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  1. Article ; Online: SulfoxFluor-enabled deoxyazidation of alcohols with NaN

    Guo, Junkai / Wang, Xiu / Ni, Chuanfa / Wan, Xiaolong / Hu, Jinbo

    Nature communications

    2022  Volume 13, Issue 1, Page(s) 2752

    Abstract: Direct deoxyazidation of alcohols with ... ...

    Abstract Direct deoxyazidation of alcohols with NaN
    Language English
    Publishing date 2022-05-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-022-30132-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Expression and clinical significance of 3 kinds of circular RNA in systemic lupus erythematosus

    GUO Junkai1 / ZHAO Xingwang / NI Bing / YOU Yi

    Di-san junyi daxue xuebao, Vol 42, Iss 24, Pp 2387-

    2020  Volume 2393

    Abstract: Objective To explore the expression and significance of 3 circular RNAs (circRNA), that is, circADCY9, circGARS and circMCTP2, in the patients with systemic lupus erythematosus (SLE), so as to provide new ideas and directions for its clinical diagnosis ... ...

    Abstract Objective To explore the expression and significance of 3 circular RNAs (circRNA), that is, circADCY9, circGARS and circMCTP2, in the patients with systemic lupus erythematosus (SLE), so as to provide new ideas and directions for its clinical diagnosis and treatment. Methods Peripheral blood mononuclear cells (PBMCs) were extracted from the SLE patients (n=35) and healthy volunteers (n=25, control group). The expression levels of above 3 circRNAs were detected by real-time quantitative PCR (RT-qPCR), and their correlations with SLE Disease Activity Index (SLEDAI) were analyzed. The diagnostic value of the 3 circRNAs was evaluated by receiver operating characteristic (ROC) curve. Their stability and tolerance were detected using ribonuclease R (RNaseR) treatment, and the stability of circADCY9 was further verified by PCR analysis. Results ① The levels of circADCY9 and circGARS were up-regulated, while that of circMCTP2 was down-regulated (All P values were < 0.000 1) in the SLE patients than the healthy volunteers. ② In SLE patients, the expression levels of circADCY9 and circGARS were positively correlated with the score of SLEDAI, and negatively with the level of complement C3. By contrast, the circMCTP2 level was negatively correlated with SLEDAI score and positively with complement C3 level. ③ The area under the curve (AUC) for circADCY9, circGARS and circMCTP2 were 0.850 0, 0.826 7 and 0.895 6, respectively. ④ The expression levels of the 3 circRNAs remained steady before and after RNaseR treatment, and the result of PCR showed that the tolerance of circADCY9 to RNaseR was better than that of ADCY9 mRNA. Conclusion The expression of circADCY9, circGARS and circMCTP2 is significantly abnormal in the PBMCs of SLE patients, and shows good stability, which is correlated with the activity of SLE.
    Keywords systemic lupus erythematosus ; peripheral blood mononuclear cells ; circular rna ; Medicine (General) ; R5-920
    Subject code 610
    Language Chinese
    Publishing date 2020-12-01T00:00:00Z
    Publisher Editorial Office of Journal of Third Military Medical University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Role of long non-coding RNA RP11-288L9.1 in systemic lupus erythematosus and underlying mechanism

    ZHAO Chenglei / ZHAO Xingwang / GUO Junkai / WANG Juan / ZHANG Min / ZHANG Lian / YOU Yi

    Di-san junyi daxue xuebao, Vol 43, Iss 13, Pp 1204-

    2021  Volume 1211

    Abstract: Objective To investigate the role of long non-coding RNA (lncRNA) RP11-288L9.1 in systemic lupus erythematosus (SLE) and its potential mechanism. Methods Transcriptome sequencing (RNA-seq) was used to screen the differentially expressed lncRNAs in ... ...

    Abstract Objective To investigate the role of long non-coding RNA (lncRNA) RP11-288L9.1 in systemic lupus erythematosus (SLE) and its potential mechanism. Methods Transcriptome sequencing (RNA-seq) was used to screen the differentially expressed lncRNAs in peripheral blood mononuclear cells (PBMCs) of SLE patients, and the expression levels of these lncRNAs in 8 pairs of SLE patients and healthy controls were further verified by qRT-PCR. The knockdown as well as over-expression models of RP11-288L9.1 were constructed respectively by transfecting recombinant lentivirus into macrophages, and the transfection efficiency was determined subsequently. The subcellular distribution of RP11-288L9.1 was detected by fluorescence in situ hybridization (FISH) technique. Moreover, CCK-8 assay and Annexin V-FITC apoptosis detection kit were applied to examine the proliferation and apoptosis of macrophages in both models, and qRT-PCR was conducted to detect the expression of macrophage-related inflammatory cytokines, IL-1β, IL-6, IL-4, IL-10 and TGF-β1 after the transfection. Results Six differentially expressed lncRNAs were found (P < 0.05), in which RP11-288L9.1 was significantly up-regulated (P < 0.01), and mainly located in the cytoplasm of macrophages. The over-expression of RP11-288L9.1 promoted the apoptosis and inhibited the proliferation of macrophages (P < 0.05), increasing the levels of IL-1β and IL-6, and decreasing those of IL-4, IL-10 and TGF-β1 (P < 0.01). In contrast, the knockdown of RP11-288L9.1 significantly induced the proliferation of macrophages and inhibited the apoptosis (P < 0.05), with lowered levels of IL-1β and IL-6 and elevated IL-4, IL-10 and TGF-β1 (P < 0.01). Conclusion RP11-288L9.1 is highly expressed in the PBMCs of SLE patients, and regulates immune response in macrophages by affecting the synthesis of inflammatory cytokines, which may contribute to the occurrence of SLE.
    Keywords systemic lupus erythematosus ; long non-coding rna ; rp11-288l9.1 ; macrophages ; Medicine (General) ; R5-920
    Subject code 610
    Language Chinese
    Publishing date 2021-07-01T00:00:00Z
    Publisher Editorial Office of Journal of Third Military Medical University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: N6-methyladenosine-dependent modification of circGARS acts as a new player that promotes SLE progression through the NF-κB/A20 axis.

    Zhao, Xingwang / Dong, Rui / Zhang, Longlong / Guo, Junkai / Shi, Ying / Ge, Lan / Wang, Juan / Song, Zhiqiang / Ni, Bing / You, Yi

    Arthritis research & therapy

    2022  Volume 24, Issue 1, Page(s) 37

    Abstract: Background: Certain circRNAs could be used as biomarkers to determine the risk of development and/or severity of systemic lupus erythematosus, and their new function in the regulation of gene expression has motivated us to investigate their role in SLE ... ...

    Abstract Background: Certain circRNAs could be used as biomarkers to determine the risk of development and/or severity of systemic lupus erythematosus, and their new function in the regulation of gene expression has motivated us to investigate their role in SLE METHODS: Experimental methods including qRT-PCR, RNA immunoprecipitation (RIP), pulldown, dual luciferase reporter assay, RNA interference and cell transfection, RNA fluorescence in situ hybridization, western blotting, and mass spectrometry were used to assessed circGARS (hsa_circRNA_0009000) for immune functions and defined mechanisms by which circGARS promotes the progression in SLE.
    Results: Our results demonstrated that the levels of circGARS was remarkably upregulated in SLE and correlated with clinicopathological features. CircGARS directly combined with microRNA-19a (miR-19a). Functionally, circGARS downregulated the expression of TNFAIP3 (A20, tumor necrosis factor alpha-induced protein 3) to mediate the activation of immune responses that were regulated by the nuclear factor-κB (NF-κB) pathway as a negative feedback mechanism. In addition, miR-19a regulated A20 (TNFAIP3) degradation by downregulating the expression of YTH N6-methyladenosine RNA-binding protein 2 (YTHDF2).
    Conclusions: The circGARS sponges miR-19a to regulate YTHDF2 expression to promote SLE progression through the A20/NF-κB axis and may act as an independent biomarker to help the treatment of SLE patients.
    MeSH term(s) Adenosine/analogs & derivatives ; Humans ; In Situ Hybridization, Fluorescence ; Lupus Erythematosus, Systemic/genetics ; NF-kappa B/metabolism ; RNA, Circular/genetics ; Tumor Necrosis Factor alpha-Induced Protein 3/genetics ; Tumor Necrosis Factor alpha-Induced Protein 3/metabolism
    Chemical Substances NF-kappa B ; RNA, Circular ; N-methyladenosine (CLE6G00625) ; TNFAIP3 protein, human (EC 3.4.19.12) ; Tumor Necrosis Factor alpha-Induced Protein 3 (EC 3.4.19.12) ; Adenosine (K72T3FS567)
    Language English
    Publishing date 2022-02-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2107602-9
    ISSN 1478-6362 ; 1478-6354
    ISSN (online) 1478-6362
    ISSN 1478-6354
    DOI 10.1186/s13075-022-02732-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Integrated Transcriptome Profiling Revealed That Elevated Long Non-Coding RNA-

    You, Yi / Zhao, Xingwang / Wu, Yaguang / Mao, Jiangming / Ge, Lan / Guo, Junkai / Zhao, Chenglei / Chen, Dong / Song, Zhiqiang

    Frontiers in immunology

    2021  Volume 12, Page(s) 615859

    Abstract: Purpose: Systemic lupus erythematosus (SLE) is a serious autoimmune disease. Its molecular pathogenesis, especially the long non-coding RNA (lncRNA) function, remains unclear. We want to investigate the lncRNA dysregulation profile and their molecular ... ...

    Abstract Purpose: Systemic lupus erythematosus (SLE) is a serious autoimmune disease. Its molecular pathogenesis, especially the long non-coding RNA (lncRNA) function, remains unclear. We want to investigate the lncRNA dysregulation profile and their molecular mechanisms in SLE.
    Methods: In this study, we analyzed the transcriptome profiles (RNA-seq) of peripheral blood mononuclear cells (PBMCs) from SLE patients and two published transcriptome datasets to explore lncRNA profiles. The differentially expressed lncRNAs were confirmed by quantitative real-time PCR in another set of female patients. We constructed the lncRNA-mRNA regulatory networks by performing weighted gene co-expression network analysis (WGCNA). Dysregulated lncRNA AC007278.2 was repressed by short hairpin RNA (shRNA) in Jurkat cells. Dual-luciferase reporter gene assay was performed to investigate the regulatory mechanism of AC007278.2 on target gene CCR7.
    Results: We observed dominant up-regulation of transcripts, including mRNAs and lncRNAs, in SLE patients. By WGCNA method, we identified three modules that were highly related to SLE. We then focused on one lncRNA,
    Conclusion: In summary, our study indicated the important regulatory role of lncRNAs in SLE.
    MeSH term(s) Autoimmunity/genetics ; Cell Differentiation ; Gene Expression Profiling ; Gene Expression Regulation ; Genetic Markers/genetics ; Germinal Center/immunology ; Humans ; Jurkat Cells ; Lupus Erythematosus, Systemic/diagnosis ; Lupus Erythematosus, Systemic/genetics ; RNA, Long Noncoding/genetics ; RNA, Small Interfering/genetics ; Receptors, CCR7/genetics ; Receptors, CCR7/metabolism ; Th1 Cells/physiology ; Transcriptome ; Up-Regulation
    Chemical Substances CCR7 protein, human ; Genetic Markers ; RNA, Long Noncoding ; RNA, Small Interfering ; Receptors, CCR7
    Language English
    Publishing date 2021-06-16
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.615859
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  6. Article ; Online: Rapid Deoxyfluorination of Alcohols with N-Tosyl-4-chlorobenzenesulfonimidoyl Fluoride (SulfoxFluor) at Room Temperature.

    Guo, Junkai / Kuang, Cuiwen / Rong, Jian / Li, Lingchun / Ni, Chuanfa / Hu, Jinbo

    Chemistry (Weinheim an der Bergstrasse, Germany)

    2019  Volume 25, Issue 30, Page(s) 7259–7264

    Abstract: The deoxyfluorination of alcohols is a fundamentally important approach to access alkyl fluorides, and thus the development of shelf-stable, easy-to-handle, fluorine-economical, and highly selective deoxyfluorination reagents is highly desired. This work ...

    Abstract The deoxyfluorination of alcohols is a fundamentally important approach to access alkyl fluorides, and thus the development of shelf-stable, easy-to-handle, fluorine-economical, and highly selective deoxyfluorination reagents is highly desired. This work describes the development of a crystalline compound, N-tosyl-4-chlorobenzenesulfonimidoyl fluoride (SulfoxFluor), as a novel deoxyfluorination reagent that possesses all of the aforementioned merits, which is rare in the arena of deoxyfluorination. Endowed by the multi-dimensional modulating ability of the sulfonimidoyl group, SulfoxFluor is superior to 2-pyridinesulfonyl fluoride (PyFluor) in fluorination rate, and is also superior to perfluorobutanesulfonyl fluoride (PBSF) in fluorine-economy. Its reaction with alcohols not only tolerates a wide range of functionalities including the more sterically hindered alcoholic hydroxyl groups, but also exhibits high fluorination/elimination selectivity. Because SulfoxFluor can be easily prepared from inexpensive materials and can be safely handled without special techniques, it promises to serve as a practical deoxyfluorination reagent for the synthesis of various alkyl fluorides.
    Language English
    Publishing date 2019-04-26
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1478547-x
    ISSN 1521-3765 ; 0947-6539
    ISSN (online) 1521-3765
    ISSN 0947-6539
    DOI 10.1002/chem.201901176
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  7. Article: Radical (Phenylsulfonyl)difluoromethylation of Isocyanides with PhSO2CF2H under Transition-Metal-Free Conditions

    Xiao, Pan / Rong Jian / Ni Chuanfa / Guo Junkai / Li Xinjin / Chen Dingben / Hu Jinbo

    Organic letters. 2016 Nov. 18, v. 18, no. 22

    2016  

    Abstract: An atom-economical method for radical (phenylsulfonyl)difluoromethylation of isocyanides with PhSO₂CF₂H under transition-metal-free conditions has been developed. A PhSO₂CF₂ radical is generated through the oxidation of PhSO₂CF₂– after the ... ...

    Abstract An atom-economical method for radical (phenylsulfonyl)difluoromethylation of isocyanides with PhSO₂CF₂H under transition-metal-free conditions has been developed. A PhSO₂CF₂ radical is generated through the oxidation of PhSO₂CF₂– after the deprotonation of PhSO₂CF₂H in one pot. The reaction exhibits excellent functional-group tolerance and the resulting products can be further modified with the removal of a PhSO₂ group to give other CF₂-containing compounds.
    Keywords chemical structure ; organic compounds ; oxidation
    Language English
    Dates of publication 2016-1118
    Size p. 5912-5915.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1523-7052
    DOI 10.1021%2Facs.orglett.6b03013
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Radical (Phenylsulfonyl)difluoromethylation of Isocyanides with PhSO

    Xiao, Pan / Rong, Jian / Ni, Chuanfa / Guo, Junkai / Li, Xinjin / Chen, Dingben / Hu, Jinbo

    Organic letters

    2016  Volume 18, Issue 22, Page(s) 5912–5915

    Abstract: An atom-economical method for radical (phenylsulfonyl)difluoromethylation of isocyanides with ... ...

    Abstract An atom-economical method for radical (phenylsulfonyl)difluoromethylation of isocyanides with PhSO
    Language English
    Publishing date 2016-11-18
    Publishing country United States
    Document type Journal Article
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.6b03013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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