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  1. Article ; Online: Integrating network pharmacology and experimental validation to decipher the mechanism of the Chinese herbal prescription modified Shen-Yan-Fang-Shuai formula in treating diabetic nephropathy

    Borui Yu / Mengqi Zhou / Zhaocheng Dong / Huijuan Zheng / Yuxue Zhao / Jingwei Zhou / Chao Zhang / Fudong Wei / Guoyong Yu / Wei Jing Liu / Hongfang Liu / Yaoxian Wang

    Pharmaceutical Biology, Vol 61, Iss 1, Pp 1222-

    2023  Volume 1233

    Abstract: AbstractContext Diabetic nephropathy (DN) is the main cause of end-stage renal disease. Modified Shen-Yan-Fang-Shuai formula (M-SYFSF) has excellent clinical efficacy in treating diabetic kidney disease. However, the potential mechanism of M-SYFSF ... ...

    Abstract AbstractContext Diabetic nephropathy (DN) is the main cause of end-stage renal disease. Modified Shen-Yan-Fang-Shuai formula (M-SYFSF) has excellent clinical efficacy in treating diabetic kidney disease. However, the potential mechanism of M-SYFSF remains unknown.Objective To investigate the mechanism of M-SYFSF against DN by network pharmacological analysis and biological experiments.Materials and methods Utilizing a web-based pharmacology database, the potential mechanisms of M-SYFSF against DN were identified. In vivo experiments, male SD rats were injected with streptozotocin (50 mg/kg) and got uninephrectomy to construct a model of DN. M-SYFSF (11.34 g/kg/d) was gavaged once per day for 12 weeks after model establishment. In vitro experiments, human proximal tubular cells (HK-2) were performed with advanced glycation end-products (AGEs) (100 μg/mL), then intervened with M-SYFSF freeze-dried powder. Pathological staining, WB, IHC, ELISA were conducted to explore the mechanism of M-SYFSF against DN.Results Network pharmacological analysis showed that MAPK pathway was the potential pathway. Results showed that compared with the Model group, M-SYFSF significantly reduced 24h urine albumin, UACR, and serum creatinine levels (54.90 ± 26.67 vs. 111.78 ± 4.28, 8.87 ± 1.69 vs. 53.94 ± 16.01, 11.56 ± 1.70 vs. 118.70 ± 49.57, respectively), and improved renal pathological changes. Furthermore, the intervention of M-SYFSF reduced the expression of pro-inflammatory cytokines and inhibited the activation of MAPK pathway in AGEs-treated HK-2 cells.Discussion and conclusion M-SYFSF is likely to reduce inflammation in DN by inhibiting the MAPK pathway. It provides a theoretical basis for the clinical application of M-SYFSF in the treatment of DN.
    Keywords Traditional Chinese medicine formula ; protein-protein interaction network ; MAPK signaling pathway ; inflammation ; Therapeutics. Pharmacology ; RM1-950
    Subject code 616
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Pumpkin polysaccharide modifies the gut microbiota during alleviation of type 2 diabetes in rats

    Liu, Guimei / Li Liang / Guoyong Yu / Quanhong Li

    International journal of biological macromolecules. 2018 Aug., v. 115

    2018  

    Abstract: Pumpkin polysaccharide is able to alleviate diabetes, but understanding of the underlining mechanism is still limited. In this study, we hypothesized that the alleviating effects of pumpkin polysaccharide is modulated via changes in the gut microbiota ... ...

    Abstract Pumpkin polysaccharide is able to alleviate diabetes, but understanding of the underlining mechanism is still limited. In this study, we hypothesized that the alleviating effects of pumpkin polysaccharide is modulated via changes in the gut microbiota and short-chain fatty acid (SCFA) production in type 2 diabetic rats. After the type 2 diabetic model successfully was established, three groups of high-fat diet induced diabetic rats were intragastrically administered pumpkin polysaccharide, metformin, or saline solution respectively. We utilized 16S rRNA gene sequencing and multivariate statistics to analyze the structural and key species of gut microbiota in the type 2 diabetic rats. The results revealed that pumpkin polysaccharide alleviated the type 2 diabetes by improving the insulin tolerance and decreasing the levels of serum glucose (GLU), total cholesterol (TC), and low-density lipoprotein (LDL-C), while increasing the levels of high-density lipoprotein (HDL-C). Simultaneously, pumpkin polysaccharide changed the structure of gut microbiota and had selective enrichment in key species of Bacteroidetes, Prevotella, Deltaproteobacteria, Oscillospira, Veillonellaceae, Phascolarctobacterium, Sutterella, and Bilophila. The correlations between the key species and SCFA production indicated the underlining mechanisms of pumpkin polysaccharide on type 2 diabetes.
    Keywords Bilophila ; Oscillospira ; Phascolarctobacterium ; Prevotella ; Sutterella ; Veillonellaceae ; animal disease models ; blood glucose ; cholesterol ; high density lipoprotein ; high fat diet ; insulin resistance ; intestinal microorganisms ; low density lipoprotein ; metformin ; multivariate analysis ; noninsulin-dependent diabetes mellitus ; polysaccharides ; pumpkins ; rats ; ribosomal RNA ; sequence analysis ; short chain fatty acids ; sodium chloride
    Language English
    Dates of publication 2018-08
    Size p. 711-717.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2018.04.127
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    Zs.B 2374: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  3. Article: Stachyose Improves Inflammation through Modulating Gut Microbiota of High‐Fat Diet/Streptozotocin‐Induced Type 2 Diabetes in Rats

    Liu, Guimei / Guoyong Yu / Jia Bei / Li Liang / Lu Li / Quanhong Li

    Molecular nutrition & food research. 2018 Mar., v. 62, no. 6

    2018  

    Abstract: SCOPE: The present study is undertaken to assess the effects of stachyose (STS) on type 2 diabetes in rats and changes in the gut microbiota compared to metformin (MET). METHODS AND RESULTS: The type 2 diabetic model is successfully established via a ... ...

    Abstract SCOPE: The present study is undertaken to assess the effects of stachyose (STS) on type 2 diabetes in rats and changes in the gut microbiota compared to metformin (MET). METHODS AND RESULTS: The type 2 diabetic model is successfully established via a high‐fat diet /streptozotocin in Wistar rats, and STS or MET is administered for 4 weeks. Blood is collected to analyze biochemical parameters, pancreas for mRNA expression of related gene, and contents of colon for gut microbiota. STS or MET decreases serum LPS, mRNA expression of IL‐6, and tumor necrosis factor‐α (TNF‐α). In addition, STS and MET show a similar shifting of the structure of the gut microbiota and a selective enrichment of key species. At the genus level, STS shows selective enrichment of Phascolarctobacterium, Bilophila, Oscillospira, Turicibacter, and SMB5, but MET demonstrates a selective effect on Sutterella, Prevotella, 02d06, and rc4. The correlation analysis indicates that STS and MET decrease IL‐6 and TNF‐α and increase Akt/PI3K expression, which are relative to key species of gut microbiota. CONCLUSION: STS decreases pancreatic mRNA expression of IL‐6 and TNF‐α via key species of gut microbiota. The mechanism of this effect is similar to that of MET.
    Keywords Bilophila ; blood serum ; colon ; correlation ; gene expression ; genes ; high fat diet ; inflammation ; interleukin-6 ; intestinal microorganisms ; messenger RNA ; metformin ; models ; noninsulin-dependent diabetes mellitus ; Oscillospira ; pancreas ; Phascolarctobacterium ; Prevotella ; rats ; stachyose ; Sutterella ; tumor necrosis factor-alpha
    Language English
    Dates of publication 2018-03
    Size p. e1700954.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2160372-8
    ISSN 1613-4133 ; 1613-4125
    ISSN (online) 1613-4133
    ISSN 1613-4125
    DOI 10.1002/mnfr.201700954
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  4. Article ; Online: Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma

    Guoyong Yu / Zhaotao Wang / Shulian Zeng / Sisi Liu / Chunping Zhu / Ruxiang Xu / Ru-en Liu

    BioMed Research International, Vol

    2019  Volume 2019

    Abstract: Paeoniflorin (PF), as one of the important valid natural compounds of the total glucosides of peony, has displayed a potential effect in cancer prevention and treatment. Aggressive migration and invasion, as an important process, can contribute to tumor ... ...

    Abstract Paeoniflorin (PF), as one of the important valid natural compounds of the total glucosides of peony, has displayed a potential effect in cancer prevention and treatment. Aggressive migration and invasion, as an important process, can contribute to tumor progression through infiltrating the surround normal tissue. Actin cytoskeleton rearrangement plays a key role in cells migration and invasion, involving multiple signal pathways. HGF/c-Met signal, as an important couple of oncoprotein, has been demonstrated to regulate actin cytoskeleton rearrangement. In our study, we aim to explore whether paeoniflorin can inhibit migration and invasion and actin cytoskeleton rearrangement via regulation of HGF/c-Met/RhoA/ROCK signal. Various approaches were applied to demonstrate the mechanism of paeoniflorin-mediated anticancer effect, including cell wound healing assay, invasion assay, immunofluorescence staining and transfection, and western blotting. We observed that paeoniflorin inhibited HGF-induced migration and invasion and actin cytoskeleton rearrangement in glioblastoma cells. Furthermore, the inhibition of HGF-induced migration and invasion and actin cytoskeleton rearrangement involved c-Met-mediated RhoA/ROCK signaling in glioblastoma. Thus, our study proved that paeoniflorin could inhibit migration and invasion and actin cytoskeleton rearrangement through inhibition of HGF/c-Met/RhoA/ROCK signaling in glioblastoma, suggesting that paeoniflorin might be a candidate compound to treat glioblastoma.
    Keywords Medicine ; R
    Subject code 572
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Alditols and monosaccharides from sorghum vinegar can attenuate platelet aggregation by inhibiting cyclooxygenase-1 and thromboxane-A2 synthase

    Li, Jing / Guoyong Yu / Junfeng Fan

    Journal of ethnopharmacology. 2014 Aug. 08, v. 155, no. 1

    2014  

    Abstract: Vinegar has been used as both a common seasoning and a traditional Chinese medicine. Sorghum vinegar is an excellent source of physiological substances with multiple health benefits.To evaluate the antiplatelet aggregation activity of alditols and ... ...

    Abstract Vinegar has been used as both a common seasoning and a traditional Chinese medicine. Sorghum vinegar is an excellent source of physiological substances with multiple health benefits.To evaluate the antiplatelet aggregation activity of alditols and monosaccharides extracted from sorghum vinegar and analysis its mechanism.Alditol and monosaccharide extract (AME) from sorghum vinegar was first evaluated for antiplatelet activity using the turbidimetric method. Blood was collected from healthy volunteer donors. The platelet aggregation was induced by arachidonic acid (AA), collagen, adenosine diphosphate (ADP) and thrombin in vitro. AME was divided into three experimental groups with the concentration were 0.10, 0.25 and 0.50mg/mL. In order to determine the inhibitory activity of AME on COX1, TXS and TXA2 production experiments were conducted using the COX1, TXS and TXB2 EIA kit. Computational docking was used to find the docking pose of monosaccharides and alditols with COX1.AME showed significant induction of antiplatelet activity by arachidonic acid (AA), collagen, adenosine diphosphate (ADP) and thrombin in a concentration-dependent manner (p<0.05). AME (0.50mg/mL) reduced the AA-induced aggregation rate to 10.35%±0.46%, which was comparable to acetylsalicylic acid (aspirin, ASA) (0.50mg/mL, 6.35%±0.58%), a medical standard. Furthermore, AME strongly inhibited cyclooxygenase-1 (COX1) and thromboxane-A2 synthase (TXS), and subsequently attenuated thromboxane-A2 (TXA2) production. These findings indicated that AME attenuates platelet aggregation through the AA metabolism pathway. Computational docking showed that alditols (L-erythritol, L-arabitol, xylitol and D-sorbitol), monosaccharides (D-glucopyranose, D-fructofuranonse, D-xylopyranose, D-galactopyranose and D-ribose), ethyl glucoside and 3,4-(methylenedioxy) mandelic acid could dock directly into the active site of COX1.Alditols and monosaccharides from sorghum vinegar inhibit multiple steps in the platelet aggregation pathway, and may be beneficial for the treatment of cardiovascular diseases.
    Keywords active sites ; adenosine diphosphate ; arachidonic acid ; aspirin ; blood ; cardiovascular diseases ; collagen ; glucosides ; mandelic acid ; metabolism ; monosaccharides ; Oriental traditional medicine ; platelet aggregation ; prostaglandin synthase ; sorbitol ; Sorghum (Poaceae) ; thrombin ; vinegars ; xylitol
    Language English
    Dates of publication 2014-0808
    Size p. 285-292.
    Publishing place Elsevier Ireland Ltd
    Document type Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2014.05.018
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    Z 90/710: Show issues

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  6. Article: Dipeptidyl Peptidase IV Inhibitory Peptides Derived from Oat (Avena sativa L.), Buckwheat (Fagopyrum esculentum), and Highland Barley (Hordeum vulgare trifurcatum (L.) Trofim) Proteins

    Wang, Feng / Guoyong Yu / Yanyan Zhang / Bolin Zhang / Junfeng Fan

    Journal of agricultural and food chemistry. 2015 Nov. 4, v. 63, no. 43

    2015  

    Abstract: Peptides released from oat, buckwheat, and highland barley proteins were examined for their in vitro inhibitory effects on dipeptidyl peptidase IV (DPP4), an enzyme that deactivates incretin hormones involved in insulin secretion. All of the hydrolysates ...

    Abstract Peptides released from oat, buckwheat, and highland barley proteins were examined for their in vitro inhibitory effects on dipeptidyl peptidase IV (DPP4), an enzyme that deactivates incretin hormones involved in insulin secretion. All of the hydrolysates exhibited DPP4 inhibitory activities, with IC50 values ranging from 0.13 mg/mL (oat glutelin alcalase digestion) to 8.15 mg/mL (highland barley albumin tryptic digestion). The lowest IC50 values in gastrointestinal, alcalase, and tryptic digestions were 0.99 mg/mL (oat flour), 0.13 mg/mL (oat glutelin), and 1.83 mg/mL (highland barley glutelin). In all, 35 peptides of more than seven residues were identified in the tryptic hydrolysates of oat globulin using liquid chromatography–mass spectroscopy. Peptides LQAFEPLR and EFLLAGNNK were synthesized and their DPP4 inhibitory activities determined. LQAFEPLR showed high in vitro DPP4 inhibitory activity with an IC50 value of 103.5 μM.
    Keywords Avena sativa ; Fagopyrum esculentum ; Hordeum vulgare ; albumins ; barley ; barley protein ; buckwheat ; digestion ; dipeptidyl-peptidase IV ; gastrointestinal system ; globulins ; glutelins ; hydrolysates ; inhibitory concentration 50 ; insulin secretion ; liquid chromatography ; oat flour ; oats ; secretin ; spectroscopy ; subtilisin
    Language English
    Dates of publication 2015-1104
    Size p. 9543-9549.
    Publishing place American Chemical Society, Books and Journals Division
    Document type Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.5b04016
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    Z 1185: Show issues

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