LIVIVO - Search results -

Search results

Result 1 - 10 of total 21

Search options

Article ; Online: Activated immune cells drive neurodegeneration in an Alzheimer's model.

Guldner, Ian H / Wyss-Coray, Tony

2023 Volume 615, Issue 7953, Page(s) 588–589

MeSH term(s)	Humans ; Alzheimer Disease ; Amyloid beta-Peptides ; Positron-Emission Tomography
Chemical Substances	Amyloid beta-Peptides
Language	English
Publishing date	2023-03-08
Publishing country	England
Document type	News ; Comment
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/d41586-023-00600-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 26: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 9: Show issues
Zs.MO 244: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A nanotherapeutic approach to selectively eliminate metastatic breast cancer cells by targeting cell surface GRP78.

Shin, Jaeho / Kim, Baksun / Lager, Tyson W / Mejia, Franklin / Guldner, Ian / Conner, Clay / Zhang, Siyuan / Panopoulos, Athanasia D / Bilgicer, Basar

Nanoscale

2023 Volume 15, Issue 32, Page(s) 13322–13334

Abstract: Here, rational engineering of doxorubicin prodrug loaded peptide-targeted liposomal nanoparticles to selectively target metastatic breast cancer ... ...

Abstract	Here, rational engineering of doxorubicin prodrug loaded peptide-targeted liposomal nanoparticles to selectively target metastatic breast cancer cells
MeSH term(s)	Animals ; Mice ; Endoplasmic Reticulum Chaperone BiP ; Membrane Proteins ; Cell Line, Tumor ; Prodrugs ; Glucose ; Peptides ; Doxorubicin/pharmacology ; Neoplasms
Chemical Substances	Endoplasmic Reticulum Chaperone BiP ; Membrane Proteins ; Prodrugs ; Glucose (IY9XDZ35W2) ; Peptides ; Doxorubicin (80168379AG)
Language	English
Publishing date	2023-08-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/d3nr00800b
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes.

Guldner, Ian H / Golomb, Samantha M / Wang, Qingfei / Wang, Emilia / Zhang, Siyuan

STAR protocols

2021 Volume 2, Issue 2, Page(s) 100537

Abstract: High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries. High-quality single-cell leukocyte preparations are a prerequisite for optimal single ... ...

Abstract	High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries. High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling. Here, we describe a protocol for epitope and RNA-preserving dissociation of adult mouse brains and subsequent leukocyte purification and staining, which is adaptable to homeostatic and pathogenic brains. Leukocyte preparation following this protocol permits exquisite single-cell surface protein and RNA profiling in applications including CyTOF and CITE-seq. For complete details on the use and execution of this protocol, please refer to Guldner et al. (2020) and Golomb et al. (2020).
MeSH term(s)	Animals ; Brain/pathology ; Cell Separation/methods ; Cells, Cultured ; Epitopes/genetics ; Female ; Leukocytes/cytology ; Leukocytes/metabolism ; Male ; Mice ; Single-Cell Analysis/methods ; Transcriptome/genetics
Chemical Substances	Epitopes
Language	English
Publishing date	2021-05-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2021.100537
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: A journey to uncharted territory: new technical frontiers in studying tumor–stromal cell interactions

Guldner, Ian H / Zhang, Siyuan

Integrative biology. 2015 Feb. 10, v. 7, no. 2

2015

Abstract: The crosstalk between tumor cells and cells of the tumor stroma dictate malignant progression and represent an intriguing and viable anticancer therapeutic target. The successful development of therapeutics targeting tumor–stroma interactions is tied to ... ...

Abstract	The crosstalk between tumor cells and cells of the tumor stroma dictate malignant progression and represent an intriguing and viable anticancer therapeutic target. The successful development of therapeutics targeting tumor–stroma interactions is tied to the insight provided by basic research on such crosstalk. Tumor–stroma interactions can be transient and dynamic, and they occur within defined spatiotemporal contexts among genetically and compositionally heterogeneous populations of cells, yet methods currently applied to study the said crosstalk do not sufficiently address these features. Emerging imaging and genetic methods, however, can overcome limitations of traditional approaches and provide unprecedented insight into tumor–stroma crosstalk with unparalleled accuracy. The comprehensive data obtained by applying emerging methods will require processing and analysis by multidisciplinary teams, but the efforts will ultimately rejuvenate hope in developing novel therapies against pro-tumorigenic tumor–stroma crosstalk.
Keywords	cancer therapy ; image analysis ; neoplasm cells ; neoplasms
Language	English
Dates of publication	2015-0210
Size	p. 153-161.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2480063-6
ISSN	1757-9708 ; 1757-9694
ISSN (online)	1757-9708
ISSN	1757-9694
DOI	10.1039/c4ib00192c
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Targeting CXCL16 and STAT1 augments immune checkpoint blockade therapy in triple-negative breast cancer.

Palakurthi, Bhavana / Fross, Shaneann R / Guldner, Ian H / Aleksandrovic, Emilija / Liu, Xiyu / Martino, Anna K / Wang, Qingfei / Neff, Ryan A / Golomb, Samantha M / Lewis, Cheryl / Peng, Yan / Howe, Erin N / Zhang, Siyuan

Nature communications

2023 Volume 14, Issue 1, Page(s) 2109

Abstract: Chemotherapy prior to immune checkpoint blockade (ICB) treatment appears to improve ICB efficacy but resistance to ICB remains a clinical challenge and is attributed to highly plastic myeloid cells associating with the tumor immune microenvironment (TIME) ...

Abstract	Chemotherapy prior to immune checkpoint blockade (ICB) treatment appears to improve ICB efficacy but resistance to ICB remains a clinical challenge and is attributed to highly plastic myeloid cells associating with the tumor immune microenvironment (TIME). Here we show by CITE-seq single-cell transcriptomic and trajectory analyses that neoadjuvant low-dose metronomic chemotherapy (MCT) leads to a characteristic co-evolution of divergent myeloid cell subsets in female triple-negative breast cancer (TNBC). Specifically, we identify that the proportion of CXCL16 + myeloid cells increase and a high STAT1 regulon activity distinguishes Programmed Death Ligand 1 (PD-L1) expressing immature myeloid cells. Chemical inhibition of STAT1 signaling in MCT-primed breast cancer sensitizes TNBC to ICB treatment, which underscores the STAT1's role in modulating TIME. In summary, we leverage single-cell analyses to dissect the cellular dynamics in the tumor microenvironment (TME) following neoadjuvant chemotherapy and provide a pre-clinical rationale for modulating STAT1 in combination with anti-PD-1 for TNBC patients.
MeSH term(s)	Humans ; Female ; Triple Negative Breast Neoplasms/drug therapy ; Triple Negative Breast Neoplasms/genetics ; Immune Checkpoint Inhibitors/pharmacology ; Immune Checkpoint Inhibitors/therapeutic use ; Radioimmunotherapy ; Myeloid Cells ; Chemokine CXCL16 ; Tumor Microenvironment ; STAT1 Transcription Factor/genetics
Chemical Substances	Immune Checkpoint Inhibitors ; Chemokine CXCL16 ; STAT1 protein, human ; STAT1 Transcription Factor ; CXCL16 protein, human
Language	English
Publishing date	2023-04-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-37727-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: A journey to uncharted territory: new technical frontiers in studying tumor-stromal cell interactions.

Guldner, Ian H / Zhang, Siyuan

Integrative biology : quantitative biosciences from nano to macro

2014 Volume 7, Issue 2, Page(s) 153–161

Abstract	The crosstalk between tumor cells and cells of the tumor stroma dictate malignant progression and represent an intriguing and viable anticancer therapeutic target. The successful development of therapeutics targeting tumor-stroma interactions is tied to the insight provided by basic research on such crosstalk. Tumor-stroma interactions can be transient and dynamic, and they occur within defined spatiotemporal contexts among genetically and compositionally heterogeneous populations of cells, yet methods currently applied to study the said crosstalk do not sufficiently address these features. Emerging imaging and genetic methods, however, can overcome limitations of traditional approaches and provide unprecedented insight into tumor-stroma crosstalk with unparalleled accuracy. The comprehensive data obtained by applying emerging methods will require processing and analysis by multidisciplinary teams, but the efforts will ultimately rejuvenate hope in developing novel therapies against pro-tumorigenic tumor-stroma crosstalk.
MeSH term(s)	Animals ; Cell Communication/genetics ; Cell Communication/physiology ; Computational Biology ; Diagnostic Imaging ; Humans ; Microscopy ; Neoplasms/genetics ; Neoplasms/pathology ; Neoplasms/physiopathology ; Stromal Cells/pathology ; Stromal Cells/physiology ; Tumor Microenvironment/genetics ; Tumor Microenvironment/physiology
Language	English
Publishing date	2014-12-09
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2480063-6
ISSN	1757-9708 ; 1757-9694
ISSN (online)	1757-9708
ISSN	1757-9694
DOI	10.1039/c4ib00192c
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Aged Breast Extracellular Matrix Drives Mammary Epithelial Cells to an Invasive and Cancer-Like Phenotype.

Bahcecioglu, Gokhan / Yue, Xiaoshan / Howe, Erin / Guldner, Ian / Stack, M Sharon / Nakshatri, Harikrishna / Zhang, Siyuan / Zorlutuna, Pinar

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2021 Volume 8, Issue 22, Page(s) e2100128

Abstract: Age is a major risk factor for cancer. While the importance of age related genetic alterations in cells on cancer progression is well documented, the effect of aging extracellular matrix (ECM) has been overlooked. This study shows that the aging breast ... ...

Abstract	Age is a major risk factor for cancer. While the importance of age related genetic alterations in cells on cancer progression is well documented, the effect of aging extracellular matrix (ECM) has been overlooked. This study shows that the aging breast ECM alone is sufficient to drive normal human mammary epithelial cells (KTB21) to a more invasive and cancer-like phenotype, while promoting motility and invasiveness in MDA-MB-231 cells. Decellularized breast matrix from aged mice leads to loss of E-cadherin membrane localization in KTB21 cells, increased cell motility and invasion, and increased production of inflammatory cytokines and cancer-related proteins. The aged matrix upregulates cancer-related genes in KTB21 cells and enriches a cell subpopulation highly expressing epithelial-mesenchymal transition-related genes. Lysyl oxidase knockdown reverts the aged matrix-induced changes to the young levels; it relocalizes E-cadherin to cell membrane, and reduces cell motility, invasion, and cytokine production. These results show for the first time that the aging ECM harbors key biochemical, physical, and mechanical cues contributing to invasive and cancer-like behavior in healthy and cancer mammary cells. Differential response of cells to young and aged ECMs can lead to identification of new targets for cancer treatment and prevention.
MeSH term(s)	Aging/metabolism ; Animals ; Breast/metabolism ; Breast/pathology ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Disease Models, Animal ; Extracellular Matrix/metabolism ; Female ; Humans ; Mice ; Mice, Inbred C57BL ; Neoplasm Invasiveness ; Phenotype
Language	English
Publishing date	2021-10-07
Publishing country	Germany
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202100128
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Atlas of the aging mouse brain reveals white matter as vulnerable foci.

Hahn, Oliver / Foltz, Aulden G / Atkins, Micaiah / Kedir, Blen / Moran-Losada, Patricia / Guldner, Ian H / Munson, Christy / Kern, Fabian / Pálovics, Róbert / Lu, Nannan / Zhang, Hui / Kaur, Achint / Hull, Jacob / Huguenard, John R / Grönke, Sebastian / Lehallier, Benoit / Partridge, Linda / Keller, Andreas / Wyss-Coray, Tony

Cell

2023 Volume 186, Issue 19, Page(s) 4117–4133.e22

Abstract: Aging is the key risk factor for cognitive decline, yet the molecular changes underlying brain aging remain poorly understood. Here, we conducted spatiotemporal RNA sequencing of the mouse brain, profiling 1,076 samples from 15 regions across 7 ages and ... ...

Abstract	Aging is the key risk factor for cognitive decline, yet the molecular changes underlying brain aging remain poorly understood. Here, we conducted spatiotemporal RNA sequencing of the mouse brain, profiling 1,076 samples from 15 regions across 7 ages and 2 rejuvenation interventions. Our analysis identified a brain-wide gene signature of aging in glial cells, which exhibited spatially defined changes in magnitude. By integrating spatial and single-nucleus transcriptomics, we found that glial aging was particularly accelerated in white matter compared with cortical regions, whereas specialized neuronal populations showed region-specific expression changes. Rejuvenation interventions, including young plasma injection and dietary restriction, exhibited distinct effects on gene expression in specific brain regions. Furthermore, we discovered differential gene expression patterns associated with three human neurodegenerative diseases, highlighting the importance of regional aging as a potential modulator of disease. Our findings identify molecular foci of brain aging, providing a foundation to target age-related cognitive decline.
MeSH term(s)	Animals ; Humans ; Mice ; Aging ; Cognitive Dysfunction/genetics ; Gene Expression Profiling ; Solitary Nucleus ; White Matter/pathology ; Single-Cell Gene Expression Analysis ; Brain/pathology
Language	English
Publishing date	2023-08-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2023.07.027
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1167: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 58: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Single-cell profiling guided combinatorial immunotherapy for fast-evolving CDK4/6 inhibitor-resistant HER2-positive breast cancer.

Wang, Qingfei / Guldner, Ian H / Golomb, Samantha M / Sun, Longhua / Harris, Jack A / Lu, Xin / Zhang, Siyuan

Nature communications

2019 Volume 10, Issue 1, Page(s) 3817

Abstract: Acquired resistance to targeted cancer therapy is a significant clinical challenge. In parallel with clinical trials combining CDK4/6 inhibitors to treat HER2+ breast cancer, we sought to prospectively model tumor evolution in response to this regimen in ...

Abstract	Acquired resistance to targeted cancer therapy is a significant clinical challenge. In parallel with clinical trials combining CDK4/6 inhibitors to treat HER2+ breast cancer, we sought to prospectively model tumor evolution in response to this regimen in vivo and identify a clinically actionable strategy to combat drug resistance. Despite a promising initial response, acquired resistance emerges rapidly to the combination of anti-HER2/neu antibody and CDK4/6 inhibitor Palbociclib. Using high-throughput single-cell profiling over the course of treatments, we reveal a distinct immunosuppressive immature myeloid cell (IMC) population to infiltrate the resistant tumors. Guided by single-cell transcriptome analysis, we demonstrate that combination of IMC-targeting tyrosine kinase inhibitor cabozantinib and immune checkpoint blockade enhances anti-tumor immunity, and overcomes the resistance. Furthermore, sequential combinatorial immunotherapy enables a sustained control of the fast-evolving CDK4/6 inhibitor-resistant tumors. Our study demonstrates a translational framework for treating rapidly evolving tumors through preclinical modeling and single-cell analyses.
MeSH term(s)	Animals ; Antineoplastic Agents, Immunological/pharmacology ; Antineoplastic Agents, Immunological/therapeutic use ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Cell Line, Tumor ; Cyclin-Dependent Kinase 4/antagonists & inhibitors ; Cyclin-Dependent Kinase 6/antagonists & inhibitors ; Drug Resistance, Neoplasm/drug effects ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic/drug effects ; Gene Expression Regulation, Neoplastic/immunology ; Mammary Neoplasms, Experimental/drug therapy ; Mammary Neoplasms, Experimental/immunology ; Mammary Neoplasms, Experimental/pathology ; Mice ; Mice, Transgenic ; Models, Biological ; Myeloid Progenitor Cells/drug effects ; Myeloid Progenitor Cells/immunology ; Protein Kinase Inhibitors/pharmacology ; Protein Kinase Inhibitors/therapeutic use ; Receptor, ErbB-2/antagonists & inhibitors ; Receptor, ErbB-2/genetics ; Receptor, ErbB-2/metabolism ; Single-Cell Analysis ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology ; Treatment Outcome ; Tumor Microenvironment/drug effects ; Tumor Microenvironment/immunology ; Xenograft Model Antitumor Assays
Chemical Substances	Antineoplastic Agents, Immunological ; Protein Kinase Inhibitors ; Erbb2 protein, rat (EC 2.7.10.1) ; Receptor, ErbB-2 (EC 2.7.10.1) ; CDK4 protein, human (EC 2.7.11.22) ; CDK6 protein, human (EC 2.7.11.22) ; Cyclin-Dependent Kinase 4 (EC 2.7.11.22) ; Cyclin-Dependent Kinase 6 (EC 2.7.11.22)
Language	English
Publishing date	2019-08-23
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-019-11729-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: APOE4/4 is linked to damaging lipid droplets in Alzheimer's disease microglia.

Haney, Michael S / Pálovics, Róbert / Munson, Christy Nicole / Long, Chris / Johansson, Patrik K / Yip, Oscar / Dong, Wentao / Rawat, Eshaan / West, Elizabeth / Schlachetzki, Johannes C M / Tsai, Andy / Guldner, Ian Hunter / Lamichhane, Bhawika S / Smith, Amanda / Schaum, Nicholas / Calcuttawala, Kruti / Shin, Andrew / Wang, Yung-Hua / Wang, Chengzhong /

Koutsodendris, Nicole / Serrano, Geidy E / Beach, Thomas G / Reiman, Eric M / Glass, Christopher K / Abu-Remaileh, Monther / Enejder, Annika / Huang, Yadong / Wyss-Coray, Tony

Nature

2024 Volume 628, Issue 8006, Page(s) 154–161

Abstract: Several genetic risk factors for Alzheimer's disease implicate genes involved in lipid metabolism and many of these lipid genes are highly expressed in glial ... ...

Abstract	Several genetic risk factors for Alzheimer's disease implicate genes involved in lipid metabolism and many of these lipid genes are highly expressed in glial cells
MeSH term(s)	Animals ; Female ; Humans ; Male ; Mice ; Alzheimer Disease/genetics ; Alzheimer Disease/metabolism ; Alzheimer Disease/pathology ; Amyloid beta-Peptides/metabolism ; Apolipoprotein E4/genetics ; Apolipoprotein E4/metabolism ; Induced Pluripotent Stem Cells/cytology ; Lipid Droplets/metabolism ; Lipid Droplets/pathology ; Microglia/cytology ; Microglia/metabolism ; Microglia/pathology ; Triglycerides ; tau Proteins ; Culture Media, Conditioned ; Phosphorylation ; Genetic Predisposition to Disease
Chemical Substances	Amyloid beta-Peptides ; Apolipoprotein E4 ; ACSL1 protein, human (EC 6.2.1.3) ; Triglycerides ; tau Proteins ; Culture Media, Conditioned
Language	English
Publishing date	2024-03-13
Publishing country	England
Document type	Journal Article
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/s41586-024-07185-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 26: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 9: Show issues
Zs.MO 244: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito