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  1. Article ; Online: The Influence of GFRαl Inhibition on Proliferation and Apoptosis of Spermatogenic Cells

    Mao, Jianmin / Liu, Peng / Han, Wenjun / Mo, Ran / Guo, Shishuai / Sun, Jianming

    Cytol. Genet.. 2022 Dec., v. 56, no. 6 p.567-573

    2022  

    Abstract: The role of the GFRαl receptor in the regulation of cell differentiation and spermatogenesis is poorly investigated. The influence of GFRα1 on the apoptosis and proliferation of spermatogenic cells are not fully elucidated. In this study, GC-1 spg cells ... ...

    Abstract The role of the GFRαl receptor in the regulation of cell differentiation and spermatogenesis is poorly investigated. The influence of GFRα1 on the apoptosis and proliferation of spermatogenic cells are not fully elucidated. In this study, GC-1 spg cells were cultured and then assigned to a control cell (control group) transfected with empty adenovirus AdH1-siRNA and an inhibited cells (inhibited group) with GFRα1 interfering adenovirus (AdH1-siRNA/GFRα1). The GFRal inhibition was determined by employing western blot and qRT-PCR analysis. Furthermore, we used CCK8 for determining the proliferation of the GFRα1 interfering adenovirus (AdH1-siRNA/GFRα1). Transfected cells, employing flow cytometry and Caspase 3, Bax and Bcl-2 expression analysis to determine their apoptosis. This study demonstrated that after transfection, the inhibited cells presented lower proliferation and invasion and higher apoptosis. According to these findings, the inhibition of GFRαl might reduce spermatogenesis and remarkably affect spermatogenic cells proliferation.
    Keywords Western blotting ; apoptosis ; caspase-3 ; cell differentiation ; flow cytometry ; spermatogenesis ; transfection
    Language English
    Dates of publication 2022-12
    Size p. 567-573.
    Publishing place Pleiades Publishing
    Document type Article ; Online
    ZDB-ID 390717-x
    ISSN 1934-9440 ; 0095-4527
    ISSN (online) 1934-9440
    ISSN 0095-4527
    DOI 10.3103/S0095452722060081
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2982: Show issues

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  2. Article: Sheng Jing Decoction Can Promote Spermatogenesis and Increase Sperm Motility of the Oligozoospermia Mouse Model.

    Yan, Guang / Tian, Fang / Liu, Peng / Sun, Jianming / Mao, Jianmin / Han, Wenjun / Mo, Ran / Guo, Shishuai / Yu, Quanyao

    Evidence-based complementary and alternative medicine : eCAM

    2021  Volume 2021, Page(s) 3686494

    Abstract: Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the ... ...

    Abstract Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the functions of SJD on spermatogenesis and sperm motility and explored the potential mechanisms involved. Here, we demonstrated that high, medium, and low doses of SJD are effective in restoring the impairments of the whole body and testicular tissue by cyclophosphamide inducing and to rescue the damage of testicular tissue cells including Sertoli cells and germ cells. SJD can partly restore the decrease in sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate in oligozoospermic mouse models. Ki67 staining analyses confirm SJD can promote testicular tissue cell proliferation. Real-time RT-PCR analyses also reveal that SJD can upregulate the expression of proliferation-associated gene
    Language English
    Publishing date 2021-12-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2021/3686494
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5995: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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