Article ; Online: Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation.
2021 Volume 28, Page(s) 65
Abstract: Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their ... ...
Title translation | Évaluation de l’immunoprotection du domaine N-terminal recombinant des récepteurs Cys-loop contre l’infestation par les tiques Rhipicephalus (Boophilus) microplus. | |||||
---|---|---|---|---|---|---|
Abstract | Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their uncontrolled use has increased resistant tick populations, as well as food and environmental contamination. Alternative immunological tick control has shown to be partially effective. Therefore, there is a need to characterize novel antigens in order to improve immunological protection. The aim of this work was to evaluate Cys-loop receptors as vaccine candidates. N-terminal domains of a glutamate receptor and of a glycine-like receptor were recombinantly produced in Escherichia coli. Groups of BALB/c mice were independently immunized with four doses of each recombinant protein emulsified with Freund's adjuvant. Both vaccine candidates were immunogenic in mice as demonstrated by western blot analysis. Next, recombinant proteins were independently formulated with the adjuvant Montanide ISA 50 V2 and evaluated in cattle infested with Rhipicephalus microplus tick larvae. Groups of three European crossbred calves were immunized with three doses of each adjuvanted protein. ELISA test was used to evaluate the IgG immune response elicited against the recombinant proteins. Results showed that vaccine candidates generated a moderate humoral response on vaccinated cattle. Vaccination significantly affected the number of engorged adult female ticks, having no significant effects on tick weight, egg weight and egg fertility values. Vaccine efficacies of 33% and 25% were calculated for the glutamate receptor and the glycine-like receptor, respectively. |
|||||
MeSH term(s) | Animals ; Cattle ; Cattle Diseases/prevention & control ; Cysteine Loop Ligand-Gated Ion Channel Receptors ; Female ; Mice ; Mice, Inbred BALB C ; Rhipicephalus ; Tick Infestations/prevention & control ; Tick Infestations/veterinary | |||||
Chemical Substances | Cysteine Loop Ligand-Gated Ion Channel Receptors | |||||
Language | English | |||||
Publishing date | 2021-09-14 | |||||
Publishing country | France | |||||
Document type | Journal Article | |||||
ZDB-ID | 1187629-3 | |||||
ISSN | 1776-1042 ; 1252-607X | |||||
ISSN (online) | 1776-1042 | |||||
ISSN | 1252-607X | |||||
DOI | 10.1051/parasite/2021064 | |||||
Shelf mark |
|
|||||
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
Zs.A 3965: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.