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Article ; Online: Impelling TLR9: Road to perspective vaccine for visceral leishmaniasis.

Tiwari, Rohit Kumar / Gupta, Chhedi Lal / Bajpai, Preeti

2020 Volume 83, Issue 2, Page(s) 222–224

Abstract: Recent trends in immunotherapy have shown enthusiasm in exploring Toll-like receptors (TLRs) for designing therapeutical interventions against numerous deadly diseases. TLRs are subfamily of pathogen recognition receptor playing pivotal role in innate ... ...

Abstract	Recent trends in immunotherapy have shown enthusiasm in exploring Toll-like receptors (TLRs) for designing therapeutical interventions against numerous deadly diseases. TLRs are subfamily of pathogen recognition receptor playing pivotal role in innate immunity. TLR9 is one such critical member belonging to intracellular TLRs which is associated with mounting inflammatory response in response to intruders. Explorative studies have shown CG motifs from the prokaryotic origin as activators of TLR9 culminating in the expression of NFκB. These CG rich short stranded DNA sequences have been further delineated into different classes based on their structural specificities and immunomodulatory properties. Here we discuss the progress of how activation of TLR9 can be utilized with novel parasitic CpG islands to function as potential adjuvants specifically against protozoan parasitic diseases primarily visceral leishmaniasis caused by Leishmania donovani.
MeSH term(s)	CpG Islands ; Humans ; Leishmania donovani/genetics ; Leishmania donovani/metabolism ; Leishmaniasis, Visceral/prevention & control ; Toll-Like Receptor 9/genetics ; Toll-Like Receptor 9/metabolism ; Vaccines
Chemical Substances	TLR9 protein, human ; Toll-Like Receptor 9 ; Vaccines
Language	English
Publishing date	2020-03-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	604587-x
ISSN	1098-2299 ; 0272-4391
ISSN (online)	1098-2299
ISSN	0272-4391
DOI	10.1002/ddr.21662
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Zs.B 2542: Show issues

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Article: Comparative genomics of

Moshe, Maya / Gupta, Chhedi Lal / Sela, Noa / Minz, Dror / Banin, Ehud / Frenkel, Omer / Cytryn, Eddie

Frontiers in microbiology

2023 Volume 14, Page(s) 996287

Abstract: Bacillus cereus sensu ... ...

Abstract	Bacillus cereus sensu lato
Language	English
Publishing date	2023-02-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.996287
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Article: Corrigendum: Comparative genomics of

Moshe, Maya / Gupta, Chhedi Lal / Jain, Rakeshkumar Manojkumar / Sela, Noa / Minz, Dror / Banin, Ehud / Frenkel, Omer / Cytryn, Eddie

Frontiers in microbiology

2023 Volume 14, Page(s) 1271554

Abstract: This corrects the article DOI: 10.3389/fmicb.2023.996287.]. ...

Abstract	[This corrects the article DOI: 10.3389/fmicb.2023.996287.].
Language	English
Publishing date	2023-10-23
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1271554
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Article ; Online: Microglial TLR9: Plausible Novel Target for Therapeutic Regime Against Glioblastoma Multiforme.

Tiwari, Rohit Kumar / Singh, Sarika / Gupta, Chhedi Lal / Bajpai, Preeti

Cellular and molecular neurobiology

2020 Volume 41, Issue 7, Page(s) 1391–1393

Abstract: An understanding of pattern recognition receptors (PRRs) and immunomodulatory approach based on activation of these receptors has provided insights critical for the management of neurological health disorders. Toll-like receptors (TLRs) are one of the ... ...

Abstract	An understanding of pattern recognition receptors (PRRs) and immunomodulatory approach based on activation of these receptors has provided insights critical for the management of neurological health disorders. Toll-like receptors (TLRs) are one of the most widely explored PRRs and have been exploited in the recent past for development of novel immunomodulatory therapeutic agents. Glioblastoma multiforme is characterized by significant infiltration of resident microglia and expresses all the members of the TLR family. The present report is focused on exciting findings pertaining to probable implications of TLR9 activation by unmethylated CG sequences for novel therapeutic intervention against glioblastoma multiforme, which could be a discrete step toward the effective management of neurological health issues.
MeSH term(s)	Animals ; Glioblastoma/drug therapy ; Glioblastoma/metabolism ; Humans ; Microglia/drug effects ; Microglia/metabolism ; Receptors, Pattern Recognition/metabolism ; Signal Transduction/drug effects ; Toll-Like Receptor 9/genetics ; Toll-Like Receptor 9/metabolism
Chemical Substances	Receptors, Pattern Recognition ; TLR9 protein, human ; Toll-Like Receptor 9
Language	English
Publishing date	2020-07-20
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	283404-2
ISSN	1573-6830 ; 0272-4340
ISSN (online)	1573-6830
ISSN	0272-4340
DOI	10.1007/s10571-020-00925-z
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Zs.A 1727: Show issues

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Article ; Online: Hidden Resistome: Enrichment Reveals the Presence of Clinically Relevant Antibiotic Resistance Determinants in Treated Wastewater-Irrigated Soils.

Marano, Roberto B M / Gupta, Chhedi Lal / Cozer, Tamar / Jurkevitch, Edouard / Cytryn, Eddie

Environmental science & technology

2021 Volume 55, Issue 10, Page(s) 6814–6827

Abstract: Treated-wastewater (TW) irrigation transfers antibiotic-resistant bacteria (ARB) to soil, but persistence of these bacteria is generally low due to resilience of the soil microbiome. Nonetheless, wastewater-derived bacteria and associated antibiotic ... ...

Abstract	Treated-wastewater (TW) irrigation transfers antibiotic-resistant bacteria (ARB) to soil, but persistence of these bacteria is generally low due to resilience of the soil microbiome. Nonetheless, wastewater-derived bacteria and associated antibiotic resistance genes (ARGs) may persist below detection levels and potentially proliferate under copiotrophic conditions. To test this hypothesis, we exposed soils from microcosm, lysimeter, and field experiments to short-term enrichment in copiotroph-stimulating media. In microcosms, enrichment stimulated growth of multidrug-resistant
MeSH term(s)	Agricultural Irrigation ; Angiotensin Receptor Antagonists ; Angiotensin-Converting Enzyme Inhibitors ; Anti-Bacterial Agents/pharmacology ; Drug Resistance, Microbial/genetics ; Genes, Bacterial ; Humans ; Soil ; Soil Microbiology ; Waste Water/analysis
Chemical Substances	Angiotensin Receptor Antagonists ; Angiotensin-Converting Enzyme Inhibitors ; Anti-Bacterial Agents ; Soil ; Waste Water
Language	English
Publishing date	2021-04-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.1c00612
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Article ; Online: Molecular characterization of recombinant arginase of Leishmania donovani.

Gaur, Neha / Kumar, Kishore / Gupta, Chhedi Lal / Saxena, Jitendra Kumar

Protein expression and purification

2019 Volume 159, Page(s) 1–9

Abstract: Arginase catalyzes the first committed step in the biosynthesis of polyamines that enable cell growth and hence potential drug target for the treatment of leishmaniasis. The arginase from Leishmania donovani (LdARG) was cloned, overexpressed and ... ...

Abstract	Arginase catalyzes the first committed step in the biosynthesis of polyamines that enable cell growth and hence potential drug target for the treatment of leishmaniasis. The arginase from Leishmania donovani (LdARG) was cloned, overexpressed and characterized. Analysis of the deduced amino acid sequence of LdARG with homologous enzyme from other trypanosomatids arginases identified a non-conserved 12 residues long segment VWGLIERTFLSA from position 161-172. This counter segment in L. mexicana arginase exhibits a different conformation compared with human arginase I. The pH and temperature optima of LdARG were 9.0 and 37 °C, respectively. Biochemical studies revealed that the K
MeSH term(s)	Amino Acid Sequence ; Arginase/genetics ; Arginase/metabolism ; Binding Sites ; Biocatalysis ; Enzyme Inhibitors/metabolism ; Glutamic Acid/chemistry ; Glutamine/chemistry ; Kinetics ; Leishmania donovani/metabolism ; Molecular Dynamics Simulation ; Mutagenesis, Site-Directed ; Mutation ; Polyamines/chemistry ; Protein Binding ; Protein Conformation ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Thermodynamics
Chemical Substances	Enzyme Inhibitors ; Polyamines ; Protozoan Proteins ; Recombinant Proteins ; Glutamine (0RH81L854J) ; Glutamic Acid (3KX376GY7L) ; Arginase (EC 3.5.3.1)
Language	English
Publishing date	2019-03-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1055455-5
ISSN	1096-0279 ; 1046-5928
ISSN (online)	1096-0279
ISSN	1046-5928
DOI	10.1016/j.pep.2019.02.018
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Zs.A 3084: Show issues

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Article ; Online: Leishmanial CpG DNA nanovesicles: A propitious prophylactic approach against visceral leishmaniasis.

Tiwari, Rohit Kumar / Chandrakar, Pragya / Gupta, Chhedi Lal / Sayyed, Uzma / Shekh, Rafia / Bajpai, Preeti

International immunopharmacology

2020 Volume 90, Page(s) 107181

Abstract: Unmethylated CpG motifs with phosphothioate backbone trigger TLR9 to elicit innate immune response characterized by the production of Th1 cytokines. The use of CpG DNA as an adjuvant has established its role in potentiating the humoral and cell mediated ... ...

Abstract	Unmethylated CpG motifs with phosphothioate backbone trigger TLR9 to elicit innate immune response characterized by the production of Th1 cytokines. The use of CpG DNA as an adjuvant has established its role in potentiating the humoral and cell mediated vaccine specific immune response. However, none of the synthetic oligodeoxynucleotides (ODNs) know and used till date are associated with the parasite itself. Our group identified a novel CG rich sequence of 14 base pairs from Leishmania donovani genome (Ld CpG ODN) and established it as a TLR9 agonist. The present study was designed to ascertain the adjuvanticity of Ld CpG ODN with soluble leishmanial antigen in experimental model of L. donovani. During the study Schizophyllan (SPG), a fungal polymer was used for encapsulating Ld CpG ODN for efficient endosomal delivery. The synthesized nanovehicles were of nearly 100 nm and localized within endosomes as confirmed by confocal microscopy. Immunization studies displayed the superior ability of synthesized nanovehicles co-administered with parasite antigen in augmenting innate immune response in comparison to ODN, nanoparticles or soluble antigen alone. The response included generation of ROS, NO and iNOS expression followed by proinflammatory cytokine milieu with reduced parasitic load within liver, spleen and bone marrow. These immune-tailored particles in combination with parasitic antigens elicited significant generation of cell mediated response owing to the presence of high levels of CD8
MeSH term(s)	Adjuvants, Immunologic/administration & dosage ; Adjuvants, Immunologic/chemistry ; Animals ; Antigens, Protozoan/administration & dosage ; Antigens, Protozoan/chemistry ; Disease Models, Animal ; Drug Compounding ; Host-Pathogen Interactions ; Immunity, Cellular/drug effects ; Immunity, Humoral/drug effects ; Immunogenicity, Vaccine ; Leishmania donovani/immunology ; Leishmania donovani/pathogenicity ; Leishmaniasis, Visceral/immunology ; Leishmaniasis, Visceral/parasitology ; Leishmaniasis, Visceral/prevention & control ; Male ; Mesocricetus ; Nanoparticles ; Oligodeoxyribonucleotides/administration & dosage ; Oligodeoxyribonucleotides/chemistry ; Protozoan Vaccines/administration & dosage ; Protozoan Vaccines/chemistry ; Sizofiran/administration & dosage ; Sizofiran/chemistry ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; T-Lymphocytes/parasitology ; Toll-Like Receptor 9/agonists ; Toll-Like Receptor 9/metabolism ; Vaccination
Chemical Substances	Adjuvants, Immunologic ; Antigens, Protozoan ; CPG-oligonucleotide ; Oligodeoxyribonucleotides ; Protozoan Vaccines ; Toll-Like Receptor 9 ; Sizofiran (7F763NNC9X)
Language	English
Publishing date	2020-11-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2020.107181
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Zs.A 5408: Show issues

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Article ; Online: Repolarization of glioblastoma macrophage cells using non-agonistic Dectin-1 ligand encapsulating TLR-9 agonist: plausible role in regenerative medicine against brain tumor.

Tiwari, Rohit Kumar / Singh, Sarika / Gupta, Chhedi Lal / Pandey, Pratibha / Singh, Vipendra Kumar / Sayyed, Uzma / Shekh, Rafia / Bajpai, Preeti

The International journal of neuroscience

2020 Volume 131, Issue 6, Page(s) 591–598

Abstract: Aim of the study: Glioblastoma multiforme (GBM) is the most severe forms of brain cancer, eventually becoming the leading cause of brain cancer-related death worldwide. Owing to the bleak surgical interventions and resistance to the different treatment ... ...

Abstract	Aim of the study: Glioblastoma multiforme (GBM) is the most severe forms of brain cancer, eventually becoming the leading cause of brain cancer-related death worldwide. Owing to the bleak surgical interventions and resistance to the different treatment regime, GBM is a parlous disease demanding newer therapeutical perspective for its treatment. Toll-like receptors (TLRs) are well-known members of pathogen recognition receptors (PRRs) and have been extensively explored for their therapeutic and prophylactic potential in an array of disease including cancer. Recent trends in drug delivery research has shown shift towards delivering short DNA sequences (CpG DNA) to endosomal TLR9 within immune cells (macrophages, dendritic cells, etc.) for the activation of desired inflammatory response using non-agonistic β-glucan particles; a well-known ligand for Dectin-1 receptors. Our study is therefore focused to explore the role of nano-encapsulated CpG ODN as critical players in polarizing M2 scavenging to much desired pro-inflammatory type. Materials and methods: The nanoparticles entrapping CpG ODN 1826 were prepared by using a fungal polymer Schizophyllan (SPG). The constructed nanoparticles were characterized and assessed for their efficacy on rat glioblastoma cells (C6). Results: The constructed Schizophyllan (SPG) nanoparticles entrapping CpG ODN 1826 (95.3%) were of 25.49 nm in diameter and thus capable of crossing blood-brain barrier. The rat glioblastoma (C6) cells evaluated for intracellular oxidative burst and cytokine levels pre- and post-incubation with nanoparticles exhibited marked elevation in the expression of intracellular ROS and IFN-γ as well as IL-1β post treatment. Conclusion: The findings indicate towards potentiality of repolarizing the M2 macrophages to much desired M1 phase by inducing higgh levels of oxidative burst and inflammatory cytokines. Consequently, the apoptosis was induced in glioblastoma cells establishing the suitablity of CpG ODN carrying nanoformulations as emerging therapeutic intervention for GBM.
MeSH term(s)	Adjuvants, Immunologic/administration & dosage ; Animals ; Brain Neoplasms/drug therapy ; Cell Line, Tumor ; Cytokines/drug effects ; Cytokines/metabolism ; Glioblastoma/drug therapy ; Interferon-gamma/drug effects ; Interferon-gamma/metabolism ; Interleukin-1beta/drug effects ; Interleukin-1beta/metabolism ; Lectins, C-Type ; Macrophages/drug effects ; Nanoparticles ; Oligodeoxyribonucleotides ; Rats ; Reactive Oxygen Species/metabolism ; Sizofiran/administration & dosage ; Toll-Like Receptor 9/agonists
Chemical Substances	Adjuvants, Immunologic ; CpG ODN 1826 ; Cytokines ; IL1B protein, rat ; Interleukin-1beta ; Lectins, C-Type ; Oligodeoxyribonucleotides ; Reactive Oxygen Species ; Toll-Like Receptor 9 ; dectin 1 ; Sizofiran (7F763NNC9X) ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2020-04-19
Publishing country	England
Document type	Journal Article
ZDB-ID	3061-2
ISSN	1563-5279 ; 1543-5245 ; 0020-7454
ISSN (online)	1563-5279 ; 1543-5245
ISSN	0020-7454
DOI	10.1080/00207454.2020.1750393
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Zs.A 657: Show issues

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Article: In silico protein modeling: possibilities and limitations.

Gupta, Chhedi Lal / Akhtar, Salman / Bajpai, Preeti

EXCLI journal

2014 Volume 13, Page(s) 513–515

Language	English
Publishing date	2014-05-09
Publishing country	Germany
Document type	Journal Article
ISSN	1611-2156
ISSN	1611-2156
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Article: Spatial and temporal dynamics of microbiomes and resistomes in broiler litter stockpiles.

Gupta, Chhedi Lal / Avidov, Ran / Kattusamy, Karuppasamy / Saadi, Ibrahim / Varma, Vempalli Sudharsan / Blum, Shlomo E / Zhu, Yong-Guan / Zhou, Xin-Yuan / Su, Jian-Qiang / Laor, Yael / Cytryn, Eddie

Computational and structural biotechnology journal

2021 Volume 19, Page(s) 6201–6211

Abstract: Farmers apply broiler chicken litter to soils to enrich organic matter and provide crops with nutrients, following varying periods of stockpiling. However, litter frequently harbors fecal-derived microbial pathogens and associated antibiotic resistance ... ...

Abstract	Farmers apply broiler chicken litter to soils to enrich organic matter and provide crops with nutrients, following varying periods of stockpiling. However, litter frequently harbors fecal-derived microbial pathogens and associated antibiotic resistance genes (ARGs), and may be a source of microbial contamination of produce. We coupled a cutting-edge Loop Genomics long-read 16S rRNA amplicon-sequencing platform with high-throughput qPCR that targeted a suite of ARGs, to assess temporal (five time points over a 60-day period) and spatial (top, middle and bottom layers) microbiome and resistome dynamics in a broiler litter stockpile. We focused on potentially pathogenic species from the
Language	English
Publishing date	2021-11-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2694435-2
ISSN	2001-0370
ISSN	2001-0370
DOI	10.1016/j.csbj.2021.11.020
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