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  1. AU="Haist, Kelsey C"
  2. AU="Lin, Jeremy"
  3. AU="Saeed, Maria"
  4. AU="Benmeziane, Keltouma"
  5. AU="Barik, Saroj K."
  6. AU="Madhurima Basu" AU="Madhurima Basu"
  7. AU="Polanska, Nikola"
  8. AU="Stephane Cullati"
  9. AU="Held, Noelle A"
  10. AU="Zhu, Simeng"
  11. AU="Hantour, Naima"
  12. AU="Bo Shopsin"
  13. AU="Reese, Samario"
  14. AU="Retamal, Catalina"
  15. AU="Lee-Wing, Matthew"
  16. AU="Dostálová, I"
  17. AU=McCloskey Kayleigh A
  18. AU="Dharmarajan, Arun"
  19. AU="Stebel, Luigi"
  20. AU=Amico Patrizia
  21. AU="Kojev, Aslan"
  22. AU="Zhiyu Wang"
  23. AU="Shannon C Peyton"
  24. AU="Shiltsev, V."
  25. AU="Edward S. Debnam"
  26. AU="Freeston, Sarah L"
  27. AU="Bertolucci, S."
  28. AU="de Barros, Rosires M B"
  29. AU="Carr, Crystal C"
  30. AU="Davies, Mark Lloyd"
  31. AU=St Gelais Corine
  32. AU=Engstrom Malitta
  33. AU="Hongo, Akane"
  34. AU="Krykorková, I"
  35. AU=Yan Bing
  36. AU="Nakos, Konstantinos"
  37. AU="Schreiner, Ryan"
  38. AU=Pltz T
  39. AU="Akhmanova, Anna" AU="Akhmanova, Anna"
  40. AU="Goretsky, Anton"
  41. AU="Cordoza, Makayla L"
  42. AU=Midoux Patrick AU=Midoux Patrick
  43. AU="Mundt, H M"
  44. AU=Tsivitse Susan

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  1. Artikel ; Online: TNFα: TNFR1 signaling inhibits maturation and maintains the pro-inflammatory programming of monocyte-derived macrophages in murine chronic granulomatous disease.

    Gibbings, Sophie L / Haist, Kelsey C / Redente, Elizabeth F / Henson, Peter M / Bratton, Donna L

    Frontiers in immunology

    2024  Band 15, Seite(n) 1354836

    Abstract: Introduction: Loss of NADPH oxidase activity results in proinflammatory macrophages that contribute to hyperinflammation in Chronic Granulomatous Disease (CGD). Previously, it was shown in a zymosan-induced peritonitis model that gp91: Methods: We ... ...

    Abstract Introduction: Loss of NADPH oxidase activity results in proinflammatory macrophages that contribute to hyperinflammation in Chronic Granulomatous Disease (CGD). Previously, it was shown in a zymosan-induced peritonitis model that gp91
    Methods: We sought to identify key constituents using
    Results: More extensive phenotyping defined normal MoMac maturation and demonstrated failure of maturation of CGD MoMacs both
    Discussion: These data lend mechanistic insights into the utility of TNFα blockade in CGD and to other diseases where such therapy has been shown to be beneficial.
    Mesh-Begriff(e) Animals ; Mice ; Culture Media, Conditioned/metabolism ; Cytokines/metabolism ; Granulomatous Disease, Chronic/therapy ; Macrophages/metabolism ; NADPH Oxidases/metabolism ; Receptors, Tumor Necrosis Factor, Type I/genetics ; Receptors, Tumor Necrosis Factor, Type I/metabolism ; Tumor Necrosis Factor-alpha/metabolism
    Chemische Substanzen Culture Media, Conditioned ; Cytokines ; NADPH Oxidases (EC 1.6.3.-) ; Receptors, Tumor Necrosis Factor, Type I ; Tumor Necrosis Factor-alpha ; Tnfrsf1a protein, mouse
    Sprache Englisch
    Erscheinungsdatum 2024-02-09
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2024.1354836
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: A LTB

    Haist, Kelsey C / Gibbings, Sophie L / Jacobelli, Jordan / Mould, Kara J / Henson, Peter M / Bratton, Donna L

    iScience

    2024  Band 27, Heft 4, Seite(n) 109589

    Abstract: Sterile pyogranulomas and heightened cytokine production are hyperinflammatory hallmarks of Chronic Granulomatous Disease (CGD). Using peritoneal cells of zymosan-treated CGD ( ... ...

    Abstract Sterile pyogranulomas and heightened cytokine production are hyperinflammatory hallmarks of Chronic Granulomatous Disease (CGD). Using peritoneal cells of zymosan-treated CGD (gp91
    Sprache Englisch
    Erscheinungsdatum 2024-03-27
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2024.109589
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Plasmacytoid Dendritic Cells Mediate Control of Ross River Virus Infection via a Type I Interferon-Dependent, MAVS-Independent Mechanism.

    Haist, Kelsey C / Carpentier, Kathryn S / Davenport, Bennett J / Morrison, Thomas E

    Journal of virology

    2021  Band 95, Heft 6

    Abstract: Ross River virus (RRV) is a mosquito-borne alphavirus that causes epidemics of debilitating musculoskeletal disease. To define the innate immune mechanisms that mediate control of RRV infection, we studied a RRV strain encoding 6 nonsynonymous mutations ... ...

    Abstract Ross River virus (RRV) is a mosquito-borne alphavirus that causes epidemics of debilitating musculoskeletal disease. To define the innate immune mechanisms that mediate control of RRV infection, we studied a RRV strain encoding 6 nonsynonymous mutations in nsP1 (RRV-T48-nsP1
    Mesh-Begriff(e) Adaptor Proteins, Signal Transducing/deficiency ; Adaptor Proteins, Signal Transducing/metabolism ; Alphavirus Infections/immunology ; Alphavirus Infections/virology ; Animals ; Antiviral Agents/metabolism ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Immunity, Innate ; Interferon Type I/metabolism ; Mice ; Mutation ; Ross River virus/genetics ; Ross River virus/pathogenicity ; Signal Transduction ; Viral Load ; Viral Nonstructural Proteins/genetics ; Virulence/genetics
    Chemische Substanzen Adaptor Proteins, Signal Transducing ; Antiviral Agents ; IPS-1 protein, mouse ; Interferon Type I ; Viral Nonstructural Proteins
    Sprache Englisch
    Erscheinungsdatum 2021-02-24
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.01538-20
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

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  4. Artikel ; Online: Inflammatory monocytes mediate control of acute alphavirus infection in mice.

    Haist, Kelsey C / Burrack, Kristina S / Davenport, Bennett J / Morrison, Thomas E

    PLoS pathogens

    2017  Band 13, Heft 12, Seite(n) e1006748

    Abstract: Chikungunya virus (CHIKV) and Ross River virus (RRV) are mosquito-transmitted alphaviruses that cause debilitating acute and chronic musculoskeletal disease. Monocytes are implicated in the pathogenesis of these infections; however, their specific roles ... ...

    Abstract Chikungunya virus (CHIKV) and Ross River virus (RRV) are mosquito-transmitted alphaviruses that cause debilitating acute and chronic musculoskeletal disease. Monocytes are implicated in the pathogenesis of these infections; however, their specific roles are not well defined. To investigate the role of inflammatory Ly6ChiCCR2+ monocytes in alphavirus pathogenesis, we used CCR2-DTR transgenic mice, enabling depletion of these cells by administration of diptheria toxin (DT). DT-treated CCR2-DTR mice displayed more severe disease following CHIKV and RRV infection and had fewer Ly6Chi monocytes and NK cells in circulation and muscle tissue compared with DT-treated WT mice. Furthermore, depletion of CCR2+ or Gr1+ cells, but not NK cells or neutrophils alone, restored virulence and increased viral loads in mice infected with an RRV strain encoding attenuating mutations in nsP1 to levels detected in monocyte-depleted mice infected with fully virulent RRV. Disease severity and viral loads also were increased in DT-treated CCR2-DTR+;Rag1-/- mice infected with the nsP1 mutant virus, confirming that these effects are independent of adaptive immunity. Monocytes and macrophages sorted from muscle tissue of RRV-infected mice were viral RNA positive and had elevated expression of Irf7, and co-culture of Ly6Chi monocytes with RRV-infected cells resulted in induction of type I IFN gene expression in monocytes that was Irf3;Irf7 and Mavs-dependent. Consistent with these data, viral loads of the attenuated nsP1 mutant virus were equivalent to those of WT RRV in Mavs-/- mice. Finally, reconstitution of Irf3-/-;Irf7-/- mice with CCR2-DTR bone marrow rescued mice from severe infection, and this effect was reversed by depletion of CCR2+ cells, indicating that CCR2+ hematopoietic cells are capable of inducing an antiviral response. Collectively, these data suggest that MAVS-dependent production of type I IFN by monocytes is critical for control of acute alphavirus infection and that determinants in nsP1, the viral RNA capping protein, counteract this response.
    Mesh-Begriff(e) Adaptor Proteins, Signal Transducing/deficiency ; Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/immunology ; Alphavirus Infections/immunology ; Alphavirus Infections/virology ; Animals ; Antigens, Ly/metabolism ; Chikungunya virus/immunology ; Chikungunya virus/pathogenicity ; Diphtheria Toxin/pharmacology ; Heparin-binding EGF-like Growth Factor/genetics ; Heparin-binding EGF-like Growth Factor/immunology ; Humans ; Inflammation/virology ; Interferon Regulatory Factor-3/deficiency ; Interferon Regulatory Factor-3/genetics ; Interferon Regulatory Factor-3/immunology ; Interferon Regulatory Factor-7/deficiency ; Interferon Regulatory Factor-7/genetics ; Interferon Regulatory Factor-7/immunology ; Interferon Type I/biosynthesis ; Interferon Type I/genetics ; Killer Cells, Natural/drug effects ; Killer Cells, Natural/immunology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Monocytes/drug effects ; Monocytes/immunology ; Monocytes/virology ; Receptors, CCR2/genetics ; Receptors, CCR2/metabolism ; Ross River virus/genetics ; Ross River virus/immunology ; Ross River virus/pathogenicity ; Viral Load ; Virulence/genetics ; Virulence/immunology
    Chemische Substanzen Adaptor Proteins, Signal Transducing ; Antigens, Ly ; Ccr2 protein, mouse ; Diphtheria Toxin ; Heparin-binding EGF-like Growth Factor ; IPS-1 protein, mouse ; Interferon Regulatory Factor-3 ; Interferon Regulatory Factor-7 ; Interferon Type I ; Irf3 protein, mouse ; Irf7 protein, mouse ; Ly-6C antigen, mouse ; Receptors, CCR2
    Sprache Englisch
    Erscheinungsdatum 2017
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7366
    ISSN (online) 1553-7374
    ISSN 1553-7366
    DOI 10.1371/journal.ppat.1006748
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Heightened turnover and failed maturation of monocyte-derived macrophages in murine chronic granulomatous disease.

    Gibbings, Sophie L / Haist, Kelsey C / Nick, Heidi / Frasch, S Courtney / Glass, Teagan H / Vestal, Brian / Danhorn, Thomas / Mould, Kara J / Henson, Peter M / Bratton, Donna L

    Blood

    2021  Band 139, Heft 11, Seite(n) 1707–1721

    Abstract: Loss of NADPH oxidase activity leads to altered phagocyte responses and exaggerated inflammation in chronic granulomatous disease (CGD). We sought to assess the effects of Nox2 absence on monocyte-derived macrophages (MoMacs) in gp91phox-/y mice during ... ...

    Abstract Loss of NADPH oxidase activity leads to altered phagocyte responses and exaggerated inflammation in chronic granulomatous disease (CGD). We sought to assess the effects of Nox2 absence on monocyte-derived macrophages (MoMacs) in gp91phox-/y mice during zymosan-induced peritonitis. MoMacs from CGD and wild-type (WT) peritonea were characterized over time after zymosan injection. Although numbers lavaged from both genotypes were virtually identical, there were marked differences in maturation: newly recruited WT MoMacs rapidly enlarged and matured, losing Ly6C and gaining MHCII, CD206, and CD36, whereas CGD MoMacs remained small and were mostly Ly6C+MHCII-. RNA-sequencing analyses showed few intrinsic differences between genotypes in newly recruited MoMacs but significant differences with time. WT MoMacs displayed changes in metabolism, adhesion, and reparative functions, whereas CGD MoMacs remained inflammatory. PKH dye labeling revealed that although WT MoMacs were mostly recruited within the first 24 hours and remained in the peritoneum while maturing and enlarging, CGD monocytes streamed into the peritoneum for days, with many migrating to the diaphragm where they were found in fibrin(ogen) clots surrounding clusters of neutrophils in nascent pyogranulomata. Importantly, these observations seemed to be driven by milieu: adoptive transfer of CGD MoMacs into inflamed peritonea of WT mice resulted in immunophenotypic maturation and normal behavior, whereas altered maturation/behavior of WT MoMacs resulted from transfer into inflamed peritonea of CGD mice. In addition, Nox2-deficient MoMacs behaved similarly to their Nox2-sufficient counterparts within the largely WT milieu of mixed bone marrow chimeras. These data show persistent recruitment with fundamental failure of MoMac maturation in CGD.
    Mesh-Begriff(e) Animals ; Granulomatous Disease, Chronic/genetics ; Inflammation/metabolism ; Macrophages/metabolism ; Mice ; NADPH Oxidases/genetics ; NADPH Oxidases/metabolism ; Neutrophils/metabolism
    Chemische Substanzen NADPH Oxidases (EC 1.6.3.-)
    Sprache Englisch
    Erscheinungsdatum 2021-10-25
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood.2021011798
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Uh III Zs.140: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 364: Hefte anzeigen

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  6. Artikel ; Online: dsRNA-Seq: Identification of Viral Infection by Purifying and Sequencing dsRNA

    Decker, Carolyn J. / Steiner, Halley R. / Hoon-Hanks, Laura L. / Morrison, James H. / Haist, Kelsey C. / Stabell, Alex C. / Poeschla, Eric M. / Morrison, Thomas E. / Stenglein, Mark D. / Sawyer, Sara L. / Parker, Roy

    Viruses. 2019 Oct. 14, v. 11, no. 10

    2019  

    Abstract: RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that RNA viruses produce double-stranded RNA (dsRNA) while replicating. Purifying and ...

    Abstract RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that RNA viruses produce double-stranded RNA (dsRNA) while replicating. Purifying and sequencing dsRNA from the total RNA isolated from infected tissue allowed us to recover dsRNA virus sequences and replicated sequences from single-stranded RNA (ssRNA) viruses. We refer to this approach as dsRNA-Seq. By assembling dsRNA sequences into contigs we identified full length or partial RNA viral genomes of varying genome types infecting mammalian culture samples, identified a known viral disease agent in laboratory infected mice, and successfully detected naturally occurring RNA viral infections in reptiles. Here, we show that dsRNA-Seq is a preferable method for identifying viruses in organisms that don’t have sequenced genomes and/or commercially available rRNA depletion reagents. In addition, a significant advantage of this method is the ability to identify replicated viral sequences of ssRNA viruses, which is useful for distinguishing infectious viral agents from potential noninfectious viral particles or contaminants.
    Schlagwörter animal diseases ; double-stranded RNA ; genome ; infectious diseases ; mice ; reptiles ; ribosomal RNA ; sequence analysis ; ssRNA viruses ; virion ; viruses
    Sprache Englisch
    Erscheinungsverlauf 2019-1014
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel ; Online
    Anmerkung Resource is Open Access
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v11100943
    Datenquelle NAL Katalog (AGRICOLA)

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  7. Artikel ; Online: dsRNA-Seq: Identification of Viral Infection by Purifying and Sequencing dsRNA.

    Decker, Carolyn J / Steiner, Halley R / Hoon-Hanks, Laura L / Morrison, James H / Haist, Kelsey C / Stabell, Alex C / Poeschla, Eric M / Morrison, Thomas E / Stenglein, Mark D / Sawyer, Sara L / Parker, Roy

    Viruses

    2019  Band 11, Heft 10

    Abstract: RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that RNA viruses produce double-stranded RNA (dsRNA) while replicating. Purifying and ...

    Abstract RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that RNA viruses produce double-stranded RNA (dsRNA) while replicating. Purifying and sequencing dsRNA from the total RNA isolated from infected tissue allowed us to recover dsRNA virus sequences and replicated sequences from single-stranded RNA (ssRNA) viruses. We refer to this approach as dsRNA-Seq. By assembling dsRNA sequences into contigs we identified full length or partial RNA viral genomes of varying genome types infecting mammalian culture samples, identified a known viral disease agent in laboratory infected mice, and successfully detected naturally occurring RNA viral infections in reptiles. Here, we show that dsRNA-Seq is a preferable method for identifying viruses in organisms that don't have sequenced genomes and/or commercially available rRNA depletion reagents. In addition, a significant advantage of this method is the ability to identify replicated viral sequences of ssRNA viruses, which is useful for distinguishing infectious viral agents from potential noninfectious viral particles or contaminants.
    Mesh-Begriff(e) Animals ; Chlorocebus aethiops ; Genome, Viral ; High-Throughput Nucleotide Sequencing ; Mice ; RNA Virus Infections/virology ; RNA Viruses/genetics ; RNA Viruses/isolation & purification ; RNA, Double-Stranded/isolation & purification ; RNA, Viral/isolation & purification ; RNA-Seq ; Vero Cells ; Virion ; Virus Replication
    Chemische Substanzen RNA, Double-Stranded ; RNA, Viral
    Sprache Englisch
    Erscheinungsdatum 2019-10-14
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v11100943
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Discrete viral E2 lysine residues and scavenger receptor MARCO are required for clearance of circulating alphaviruses.

    Carpentier, Kathryn S / Davenport, Bennett J / Haist, Kelsey C / McCarthy, Mary K / May, Nicholas A / Robison, Alexis / Ruckert, Claudia / Ebel, Gregory D / Morrison, Thomas E

    eLife

    2019  Band 8

    Abstract: The magnitude and duration of vertebrate viremia is a critical determinant of arbovirus transmission, geographic spread, and disease severity. We find that multiple alphaviruses, including chikungunya (CHIKV), Ross River (RRV), and o'nyong 'nyong (ONNV) ... ...

    Abstract The magnitude and duration of vertebrate viremia is a critical determinant of arbovirus transmission, geographic spread, and disease severity. We find that multiple alphaviruses, including chikungunya (CHIKV), Ross River (RRV), and o'nyong 'nyong (ONNV) viruses, are cleared from the circulation of mice by liver Kupffer cells, impeding viral dissemination. Clearance from the circulation was independent of natural antibodies or complement factor C3, and instead relied on scavenger receptor SR-A6 (MARCO). Remarkably, lysine to arginine substitutions at distinct residues within the E2 glycoproteins of CHIKV and ONNV (E2 K200R) as well as RRV (E2 K251R) allowed for escape from clearance and enhanced viremia and dissemination. Mutational analysis revealed that viral clearance from the circulation is strictly dependent on the presence of lysine at these positions. These findings reveal a previously unrecognized innate immune pathway that controls alphavirus viremia and dissemination in vertebrate hosts, ultimately influencing disease severity and likely transmission efficiency.
    Mesh-Begriff(e) Alphavirus Infections/immunology ; Animals ; Chikungunya virus/immunology ; Disease Models, Animal ; Kupffer Cells/immunology ; Lysine/genetics ; Lysine/metabolism ; Mice ; Mutation, Missense ; O'nyong-nyong Virus/immunology ; Receptors, Immunologic/metabolism ; Viral Envelope Proteins/genetics ; Viral Envelope Proteins/immunology
    Chemische Substanzen Marco protein, mouse ; Receptors, Immunologic ; Viral Envelope Proteins ; Lysine (K3Z4F929H6)
    Sprache Englisch
    Erscheinungsdatum 2019-10-09
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.49163
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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