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  1. Article: pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops.

    Guidelli, Giovanna V / Mattiello, Lucia / Gallinari, Rafael H / Lucca, Paulo Cezar de / Menossi, Marcelo

    Genetics and molecular biology

    2018  Volume 41, Issue 2, Page(s) 450–454

    Abstract: The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and ... ...

    Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene from pGVG showed high levels of GUS activity. qRT-PCR evaluations demonstrated success for both overexpression and hairpin-based silencing cassettes. Therefore, pGVG is suitable for plant transformation and subsequent applications for high-throughput production of stable transgenic sugarcane. The use of an expression cassette based on the ZmUbi1 promoter opens the possibility of using pGVG in other monocot species.
    Language English
    Publishing date 2018-06-11
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 1445712-x
    ISSN 1678-4685 ; 1415-4757
    ISSN (online) 1678-4685
    ISSN 1415-4757
    DOI 10.1590/1678-4685-GMB-2017-0262
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3079: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus.

    Evangelista, Cláudia Carolina Silva / Guidelli, Giovanna Vieira / Borges, Gustavo / Araujo, Thais Fenz / Souza, Tiago Alves Jorge de / Neves, Ubiraci Pereira da Costa / Tunnacliffe, Alan / Pereira, Tiago Campos

    Genetics and molecular biology

    2017  Volume 40, Issue 4, Page(s) 790–802

    Abstract: The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by ... ...

    Abstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.
    Language English
    Publishing date 2017-11-06
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 1445712-x
    ISSN 1678-4685 ; 1415-4757
    ISSN (online) 1678-4685
    ISSN 1415-4757
    DOI 10.1590/1678-4685-GMB-2017-0030
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3079: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: A novel insight on SARS-CoV-2 S-derived fragments in the control of the host immunity.

    Bastos, Thais Sibioni Berti / de Paula, André Guilherme Portela / Dos Santos Luz, Rebeca Bosso / Garnique, Anali M B / Belo, Marco A A / Eto, Silas Fernandes / Fernandes, Dayanne Carla / Ferraris, Fausto Klabund / de Pontes, Leticia Gomes / França, Tábata Takahashi / Barcellos, Leonardo José Gil / Veras, Flavio P / Bermejo, Pamela / Guidelli, Giovanna / Maneira, Carla / da Silveira Bezerra de Mello, Fellipe / Teixeira, Gleidson / Pereira, Gonçalo Amarante Guimarães / Fernandes, Bianca H Ventura /
    Sanches, Paulo R S / Braz, Helyson Lucas Bezerra / Jorge, Roberta Jeane Bezerra / Malafaia, Guilherme / Cilli, Eduardo M / Olivier, Danilo da Silva / do Amaral, Marcos Serrou / Medeiros, Renata J / Condino-Neto, Antonio / Carvalho, Luciani R / Machado-Santelli, Glaucia M / Charlie-Silva, Ives / Galindo-Villegas, Jorge / Braga, Tárcio Teodoro

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 8060

    Abstract: Despite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened health care system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of the disease ...

    Abstract Despite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened health care system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of the disease is crucial for the development of new technologies and therapies for the best clinical management of patients. Since the manipulation of the whole virus requires a structure with an adequate level of biosafety, the development of alternative technologies, such as the synthesis of peptides from viral proteins, is a possible solution to circumvent this problem. In addition, the use and validation of animal models is of extreme importance to screen new drugs and to compress the organism's response to the disease. Peptides derived from recombinant S protein from SARS-CoV-2 were synthesized and validated by in silico, in vitro and in vivo methodologies. Macrophages and neutrophils were challenged with the peptides and the production of inflammatory mediators and activation profile were evaluated. These peptides were also inoculated into the swim bladder of transgenic zebrafish larvae at 6 days post fertilization (dpf) to mimic the inflammatory process triggered by the virus, which was evaluated by confocal microscopy. In addition, toxicity and oxidative stress assays were also developed. In silico and molecular dynamics assays revealed that the peptides bind to the ACE2 receptor stably and interact with receptors and adhesion molecules, such as MHC and TCR, from humans and zebrafish. Macrophages stimulated with one of the peptides showed increased production of NO, TNF-α and CXCL2. Inoculation of the peptides in zebrafish larvae triggered an inflammatory process marked by macrophage recruitment and increased mortality, as well as histopathological changes, similarly to what is observed in individuals with COVID-19. The use of peptides is a valuable alternative for the study of host immune response in the context of COVID-19. The use of zebrafish as an animal model also proved to be appropriate and effective in evaluating the inflammatory process, comparable to humans.
    MeSH term(s) Animals ; Humans ; SARS-CoV-2 ; COVID-19 ; Zebrafish ; Macrophages ; Peptides
    Chemical Substances Peptides
    Language English
    Publishing date 2023-05-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-29588-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Author Correction: A novel insight on SARS-CoV-2 S-derived fragments in the control of the host immunity.

    Bastos, Thais Sibioni Berti / de Paula, André Guilherme Portela / Dos Santos Luz, Rebeca Bosso / Garnique, Anali M B / Belo, Marco A A / Eto, Silas Fernandes / Fernandes, Dayanne Carla / Ferraris, Fausto Klabund / de Pontes, Leticia Gomes / França, Tábata Takahashi / Barcellos, Leonardo José Gil / Veras, Flavio P / Bermejo, Pamela / Guidelli, Giovanna / Maneira, Carla / da Silveira Bezerra de Mello, Fellipe / Teixeira, Gleidson / Pereira, Gonçalo Amarante Guimarães / Fernandes, Bianca H Ventura /
    Sanches, Paulo R S / Braz, Helyson Lucas Bezerra / Jorge, Roberta Jeane Bezerra / Malafaia, Guilherme / Cilli, Eduardo M / Olivier, Danilo da Silva / do Amaral, Marcos Serrou / Medeiros, Renata J / Condino-Neto, Antonio / Carvalho, Luciani R / Machado-Santelli, Glaucia M / Charlie-Silva, Ives / Galindo-Villegas, Jorge / Braga, Tárcio Teodoro

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 12012

    Language English
    Publishing date 2023-07-25
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-39134-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions.

    Ferreira, Thais H S / Tsunada, Max S / Bassi, Denis / Araújo, Pedro / Mattiello, Lucia / Guidelli, Giovanna V / Righetto, Germanna L / Gonçalves, Vanessa R / Lakshmanan, Prakash / Menossi, Marcelo

    Frontiers in plant science

    2017  Volume 8, Page(s) 1077

    Abstract: Sugarcane is a unique crop with the ability to accumulate high levels of sugar and is a commercially viable source of biomass for bioelectricity and second-generation bioethanol. Water deficit is the single largest abiotic stress affecting sugarcane ... ...

    Abstract Sugarcane is a unique crop with the ability to accumulate high levels of sugar and is a commercially viable source of biomass for bioelectricity and second-generation bioethanol. Water deficit is the single largest abiotic stress affecting sugarcane productivity and the development of water use efficient and drought tolerant cultivars is an imperative for all major sugarcane producing countries. This review summarizes the physiological and molecular studies on water deficit stress in sugarcane, with the aim to help formulate more effective research strategies for advancing our knowledge on genes and mechanisms underpinning plant response to water stress. We also overview transgenic studies in sugarcane, with an emphasis on the potential strategies to develop superior sugarcane varieties that improve crop productivity in drought-prone environments.
    Language English
    Publishing date 2017-06-23
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2017.01077
    Database MEDical Literature Analysis and Retrieval System OnLINE

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