid Droplets/metabolism ; Immunological Synapses ; Signal Transduction ; B-Lymphocytes ; Antigens/metabolism
Chemical Substances	Antigens
Language	English
Publishing date	2022-11-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2667-2375
ISSN (online)	2667-2375
DOI	10.1016/j.crmeth.2022.100335
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