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  1. Article ; Online: Culturing clinical Methanobrevibacter smithii using GG medium in a minimal anaerobe atmosphere

    Pilliol, Virginie / Guindo, Cheick Oumar / Terrer, Elodie / Aboudharam, Gérard / Drancourt, Michel / Grine, Ghiles

    Journal of Microbiological Methods. 2023 Apr., v. 207 p.106704-

    2023  

    Abstract: Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has ... ...

    Abstract Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has routinely relied upon hydrogen‑carbon dioxide-enriched, oxygen-deprived atmospheres. In this study, we developed a medium referred to as “GG”, which allowed for M. smithii growth and isolation by culture in an oxygen-deprived atmosphere, with no supply of either hydrogen or carbon dioxide, making it easier to detect M. smithii by culture in clinical microbiology laboratories.
    Keywords Methanobrevibacter smithii ; carbon dioxide ; digestive system ; hydrogen ; intestinal microorganisms ; methane ; methanogens ; GG medium ; Atmosphere ; Clinical microbiology
    Language English
    Dates of publication 2023-04
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2023.106704
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    Zs.A 1849: Show issues Location:
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  2. Article ; Online: Culturing clinical Methanobrevibacter smithii using GG medium in a minimal anaerobe atmosphere.

    Pilliol, Virginie / Guindo, Cheick Oumar / Terrer, Elodie / Aboudharam, Gérard / Drancourt, Michel / Grine, Ghiles

    Journal of microbiological methods

    2023  Volume 207, Page(s) 106704

    Abstract: Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has ... ...

    Abstract Methanobrevibacter smithii (M. smithii), the most prevalent and abundant gut methanogen, detoxifies hydrogen into methane and is, therefore, of paramount importance for the equilibrium of the gut microbiota. The isolation by culture of M. smithii has routinely relied upon hydrogen‑carbon dioxide-enriched, oxygen-deprived atmospheres. In this study, we developed a medium referred to as "GG", which allowed for M. smithii growth and isolation by culture in an oxygen-deprived atmosphere, with no supply of either hydrogen or carbon dioxide, making it easier to detect M. smithii by culture in clinical microbiology laboratories.
    MeSH term(s) Methanobrevibacter ; Carbon Dioxide ; Bacteria, Anaerobic ; Gastrointestinal Microbiome ; Hydrogen
    Chemical Substances Carbon Dioxide (142M471B3J) ; Hydrogen (7YNJ3PO35Z)
    Language English
    Publishing date 2023-03-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2023.106704
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Rapid identification of clinically interesting methanogens using an improved MALDI-TOF-MS assay.

    Guindo, Cheick Oumar / Amir, Lynda / Couderc, Carine / Drancourt, Michel / Grine, Ghiles

    Access microbiology

    2022  Volume 4, Issue 7, Page(s) acmi000372

    Abstract: Methanogens, the archaea uniquely detoxifying fermentative hydrogen into methane in the digestive tract, are increasingly being detected in pathology situations, rendering their rapid identification mandatory. We improved the experimental protocol to ... ...

    Abstract Methanogens, the archaea uniquely detoxifying fermentative hydrogen into methane in the digestive tract, are increasingly being detected in pathology situations, rendering their rapid identification mandatory. We improved the experimental protocol to identify broth-cultured methanogens by matrix-assisted laser desorption time-of-flight MS (MALDI-TOF-MS). A database incorporating 34 reference spectra derived from 16 methanogen reference strains representative of eight species supported further identification of 21
    Language English
    Publishing date 2022-07-12
    Publishing country England
    Document type Journal Article
    ISSN 2516-8290
    ISSN (online) 2516-8290
    DOI 10.1099/acmi.0.000372
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  4. Article ; Online: A

    Guindo, Cheick Oumar / Morsli, Madjid / Bellali, Sara / Drancourt, Michel / Grine, Ghiles

    Current research in microbial sciences

    2022  Volume 3, Page(s) 100112

    Abstract: Tetragenococcus ... ...

    Abstract Tetragenococcus halophilus
    Language English
    Publishing date 2022-02-16
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2666-5174
    ISSN (online) 2666-5174
    DOI 10.1016/j.crmicr.2022.100112
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Candidatus

    Pilliol, Virginie / Morsli, Madjid / Terlier, Laureline / Hassani, Yasmine / Malat, Ihab / Guindo, Cheick Oumar / Davoust, Bernard / Lamglait, Benjamin / Drancourt, Michel / Aboudharam, Gérard / Grine, Ghiles / Terrer, Elodie

    Microbiology spectrum

    2024  Volume 12, Issue 2, Page(s) e0514122

    Abstract: Methanosphaera ... ...

    Abstract Methanosphaera stadtmanae
    MeSH term(s) Humans ; Animals ; Swine ; Macropodidae/genetics ; Methanobacteriaceae/genetics ; Methane ; Feces ; Hydrogen ; Ethanol ; Phylogeny ; RNA, Ribosomal, 16S/genetics
    Chemical Substances Methane (OP0UW79H66) ; Hydrogen (7YNJ3PO35Z) ; Ethanol (3K9958V90M) ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-01-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.05141-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Diversity of Methanogens in Animals' Gut.

    Guindo, Cheick Oumar / Davoust, Bernard / Drancourt, Michel / Grine, Ghiles

    Microorganisms

    2020  Volume 9, Issue 1

    Abstract: Methanogens are members of anaerobe microbiota of the digestive tract of mammals, including humans. However, the sources, modes of acquisition, and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the ... ...

    Abstract Methanogens are members of anaerobe microbiota of the digestive tract of mammals, including humans. However, the sources, modes of acquisition, and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the spectrum of animals that could be sources of methanogens for humans by exploring methanogen carriage in animals. We used real-time PCR, PCR-sequencing, and multispacer sequence typing to investigate the presence of methanogens in 407 fecal specimens collected from nine different mammalian species investigated here. While all the negative controls remained negative, we obtained by PCR-sequencing seven different species of methanogens, of which three (
    Language English
    Publishing date 2020-12-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9010013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Diversity of Methanogens in Animals’ Gut

    Guindo, Cheick Oumar / Davoust, Bernard / Drancourt, Michel / Grine, Ghiles

    Microorganisms. 2020 Dec. 23, v. 9, no. 1

    2020  

    Abstract: Methanogens are members of anaerobe microbiota of the digestive tract of mammals, including humans. However, the sources, modes of acquisition, and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the ... ...

    Abstract Methanogens are members of anaerobe microbiota of the digestive tract of mammals, including humans. However, the sources, modes of acquisition, and dynamics of digestive tract methanogens remain poorly investigated. In this study, we aimed to expand the spectrum of animals that could be sources of methanogens for humans by exploring methanogen carriage in animals. We used real-time PCR, PCR-sequencing, and multispacer sequence typing to investigate the presence of methanogens in 407 fecal specimens collected from nine different mammalian species investigated here. While all the negative controls remained negative, we obtained by PCR-sequencing seven different species of methanogens, of which three (Methanobrevibacter smithii, Methanobrevibacter millerae and Methanomassiliicoccus luminyensis) are known to be part of the methanogens present in the human digestive tract. M. smithii was found in 24 cases, including 12/24 (50%) in pigs, 6/24 (25%) in dogs, 4/24 (16.66%) in cats, and 1/24 (4.16%) in both sheep and horses. Genotyping these 24 M. smithii revealed five different genotypes, all known in humans. Our results are fairly representative of the methanogen community present in the digestive tract of certain animals domesticated by humans, and other future studies must be done to try to cultivate methanogens here detected by molecular biology to better understand the dynamics of methanogens in animals and also the likely acquisition of methanogens in humans through direct contact with these animals or through consumption of the meat and/or milk of certain animals, in particular cows.
    Keywords Methanobrevibacter smithii ; cats ; cows ; digestive tract ; direct contact ; dogs ; dynamics ; feces ; genotype ; genotyping ; horses ; humans ; meat ; methanogens ; milk ; molecular biology ; quantitative polymerase chain reaction ; sheep ; swine
    Language English
    Dates of publication 2020-1223
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9010013
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  8. Article ; Online: Azithromycin disrupts apicoplast biogenesis in replicating and dormant liver stages of the relapsing malaria parasites Plasmodium vivax and Plasmodium cynomolgi.

    Amanzougaghene, Nadia / Tajeri, Shahin / Franetich, Jean-François / Ashraf, Kutub / Soulard, Valérie / Bigeard, Pierre / Guindo, Cheick Oumar / Bouillier, Camille / Lemaitre, Julien / Relouzat, Francis / Legrand, Roger / Kocken, Clemens H M / Zeeman, Anne-Marie / Roobsoong, Wanlapa / Sattabongkot, Jetsumon / Yang, Zhaoqing / Snounou, Georges / Mazier, Dominique

    International journal of antimicrobial agents

    2024  Volume 63, Issue 5, Page(s) 107112

    Abstract: The control and elimination of malaria caused by Plasmodium vivax is hampered by the threat of relapsed infection resulting from the activation of dormant hepatic hypnozoites. Currently, only the 8-aminoquinolines, primaquine and tafenoquine, have been ... ...

    Abstract The control and elimination of malaria caused by Plasmodium vivax is hampered by the threat of relapsed infection resulting from the activation of dormant hepatic hypnozoites. Currently, only the 8-aminoquinolines, primaquine and tafenoquine, have been approved for the elimination of hypnozoites, although their use is hampered by potential toxicity. Therefore, an alternative radical curative drug that safely eliminates hypnozoites is a pressing need. This study assessed the potential hypnozoiticidal activity of the antibiotic azithromycin, which is thought to exert antimalarial activity by inhibiting prokaryote-like ribosomal translation within the apicoplast, an indispensable organelle. The results show that azithromycin inhibited apicoplast development during liver-stage schizogony in P. vivax and Plasmodium cynomolgi, leading to impaired parasite maturation. More importantly, this study found that azithromycin is likely to impair the hypnozoite's apicoplast, resulting in the loss of this organelle. Subsequently, using a recently developed long-term hepatocyte culture system, this study found that this loss likely induces a delay in the hypnozoite activation rate, and that those parasites that do proceed to schizogony display liver-stage arrest prior to differentiating into hepatic merozoites, thus potentially preventing relapse. Overall, this work provides evidence for the potential use of azithromycin for the radical cure of relapsing malaria, and identifies apicoplast functions as potential drug targets in quiescent hypnozoites.
    MeSH term(s) Azithromycin/pharmacology ; Plasmodium vivax/drug effects ; Plasmodium cynomolgi/drug effects ; Antimalarials/pharmacology ; Liver/parasitology ; Liver/drug effects ; Apicoplasts/drug effects ; Animals ; Hepatocytes/parasitology ; Hepatocytes/drug effects ; Humans ; Organelle Biogenesis ; Malaria, Vivax/parasitology ; Malaria, Vivax/drug therapy ; Mice ; Malaria/parasitology ; Malaria/drug therapy
    Chemical Substances Azithromycin (83905-01-5) ; Antimalarials
    Language English
    Publishing date 2024-02-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1093977-5
    ISSN 1872-7913 ; 0924-8579
    ISSN (online) 1872-7913
    ISSN 0924-8579
    DOI 10.1016/j.ijantimicag.2024.107112
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 500: Show issues

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  9. Article ; Online: Meconial

    Sereme, Youssouf / Guindo, Cheick Oumar / Filleron, Anne / Corbeau, Pierre / Tran, Tu Anh / Drancourt, Michel / Vitte, Joana / Grine, Ghiles

    Current research in microbial sciences

    2021  Volume 2, Page(s) 100034

    Abstract: To understand the dynamics of methanogens in the human intestinal microbiota, we investigated the presence of methanogens in meconium using a polyphasic approach including microscopy and PCR-sequencing in 33 meconium samples collected from 33 pre-term ... ...

    Abstract To understand the dynamics of methanogens in the human intestinal microbiota, we investigated the presence of methanogens in meconium using a polyphasic approach including microscopy and PCR-sequencing in 33 meconium samples collected from 33 pre-term neonates, in accordance with current ethics regulation. In the presence of negative controls, 90.9% samples were real-time PCR-positive for methanogens and 69.7 % were PCR-sequencing positive, identified as
    Language English
    Publishing date 2021-04-29
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2666-5174
    ISSN (online) 2666-5174
    DOI 10.1016/j.crmicr.2021.100034
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  10. Article: A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases.

    Sereme, Youssouf / Michel, Moïse / Mezouar, Soraya / Guindo, Cheick Oumar / Kaba, Lanceï / Grine, Ghiles / Mura, Thibault / Mège, Jean-Louis / Tran, Tu Anh / Corbeau, Pierre / Filleron, Anne / Vitte, Joana

    Journal of clinical medicine

    2022  Volume 11, Issue 10

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2022-05-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm11102749
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