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Article ; Online: Editorial

Tao Li / Haihong Hao / Xiaolin Hou / Jiang Xia

Frontiers in Microbiology, Vol

Antimicrobial resistance: agriculture, environment and public health within One Health framework

2023 Volume 14

Keywords	antimicrobial resistance ; agriculture ; public health ; One Health ; ecosystem ; drug resistance ; Microbiology ; QR1-502
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Clinical Breakpoint of Apramycin to Swine Salmonell a and Its Effect on Ileum Flora

Xinyu Dai / Yufeng Gu / Jinli Guo / Lingli Huang / Guyue Cheng / Dapeng Peng / Haihong Hao

International Journal of Molecular Sciences, Vol 23, Iss 1424, p

2022 Volume 1424

Abstract: The purpose of this study was to establish the clinical breakpoint (CBP) of apramycin (APR) against Salmonella in swine and evaluate its effect on intestinal microbiota. The CBP was established based on three cutoff values of wild-type cutoff value (CO ... ...

Abstract	The purpose of this study was to establish the clinical breakpoint (CBP) of apramycin (APR) against Salmonella in swine and evaluate its effect on intestinal microbiota. The CBP was established based on three cutoff values of wild-type cutoff value (CO WT ), pharmacokinetic-pharmadynamic (PK/PD) cutoff value (CO PD ) and clinical cutoff value (CO CL ). The effect of the optimized dose regimen based on ex vivo PK/PD study. The evolution of the ileum flora was determined by the 16rRNA gene sequencing and bioinformatics. This study firstly established the CO WT , CO PD in ileum, and CO CL of APR against swine Salmonella , the value of these cutoffs were 32 µg/mL, 32 µg/mL and 8 µg/mL, respectively. According to the guiding principle of the Clinical Laboratory Standards Institute (CLSI), the final CBP in ileum was 32 µg/mL. Our results revealed the main evolution route in the composition of ileum microbiota of diarrheic piglets treated by APR. The change of the abundances of Bacteroidetes and Euryarchaeota was the most obvious during the evolution process. Methanobrevibacter , Prevotella , S24-7 and Ruminococcaceae were obtained as the highest abundance genus. The abundance of Methanobrevibacter increased significantly when APR treatment carried and decreased in cure and withdrawal period groups. The abundance of Prevotella in the tested groups was significantly lower than that in the healthy group. A decreased of abundance in S24-7 was observed after Salmonella infection and increased slightly after cure. Ruminococcaceae increased significantly after Salmonella infection and decreased significantly after APR treatment. In addition, the genera of Methanobrevibacter and Prevotella were defined as the key node. Valine, leucine and isoleucine biosynthesis, D-Glutamine and D-glutamate metabolism, D-Alanine metabolism, Peptidoglycan and amino acids biosynthesis were the top five Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in the ileum microbiota of piglets during the Salmonella infection and APR treatment ...
Keywords	Salmonella ; apramycin ; wild-type cutoff value ; PK/PD cutoff value ; clinical cutoff value ; clinical breakpoint ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	630
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Phage Products for Fighting Antimicrobial Resistance

Yuanling Huang / Wenhui Wang / Zhihao Zhang / Yufeng Gu / Anxiong Huang / Junhao Wang / Haihong Hao

Microorganisms, Vol 10, Iss 7, p

2022 Volume 1324

Abstract: Antimicrobial resistance (AMR) has become a global public health issue and antibiotic agents have lagged behind the rise in bacterial resistance. We are searching for a new method to combat AMR and phages are viruses that can effectively fight bacterial ... ...

Abstract	Antimicrobial resistance (AMR) has become a global public health issue and antibiotic agents have lagged behind the rise in bacterial resistance. We are searching for a new method to combat AMR and phages are viruses that can effectively fight bacterial infections, which have renewed interest as antibiotic alternatives with their specificity. Large phage products have been produced in recent years to fight AMR. Using the “one health” approach, this review summarizes the phage products used in plant, food, animal, and human health. In addition, the advantages and disadvantages and future perspectives for the development of phage therapy as an antibiotic alternative to combat AMR are also discussed in this review.
Keywords	antimicrobial resistance ; phage products ; advantages and disadvantages of phage therapy ; development prospects ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Optimal regimens based on PK/PD cutoff evaluation of ceftiofur against Actinobacillus pleuropneumoniae in swine

Da Sun / Kun Mi / Haihong Hao / Shuyu Xie / Dongmei Chen / Lingli Huang

BMC Veterinary Research, Vol 16, Iss 1, Pp 1-

2020 Volume 11

Abstract: Abstract Background Actinobacillus pleuropneumoniae formerly known as Haemophilus pleuropneumoniae, can cause pleuropneumoniae in pigs, which lead to significant mortality. Ceftiofur was the first cephalosporin antibiotic used in animals, which was ... ...

Abstract	Abstract Background Actinobacillus pleuropneumoniae formerly known as Haemophilus pleuropneumoniae, can cause pleuropneumoniae in pigs, which lead to significant mortality. Ceftiofur was the first cephalosporin antibiotic used in animals, which was effective against gram-negative and gram-positive bacterium. This study aimed to formulate a rational dosage strategy and review the preceding recommended dosage based on PK/PD modeling and Establish Clinical breakpoint of ceftiofur against Actinobacillus pleuropneumoniae based on the pharmacodynamic-pharmacokinetic cutoff. Results The epidemiologic cutoff value was 0.125 μg/mL. The results of the pharmacodynamic study showed that the MICs of BW39 were 0.5 μg/mL and 1 μg/mL in vitro and ex-vivo, respectively. The minimal bactericidal concentrations (MBCs) under in vitro and ex vivo conditions were both 1 μg/mL. The time-killing profiles of ceftiofur against BW39 were time-dependent with a partly concentration-dependent pattern. Based on the inhibitory sigmoid Emax model, the AUC24 h/MIC values for the bacteriostatic, bactericidal, and elimination effects in serum were 45.73, 63.83, and 69.04 h for healthy pigs separately. According to the Monte Carlo simulation, the COPD was calculated as 2 μg/mL, and the optimized dosage regimen of ceftiofur against Actinobacillus pleuropneumoniae to achieve bacteriostatic, bactericidal, and elimination effects over 24 h was 2.13, 2.97, and 3.42 mg/kg for the 50% target attainment rate (TAR) and 2.47, 3.21, and 3.70 mg/kg for the 90% TAR respectively. Conclusions In conclusion, we reveal the EOFF and PK/PD cutoff values of ceftiofur against A. pleuropneumoniae in piglets. However, with the paucity of clinical data for ceftiofur to establish a clinical cutoff against A. pleuropneumoniae, the PK/PD cutoff value of 2 μg/mL will be recommended as surrogate. According to the PK/PD data and the MIC distribution in China, the single bactericidal dose was 3.21 mg/kg for the 90% target, which would be more able to cure Actinobacillus ...
Keywords	Ceftiofur ; Actinobacillus pleuropneumoniae ; Epidemiologic cutoff value ; Pharmacokinetic/pharmacodynamics cutoff ; Dosage ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Evidence for Establishing the Clinical Breakpoint of Cefquinome against Haemophilus Parasuis in China

Kun Mi / Da Sun / Mei Li / Haihong Hao / Kaixiang Zhou / Zhenli Liu / Zonghui Yuan / Lingli Huang

Pathogens, Vol 10, Iss 2, p

2021 Volume 105

Abstract: Haemophilus parasuis can cause high morbidity and mortality in swine. Cefquinome possesses excellent antibacterial activity against pathogens causing diseases of the respiratory tract. This study aimed to establish the clinical breakpoint (CBP) of ... ...

Abstract	Haemophilus parasuis can cause high morbidity and mortality in swine. Cefquinome possesses excellent antibacterial activity against pathogens causing diseases of the respiratory tract. This study aimed to establish the clinical breakpoint (CBP) of cefquinome against H. parasuis and to monitor the resistance change. Referring to the minimum inhibitory concentration (MIC) distribution of cefquinome against 131 H. parasuis isolates, the MIC 50 and MIC 90 were determined to be 0.125 and 1 μg/mL, respectively. And the epidemiological cutoff (ECOFF) value was 1 μg/mL. HPS42 was selected as a representative strain for the pharmacodynamic (PD) experiment, pharmacokinetic (PK) experiment and clinical experiments. The PK/PD index values, area under concentration-time curve (AUC)/MIC, of the bacteriostatic, bactericidal, and bacterial elimination effects were 23, 41, and 51 h, respectively. The PK/PD cutoff was calculated as 0.125 μg/mL by Monte Carlo simulation (MCS), and the clinical cutoff was 0.25−4 μg/mL by WindoW. Combing these three values, the CBP of cefquinome against H. parasuis was found to be 1 μg/mL. In conclusion, this was the first study to integrate various cutoffs to establish the CBP in the laboratory. It is helpful to distinguish wild type H. parasuis and reduce the probability of treatment failure.
Keywords	clinical breakpoint ; epidemiological cutoff ; PK/PD cutoff ; clinical cutoff ; Haemophilus parasuis ; cefquinome ; Medicine ; R
Subject code	610
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Corrigendum to “Application of PK/PD Modeling in Veterinary Field

Ijaz Ahmad / Lingli Huang / Haihong Hao / Pascal Sanders / Zonghui Yuan

BioMed Research International, Vol

Dose Optimization and Drug Resistance Prediction”

2017 Volume 2017

Keywords	Medicine ; R
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Applications of new functions for inducing host defense peptides and synergy sterilization of medium chain fatty acids in substituting in-feed antibiotics

Zhou, Zhongxin / Haihong Hao / Hongkui Wei / Jian Peng / Jie Huang / Yuanfei Zhou

Journal of functional foods. 2019 Jan., v. 52

2019

Abstract: Developing alternatives to classic antibiotic growth promoters (AGPs) in feed industry has been being urgently called but an individual alternative is unlikely to embody all functions of classic antibiotics. One potential solution is to select multiple ... ...

Abstract	Developing alternatives to classic antibiotic growth promoters (AGPs) in feed industry has been being urgently called but an individual alternative is unlikely to embody all functions of classic antibiotics. One potential solution is to select multiple products which work synergistically and a comprehensive solution. This review mainly discuss recent advances in medium-chain fatty acids’ (MCFAs) roles as an important component of the mammalian innate immune defense system, new functions for inducing host defense peptides and synergy sterilizations with edible essential oils or edible organic acids, and these multifaceted functions’ application potential to solve their technological challenges in substituting in-feed antibiotics such as unpleasant odor, low solubility, poor chemical stability and volatile nature. The review highlights that MCFAs are originally an important component of innate immune defense system in mammalian breast milk, skin and mucosa, and also induce host defense peptide expression in human, pig, chicken, rabbit and mice, which are beneficial supplement to their previously found in vitro antimicrobial functions. It is concluded that using encapsulated MCFAs combinations with edible essential oils or organic acids would be one preferred solution to overcome their technological challenges in substituting in-feed antibiotics such as unpleasant odor, low solubility, poor chemical stability and volatile nature, and also have the relative difficulty with which bacteria develop resistance. However, the basic data related to combinations of various alternatives and the underlying mechanisms remain lacking.
Keywords	antibiotics ; bacteria ; breast milk ; chickens ; essential oils ; feed industry ; growth promotion ; humans ; innate immunity ; medium chain fatty acids ; mice ; mucosa ; odors ; peptides ; rabbits ; solubility ; swine
Language	English
Dates of publication	2019-01
Size	p. 348-359.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2511964-3
ISSN	1756-4646
ISSN	1756-4646
DOI	10.1016/j.jff.2018.11.028
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Application of PK/PD Modeling in Veterinary Field

Ijaz Ahmad / Lingli Huang / Haihong Hao / Pascal Sanders / Zonghui Yuan

BioMed Research International, Vol

Dose Optimization and Drug Resistance Prediction

2016 Volume 2016

Abstract: Among veterinary drugs, antibiotics are frequently used. The true mean of antibiotic treatment is to administer dose of drug that will have enough high possibility of attaining the preferred curative effect, with adequately low chance of concentration ... ...

Abstract	Among veterinary drugs, antibiotics are frequently used. The true mean of antibiotic treatment is to administer dose of drug that will have enough high possibility of attaining the preferred curative effect, with adequately low chance of concentration associated toxicity. Rising of antibacterial resistance and lack of novel antibiotic is a global crisis; therefore there is an urgent need to overcome this problem. Inappropriate antibiotic selection, group treatment, and suboptimal dosing are mostly responsible for the mentioned problem. One approach to minimizing the antibacterial resistance is to optimize the dosage regimen. PK/PD model is important realm to be used for that purpose from several years. PK/PD model describes the relationship between drug potency, microorganism exposed to drug, and the effect observed. Proper use of the most modern PK/PD modeling approaches in veterinary medicine can optimize the dosage for patient, which in turn reduce toxicity and reduce the emergence of resistance. The aim of this review is to look at the existing state and application of PK/PD in veterinary medicine based on in vitro, in vivo, healthy, and disease model.
Keywords	Medicine ; R
Language	English
Publishing date	2016-01-01T00:00:00Z
Publisher	Hindawi Publishing Corporation
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Inter-Relationship Between a Transcriptional Regulator of Flagella Genes cj0440c and Thiamine Metabolic Pathway in Campylobacter jejuni

Muhammad Abu Bakr Shabbir / Aziz Ul-Rahman / Abdur Rauf Khalid / Nabeel Ijaz / Muhammmad Tahir Aleem / Saeed Ahmed / Abdulaziz Alouffi / Waqas Ahmed / Faiza Aslam / Muhammad Kashif Maan / Adnan Hassan Tahir / Muhammad Waqar Aziz / Mashal M. Almutairi / Haihong Hao

BioMed Research International, Vol

2022 Volume 2022

Abstract: Campylobacter jejuni is a major cause of gastroenteritis in humans. It has been reported that the pathogenesis of C. jejuni is closely related to the formation, adhesion, and invasion of flagella toxin in host epithelial cells. A putative transcriptional ...

Abstract	Campylobacter jejuni is a major cause of gastroenteritis in humans. It has been reported that the pathogenesis of C. jejuni is closely related to the formation, adhesion, and invasion of flagella toxin in host epithelial cells. A putative transcriptional regulator, known as cj0440c, is thought to be involved in the regulation of flagellar synthesis. However, confirmation of this hypothesis requires deep insight into the regulation mechanism of cj0440c and its possible relationship with different antibiotics. Therefore, the study explained here was designed to determine the relationship and function (phenotypically and genotypically) of cj0440c in the flagellar synthesis of C. jejuni NCTC11168. The study determined the mode of expression of cj0440c and flagella-related genes under exposure to various drugs. To verify the involvement of cj0440c protein in the metabolic pathway of thiamine, an enzymatic hydrolysis experiment was performed and analyzed through the application of mass spectrometry. The overexpression vector of C. jejuni NCTC11168 was also constructed to find out whether or not target genes were regulated by cj0440c. The findings of the study showed that cj0440c and other flagella-related genes were expressed differentially under the influence of various antibiotics including erythromycin, tylosin, azithromycin, gentamicin, etimicin, enrofloxacin, gatifloxacin, tetracycline, and tigecycline. The analysis showed that the cj0440c protein did not catalyze the degradation of thiamine. In conclusion, the study aids in the understanding of the inter-relationship between the regulatory mechanism of flagella genes and the thiamine metabolic pathway.
Keywords	Medicine ; R
Subject code	570
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effects of Acute and Chronic Exposure to Residual Level Erythromycin on Human Intestinal Epithelium Cell Permeability and Cytotoxicity

Haihong Hao / Kuppan Gokulan / Silvia A. Piñeiro / Katherine M. Williams / Zonghui Yuan / Carl E. Cerniglia / Sangeeta Khare

Microorganisms, Vol 7, Iss 9, p

2019 Volume 325

Abstract: Residual concentrations of erythromycin in food could result in gastrointestinal tract exposure that potentially poses a health-hazard to the consumer, affecting intestinal epithelial permeability, barrier function, microbiota composition, and ... ...

Abstract	Residual concentrations of erythromycin in food could result in gastrointestinal tract exposure that potentially poses a health-hazard to the consumer, affecting intestinal epithelial permeability, barrier function, microbiota composition, and antimicrobial resistance. We investigated the effects of erythromycin after acute (48 h single treatment with 0.03 μg/mL to 300 μg/mL) or chronic (repeated treatment with 0.3 µg/mL and 300 µg/mL erythromycin for five days) exposures on the permeability of human colonic epithelial cells, a model that mimics a susceptible intestinal surface devoid of commensal microbiota. Transepithelial electrical resistance (TER) measurements indicated that erythromycin above 0.3 µg/mL may compromise the epithelial barrier. Acute exposure increased cytotoxicity, while chronic exposure decreased the cytotoxicity. Quantitative PCR analysis revealed that only ICAM1 (intercellular adhesion molecule 1) was up-regulated during 0.3 μg/mL acute-exposure, while ICAM1 , JAM3 (junctional adhesion molecule 3), and ITGA8 (integrin alpha 8), were over-expressed in the 300 μg/mL acute treatment group. However, during chronic exposure, no change in the mRNA expression was observed at 0.3 μg/mL, and only ICAM2 was significantly up-regulated after 300 μg/mL. ICAM1 and ICAM2 are known to be involved in the formation of extracellular matrices. These gene expression changes may be related to the immunoregulatory activity of erythromycin, or a compensatory mechanism of the epithelial cells to overcome the distress caused by erythromycin due to increased permeability.
Keywords	erythromycin ; epithelial cell barrier function ; gene expression ; acute or multiple dose exposure ; permeability ; single dose exposure ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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