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Article ; Online: Cysteine-Rich Hydrophobin Gene Family

Xiao Li / Fen Wang / Yanyan Xu / Guijun Liu / Caihong Dong

International Journal of Molecular Sciences, Vol 22, Iss 2, p

Genome Wide Analysis, Phylogeny and Transcript Profiling in Cordyceps militaris

2021 Volume 643

Abstract: Hydrophobins are a family of small secreted proteins found exclusively in fungi, and they play various roles in the life cycle. In the present study, genome wide analysis and transcript profiling of the hydrophobin family in Cordyceps militaris , a well- ... ...

Abstract	Hydrophobins are a family of small secreted proteins found exclusively in fungi, and they play various roles in the life cycle. In the present study, genome wide analysis and transcript profiling of the hydrophobin family in Cordyceps militaris , a well-known edible and medicinal mushroom, were studied. The distribution of hydrophobins in ascomycetes with different lifestyles showed that pathogenic fungi had significantly more hydrophobins than saprotrophic fungi, and class II members accounted for the majority. Phylogenetic analysis of hydrophobin proteins from the species of Cordyceps s.l. indicated that there was more variability among the class II members than class I. Only a few hydrophobin-encoding genes evolved by duplication in Cordyceps s.l., which was inconsistent with the important role of gene duplication in basidiomycetes. Different transcript patterns of four hydrophobin-encoding genes during the life cycle indicated the possible different functions for each. The transcripts of Cmhyd2 , 3 and 4 can respond to light and were related with the photoreceptors. CmQHYD, with four hydrophobin II domains, was first found in C. militaris , and multi-domain hydrophobins were only distributed in the species of Cordycipitaceae and Clavicipitaceae . These results could be helpful for further function research of hydrophobins and could provide valuable information for the evolution of hydrophobins.
Keywords	Cordyceps militaris ; hydrophobin ; lifestyles ; fruiting body development ; light response ; multi-domain hydrophobin ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Jia-Ji Electro-Acupuncture Improves Locomotor Function With Spinal Cord Injury by Regulation of Autophagy Flux and Inhibition of Necroptosis.

Hongna, Yin / Hongzhao, Tian / Quan, Li / Delin, Feng / Guijun, Liu / Xiaolin, Lv / Fulin, Guan / Zhongren, Sun

Frontiers in neuroscience

2021 Volume 14, Page(s) 616864

Abstract: Jia-Ji electro-acupuncture (EA) has been widely applied in clinic to exhibit curative effects on spinal cord injury (SCI). However, its underlying mechanisms leading to improvement of motor function after SCI remain unclear. Allen's method was made by ... ...

Abstract	Jia-Ji electro-acupuncture (EA) has been widely applied in clinic to exhibit curative effects on spinal cord injury (SCI). However, its underlying mechanisms leading to improvement of motor function after SCI remain unclear. Allen's method was made by NYU Impactor M-III equipment to create the SCI rats model. Rats were randomly divided into four groups: Sham (only laminectomy), Model (SCI group), EA (SCI + Jia-Ji EA treatment), EA + CQ (SCI + Jia-Ji EA treatment + inhibitor chloroquine). Basso-Beattie-Bresnahan assessment showed improvement of hind limb motor function after Jia-Ji electro-acupuncture treatment. Histological change of injured spinal cord tissue was alleviated after treatment, observed by hematoxylin-eosin and Nissl staining. The mRNA and protein expression levels of RIPK1, RIPK3 and MLKL were decreased in EA group. Besides, the increased expression of LC3 and reduced expression of P62 after treatment compared with Model group, confirmed that Jia-Ji electro-acupuncture could enhance the autophagy flux. Electron microscopy imaging showed increasing the number of lysosomes, autophagosomes, and autolysosomes after Jia-Ji electro-acupuncture treatment. Furthermore, inhibition of lysosome function with CQ led to partly eliminate the effect of EA on reducing necroptosis. These data make the case that Jia-Ji electro-acupuncture treatment may improve locomotor function by promoting autophagy flux and inhibiting necroptosis.
Language	English
Publishing date	2021-01-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2411902-7
ISSN	1662-453X ; 1662-4548
ISSN (online)	1662-453X
ISSN	1662-4548
DOI	10.3389/fnins.2020.616864
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

Xuxiao He / Min Li / Hongming Yu / Guijun Liu / Ningning Wang / Chunzhao Yin / Qiaochu Tu / Goutham Narla / Yongzhen Tao / Shuqun Cheng / Huiyong Yin

PLoS Biology, Vol 18, Iss 12, p e

2020 Volume 3000803

Abstract: Loss of hepatic fructose-1, 6-bisphosphate aldolase B (Aldob) leads to a paradoxical up-regulation of glucose metabolism to favor hepatocellular carcinogenesis (HCC), but the upstream signaling events remain poorly defined. Akt is highly activated in HCC, ...

Abstract	Loss of hepatic fructose-1, 6-bisphosphate aldolase B (Aldob) leads to a paradoxical up-regulation of glucose metabolism to favor hepatocellular carcinogenesis (HCC), but the upstream signaling events remain poorly defined. Akt is highly activated in HCC, and targeting Akt is being explored as a potential therapy for HCC. Herein, we demonstrate that Aldob suppresses Akt activity and tumor growth through a protein complex containing Aldob, Akt, and protein phosphatase 2A (PP2A), leading to inhibition of cell viability, cell cycle progression, glucose uptake, and metabolism. Interestingly, Aldob directly interacts with phosphorylated Akt (p-Akt) and promotes the recruitment of PP2A to dephosphorylate p-Akt, and this scaffolding effect of Aldob is independent of its enzymatic activity. Loss of Aldob or disruption of Aldob/Akt interaction in Aldob R304A mutant restores Akt activity and tumor-promoting effects. Consistently, Aldob and p-Akt expression are inversely correlated in human HCC tissues, and Aldob down-regulation coupled with p-Akt up-regulation predicts a poor prognosis for HCC. We have further discovered that Akt inhibition or a specific small-molecule activator of PP2A (SMAP) efficiently attenuates HCC tumorigenesis in xenograft mouse models. Our work reveals a novel nonenzymatic role of Aldob in negative regulation of Akt activation, suggesting that directly inhibiting Akt activity or through reactivating PP2A may be a potential therapeutic approach for HCC treatment.
Keywords	Biology (General) ; QH301-705.5
Subject code	572
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Yeast β-D-glucan exerts antitumour activity in liver cancer through impairing autophagy and lysosomal function, promoting reactive oxygen species production and apoptosis

Ningning Wang / Hongzhi Liu / Guijun Liu / Min Li / Xuxiao He / Chunzhao Yin / Qiaochu Tu / Xia Shen / Wenqiang Bai / Qiang Wang / Yongzhen Tao / Huiyong Yin

Redox Biology, Vol 32, Iss , Pp - (2020)

2020

Abstract: Autophagy is an evolutionarily conserved catabolic process that recycles proteins and organelles in a lysosome-dependent manner and is induced as an alternative source of energy and metabolites in response to diverse stresses. Inhibition of autophagy has ...

Abstract	Autophagy is an evolutionarily conserved catabolic process that recycles proteins and organelles in a lysosome-dependent manner and is induced as an alternative source of energy and metabolites in response to diverse stresses. Inhibition of autophagy has emerged as an appealing therapeutic strategy in cancer. However, it remains to be explored whether autophagy inhibition is a viable approach for the treatment of hepatocellular carcinoma (HCC). Here, we identify that water-soluble yeast β-D-glucan (WSG) is a novel autophagy inhibitor and exerts significant antitumour efficacy on the inhibition of HCC cells proliferation and metabolism as well as the tumour growth in vivo. We further reveal that WSG inhibits autophagic degradation by increasing lysosomal pH and inhibiting lysosome cathepsins (cathepsin B and cathepsin D) activities, which results in the accumulation of damaged mitochondria and reactive oxygen species (ROS) production. Furthermore, WSG sensitizes HCC cells to apoptosis via the activation of caspase 8 and the transfer of truncated BID (tBID) into mitochondria under nutrient deprivation condition. Of note, administration of WSG as a single agent achieves a significant antitumour effect in xenograft mouse model and DEN/CCl4 (diethylnitrosamine/carbon tetrachloride)-induced primary HCC model without apparent toxicity. Our studies reveal, for the first time, that WSG is a novel autophagy inhibitor with significant antitumour efficacy as a single agent, which has great potential in clinical application for liver cancer therapy.
Keywords	β-D-glucan ; Autophagy inhibition ; Lysosomal function ; Hepatocellular carcinoma ; Antitumour ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2020-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Polyphenolic Proanthocyanidin-B2 suppresses proliferation of liver cancer cells and hepatocellular carcinogenesis through directly binding and inhibiting AKT activity

Guijun Liu / Aimin Shi / Ningning Wang / Min Li / Xuxiao He / Chunzhao Yin / Qiaochu Tu / Xia Shen / Yongzhen Tao / Qiang Wang / Huiyong Yin

Redox Biology, Vol 37, Iss , Pp 101701- (2020)

2020

Abstract: The well-documented anticarcinogenic properties of natural polyphenolic proanthocyanidins (OPC) have been primarily attributed to their antioxidant and anti-inflammatory potency. Emerging evidence suggests that OPC may target canonical oncogenic pathways, ...

Abstract	The well-documented anticarcinogenic properties of natural polyphenolic proanthocyanidins (OPC) have been primarily attributed to their antioxidant and anti-inflammatory potency. Emerging evidence suggests that OPC may target canonical oncogenic pathways, including PI3K/AKT; however, the underlying mechanism and therapeutic potential remain elusive. Here we identify that proanthocyanidin B2 (OPC–B2) directly binds and inhibits AKT activity and downstream signalling, thereby suppressing tumour cell proliferation and metabolism in vitro and in a xenograft and diethyl-nitrosamine (DEN)-induced hepatocellular carcinoma (HCC) mouse models. We further find that OPC-B2 binds to the catalytic and regulatory PH domains to lock the protein in a closed conformation, similar to the well-studied AKT allosteric inhibitor MK-2206. Molecular docking and dynamic simulation suggest that Lys297 and Arg86 are critical sites of OPC-B2 binding; mutation of Lys297 or Arg86 to alanine completely abolishes the antitumor effects of OPC-B2 but not MK-2206. Together, our study reveals that OPC-B2 is a novel allosteric AKT inhibitor with potent anti-tumour efficacy beyond its antioxidant and anti-inflammatory properties.
Keywords	Proanthocyanidin B2 (OPC–B2) ; AKT ; Hepatocellular carcinogenesis (HCC) ; Metabolic reprogramming ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Isolation and characterization of antagonistic endophytes from Dendrobium candidum Wall ex Lindl., and the biofertilizing potential of a novel Pseudomonas saponiphila strain

Wu, Liqin / Guijun Liu / Haike Gu / Hongzhong Shang / Qing Wang / Suling Yang

Applied soil ecology. 2016 Sept., v. 105

2016

Abstract: Many endophytic bacterial strains are now successfully used for plant-growth promotion (PGP) and as biocontrol agents (BCA) against plant diseases. This is a promising strategy for the improvement of crop productivity, thereby reducing the use of ... ...

Abstract	Many endophytic bacterial strains are now successfully used for plant-growth promotion (PGP) and as biocontrol agents (BCA) against plant diseases. This is a promising strategy for the improvement of crop productivity, thereby reducing the use of chemical pesticides and fertilizers. The objectives of this study were to isolate endophytic bacteria from the medicinal plant Dendrobium candidum Wall ex Lindl in China, and evaluate the biological control and PGP characteristics of these bacteria. Antagonistic tests were performed against fungal pathogens. Twenty-two of the 83 bacterial isolates showed antagonistic activities. These antagonistic isolates were screened in vitro for PGP traits, such as the production of indoleacetic acid (IAA), siderophores, proteases, and phosphate solubilization. Based on the tested PGP traits, strain YW was selected for further study. Strain YW was identified as Pseudomonas saponiphila based on its 16S rRNA and gyrB gene sequences. We detected the antifungal metabolite-encoding genes phlD, and hcnBC genes encoding 2,4-diacetylphloroglucinol (2,4-DAPG) and hydrogen cyanide (HCN) from strain YW. We measured the effect of strain YW on the growth of pepper seedlings under greenhouse conditions. Our results showed that its antifungal potential may be attributed to 2,4-DAPG and HCN antibiotics, as well as protease and siderophore production, and growth promotion may be related to the ability of isolate YW to synthesize IAA and solubilize phosphate. The PGP and antifungal activities of strain YW suggest that it may be exploited as a potential bioinoculant agent. This is the first report of a P. saponiphila strain with PGP attributes.
Keywords	antibiotics ; antifungal properties ; bacteria ; biological control ; biological control agents ; Dendrobium ; endophytes ; fertilizers ; fungi ; genes ; greenhouse production ; growth promotion ; hydrogen cyanide ; indole acetic acid ; medicinal plants ; nucleotide sequences ; pathogens ; pepper ; phosphates ; plant diseases and disorders ; plant growth ; proteinases ; Pseudomonas ; ribosomal RNA ; seedlings ; siderophores ; solubilization ; China
Language	English
Dates of publication	2016-09
Size	p. 101-108.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1196758-4
ISSN	0929-1393
ISSN	0929-1393
DOI	10.1016/j.apsoil.2016.04.008
Database	NAL-Catalogue (AGRICOLA)

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Article: Utilization of xylose as a carbon source for mixotrophic growth of Scenedesmus obliquus

Yang, Suling / Guijun Liu / Youting Meng / Ping Wang / Sijing Zhou / Hongzhong Shang

Bioresource technology. 2014 Nov., v. 172

2014

Abstract: Mixotrophic cultivation is one potential mode for microalgae production, and an economically acceptable and environmentally sustainable organic carbon source is essential. The potential use of xylose for culturing Scenedesmus obliquus in a mixotrophic ... ...

Abstract	Mixotrophic cultivation is one potential mode for microalgae production, and an economically acceptable and environmentally sustainable organic carbon source is essential. The potential use of xylose for culturing Scenedesmus obliquus in a mixotrophic mode and physiological features of xylose-grown S. obliquus were studied. S. obliquus had a certain xylose tolerance, and was capable of utilizing xylose for growth. At a xylose concentration of 4gLâ1, the maximal cell density was 2.2gLâ1, being 2.9-fold of that under photoautotrophic condition and arriving to the level of mixotrophic growth using 4gLâ1 glucose. No changes in cellular morphology of the cells grown with or without xylose were detected. Fluorescence emission from photosystem II (PS II) relative to photosystem I (PS I) was decreased in mixotrophic cells, implying that the PSII activity was decreased. The biomass lipid content was enhanced and carbohydrate concentration was decreased, in relation to photoautotrophic controls.
Keywords	Scenedesmus obliquus ; algae culture ; biomass ; carbon ; fluorescence ; glucose ; lipid content ; photosystem I ; photosystem II ; xylose
Language	English
Dates of publication	2014-11
Size	p. 180-185.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2014.08.122
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Opposite role of tumor necrosis factor receptors in dextran sulfate sodium-induced colitis in mice.

Ke Wang / Gencheng Han / Yan Dou / Yi Wang / Guijun Liu / Renxi Wang / He Xiao / Xinying Li / Chunmei Hou / Beifen Shen / Renfeng Guo / Yan Li / Yanchun Shi / Guojiang Chen

PLoS ONE, Vol 7, Iss 12, p e

2012 Volume 52924

Abstract: Tumor necrosis factor-α (TNF-α) is a key factor for the pathogenesis of inflammatory bowel diseases (IBD), whose function is known to be mediated by TNF receptor 1 (TNFR1) or 2. However, the precise role of the two receptors in IBD remains poorly ... ...

Abstract	Tumor necrosis factor-α (TNF-α) is a key factor for the pathogenesis of inflammatory bowel diseases (IBD), whose function is known to be mediated by TNF receptor 1 (TNFR1) or 2. However, the precise role of the two receptors in IBD remains poorly understood. Herein, acute colitis was induced by dextran sulfate sodium (DSS) instillation in TNFR1 or 2-/- mice. TNFR1 ablation led to exacerbation of signs of colitis, including more weight loss, increased mortality, colon shortening and oedema, severe intestinal damage, and higher levels of myeloperoxidase compared to wild-type counterparts. While, TNFR2 deficiency had opposite effects. This discrepancy was reflected by alteration of proinflammatory cytokine and chemokine production in the colons. Importantly, TNFR1 ablation rendered enhanced apoptosis of colonic epithelial cells and TNFR2 deficiency conferred pro-apoptotic effects of lamina propria (LP)-immune cells, as shown by the decreased ratio of Bcl-2/Bax and enhanced nuclear factor (NF)-κB activity.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2012-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Isolation and characterization of antagonistic endophytes from Dendrobium candidum Wall ex Lindl., and the biofertilizing potential of a novel Pseudomonas saponiphila strain

Wu, Liqin / Hongzhong Shang / Qing Wang / Haike Gu / Guijun Liu / Suling Yang

Applied soil ecology

Volume v. 105

Abstract	Many endophytic bacterial strains are now successfully used for plant-growth promotion (PGP) and as biocontrol agents (BCA) against plant diseases. This is a promising strategy for the improvement of crop productivity, thereby reducing the use of chemical pesticides and fertilizers. The objectives of this study were to isolate endophytic bacteria from the medicinal plant Dendrobium candidum Wall ex Lindl in China, and evaluate the biological control and PGP characteristics of these bacteria. Antagonistic tests were performed against fungal pathogens. Twenty-two of the 83 bacterial isolates showed antagonistic activities. These antagonistic isolates were screened in vitro for PGP traits, such as the production of indoleacetic acid (IAA), siderophores, proteases, and phosphate solubilization. Based on the tested PGP traits, strain YW was selected for further study. Strain YW was identified as Pseudomonas saponiphila based on its 16S rRNA and gyrB gene sequences. We detected the antifungal metabolite-encoding genes phlD, and hcnBC genes encoding 2,4-diacetylphloroglucinol (2,4-DAPG) and hydrogen cyanide (HCN) from strain YW. We measured the effect of strain YW on the growth of pepper seedlings under greenhouse conditions. Our results showed that its antifungal potential may be attributed to 2,4-DAPG and HCN antibiotics, as well as protease and siderophore production, and growth promotion may be related to the ability of isolate YW to synthesize IAA and solubilize phosphate. The PGP and antifungal activities of strain YW suggest that it may be exploited as a potential bioinoculant agent. This is the first report of a P. saponiphila strain with PGP attributes.
Language	English
Document type	Article
ISSN	0929-1393
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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