LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Gums, Jeremiah J"
  2. AU="Petsalaki, Eleni"
  3. AU="Yu, Weichao"
  4. AU="Mertens, Anne Wiebke"
  5. AU="Roitershtein, Alexander"
  6. AU="Deppen, Stephen"
  7. AU="Goliath, Rene"
  8. AU="Emons, Günter"
  9. AU="Sarah S. Barnett"

Suchergebnis

Treffer 1 - 5 von insgesamt 5

Suchoptionen

  1. Artikel: Amiodarone-induced myxedema coma - a case and review of the literature.

    Chakraborty, Subhankar / Fedderson, Julie / Gums, Jeremiah J / Toole, Ashley

    Archives of medical science : AMS

    2013  Band 10, Heft 6, Seite(n) 1263–1267

    Sprache Englisch
    Erscheinungsdatum 2013-05-27
    Erscheinungsland Poland
    Dokumenttyp Journal Article
    ZDB-ID 2203781-0
    ISSN 1734-1922
    ISSN 1734-1922
    DOI 10.5114/aoms.2013.35026
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel: RNA interference in human foreskin fibroblasts within the three-dimensional collagen matrix.

    Carlson, Mark A / Prall, Amy K / Gums, Jeremiah J

    Molecular and cellular biochemistry

    2007  Band 306, Heft 1-2, Seite(n) 123–132

    Abstract: The technique of RNA interference (RNAi) was trialed in primary human foreskin fibroblasts, both in monolayer culture and in the fibroblast-populated collagen matrix. Knockdown of lamin A/C, p53, and FAK was possible with low-confluency (<50%) monolayer ... ...

    Abstract The technique of RNA interference (RNAi) was trialed in primary human foreskin fibroblasts, both in monolayer culture and in the fibroblast-populated collagen matrix. Knockdown of lamin A/C, p53, and FAK was possible with low-confluency (<50%) monolayer fibroblasts, a transfection vehicle concentration of 1%, and an siRNA concentration of 25-50 nM. Knockdown also was possible in the collagen matrix using similar reagent concentrations and a cellular density of one million fibroblasts per ml of matrix. Optimization of transfection conditions appeared to be important to increase knockdown efficiency. Consistent with prediction, knockdown of FAK induced apoptosis in the fibroblast-populated collagen matrix.
    Mesh-Begriff(e) Cells, Cultured ; Collagen/metabolism ; Extracellular Matrix/metabolism ; Fibroblasts/metabolism ; Fluorescent Antibody Technique ; Focal Adhesion Kinase 1/antagonists & inhibitors ; Focal Adhesion Kinase 1/genetics ; Focal Adhesion Kinase 1/metabolism ; Foreskin/cytology ; Humans ; Kidney/metabolism ; Lamin Type A/antagonists & inhibitors ; Lamin Type A/genetics ; Lamin Type A/metabolism ; Male ; RNA Interference ; RNA, Small Interfering/pharmacology ; Tumor Suppressor Protein p53/antagonists & inhibitors ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism
    Chemische Substanzen Lamin Type A ; RNA, Small Interfering ; Tumor Suppressor Protein p53 ; lamin C ; Collagen (9007-34-5) ; Focal Adhesion Kinase 1 (EC 2.7.10.2)
    Sprache Englisch
    Erscheinungsdatum 2007-12
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 184833-1
    ISSN 1573-4919 ; 0300-8177
    ISSN (online) 1573-4919
    ISSN 0300-8177
    DOI 10.1007/s11010-007-9561-z
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 892: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel ; Online: Biologic variability of human foreskin fibroblasts in 2D and 3D culture

    Lesiak Alex / Gums Jeremiah J / Prall Amy K / Carlson Mark A / Shostrom Valerie K

    BMC Research Notes, Vol 2, Iss 1, p

    implications for a wound healing model

    2009  Band 229

    Abstract: Abstract Background The fibroblast-populated 3D collagen matrix is a model of tissue and healing which has been used since the 1980's. It was hypothesized that anchorage disruption of the collagen matrix would produce p53-dependent apoptosis in the ... ...

    Abstract Abstract Background The fibroblast-populated 3D collagen matrix is a model of tissue and healing which has been used since the 1980's. It was hypothesized that anchorage disruption of the collagen matrix would produce p53-dependent apoptosis in the embedded fibroblasts, but results of hypothesis testing were variant. Findings The response of p53 to anchorage disruption in 3D culture or to UV irradiation in 2D culture was influenced both by fibroblast strain and culture conditions. It also was determined that data scatter in a collagen matrix contraction assay was related to fibroblast strain and possibly to technical factors, such as cell culture technician and/or number of matrices utilized. Subsequent analysis suggested that phenotypic drift and/or inter-strain genetic variability may have been responsible for the data scatter. In addition, several technical factors were identified that may have contributed to the scatter. Conclusion Experimentation with human foreskin fibroblasts in both 2D and 3D culture can produce variant data. The underlying cause of the data scatter appears to be partially due to the biologic variability of the fibroblast.
    Schlagwörter Medicine ; R ; Biology (General) ; QH301-705.5 ; Science (General) ; Q1-390
    Sprache Englisch
    Erscheinungsdatum 2009-11-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  4. Artikel ; Online: Biologic variability of human foreskin fibroblasts in 2D and 3D culture: implications for a wound healing model.

    Carlson, Mark A / Prall, Amy K / Gums, Jeremiah J / Lesiak, Alex / Shostrom, Valerie K

    BMC research notes

    2009  Band 2, Seite(n) 229

    Abstract: Background: The fibroblast-populated 3D collagen matrix is a model of tissue and healing which has been used since the 1980's. It was hypothesized that anchorage disruption of the collagen matrix would produce p53-dependent apoptosis in the embedded ... ...

    Abstract Background: The fibroblast-populated 3D collagen matrix is a model of tissue and healing which has been used since the 1980's. It was hypothesized that anchorage disruption of the collagen matrix would produce p53-dependent apoptosis in the embedded fibroblasts, but results of hypothesis testing were variant.
    Findings: The response of p53 to anchorage disruption in 3D culture or to UV irradiation in 2D culture was influenced both by fibroblast strain and culture conditions. It also was determined that data scatter in a collagen matrix contraction assay was related to fibroblast strain and possibly to technical factors, such as cell culture technician and/or number of matrices utilized. Subsequent analysis suggested that phenotypic drift and/or inter-strain genetic variability may have been responsible for the data scatter. In addition, several technical factors were identified that may have contributed to the scatter.
    Conclusion: Experimentation with human foreskin fibroblasts in both 2D and 3D culture can produce variant data. The underlying cause of the data scatter appears to be partially due to the biologic variability of the fibroblast.
    Sprache Englisch
    Erscheinungsdatum 2009-11-18
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2413336-X
    ISSN 1756-0500 ; 1756-0500
    ISSN (online) 1756-0500
    ISSN 1756-0500
    DOI 10.1186/1756-0500-2-229
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  5. Artikel: RNA interference in human foreskin fibroblasts within the three-dimensional collagen matrix

    Carlson, Mark A / Prall, Amy K / Gums, Jeremiah J

    Molecular and cellular biochemistry. 2007 Dec., v. 306, no. 1-2

    2007  

    Abstract: The technique of RNA interference (RNAi) was trialed in primary human foreskin fibroblasts, both in monolayer culture and in the fibroblast-populated collagen matrix. Knockdown of lamin A/C, p53, and FAK was possible with low-confluency (<50%) monolayer ... ...

    Abstract The technique of RNA interference (RNAi) was trialed in primary human foreskin fibroblasts, both in monolayer culture and in the fibroblast-populated collagen matrix. Knockdown of lamin A/C, p53, and FAK was possible with low-confluency (<50%) monolayer fibroblasts, a transfection vehicle concentration of 1%, and an siRNA concentration of 25-50 nM. Knockdown also was possible in the collagen matrix using similar reagent concentrations and a cellular density of one million fibroblasts per ml of matrix. Optimization of transfection conditions appeared to be important to increase knockdown efficiency. Consistent with prediction, knockdown of FAK induced apoptosis in the fibroblast-populated collagen matrix.
    Schlagwörter fibroblasts ; RNA interference ; collagen ; extracellular matrix
    Sprache Englisch
    Erscheinungsverlauf 2007-12
    Umfang p. 123-132.
    Verlag Springer US
    Erscheinungsort Boston
    Dokumenttyp Artikel
    ZDB-ID 184833-1
    ISSN 1573-4919 ; 0300-8177
    ISSN (online) 1573-4919
    ISSN 0300-8177
    DOI 10.1007/s11010-007-9561-z
    Datenquelle NAL Katalog (AGRICOLA)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Bonn

    Z 74/721: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Zum Seitenanfang