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  1. Book ; Audio / Video ; Online: Antiviral effect of two polyphenol-enriched plant extracts against herpes simplex virus type

    Hafezi, W.

    2009  

    Language English
    Publishing date 2009-03-19
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Book ; Audio / Video ; Online: Antiviral effect of two polyphenol-enriched plant extracts against herpes simplex virus type

    Hafezi, W.

    2009  

    Keywords Text
    Language English
    Publishing date 2009-03-19
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Umgang mit COVID-19 in der Notaufnahme : Erfahrungsbericht der interdisziplinären Notaufnahme des Universitätsklinikums Münster.

    Wennmann, D O / Dlugos, C P / Hofschröer, A / Hennies, M / Kühn, J / Hafezi, W / Kampmeier, S / Mellmann, A / Triphaus, S / Sackarnd, J / Tepasse, P / Keller, M / Van Aken, H / Pavenstädt, H / Kümpers, P

    Medizinische Klinik, Intensivmedizin und Notfallmedizin

    2020  Volume 115, Issue 5, Page(s) 380–387

    Abstract: With the COVID-19 pandemic, emergency rooms are faced with major challenges because they act as the interface between outpatient and inpatient care. The dynamics of the pandemic forced emergency care at the University Hospital Münster to extensively ... ...

    Title translation Handling of COVID-19 in the emergency department : Field report of the emergency ward of the University Hospital Münster.
    Abstract With the COVID-19 pandemic, emergency rooms are faced with major challenges because they act as the interface between outpatient and inpatient care. The dynamics of the pandemic forced emergency care at the University Hospital Münster to extensively adjust their processes, which had to be carried out in the shortest time possible. This included the establishment of an outpatient coronavirus test center and a medical student-operated telephone hotline. Inside the hospital, new isolation capacities in the emergency room and a dedicated COVID-19 ward were set up. The patient flow was reorganized using flow diagrams for both the outpatient and inpatient areas. The general and special emergency management was optimized for the efficient treatment of COVID-19-positive patients and the staff were trained in the use of protective equipment. This report of our experience is intended to support other emergency departments in their preparation for the COVID-19 pandemic.
    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections/epidemiology ; Emergency Medical Services ; Emergency Service, Hospital ; Humans ; Pandemics ; Patient Isolation ; Pneumonia, Viral/epidemiology ; SARS-CoV-2 ; Triage
    Keywords covid19
    Language German
    Publishing date 2020-04-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2636018-4
    ISSN 2193-6226 ; 1435-1420 ; 0723-5003 ; 2193-6218 ; 0175-3851
    ISSN (online) 2193-6226 ; 1435-1420
    ISSN 0723-5003 ; 2193-6218 ; 0175-3851
    DOI 10.1007/s00063-020-00693-0
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  4. Article ; Online: In vivo visualization of encephalitic lesions in herpes simplex virus type 1 (HSV-1) infected mice by magnetic resonance imaging (MRI).

    Hafezi, Wali / Hoerr, Verena

    Methods in molecular biology (Clifton, N.J.)

    2013  Volume 1064, Page(s) 253–265

    Abstract: Herpes simplex encephalitis (HSE) is one of the most severe viral infections affecting the temporal lobes of the brain. Despite the improvements in diagnosis and antiviral drug treatment, one third of all patients fail to respond to therapy or ... ...

    Abstract Herpes simplex encephalitis (HSE) is one of the most severe viral infections affecting the temporal lobes of the brain. Despite the improvements in diagnosis and antiviral drug treatment, one third of all patients fail to respond to therapy or subsequently suffer neurological relapse and develop long term neurological damage. Magnetic resonance imaging (MRI) is among the appropriate noninvasive tools for early diagnosis of viral central nervous system (CNS) infections. In this chapter we introduce a mouse model for HSE and describe a MRI protocol to characterize the pathogenesis of HSE over time.
    MeSH term(s) Animals ; Brain/pathology ; Brain/virology ; Cell Line ; Encephalitis, Herpes Simplex/diagnosis ; Encephalitis, Herpes Simplex/virology ; Female ; Herpesvirus 1, Human/physiology ; Humans ; Magnetic Resonance Imaging ; Mice
    Language English
    Publishing date 2013
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-62703-601-6_18
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Conference proceedings: ID NOW Point-of-Care Testgeräte sind zuverlässig und kosteneffektiv beim initialen COVID-19 Screening in einer unfallchirurgischen Notaufnahme

    Stolberg-Stolberg, Josef / Jacob, Elena / Hennies, Marc / Hafezi, Wali / Kühn, Joachim / Katthagen, Jan Christoph / Raschke, Michael J.

    2022  , Page(s) AB26–1182

    Event/congress Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2022); Berlin; ; Berufsverband für Orthopädie und Unfallchirurgie; 2022
    Keywords Medizin, Gesundheit ; Point-of-Care Testgerät ; COVID-19 ; Notaufnahme ; Schockraum
    Publishing date 2022-10-25
    Publisher German Medical Science GMS Publishing House; Düsseldorf
    Document type Conference proceedings
    DOI 10.3205/22dkou138
    Database German Medical Science

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  6. Article ; Conference proceedings: Plant derived natural products as novel fusion inhibitors against Herpes simplex virus type 1 (HSV-1)

    Ulrich, D / Brandt, S / Hafezi, W / Kühn, J / Hensel, A

    Planta Medica

    2015  

    Abstract: Herpesvirus infection and spread can be specifically blocked by preventing the fusion between the virion and the host cell membrane. The core fusion machinery of HSV-1 consists of the glycoproteins gD, gH, gL and gB. While gD mediates the interaction ... ...

    Event/congress GA 2015 - Book of Abstracts, Budapest, Hungary, 2015
    Abstract Herpesvirus infection and spread can be specifically blocked by preventing the fusion between the virion and the host cell membrane. The core fusion machinery of HSV-1 consists of the glycoproteins gD, gH, gL and gB. While gD mediates the interaction with various host cell receptors, gB executes the fusion of viral with cellular membrane after activation by a reaction cascade between the glycoproteins. Based on this mechanism a virus-free in vitro screening assay was developed for direct identification of antiviral compounds with fusion-inhibiting capability. Vero cells are transfected with gD, gH, gL and gB (effector-cells) and seeded on untransfected Vero cells (target-cells). The formation of syncytia and thereby the amount of fusion is visualized through mCherry-labelled gB. To quantify fusion activity, effector cells transfer a transactivator into the target cells, which in turn switches on a reporter gene, e.g. luciferase [1]. The use of Tet-On 3G as transactivator reduced cytotoxicity, widened the measureable window and allowed selective induction of reporter gene expression. Docosanol (5 mg/mL), a known entry inhibitor of enveloped viruses, and α-gB-2c, a neutralising, gB-specific monoclonal antibody, served as positive controls. Aescin from Aesculus hippocastanum was identified as a potent fusion inhibitor against HSV-1. Two different batches, characterized in detail by LC-MS, showed IC 50 between 5 and 10 µM, depending on incubation time and serum concentration in the cultivation media. Aescin reduces also HSV-1 plaque formation. Within a broader screening of saponins as fusion inhibitors we identified 2 active oleanan glycosides: hederacoside C (IC 50 about 200 µM) from Hedera helix, and esculentoside A (IC 50 about 150 µM) from Phytolacca esculenta
    References: 1] PE Pertel, A Fridberg, ML Parish, PG Spear. Cell fusion induced by herpes simplex virus glycoproteins gB, gD, and gH-gL requires a gD receptor but not necessarily heparan sulfate. Virology 2001; 279: 313 – 24
    Language English
    Publishing date 2015-11-25
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 123545-x
    ISSN 1439-0221 ; 0032-0943
    ISSN (online) 1439-0221
    ISSN 0032-0943
    DOI 10.1055/s-0035-1565369
    Database Thieme publisher's database

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  7. Article ; Conference proceedings: Antiviral effect of polyphenol-enriched extract of Rumex acetosa L. against influenza A virus

    Derksen, A / Kühn, J / Hafezi, W / Hensel, A

    Zeitschrift für Phytotherapie

    2013  

    Abstract: Although effective antiviral drugs are available for the treatment of influenza A virus (IAV) infection, the need for intensified drug discovery towards antiviral active compounds is obvious. A polyphenol-enriched extract of Rumex acetosa L. ( ... ...

    Event/congress Phytokongress 2013, Leipzig, 2013
    Abstract Although effective antiviral drugs are available for the treatment of influenza A virus (IAV) infection, the need for intensified drug discovery towards antiviral active compounds is obvious. A polyphenol-enriched extract of Rumex acetosa L. (Polygonaceae), standardized on galloylated oligomeric proanthocyanidins, was tested for antiviral activity against IAV in vitro using MTT, plaque reduction and hemagglutination assay. The extract inhibited IAV replication in a dose-dependent manner. IC 50 and CC 50 values, as determined by MTT assay, were 2.2 µg/ml and 79.2 µg/ml, resp., indicating a CC 50 /IC 50 selectivity ratio of 36. This antiviral activity was confirmed by plaque reduction assay. To determine the mechanism of this antiviral effect, the extract was added at different stages during the viral replication cycle. The strongest antiviral effect was observed when the extract was added before adsorption of IAV to the cells, but in high concentrations an effect was also detected when extract was added after adsorption of IAV to and even after penetration of IAV into the cells, indicating the antiviral effect being associated not only with viral adsorption and penetration. Structure-activity relation of flavan-3-ols and oligomeric proanthocyanidins indicated that trihydroxylation of ring B or alternatively galloylation at position O-3 potentiate the antiviral activity. Detailed studies using the model compound procyanidin-B2-digallate indicated this compound as a potent inhibitor of IAV.
    Language English
    Publishing date 2013-03-01
    Publishing place Stuttgart ; New York
    Document type Article ; Conference proceedings
    ZDB-ID 604850-x
    ISSN 1438-9584 ; 0722-348X ; 0720-227X
    ISSN (online) 1438-9584
    ISSN 0722-348X ; 0720-227X
    DOI 10.1055/s-0033-1338220
    Database Thieme publisher's database

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    Zs.B 2525: Show issues Location:
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  8. Article ; Online: Broadly Applicable, Virus-Free Dual Reporter Assay to Identify Compounds Interfering with Membrane Fusion: Performance for HSV-1 and SARS-CoV-2.

    Classen, Nica / Ulrich, Diana / Hofemeier, Arne / Hennies, Marc Tim / Hafezi, Wali / Pettke, Aleksandra / Romberg, Marie-Luise / Lorentzen, Eva U / Hensel, Andreas / Kühn, Joachim E

    Viruses

    2022  Volume 14, Issue 7

    Abstract: Membrane fusion constitutes an essential step in the replication cycle of numerous viral pathogens, hence it represents an important druggable target. In the present study, we established a virus-free, stable reporter fusion inhibition assay (SRFIA) ... ...

    Abstract Membrane fusion constitutes an essential step in the replication cycle of numerous viral pathogens, hence it represents an important druggable target. In the present study, we established a virus-free, stable reporter fusion inhibition assay (SRFIA) specifically designed to identify compounds interfering with virus-induced membrane fusion. The dual reporter assay is based on two stable Vero cell lines harboring the third-generation tetracycline (Tet3G) transactivator and a bicistronic reporter gene cassette under the control of the tetracycline responsive element (TRE3G), respectively. Cell-cell fusion by the transient transfection of viral fusogens in the presence of doxycycline results in the expression of the reporter enzyme secreted alkaline phosphatase (SEAP) and the fluorescent nuclear localization marker EYFPNuc. A constitutively expressed, secreted form of nanoluciferase (secNLuc) functioned as the internal control. The performance of the SRFIA was tested for the quantification of SARS-CoV-2- and HSV-1-induced cell-cell fusion, respectively, showing high sensitivity and specificity, as well as the reliable identification of known fusion inhibitors. Parallel quantification of secNLuc enabled the detection of cytotoxic compounds or insufficient transfection efficacy. In conclusion, the SRFIA reported here is well suited for high-throughput screening for new antiviral agents and essentially will be applicable to all viral fusogens causing cell-cell fusion in Vero cells.
    MeSH term(s) Animals ; Antiviral Agents/pharmacology ; COVID-19 ; Cell Line ; Chlorocebus aethiops ; Genes, Reporter ; Herpesvirus 1, Human/genetics ; Humans ; Membrane Fusion ; SARS-CoV-2/genetics ; Tetracyclines ; Vero Cells
    Chemical Substances Antiviral Agents ; Tetracyclines
    Language English
    Publishing date 2022-06-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14071354
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: COVID-19 rapid molecular point-of-care testing is effective and cost-beneficial for the acute care of trauma patients.

    Stolberg-Stolberg, Josef / Jacob, Elena / Kuehn, Joachim / Hennies, Marc / Hafezi, Wali / Freistuehler, Moritz / Koeppe, Jeanette / Friedrich, Alex W / Katthagen, J Christoph / Raschke, Michael J

    European journal of trauma and emergency surgery : official publication of the European Trauma Society

    2022  Volume 49, Issue 1, Page(s) 487–493

    Abstract: Purpose: To evaluate the accuracy and cost benefit of a rapid molecular point-of-care testing (POCT) device detecting COVID-19 within a traumatological emergency department.: Background: Despite continuous withdrawal of COVID-19 restrictions, ... ...

    Abstract Purpose: To evaluate the accuracy and cost benefit of a rapid molecular point-of-care testing (POCT) device detecting COVID-19 within a traumatological emergency department.
    Background: Despite continuous withdrawal of COVID-19 restrictions, hospitals will remain particularly vulnerable to local outbreaks which is reflected by a higher institution-specific basic reproduction rate. Patients admitted to the emergency department with unknown COVID-19 infection status due to a- or oligosymptomatic COVID-19 infection put other patients and health care workers at risk, while fast diagnosis and treatment is necessary. Delayed testing results in additional costs to the health care system.
    Methods: From the 8th of April 2021 until 31st of December 2021, all patients admitted to the emergency department were tested with routine RT-PCR and rapid molecular POCT device (Abbott ID NOW™ COVID-19). COVID-19-related additional costs for patients admitted via shock room or emergency department were calculated based on internal cost allocations.
    Results: 1133 rapid molecular tests resulted in a sensitivity of 83.3% (95% CI 35.9-99.6%), specificity of 99.8% (95% CI 99.4-100%), a positive predictive value of 71.4% (95% CI 29-96.3%) and a negative predictive value of 99.9% (95% CI 99.5-100%) as compared to RT-PCR. Without rapid COVID-19 testing, each emergency department and shock room admission with subsequent surgery showed additional direct costs of 2631.25€, without surgery of 729.01€.
    Conclusion: Although rapid molecular COVID-19 testing can initially be more expensive than RT-PCR, subsequent cost savings, improved workflows and workforce protection outweigh this effect by far. The data of this study support the use of a rapid molecular POCT device in a traumatological emergency department.
    MeSH term(s) Humans ; COVID-19/diagnosis ; COVID-19 Testing ; Point-of-Care Systems ; Sensitivity and Specificity ; Point-of-Care Testing
    Language English
    Publishing date 2022-09-06
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2275480-5
    ISSN 1863-9941 ; 1863-9933
    ISSN (online) 1863-9941
    ISSN 1863-9933
    DOI 10.1007/s00068-022-02091-x
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  10. Article ; Online: SARS-CoV-2 in the Employees of a Large University Hospital.

    Schwierzeck, Vera / Correa-Martinez, Carlos Luis / Schneider, Kristian Nikolaus / Mellmann, Alexander / Hennies, Marc Tim / Hafezi, Wali / Czeschinski, Peter / Kampmeier, Stefanie

    Deutsches Arzteblatt international

    2020  Volume 117, Issue 19, Page(s) 344–345

    MeSH term(s) Betacoronavirus/isolation & purification ; COVID-19 ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Hospitals, University ; Humans ; Pandemics ; Personnel, Hospital/statistics & numerical data ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/epidemiology ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-06-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2406159-1
    ISSN 1866-0452 ; 1866-0452
    ISSN (online) 1866-0452
    ISSN 1866-0452
    DOI 10.3238/arztebl.2020.0344
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