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  1. Article ; Online: Silicon in ultrafresh groundwater

    Guseva Natalia V. / Kopylova Yuliya G. / Vorobeva Daria A / Khvashchevskaya Albina A. / Evtyugina Zinaida A.

    E3S Web of Conferences, Vol 98, p

    a case study of the Imandra Lake catchment, The Kola Peninsula

    2019  Volume 01018

    Abstract: The ultrafresh groundwater (with TDS values less than 200 mg/L) of the Imandra Lake catchment, Kola Peninsula, is from an intensive water exchange zone, where the water has a short period of contact with the rock. Therefore, the considered water is at ... ...

    Abstract The ultrafresh groundwater (with TDS values less than 200 mg/L) of the Imandra Lake catchment, Kola Peninsula, is from an intensive water exchange zone, where the water has a short period of contact with the rock. Therefore, the considered water is at the initial stages of the water–rock interaction. The water is saturated with respect to oxides and hydroxides of aluminium and iron. In the groundwater of the Imandra Lake catchment area, the silicon concentrations significantly exceed the concentrations of magnesium and especially potassium. Nevertheless, water is undersaturated with respect to with respect to silicon oxides. The shown enrichment of water with cations is explained by time of water-rock interaction.
    Keywords Environmental sciences ; GE1-350
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Primary cultures of female swine genital epithelial cells in vitro: a new approach for the study of hormonal modulation of Chlamydia infection.

    Guseva, Natalia V / Knight, Stephen T / Whittimore, Judy D / Wyrick, Priscilla B

    Infection and immunity

    2003  Volume 71, Issue 8, Page(s) 4700–4710

    Abstract: Previous studies have demonstrated that female reproductive hormones influence chlamydial infection both in vivo and in vitro. Due to the reduced availability of human genital tissues for research purposes, an alternative hormone-responsive model system ... ...

    Abstract Previous studies have demonstrated that female reproductive hormones influence chlamydial infection both in vivo and in vitro. Due to the reduced availability of human genital tissues for research purposes, an alternative hormone-responsive model system was sought to study chlamydial pathogenesis. Mature female swine eliminated from breeding programs were selected as the animals of choice because of the similarity of a sexually transmitted disease syndrome and sequelae in swine to a disease syndrome and sequelae found in humans, because of the near identity of a natural infectious chlamydial isolate from swine to Chlamydia trachomatis serovar D from humans, and because a pig's epithelial cell physiology and the mean length of its estrous cycle are similar to those in humans. Epithelial cells from the cervix, uterus, and horns of the uterus were isolated, cultivated in vitro in Dulbecco's minimum essential medium-Hanks' F-12 (DMEM-F-12) medium with and without exogenous hormone supplementation, and analyzed for Chlamydia suis S-45 infectivity. The distribution of chlamydial inclusions in swine epithelial cells was uneven and was influenced by the genital tract site and hormone status. This study confirmed that, like primary human endometrial epithelial cells, estrogen-dominant swine epithelial cells are more susceptible to chlamydial infection than are progesterone-dominant cells. Further, the more differentiated luminal epithelial cells were more susceptible to infection than were glandular epithelial cells. Interestingly, chlamydial growth in mature luminal epithelia was morphologically more active than in glandular epithelia, where persistent chlamydial forms predominated. Attempts to reprogram epithelial cell physiology and thereby susceptibility to chlamydial infection by reverse-stage, exogenous hormonal supplementation were unsuccessful. Freshly isolated primary pig epithelial cells frozen at -80 degrees C in DMEM-F-12 medium with 10% dimethyl sulfoxide for several weeks can, after thawing, reform characteristic polarized monolayers in 3 to 5 days. Thus, primary swine genital epithelia cultured ex vivo appear to be an excellent cell model for dissecting the hormonal modulation of several aspects of chlamydial pathogenesis and infection.
    MeSH term(s) Animals ; Bacterial Adhesion/drug effects ; Cells, Cultured ; Cervix Uteri/cytology ; Chlamydia/pathogenicity ; Chlamydia Infections/etiology ; Chlamydia Infections/metabolism ; Chlamydia Infections/pathology ; Epithelial Cells/cytology ; Estradiol/pharmacology ; Female ; Gonadal Steroid Hormones/metabolism ; Gonadal Steroid Hormones/pharmacology ; Progesterone/pharmacology ; Sus scrofa ; Uterus/cytology
    Chemical Substances Gonadal Steroid Hormones ; Progesterone (4G7DS2Q64Y) ; Estradiol (4TI98Z838E)
    Language English
    Publishing date 2003-08
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/IAI.71.8.4700-4710.2003
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  3. Article: Differences in Chlamydia trachomatis Serovar E Growth Rate in Polarized Endometrial and Endocervical Epithelial Cells Grown in Three-Dimensional Culture

    Guseva, Natalia V / Dessus-Babus, Sophie / Moore, Cheryl G / Whittimore, Judy D / Wyrick, Priscilla B

    Infection and immunity. 2007 Feb., v. 75, no. 2

    2007  

    Abstract: In vitro studies of obligate intracellular chlamydia biology and pathogenesis are highly dependent on the use of experimental models and growth conditions that mimic the mucosal architecture and environment these pathogens encounter during natural ... ...

    Abstract In vitro studies of obligate intracellular chlamydia biology and pathogenesis are highly dependent on the use of experimental models and growth conditions that mimic the mucosal architecture and environment these pathogens encounter during natural infections. In this study, the growth of Chlamydia trachomatis genital serovar E was monitored in mouse fibroblast McCoy cells and compared to more relevant host human epithelial endometrium-derived HEC-1B and cervix-derived HeLa cells, seeded and polarized on collagen-coated microcarrier beads, using a three-dimensional culture system. Microscopy analysis of these cell lines prior to infection revealed morphological differences reminiscent of their in vivo architecture. Upon infection, early chlamydial inclusion distribution was uniform in McCoy cells but patchy in both epithelial cell lines. Although no difference in chlamydial attachment to or entry into the two genital epithelial cell lines was noted, active bacterial genome replication and transcription, as well as initial transformation of elementary bodies to reticulate bodies, were detected earlier in HEC-1B than in HeLa cells, suggesting a faster growth, which led to higher progeny counts and titers in HEC-1B cells upon completion of the developmental cycle. Chlamydial development in the less relevant McCoy cells was very similar to that in HeLa cells, although higher progeny counts were obtained. In conclusion, this three-dimensional bead culture system represents an improved model for harvesting large quantities of infectious chlamydia progeny from their more natural polarized epithelial host cells.
    Keywords Chlamydia trachomatis ; endometrium ; epithelial cells ; fibroblasts ; genome ; growth models ; harvesting ; humans ; in vitro studies ; mice ; microscopy ; pathogenesis ; pathogens ; progeny ; serotypes ; transcription (genetics)
    Language English
    Size p. 553-564.
    Publishing place American Society for Microbiology
    Document type Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

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  4. Article: Differences in Chlamydia trachomatis serovar E growth rate in polarized endometrial and endocervical epithelial cells grown in three-dimensional culture.

    Guseva, Natalia V / Dessus-Babus, Sophie / Moore, Cheryl G / Whittimore, Judy D / Wyrick, Priscilla B

    Infection and immunity

    2006  Volume 75, Issue 2, Page(s) 553–564

    Abstract: In vitro studies of obligate intracellular chlamydia biology and pathogenesis are highly dependent on the use of experimental models and growth conditions that mimic the mucosal architecture and environment these pathogens encounter during natural ... ...

    Abstract In vitro studies of obligate intracellular chlamydia biology and pathogenesis are highly dependent on the use of experimental models and growth conditions that mimic the mucosal architecture and environment these pathogens encounter during natural infections. In this study, the growth of Chlamydia trachomatis genital serovar E was monitored in mouse fibroblast McCoy cells and compared to more relevant host human epithelial endometrium-derived HEC-1B and cervix-derived HeLa cells, seeded and polarized on collagen-coated microcarrier beads, using a three-dimensional culture system. Microscopy analysis of these cell lines prior to infection revealed morphological differences reminiscent of their in vivo architecture. Upon infection, early chlamydial inclusion distribution was uniform in McCoy cells but patchy in both epithelial cell lines. Although no difference in chlamydial attachment to or entry into the two genital epithelial cell lines was noted, active bacterial genome replication and transcription, as well as initial transformation of elementary bodies to reticulate bodies, were detected earlier in HEC-1B than in HeLa cells, suggesting a faster growth, which led to higher progeny counts and titers in HEC-1B cells upon completion of the developmental cycle. Chlamydial development in the less relevant McCoy cells was very similar to that in HeLa cells, although higher progeny counts were obtained. In conclusion, this three-dimensional bead culture system represents an improved model for harvesting large quantities of infectious chlamydia progeny from their more natural polarized epithelial host cells.
    MeSH term(s) Animals ; Cell Culture Techniques/methods ; Cell Line ; Cell Polarity ; Chlamydia trachomatis/growth & development ; Cytoplasm/microbiology ; DNA Replication ; DNA, Bacterial/analysis ; Endometrium/cytology ; Epithelial Cells/cytology ; Epithelial Cells/microbiology ; Female ; Fibroblasts/microbiology ; HeLa Cells ; Humans ; Inclusion Bodies ; Mice ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Microspheres ; RNA, Bacterial/analysis ; Transcription, Genetic
    Chemical Substances DNA, Bacterial ; RNA, Bacterial
    Language English
    Publishing date 2006-11-06
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/IAI.01517-06
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  5. Article: Characterization of estrogen-responsive epithelial cell lines and their infectivity by genital Chlamydia trachomatis.

    Guseva, Natalia V / Dessus-Babus, Sophie C / Whittimore, Judy D / Moore, Cheryl G / Wyrick, Priscilla B

    Microbes and infection

    2005  Volume 7, Issue 15, Page(s) 1469–1481

    Abstract: Chlamydial attachment and infectivity in vitro and ascending disease and sequelae in vivo have been reported to be enhanced/modulated by estrogen. Endometrial carcinoma cell lines Ishikawa and HEC-1B and the breast cancer lines MCF-7 and HCC-1806 were ... ...

    Abstract Chlamydial attachment and infectivity in vitro and ascending disease and sequelae in vivo have been reported to be enhanced/modulated by estrogen. Endometrial carcinoma cell lines Ishikawa and HEC-1B and the breast cancer lines MCF-7 and HCC-1806 were examined for Chlamydia trachomatis E infectivity. Estrogen receptor (ER) presence was confirmed by Western blot and qRT-PCR analyses. FACS analysis was used to determine the percent of plasma membrane-localized ERs (mERs), and their activity was tested by estrogen binding and competitive estrogen antagonists assays. Chlamydiae grew in all cell lines with HEC (90%) >> MCF-7 (57%)>Ishikawa (51%) >> HCC-1806 (20%). The cell line ER isoform composition was re-defined as: ERalpha + ERbeta + for MCF-7, HCC-1806 and Ishikawa; and ERbeta only for HEC-1B. HeLa cells were also tested and found to express ERbeta, but not ERalpha. A small percentage of both ERs were surface-exposed and functionally active. The endometrium-predominant ERbeta isoform was found in all cell lines, including those most representative of the common sites of C. trachomatis infection. Thus, the role of chlamydial attachment/infectivity will now be analyzed in ERbeta+and-isogenic HEC-1B cells.
    MeSH term(s) Blotting, Western ; Cell Line ; Cell Membrane/chemistry ; Chlamydia trachomatis/growth & development ; Cytoplasm/microbiology ; Cytoplasm/ultrastructure ; Epithelial Cells/microbiology ; Epithelial Cells/ultrastructure ; Estrogens/analysis ; Estrogens/physiology ; Flow Cytometry ; Gene Expression ; Humans ; Inclusion Bodies/microbiology ; Inclusion Bodies/ultrastructure ; Microscopy, Confocal ; RNA, Messenger/analysis ; Receptors, Estrogen/analysis ; Receptors, Estrogen/genetics ; Reverse Transcriptase Polymerase Chain Reaction
    Chemical Substances Estrogens ; RNA, Messenger ; Receptors, Estrogen
    Language English
    Publishing date 2005-12
    Publishing country France
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1465093-9
    ISSN 1769-714X ; 1286-4579
    ISSN (online) 1769-714X
    ISSN 1286-4579
    DOI 10.1016/j.micinf.2005.05.004
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