Article ; Online: Andrographolide protects against doxorubicin-and arsenic trioxide-induced toxicity in cardiomyocytes
Mol Biol Rep. 2023 Jan., v. 50, no. 1 p.389-397
2023
Abstract: BACKGROUND: Andrographolide (AG) is a lactone diterpene with valuable biological activities. This in vitro study evaluated whether AG can protect cardiomyocytes under toxicities triggered with anti-cancer chemotherapeutic agents, doxorubicin (DOX) and ... ...
| Abstract | BACKGROUND: Andrographolide (AG) is a lactone diterpene with valuable biological activities. This in vitro study evaluated whether AG can protect cardiomyocytes under toxicities triggered with anti-cancer chemotherapeutic agents, doxorubicin (DOX) and arsenic trioxide (ATO). METHODS AND RESULTS: H9C2 cells were pretreated with AG (0.5–10 µM) for 24 h and then exposed to DOX (1 μM) or ATO (35 μM) for another 24 h period. For determination of cell viability or cytotoxicity, MTT and lactate dehydrogenase (LDH) assay were used. Total oxidant and antioxidant capacities were estimated by determining hydroperoxides and ferric reducing antioxidant power (FRAP) levels. Real time-polymerase chain reaction was also used for quantitative evaluation of TLR4 gene expression. AG inhibited cardiomyocytes proliferation at the concentrations of more than 20 μM. However, it considerably enhanced cell viability and decreased cytotoxicity of DOX and ATO at the concentration range of 2.5–10 μM in MTT and LDH assays. AG significantly declined hydroperoxides concentration in ATO-treated cardiomyocytes and raised FRAP value in DOX- and ATO-treated cells. Furthermore, AG notably lessened TLR4 expression in H9C2 cells after exposure to DOX- and ATO. CONCLUSION: In conclusion, these data presented that AG was able to reverse DOX- and ATO-induced cardiotoxicity in vitro. The cardiomyocyte protective activities of AG may be due to the decrease in TLR4 expression and total oxidant capacity and increase in total antioxidant capacity. |
|---|---|
| Keywords | andrographolide ; antioxidant activity ; arsenic ; arsenic oxide ; cardiomyocytes ; cardiotoxicity ; cell viability ; cytotoxicity ; doxorubicin ; drug therapy ; gene expression ; hydroperoxides ; lactate dehydrogenase ; oxidants ; quantitative analysis |
| Language | English |
| Dates of publication | 2023-01 |
| Size | p. 389-397. |
| Publishing place | Springer Netherlands |
| Document type | Article ; Online |
| ZDB-ID | 186544-4 |
| ISSN | 1573-4978 ; 0301-4851 |
| ISSN (online) | 1573-4978 |
| ISSN | 0301-4851 |
| DOI | 10.1007/s11033-022-08042-4 |
| Database | NAL-Catalogue (AGRICOLA) |
Full text online
More links
Kategorien
In stock of ZB MED Bonn / Germany
| Z 78/728: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.