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  1. Article ; Online: Effect of time post warming to embryo transfer on human blastocyst metabolism and pregnancy outcome.

    Ardestani, Goli / Martins, Marion / Ocali, Olcay / Sanchez, Tim H / Gulliford, Colwyn / Barrett, C Brent / Sakkas, Denny

    Journal of assisted reproduction and genetics

    2024  

    Abstract: Purpose: This study is aiming to test whether variation in post warming culture time impacts blastocyst metabolism or pregnancy outcome.: Methods: In this single center retrospective cohort study, outcomes of 11,520 single frozen embryo transfer (FET) ...

    Abstract Purpose: This study is aiming to test whether variation in post warming culture time impacts blastocyst metabolism or pregnancy outcome.
    Methods: In this single center retrospective cohort study, outcomes of 11,520 single frozen embryo transfer (FET) cycles were analyzed from January 2015 to December 2020. Patient treatments included both natural and programmed cycles. Time categories were determined using the time between blastocyst warming and embryo transfer: 0 (0- <1h), 1 (1-<2h), 2 (2-<3h), 3(3-<4h), 4 (4-<5), 5 (5-<6), 6 (6-<7) and 7 (7-8h). Non-invasive metabolic imaging of discarded human blastocysts for up to 10h was also performed using Fluorescence lifetime imaging microscopy (FLIM) to examine for metabolic perturbations during culture.
    Results: The mean age of patients across all time categories were comparable (35.6 ± 3.9). Live birth rates (38-52%) and miscarriage rate (5-11%) were not statistically different across post-warming culture time. When assessing pregnancy outcomes based on the use of PGT-A, miscarriage and live birth rates were not statistically different across culture hours in both PGT-A and non-PGT cycles. Further metabolic analysis of blastocysts for the duration of 10h of culture post warming, revealed minimal metabolic changes of embryos in culture.
    Conclusion: Overall, our results show that differences in the time of post warming culture have no significant impact on miscarriage or live birth rate for frozen embryo transfers. This information can be beneficial for clinical practices with either minimal staffing or a high number of patient cases.
    Language English
    Publishing date 2024-04-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1112577-9
    ISSN 1573-7330 ; 1058-0468
    ISSN (online) 1573-7330
    ISSN 1058-0468
    DOI 10.1007/s10815-024-03115-8
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  2. Article ; Online: Metabolic imaging of human embryos is predictive of ploidy status but is not associated with clinical pregnancy outcomes: a pilot trial.

    Sakkas, Denny / Gulliford, Colwyn / Ardestani, Goli / Ocali, Olcay / Martins, Marion / Talasila, Nitya / Shah, Jaimin S / Penzias, Alan S / Seidler, Emily A / Sanchez, Tim

    Human reproduction (Oxford, England)

    2024  Volume 39, Issue 3, Page(s) 516–525

    Abstract: Study question: Does fluorescence lifetime imaging microscopy (FLIM)-based metabolic imaging assessment of human blastocysts prior to frozen transfer correlate with pregnancy outcomes?: Summary answer: FLIM failed to distinguish consistent patterns ... ...

    Abstract Study question: Does fluorescence lifetime imaging microscopy (FLIM)-based metabolic imaging assessment of human blastocysts prior to frozen transfer correlate with pregnancy outcomes?
    Summary answer: FLIM failed to distinguish consistent patterns in mitochondrial metabolism between blastocysts leading to pregnancy compared to those that did not.
    What is known already: FLIM measurements provide quantitative information on NAD(P)H and flavin adenine dinucleotide (FAD+) concentrations. The metabolism of embryos has long been linked to their viability, suggesting the potential utility of metabolic measurements to aid in selection.
    Study design, size, duration: This was a pilot trial enrolling 121 IVF couples who consented to have their frozen blastocyst measured using non-invasive metabolic imaging. After being warmed, 105 couples' good-quality blastocysts underwent a 6-min scan in a controlled temperature and gas environment. FLIM-assessed blastocysts were then transferred without any intervention in management.
    Participants/materials, setting, methods: Eight metabolic parameters were obtained from each blastocyst (4 for NAD(P)H and 4 for FAD): short and long fluorescence lifetime, fluorescence intensity, and fraction of the molecule engaged with enzyme. The redox ratio (intensity of NAD(P)H)/(intensity of FAD) was also calculated. FLIM data were combined with known metadata and analyzed to quantify the ability of metabolic imaging to differentiate embryos that resulted in pregnancy from embryos that did not. De-identified discarded aneuploid human embryos (n = 158) were also measured to quantify correlations with ploidy status and other factors. Statistical comparisons were performed using logistic regression and receiver operating characteristic (ROC) curves with 5-fold cross-validation averaged over 100 repeats with random sampling. AUC values were used to quantify the ability to distinguish between classes.
    Main results and the role of chance: No metabolic imaging parameters showed significant differences between good-quality blastocysts resulting in pregnancy versus those that did not. A logistic regression using metabolic data and metadata produced an ROC AUC of 0.58. In contrast, robust AUCs were obtained when classifying other factors such as comparison of Day 5 (n = 64) versus Day 6 (n = 41) blastocysts (AUC = 0.78), inner cell mass versus trophectoderm (n = 105: AUC = 0.88) and aneuploid (n = 158) versus euploid and positive pregnancy embryos (n = 108) (AUC = 0.82).
    Limitations, reasons for caution: The study protocol did not select which embryo to transfer and the cohort of 105 included blastocysts were all high quality. The study was also limited in number of participants and study sites. Increased power and performing the trial in more sites may have provided a stronger conclusion regarding the merits of the use of FLIM clinically.
    Wider implications of the findings: FLIM failed to distinguish consistent patterns in mitochondrial metabolism between good-quality blastocysts leading to pregnancy compared to those that did not. Blastocyst ploidy status was, however, highly distinguishable. In addition, embryo regions and embryo day were consistently revealed by FLIM. While metabolic imaging detects mitochondrial metabolic features in human blastocysts, this pilot trial indicates it does not have the potential to serve as an effective embryo viability detection tool. This may be because mitochondrial metabolism plays an alternative role post-implantation.
    Study funding/competing interest(s): This study was sponsored by Optiva Fertility, Inc. Boston IVF contributed to the clinical site and services. Becker Hickl, GmbH, provided the FLIM system on loan. T.S. was the founder and held stock in Optiva Fertility, Inc., and D.S. and E.S. had options with Optiva Fertility, Inc., during this study.
    Trial registration number: The study was approved by WCG Connexus IRB (Study Number 1298156).
    MeSH term(s) Female ; Pregnancy ; Humans ; Flavin-Adenine Dinucleotide ; NAD ; Pilot Projects ; Ploidies ; Aneuploidy
    Chemical Substances Flavin-Adenine Dinucleotide (146-14-5) ; NAD (0U46U6E8UK)
    Language English
    Publishing date 2024-01-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 632776-x
    ISSN 1460-2350 ; 0268-1161 ; 1477-741X
    ISSN (online) 1460-2350
    ISSN 0268-1161 ; 1477-741X
    DOI 10.1093/humrep/dead268
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  3. Article ; Online: CBETA: First Multipass Superconducting Linear Accelerator with Energy Recovery.

    Bartnik, A / Banerjee, N / Burke, D / Crittenden, J / Deitrick, K / Dobbins, J / Gulliford, C / Hoffstaetter, G H / Li, Y / Lou, W / Quigley, P / Sagan, D / Smolenski, K / Berg, J S / Brooks, S / Hulsart, R / Mahler, G / Meot, F / Michnoff, R /
    Peggs, S / Roser, T / Trbojevic, D / Tsoupas, N / Miyajima, T

    Physical review letters

    2020  Volume 125, Issue 4, Page(s) 44803

    Abstract: Energy recovery has been achieved in a multipass linear accelerator, demonstrating a technology for more compact particle accelerators operating at higher currents and reduced energy consumption. Energy delivered to the beam during the first four passes ... ...

    Abstract Energy recovery has been achieved in a multipass linear accelerator, demonstrating a technology for more compact particle accelerators operating at higher currents and reduced energy consumption. Energy delivered to the beam during the first four passes through the accelerating structure was recovered during four subsequent decelerating passes. High-energy efficiency was achieved by the use of superconducting accelerating cavities and permanent magnets. The fixed-field alternating-gradient optical system used for the return loop successfully transported electron bunches of 42, 78, 114, and 150 MeV in a common vacuum chamber. This new kind of accelerator, an eight-pass energy recovery linac, has the potential to accelerate much higher current than existing linear accelerators while maintaining small beam dimensions and consuming much less energy per electron.
    Language English
    Publishing date 2020-08-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.125.044803
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  4. Article: A rare case of postmenopausal bleeding due to Wegener's granulomatosis.

    Ahson, Ghadah Z / Parkin, David E / Gulliford, Cathy / Ashok, Premila W

    Acta obstetricia et gynecologica Scandinavica

    2002  Volume 81, Issue 11, Page(s) 1086–1087

    MeSH term(s) Aged ; Aged, 80 and over ; Fatal Outcome ; Female ; Granulomatosis with Polyangiitis/complications ; Granulomatosis with Polyangiitis/diagnosis ; Granulomatosis with Polyangiitis/pathology ; Granulomatosis with Polyangiitis/surgery ; Humans ; Postmenopause ; Uterine Cervical Neoplasms/complications ; Uterine Cervical Neoplasms/diagnosis ; Uterine Cervical Neoplasms/pathology ; Uterine Cervical Neoplasms/surgery ; Uterine Hemorrhage/etiology
    Language English
    Publishing date 2002-11
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 80019-3
    ISSN 1600-0412 ; 0001-6349
    ISSN (online) 1600-0412
    ISSN 0001-6349
    DOI 10.1034/j.1600-0412.2002.811117.x
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  5. Article: Saphenofemoral venous channels associated with recurrent varicose veins are not neovascular.

    El Wajeh, Y / Giannoukas, A D / Gulliford, C J / Suvarna, S K / Chan, P

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery

    2004  Volume 28, Issue 6, Page(s) 590–594

    Abstract: Background: Recurrence of varicose veins after apparently adequate surgery is common. Neovascularisation, the formation of new vascular channels between a venous surgery site and new varicosities, is thought to be an important cause of recurrence. The ... ...

    Abstract Background: Recurrence of varicose veins after apparently adequate surgery is common. Neovascularisation, the formation of new vascular channels between a venous surgery site and new varicosities, is thought to be an important cause of recurrence. The aim of this study was to provide histological evidence of the 'neovascularisation'process.
    Method: Tissue samples from the region of the previously ligated saphenofemoral junction (SFJ) were taken from 14 limbs with recurrent varicose veins and from nine control limbs. Tissue samples were analysed histologically for overall vascularity, and the presence of intimal circular fibrosis, intimal eccentric fibrosis, medial thickened elastosis, and thrombosis in the microscopic thin walled vessels within the tissue. The same samples were analysed immunohistoligically for S100, a neural marker, and Ki-67 (Mib 1), a marker of endothelial proliferation. Absent S100 and positive Ki-67 were considered as evidence of new vessels.
    Result: No significant difference was found between the venous recurrence and control groups in respect to histological features. S100 positive nerve fibrils were seen associated with dilated venous channels in the majority of both redo and control groups (p=1, Fisher's exact test). Only one section stained positively with Ki-67 (Mib1) in a single vascular channel for a few endothelial cells. The remaining control and redo cases were negative for Mib 1 (p=1, Fisher's exact test).
    Conclusion: We found little evidence of neovascularisation associated with recurrent varicose veins in the saphenofemoral region. The venous channels that develop at the previously ligated SFJ may represent adaptive dilatation of pre-existing venous channels (vascular remodelling), probably in response to abnormal haemodynamic forces.
    MeSH term(s) Adult ; Aged ; Endothelium, Vascular/pathology ; Femoral Vein/pathology ; Humans ; Immunohistochemistry ; Ki-67 Antigen/metabolism ; Middle Aged ; Neovascularization, Pathologic ; Recurrence ; S100 Proteins/metabolism ; Saphenous Vein/pathology ; Varicose Veins/metabolism ; Varicose Veins/pathology
    Chemical Substances Ki-67 Antigen ; S100 Proteins
    Language English
    Publishing date 2004-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1225869-6
    ISSN 1532-2165 ; 1078-5884
    ISSN (online) 1532-2165
    ISSN 1078-5884
    DOI 10.1016/j.ejvs.2004.09.011
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  6. Book ; Online: Benchmarking of 3D space charge codes using direct phase space measurements from photoemission high voltage DC gun

    Bazarov, Ivan V. / Dunham, Bruce M. / Gulliford, Colwyn / Li, Yulin / Liu, Xianghong / Sinclair, Charles K. / Soong, Ken / Hannon, Fay

    2008  

    Abstract: We present a comparison between space charge calculations and direct measurements of the transverse phase space for space charge dominated electron bunches after a high voltage photoemission DC gun followed by an emittance compensation solenoid magnet. ... ...

    Abstract We present a comparison between space charge calculations and direct measurements of the transverse phase space for space charge dominated electron bunches after a high voltage photoemission DC gun followed by an emittance compensation solenoid magnet. The measurements were performed using a double-slit setup for a set of parameters such as charge per bunch and the solenoid current. The data is compared with detailed simulations using 3D space charge codes GPT and Parmela3D with initial particle distributions created from the measured transverse and temporal laser profiles. Beam brightness as a function of beam fraction is calculated for the measured phase space maps and found to approach the theoretical maximum set by the thermal energy and accelerating field at the photocathode.

    Comment: 11 pages, 23 figures. submitted to Phys Rev ST-AB
    Keywords Physics - Accelerator Physics
    Subject code 621
    Publishing date 2008-06-16
    Publishing country us
    Document type Book ; Online
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