Article ; Online: An optimized purification protocol for enzymatically synthesized S-adenosyl-L-methionine (SAM) for applications in solution state infrared spectroscopic studies.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
2024 Volume 309, Page(s) 123816
Abstract: S-adenosyl-L-methionine (SAM) is an abundant biomolecule used by methyltransferases to regulate a wide range of essential cellular processes such as gene expression, cell signaling, protein functions, and metabolism. Despite considerable effort, there ... ...
| Abstract | S-adenosyl-L-methionine (SAM) is an abundant biomolecule used by methyltransferases to regulate a wide range of essential cellular processes such as gene expression, cell signaling, protein functions, and metabolism. Despite considerable effort, there remain many specificity challenges associated with designing small molecule inhibitors for methyltransferases, most of which exhibit off-target effects. Interestingly, NMR evidence suggests that SAM undergoes conformeric exchange between several states when free in solution. Infrared spectroscopy can detect different conformers of molecules if present in appreciable populations. When SAM is noncovalently bound within enzyme active sites, the nature and the number of different conformations of the molecule are likely to be altered from when it is free in solution. If there are unique structures or different numbers of conformers between different methyltransferase active sites, solution-state information may provide promising structural leads to increase inhibitor specificity for a particular methyltransferase. Toward this goal, frequencies measured in SAM's infrared spectra must be assigned to the motions of specific atoms via isotope incorporation at discrete positions. The incorporation of isotopes into SAM's structure can be accomplished via an established enzymatic synthesis using isotopically labeled precursors. However, published protocols produced an intense and highly variable IR signal which overlapped with many of the signals from SAM rendering comparison between isotopes challenging. We observed this intense absorption to be from co-purifying salts and the SAM counterion, producing a strong, broad signal at 1100 cm |
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| MeSH term(s) | Methionine ; S-Adenosylmethionine/chemistry ; S-Adenosylmethionine/metabolism ; Salts ; Methyltransferases/chemistry ; Methyltransferases/metabolism ; Racemethionine ; Isotopes |
| Chemical Substances | Methionine (AE28F7PNPL) ; S-Adenosylmethionine (7LP2MPO46S) ; Salts ; Methyltransferases (EC 2.1.1.-) ; Racemethionine (73JWT2K6T3) ; Isotopes |
| Language | English |
| Publishing date | 2024-01-02 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 210413-1 |
| ISSN | 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425 |
| ISSN (online) | 1873-3557 |
| ISSN | 0370-8322 ; 0584-8539 ; 1386-1425 |
| DOI | 10.1016/j.saa.2023.123816 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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