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Article: Position and sequence requirements for poly(A) length regulation by the poly(A) limiting element.

Gupta, J D / Gu, H / Schoenberg, D R

2001 Volume 7, Issue 7, Page(s) 1034–1042

Abstract: The poly(A)-limiting element (PLE) is a cis-acting sequence that acts to limit poly(A) tail length on pre-mRNA to <20 nt. Functional PLEs are present in a number of genes, underscoring the generality of this control mechanism. The current study sought to ...

Abstract	The poly(A)-limiting element (PLE) is a cis-acting sequence that acts to limit poly(A) tail length on pre-mRNA to <20 nt. Functional PLEs are present in a number of genes, underscoring the generality of this control mechanism. The current study sought to define further the position requirements for poly(A) length regulation and the core sequence that comprises a PLE. Increasing the spacing between the PLE and the upstream 3' splice site or between the PLE and the downstream AAUAAA had no effect on poly(A) length control. However, moving the PLE from the terminal exon to either an upstream exon or intron eliminated poly(A) length control. Poly(A) length control was further evaluated using a battery of constructs in which the PLE was maintained in the terminal exon, but where upstream introns were either deleted, modified, or replaced with a polypyrimidine tract. Poly(A) length control was retained in all cases, indicating that the key feature is the presence of the PLE in the terminal exon. A battery of mutations demonstrated the importance of the 5' pyrimidine-rich portion of the element. Finally, UV crosslinking experiments identified an approximately 62-kDa protein in Hela nuclear extract that binds to a wild-type 23-nt PLE RNA oligonucleotides but not to a mutated nonfunctional form of the element.
MeSH term(s)	Base Sequence ; Exons ; HeLa Cells ; Humans ; Introns ; Molecular Sequence Data ; Nuclear Proteins/metabolism ; Poly A/chemistry ; Poly A/genetics ; Poly A/metabolism ; RNA Processing, Post-Transcriptional
Chemical Substances	Nuclear Proteins ; Poly A (24937-83-5)
Language	English
Publishing date	2001-07-09
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	1241540-6
ISSN	1355-8382
ISSN	1355-8382
DOI	10.1017/s1355838201010329
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Article: Polynucleotide phosphorylase is a component of a novel plant poly(A) polymerase.

Li, Q S / Gupta, J D / Hunt, A G

The Journal of biological chemistry

1998 Volume 273, Issue 28, Page(s) 17539–17543

Abstract: We have isolated cDNA clones encoding a novel RNA-binding protein that is a component of a multisubunit poly(A) polymerase from pea seedlings. The encoded protein bears a significant resemblance to polynucleotide phosphorylases (PNPases) from bacteria ... ...

Abstract	We have isolated cDNA clones encoding a novel RNA-binding protein that is a component of a multisubunit poly(A) polymerase from pea seedlings. The encoded protein bears a significant resemblance to polynucleotide phosphorylases (PNPases) from bacteria and chloroplasts. More significantly, this RNA-binding protein is able to degrade RNAs with the resultant production of nucleotide diphosphates, and it can add extended polyadenylate tracts to RNAs using ADP as a donor for adenylate moieties. These activities are characteristic of PNPase. Antibodies raised against the cloned protein simultaneously immunoprecipitate both poly(A) polymerase and PNPase activity. We conclude from these studies that PNPase is the RNA-binding cofactor for this poly(A) polymerase and is an integral player in the reaction catalyzed by this enzyme. The identification of this RNA-binding protein as PNPase, which is a chloroplast-localized enzyme known to be involved in mRNA 3'-end determination and turnover (Hayes, R., Kudla, J., Schuster, G., Gabay, L., Maliga, P., and Gruissem, W. (1996) EMBO J. 15, 1132-1141), raises interesting questions regarding the subcellular location of the poly(A) polymerase under study. We have reexamined this issue, and we find that this enzyme can be detected in chloroplast extracts. The involvement of PNPase in polyadenylation in vitro provides a biochemical rationale for the link between chloroplast RNA polyadenylation and RNA turnover which has been noted by others (Lisitsky, I., Klaff, P., and Schuster, G. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 13398-13403).
MeSH term(s)	Amino Acid Sequence ; Base Sequence ; Chloroplasts/enzymology ; DNA Primers ; Molecular Sequence Data ; Pisum sativum/enzymology ; Polynucleotide Adenylyltransferase/metabolism ; Polyribonucleotide Nucleotidyltransferase/chemistry ; Polyribonucleotide Nucleotidyltransferase/metabolism ; Sequence Homology, Amino Acid
Chemical Substances	DNA Primers ; Polynucleotide Adenylyltransferase (EC 2.7.7.19) ; Polyribonucleotide Nucleotidyltransferase (EC 2.7.7.8)
Language	English
Publishing date	1998-07-10
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.273.28.17539
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Article: Letter: Rubella-antibody titres in rheumatoid arthritis.

Gupta, J D

Lancet (London, England)

1974 Volume 1, Issue 7865, Page(s) 1062–1063

MeSH term(s)	Adult ; Antibodies, Viral/isolation & purification ; Arthritis, Rheumatoid/etiology ; Arthritis, Rheumatoid/immunology ; Cross Reactions ; Humans ; Middle Aged ; Rubella virus/immunology ; Virus Diseases/complications
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	1974-05-25
Publishing country	England
Document type	Journal Article
ZDB-ID	3306-6
ISSN	1474-547X ; 0140-6736 ; 0023-7507
ISSN (online)	1474-547X
ISSN	0140-6736 ; 0023-7507
DOI	10.1016/s0140-6736(74)90476-0
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Article: Rubella-specific IgM antibody as an aid to diagnosis.

Gupta, J D

Lancet (London, England)

1973 Volume 2, Issue 7825, Page(s) 379

MeSH term(s)	Antibodies, Viral/analysis ; Antibody Specificity ; Humans ; Immunoglobulin M/analysis ; Methods ; Rubella/diagnosis ; Rubella virus/immunology ; Time Factors
Chemical Substances	Antibodies, Viral ; Immunoglobulin M
Language	English
Publishing date	1973-08-18
Publishing country	England
Document type	Journal Article
ZDB-ID	3306-6
ISSN	1474-547X ; 0140-6736 ; 0023-7507
ISSN (online)	1474-547X
ISSN	0140-6736 ; 0023-7507
DOI	10.1016/s0140-6736(73)93224-8
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Article: Studies on some constituents of plasma in asthma.

Gupta, J D / Prakash, D

The Indian journal of chest diseases & allied sciences

1988 Volume 30, Issue 1, Page(s) 11–14

MeSH term(s)	Adolescent ; Adult ; Antigen-Antibody Complex/analysis ; Asthma/blood ; Blood Proteins/analysis ; Female ; Humans ; Male ; Middle Aged
Chemical Substances	Antigen-Antibody Complex ; Blood Proteins
Language	English
Publishing date	1988-01
Publishing country	India
Document type	Journal Article
ZDB-ID	416066-6
ISSN	0377-9343 ; 0019-5111
ISSN	0377-9343 ; 0019-5111
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Article: A plant poly(A) polymerase requires a novel RNA-binding protein for activity.

Li, Q S / Gupta, J D / Hunt, A G

The Journal of biological chemistry

1996 Volume 271, Issue 33, Page(s) 19831–19835

Abstract: We have purified a novel factor (PAP-III) that is a component of a multisubunit poly(A) polymerase from pea seedlings. This factor consists of one or more polypeptides with molecular masses of about 105 kDa and of a population of associated RNAs that can ...

Abstract	We have purified a novel factor (PAP-III) that is a component of a multisubunit poly(A) polymerase from pea seedlings. This factor consists of one or more polypeptides with molecular masses of about 105 kDa and of a population of associated RNAs that can serve as substrates for polyadenylation. When these RNAs are separated from the 105-kDa polypeptides, polyadenylation becomes dependent upon exogenously added RNA. This RNA-dependent activity does not require the presence of a polyadenylation signal in the substrate, indicating that the activity under study is a nonspecific polyadenylation activity. One or more of the 105-kDa polypeptides could be cross-linked to the products of polyadenylation labeled with [alpha-32P]ATP and to exogenously added labeled RNAs. Cross-linking of the 105-kDa polypeptides to the products of polyadenylation was not affected by the presence of exogenously added competitors, whereas cross-linking to exogenous RNAs was diminished by excesses of RNA homopolymers. Exogenous RNAs could be polyadenylated by the combination of PAP-I + PAP-III, and this activity was diminished if the binding of the exogenous RNAs to PAP-III was prevented. We conclude from these studies that PAP-III is an RNA binding protein, that polyadenylation by the poly(A) polymerase occurs while the substrate RNAs are associated with this protein, and that the pea poly(A) polymerase can only polyadenylate those RNAs that are associated with PAP-III.
MeSH term(s)	Cross-Linking Reagents ; Macromolecular Substances ; Molecular Weight ; Pisum sativum ; Plant Proteins/chemistry ; Plant Proteins/isolation & purification ; Plant Proteins/metabolism ; Polynucleotide Adenylyltransferase/metabolism ; RNA/metabolism ; RNA, Plant/metabolism ; RNA-Binding Proteins/chemistry ; RNA-Binding Proteins/isolation & purification ; RNA-Binding Proteins/metabolism ; Regulatory Sequences, Nucleic Acid ; Ribonucleoproteins/metabolism
Chemical Substances	Cross-Linking Reagents ; Macromolecular Substances ; Plant Proteins ; RNA, Plant ; RNA-Binding Proteins ; Ribonucleoproteins ; RNA (63231-63-0) ; Polynucleotide Adenylyltransferase (EC 2.7.7.19)
Language	English
Publishing date	1996-08-16
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.271.33.19831
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Article: Role of plants in allergy

Gupta, J.D

Improvement of forest biomass : symposium proceedings / edited by P.K. Khosla.

1982

Title variant	Role of plants in allergy [Pollen, trees and herbaceous plants]
Keywords	pollen
Language	English
Dates of publication	1982-1980
Size	p. 309-314.
Publisher	Indian Society of Tree Scientists, c1982.
Publishing place	Solan, India
Document type	Article
Database	NAL-Catalogue (AGRICOLA)

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Article: Experimental evaluation of preventive and therapeutic potentials of lysozyme.

Das, S / Banerjee, S / Gupta, J D

Chemotherapy

1992 Volume 38, Issue 5, Page(s) 350–357

Abstract: Therapeutic efficacy and preventive role of egg white lysozyme was evaluated in three types of murine ascitic tumours, namely sarcoma 180, Ehrlich's carcinoma, and Dalton's lymphoma. Lysozyme treatment produced regression of tumour growth and improved ... ...

Abstract	Therapeutic efficacy and preventive role of egg white lysozyme was evaluated in three types of murine ascitic tumours, namely sarcoma 180, Ehrlich's carcinoma, and Dalton's lymphoma. Lysozyme treatment produced regression of tumour growth and improved the life expectancy of the host. Growth of tumour cells treated in vitro with lysozyme prior to transplantation was also affected. In addition, lysozyme was found to have a preventive effect when administered to normal mice. The antitumour activity, therapeutic and preventive, of lysozyme seems to be due to its action on the tumour cell surface as well as on the host-mediated immune response.
MeSH term(s)	Animals ; Carcinoma, Ehrlich Tumor/drug therapy ; Carcinoma, Ehrlich Tumor/prevention & control ; Egg White ; Lymphoma/drug therapy ; Lymphoma/prevention & control ; Mice ; Muramidase/therapeutic use ; Neoplasm Transplantation ; Neoplasms, Experimental/drug therapy ; Neoplasms, Experimental/prevention & control ; Peritoneal Cavity/pathology ; Sarcoma 180/drug therapy ; Sarcoma 180/prevention & control ; Tumor Cells, Cultured
Chemical Substances	Muramidase (EC 3.2.1.17)
Language	English
Publishing date	1992
Publishing country	Switzerland
Document type	Comparative Study ; Journal Article
ZDB-ID	6708-8
ISSN	1421-9794 ; 0009-3157
ISSN (online)	1421-9794
ISSN	0009-3157
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Article ; Conference proceedings: Political regimes and rural development

Gupta, J.D

Kansas. Agricultural Experiment Station. Monograph - Kansas Agricultural Experiment Station. July 1978. (1)

1978

Title variant	Political regimes [in developing countries] and rural development
Event/congress	Political Aspects of World Food Problems (1977, KansasStateUniversity)
Keywords	developing countries
Language	English
Dates of publication	1978-07
Size	p. 58-71.
Document type	Article ; Conference proceedings
Database	NAL-Catalogue (AGRICOLA)

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Article: Gastro-duodenal mucosal changes associated with low-dose aspirin therapy: a prospective, endoscopic study.

Chowdhury, A / Ganguly, G / Chowdhury, D / Santra, A / Gupta, J D / Roy, T

Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology

2001 Volume 20, Issue 6, Page(s) 227–229

Abstract: Objectives: The association of low-dose aspirin use and gastro-intestinal bleeding is well described. However, the gastroduodenal mucosal changes associated with low-dose aspirin therapy have not been properly evaluated. We undertook a prospective, ... ...

Abstract	Objectives: The association of low-dose aspirin use and gastro-intestinal bleeding is well described. However, the gastroduodenal mucosal changes associated with low-dose aspirin therapy have not been properly evaluated. We undertook a prospective, endoscopic study to evaluate gastro-duodenal mucosal lesions produced by low-dose aspirin. Methods: Forty-seven patients with non-hemorrhagic cerebral infarct or transient ischemic attacks and normal upper gastrointestinal endoscopy were randomized to receive either enteric-coated (n=25) or plain (n=22) aspirin (150 mg/day). Follow-up endoscopy was done at 2, 4 and 8 weeks; gastro-duodenal mucosal lesions, if present, were scored. Forty-seven patients with hemorrhagic infarct who were not treated with aspirin served as controls. Results: Twenty eight (60%) of 47 patients receiving aspirin had mucosal lesions; stomach alone was the most frequent site (32%), followed by both stomach and duodenum (23%). Frequency of mucosal changes in the stomach at 8 weeks (19%) was significantly lower (p<0.05) than those at 2 weeks (53%) and 4 weeks (55%). Coated (56%) and plain (63.6%) aspirin induced mucosal lesions with similar frequency. Conclusion: Administration of low-dose aspirin, either plain or enteric-coated, induces endoscopic gastro-duodenal mucosal lesions in a large majority of patients. The frequency of damage decreased after 8 weeks of therapy.
MeSH term(s)	Adult ; Aged ; Anti-Inflammatory Agents, Non-Steroidal/administration & dosage ; Anti-Inflammatory Agents, Non-Steroidal/adverse effects ; Aspirin/administration & dosage ; Aspirin/adverse effects ; Cerebral Infarction/drug therapy ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Endoscopy, Gastrointestinal/methods ; Female ; Follow-Up Studies ; Gastric Mucosa/drug effects ; Gastric Mucosa/pathology ; Humans ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/pathology ; Ischemic Attack, Transient/drug therapy ; Male ; Middle Aged ; Prospective Studies ; Reference Values ; Risk Assessment ; Risk Factors ; Statistics, Nonparametric ; Tablets, Enteric-Coated
Chemical Substances	Anti-Inflammatory Agents, Non-Steroidal ; Tablets, Enteric-Coated ; Aspirin (R16CO5Y76E)
Language	English
Publishing date	2001-08-08
Publishing country	India
Document type	Clinical Trial ; Comparative Study ; Journal Article ; Randomized Controlled Trial
ZDB-ID	632595-6
ISSN	0254-8860
ISSN	0254-8860
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