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  1. Article ; Online: Drought Stress in Brassica napus: Effects, Tolerance Mechanisms, and Management Strategies

    Batool, Maria / El-Badri, Ali Mahmoud / Hassan, Muhammad Umair / Haiyun, Yang / Chunyun, Wang / Zhenkun, Yan / Jie, Kuai / Wang, Bo / Zhou, Guangsheng

    J Plant Growth Regul. 2023 Jan., v. 42, no. 1 p.21-45

    2023  

    Abstract: Drought poses serious threats to global crop production and its intensity is continuously soaring due to global warming. Brassica napus L. is an essential oilseed crop with an important place in global edible oil production. Drought-induced yield ... ...

    Abstract Drought poses serious threats to global crop production and its intensity is continuously soaring due to global warming. Brassica napus L. is an essential oilseed crop with an important place in global edible oil production. Drought-induced yield reduction is a big problem that needs to be addressed by knowing the targeted pathways and processes. Drought stress adversely affects germination, seedling establishment, photosynthetic efficiency, mineral uptake, shoot elongation, seed development, yield and quality in rapeseed. Plants attain various physiological and molecular protective approaches for tolerance under drought stress. The currently existing agronomic, breeding and biotechnological approaches can increase the adaptability provision of a conducive environment to Brassica plants facing drought stress. In the present review, we addressed the possible cross-talk among various responses of rapeseed under drought stress and discussed the potential management strategies for regulating the drought tolerance-related mechanisms. To date, various novel approaches have been tested to minimize the adverse effects of environmental stresses in B. napus. Despite the main improvements, there is still a big room for improvement in the drought tolerance of rapeseed cultivars. Thus, future research mainly using biotechnological and molecular approaches should be carried out to develop genetically engineered rapeseed plants with enhanced drought tolerance.
    Keywords Brassica napus ; cooking fats and oils ; crop production ; cultivars ; drought ; drought tolerance ; germination ; oilseed crops ; photosynthesis ; plant establishment ; plant growth ; rapeseed ; seed development ; water stress
    Language English
    Dates of publication 2023-01
    Size p. 21-45.
    Publishing place Springer US
    Document type Article ; Online
    Note Review
    ZDB-ID 586787-3
    ISSN 1435-8107 ; 0721-7595
    ISSN (online) 1435-8107
    ISSN 0721-7595
    DOI 10.1007/s00344-021-10542-9
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Cloning and functional characterization of PjCAO gene involved in chlorophyll b biosynthesis in Pseudosasa japonica cv. Akebonosuji

    Jiang, Keyi / Haiyun Yang / Mingbing Zhou / Wei Fang

    Trees. 2016 Aug., v. 30, no. 4

    2016  

    Abstract: KEY MESSAGE : Not only the first systematic characterization of CAO gene in bamboo species, but also the first attempt to study the relationship between CAO gene expression and bamboo leaf color variation. Chlorophyllide a oxygenase (CAO) converts ... ...

    Abstract KEY MESSAGE : Not only the first systematic characterization of CAO gene in bamboo species, but also the first attempt to study the relationship between CAO gene expression and bamboo leaf color variation. Chlorophyllide a oxygenase (CAO) converts chlorophyllide (Chlide) a to Chlide b and hence plays an important role in chlorophyll (Chl) b biosynthesis. In this study, a cDNA of a CAO homologue designated PjCAO was isolated from Pseudosasa japonica cv. Akebonosuji that is a cultivar of high ornamental value in landscape due to its unique green-white striped leaf phenotype. The full-length cDNA of PjCAO was 2070 bp long with a 1626 bp open reading frame that encoded 541 amino acids. Multiple sequence alignment for amino acid showed that the putative PjCAO protein shared a high sequence similarity with CAO homologues from other plant species and consisted of four conserved parts, an amino-terminal transit peptide and three individual domains, namely, A, B, and C domain. Further, PjCAO was overexpressed in Arabidopsis thaliana; and the Chl b contents of these PjCAO-overexpressed plants were much higher than that of wild-type plants, thereby indicating its important role in Chl b synthesis. Reverse transcription real time quantitative polymerase chain reaction revealed that PjCAO was ubiquitously expressed in all the 14 tissue samples collected from P. japonica cv. Akebonosuji. Specifically, it was expressed at higher levels in 12 leaf samples than in culms and roots. Moreover, the expression of PjCAO kept increasing during the development of white, striped, and green leaf samples, thus indicating its potential role in leaf development. In addition, the expression levels of PjCAO in leaf samples within almost the same developmental stages fell into two distinct classes: high expression levels in green, and almost green with some white stripes samples; and low expression levels in white, and almost white with some green stripes samples. Collectively, these expression data suggest that PjCAO may be involved in the leaf color variation for P. japonica cv. Akebonosuji.
    Keywords amino acids ; Arabidopsis thaliana ; bamboos ; biosynthesis ; chlorophyll ; color ; complementary DNA ; culms ; cultivars ; developmental stages ; gene expression ; genes ; landscapes ; leaf development ; leaves ; open reading frames ; ornamental value ; phenotype ; Pseudosasa ; quantitative polymerase chain reaction ; reverse transcription ; roots ; sequence alignment ; sequence homology ; transcription (genetics)
    Language English
    Dates of publication 2016-08
    Size p. 1303-1314.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 90595-1
    ISSN 1432-2285 ; 0931-1890
    ISSN (online) 1432-2285
    ISSN 0931-1890
    DOI 10.1007/s00468-016-1367-8
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Ultrasound-Guided versus Fluoroscopy-Guided Deep Cervical Plexus Block for the Treatment of Cervicogenic Headache

    Qing Wan / Haiyun Yang / Xiao Li / Caina Lin / Songjian Ke / Shaoling Wu / Chao Ma

    BioMed Research International, Vol

    2017  Volume 2017

    Abstract: Objective. The aim of this study was to compare the efficacy of ultrasound-guided deep cervical plexus block with fluoroscopy-guided deep cervical plexus block for patients with cervicogenic headache (CeH). Methods. A total of 56 patients with CeH were ... ...

    Abstract Objective. The aim of this study was to compare the efficacy of ultrasound-guided deep cervical plexus block with fluoroscopy-guided deep cervical plexus block for patients with cervicogenic headache (CeH). Methods. A total of 56 patients with CeH were recruited and randomly assigned to either the ultrasound-guided (US) or the fluoroscopy-guided (FL) injection group. A mixture of 2–4 mL 1% lidocaine and 7 mg betamethasone was injected along C2 and/or C3 transverse process. The measurement of pain was evaluated by patients’ ratings of a 10-point numerical pain scale (NPS) before and 2 wks, 12 wks, and 24 wks after treatments. Results. The blocking procedures were well tolerated. The pain intensity, as measured by NPS, significantly decreased at 2 wks after injection treatment in both US and FL groups, respectively, compared with that of baseline (P < 0.05). The blocking procedures had continued, and comparable pain relieving effects appeared at 12 wks and 24 wks after treatment in both US and FL groups. There were no significant differences observed in the NPS before and 2 wks, 12 wks, and 24 wks after treatment between US and FL groups. Conclusions. The US-guided approach showed similar satisfactory effect as the FL-guided block. Ultrasonography can be an alternative method for its convenience and efficacy in deep cervical plexus block for CeH patients without radiation exposure.
    Keywords Medicine ; R
    Subject code 616
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Gene co-expression network analysis identifies BRCC3 as a key regulator in osteogenic differentiation of osteoblasts through a β-catenin signaling dependent pathway

    Lixiong Cai / Zhiqian Huo / Haiyun Yang / Fengchun He / Zhenglin Cao / Feng Wu / Lianjun Liu / Bingyin Sun

    Iranian Journal of Basic Medical Sciences, Vol 22, Iss 2, Pp 173-

    2019  Volume 178

    Abstract: Objective(s): The prognosis of osteoporosis is very poor, and it is very important to identify a biomarker for prevention of osteoporosis. In this study, we aimed to identify candidate markers in osteoporosis and to investigate the role of candidate ... ...

    Abstract Objective(s): The prognosis of osteoporosis is very poor, and it is very important to identify a biomarker for prevention of osteoporosis. In this study, we aimed to identify candidate markers in osteoporosis and to investigate the role of candidate markers in osteogenic differentiation. Materials and Methods: Using Weighted Gene Co-Expression Network analysis, we identified three hub genes might associate with osteoporosis. The mRNA expression of hub genes in osteoblasts from osteoporosis patients or healthy donor was detected by qRT-PCR. Using siRNA and overexpression, we investigated the role of hub gene BRCC3 in osteogenic differentiation by alkaline phosphatase staining and Alizarin red staining. Moreover, the role of β-catenin signaling in the osteogenic differentiation was detected by using β-catenin signaling inhibitor XAV939.Results: We identified three hub genes that might associate with osteoporosis including BRCC3, UBE2N, and UBE2K. UBE2N mRNA and UBE2K mRNA were not changed in osteoblasts isolated from osteoporosis patients, compared with healthy donors, whereas BRCC3 mRNA was significantly increased. Depletion of BRCC3 promoted the activation of alkaline phosphatase and formation of calcified nodules in osteoblasts isolated from osteoporosis patients and up-regulated β-catenin expression. XAV939 reversed the BRCC3 siRNA-induced osteogenic differentiation. Additionally, inhibited osteogenic differentiation was also observed after BACC3 overexpression, and this was accompanied by decreased β-catenin expression.Conclusion: BRCC3 is an important regulator for osteogenic differentiation of osteoblasts through β-catenin signaling, and it might be a promising target for osteoporosis treatment.
    Keywords BRCC3 ; β-catenin ; Osteoblasts ; Osteogenic differentiation ; Osteoporosis ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2019-02-01T00:00:00Z
    Publisher Mashhad University of Medical Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Breast contrast-enhanced ultrasound

    Xiaoyun Xiao / Bing Ou / Haiyun Yang / Huan Wu / Baoming Luo

    PLoS ONE, Vol 9, Iss 8, p e

    is a scoring system feasible? A preliminary study in China.

    2014  Volume 105517

    Abstract: Although many studies about breast contrast-enhanced ultrasound had been conducted, clear diagnostic criteria for evaluating enhancement patterns are still lacking. This study aims to identify significant indicators for breast contrast-enhanced ... ...

    Abstract Although many studies about breast contrast-enhanced ultrasound had been conducted, clear diagnostic criteria for evaluating enhancement patterns are still lacking. This study aims to identify significant indicators for breast contrast-enhanced ultrasound and to establish an initial scoring system.Totally 839 patients were included in the study. This study was divided into two parts. 364 patients were included in part 1 while 475 in part 2. Conventional ultrasound and contrast-enhanced ultrasound were used to examine each lesion. Only the cases in part 2 were also examined by elastography. In part 1, Logistic regression analysis was performed to predict significant variables. A 5-point scoring system was developed based on the results. In part 2, the scoring system was used to evaluate all the breast lesions. To evaluate the diagnostic efficacy of the new scoring system, it was compared with the system established for elastography and conventional ultrasound (BI-RADS).Three independent variables, namely, lesion scope, margin, and shape were selected in the final step of the logistic regression analysis in part 1. In part 2, the area under the ROC (receiver operating characteristic) curve for the contrast-enhanced scoring system was 0.912. The difference in the diagnostic capabilities of the contrast-enhanced scoring system and elastography was not statistically significant (P = 0.17). The difference in the diagnostic capabilities of the contrast-enhanced scoring system and BI-RADS was statistically significant (P<0.001).The contrast-enhanced patterns of benign and malignant breast tumors are different. The application of a 5-point scoring system for contrast-enhanced ultrasound is clinically promising.
    Keywords Medicine ; R ; Science ; Q
    Subject code 616
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Sequencing and phylogenetic analysis of the chloroplast genome of Pseudosasa japonica f. Akebonosuji

    Zhou, Mingbing / Haiyun Yang / Kaili Wang / Miaomiao An / Minmin Cheng / Wei Fang / Xiangwan Xia

    Biochemical systematics and ecology. 2016 Dec., v. 69

    2016  

    Abstract: Pseudosasa japonica f. Akebonosuji H. Okamura is a bamboo species with variable leaf colors, including albino, green, and green-white stripes. To determine whether variation in leaf color is due to mutations in the chloroplast genome, we sequenced the ... ...

    Abstract Pseudosasa japonica f. Akebonosuji H. Okamura is a bamboo species with variable leaf colors, including albino, green, and green-white stripes. To determine whether variation in leaf color is due to mutations in the chloroplast genome, we sequenced the chloroplast genomes of green and albino leaves of P. japonica f. Akebonosuji. The results indicated that the chloroplast genome included 86 protein-coding genes, seven ribosomal RNA genes, and 31 tRNA genes. The similarity of chloroplast genomes for the two leaf types was 99.98%, with variation between genes encoding for trnfM and trnT. We observed that the relative expression patterns of trnfM and trnT were reversed in green and albino leaves. Whether the differential expression of trnfM and trnT is involved in leaf color variation among P. japonica f. Akebonosuji remains unclear.With many bamboo chloroplast genomes available, we aligned the chloroplast genomes of 28 bamboo species, including P. japonica f. Akebonosuji, to analyze polymorphisms. This comparison revealed that noncoding regions possessed more nucleotide polymorphisms than coding regions. Chloroplast genomes and the nuclear gene “granule-bound starch synthase I” (GBSSI) of 28 bamboo species were used to construct evolutionary trees. Both evolutionary trees indicated that P. japonica f. Akebonosuji was clustered into Subtrib. Arundinariinae.
    Keywords albino ; bamboos ; chloroplast genome ; color ; gene expression regulation ; genes ; leaves ; mutation ; phylogeny ; Pseudosasa ; ribosomal RNA ; sequence analysis ; starch synthase ; transfer RNA
    Language English
    Dates of publication 2016-12
    Size p. 41-50.
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 0305-1978
    DOI 10.1016/j.bse.2016.08.002
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Identification of genes involved in color variation of bamboo culms by suppression subtractive hybridization

    Xia, Xiangwan / Fang Wei / Haiyun Yang / Mingbing Zhou / Renyi Gui / Ying Fu

    Plant physiology and biochemistry. 2015 Dec., v. 97

    2015  

    Abstract: Phyllostachys vivax cv. aureocaulis is a widely planted ornamental bamboo with evergreen leaves. This plant's culm exhibits a golden-yellow background color marked randomly with narrow and broad green stripes but is occasionally light green with yellow ... ...

    Abstract Phyllostachys vivax cv. aureocaulis is a widely planted ornamental bamboo with evergreen leaves. This plant's culm exhibits a golden-yellow background color marked randomly with narrow and broad green stripes but is occasionally light green with yellow stripes. In this study, we attempt to identify the molecular mechanism underlying the color variation in striped culms. We found that neither stroma nor grana lamellas were observed in plastids in yellow tissue cells, while complete chloroplasts were observed in green tissue. In addition, chlorophyll a and b were mainly distributed in ground tissue under the epiderm and in the cells surrounding the bundle sheath in the green portion of internodes. The amount of chlorophyll contained in cross-sections of the green portion of culms is significantly higher than in the yellow portion. However, carotenoid was nearly undetectable in both samples. In addition, we found that the expression levels of 7 ESTs, including PvESTs-F641 (JZ893845), PvESTs-F681 (JZ893885) and PvESTs-F798 (JZ894002), were significantly higher in green samples than that in yellow samples, while PvESTs-R200 (JZ894906), PvESTs-R541 (JZ895247), PvESTs-R333 (JZ895039) and PvESTs-R266 (JZ894972) were found at a higher level in yellow samples. These ESTs are thought to play a role in this color variation in plants. Our current results indicate that insufficient photosynthetic membrane proteins and lipids in yellow tissue could lead to chloroplast dysfunction and could result in the yellow appearance on certain P. vivax cv. aureocaulis culms.
    Keywords bamboos ; carotenoids ; chlorophyll ; color ; culms ; expressed sequence tags ; genes ; grana ; green light ; internodes ; leaves ; lipids ; membrane proteins ; photosynthesis ; Phyllostachys ; planting ; suppression subtractive hybridization
    Language English
    Dates of publication 2015-12
    Size p. 156-164.
    Publishing place Elsevier Masson SAS
    Document type Article
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2015.10.004
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Detachable string magnetically controlled capsule endoscopy for detecting high-risk varices in compensated advanced chronic liver disease (CHESS1801)

    Shuai Wang / Yifei Huang / Weiling Hu / Hua Mao / Mark Edward McAlindon / Yanna Liu / Li Yang / Chunqing Zhang / Ming Xu / Chaohui He / Tong Dang / Bin Wu / Dong Ji / Liting Zhang / Xiaorong Mao / Lei Zhang / Chuan Liu / Dan Xu / Yasong Li /
    Guoan Li / Juqiang Han / Fangfang Lv / Xiao Liang / Shaoqin Jin / Shaoheng Zhang / Foong Way David Tai / Qing Xu / Changqing Yang / Guangchuan Wang / Lifen Wang / Bo Li / Haiyun Yang / Ping Xie / Lulin Deng / Limei Ren / Zhiheng Chang / Xing Wang / Shan Wang / Xiaoqin Gao / Junfeng Li / Longdong Zhu / Fangzhao Wang / Lingen Zhang / Guo Zhang / Xi Jiang / Jun Pan / Wenbo Meng / Xun Li / Jinlin Hou / Xavier Dray

    The Lancet Regional Health. Western Pacific, Vol 6, Iss , Pp 100072- (2021)

    A prospective multicenter study

    2021  

    Abstract: Background: Gastroesophageal varices is a serious complication of compensated advanced chronic liver disease (cACLD). Primary prophylaxis to reduce the risk of variceal hemorrhage is recommended if high-risk varices (HRV) are detected. We performed this ... ...

    Abstract Background: Gastroesophageal varices is a serious complication of compensated advanced chronic liver disease (cACLD). Primary prophylaxis to reduce the risk of variceal hemorrhage is recommended if high-risk varices (HRV) are detected. We performed this study to compare the accuracy, patients’ satisfaction and safety of detection of HRV by detachable string magnetically controlled capsule endoscopy (DS-MCCE) with esophagogastroduodenoscopy (EGD) as the reference. Methods: We prospectively recruited participants with cACLD from 12 university hospitals (11 in China and one in the United Kingdom) between November 2018 and December 2019 (ClinicalTrials.gov, NCT03749954). All participants underwent DS-MCCE, followed by EGD within a week in a blinded fashion. Following endoscopy, and on the same day, participants were asked to fill in a satisfaction questionnaire regarding their experience. Findings: A total of 105 eligible participants were enrolled. With EGD as the reference standard, the concordance index, sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio of DS-MCCE in diagnosis of HRV were 0•90 (95% confidence interval [CI]: 0•83–0•95), 92% (95% CI: 78–98%), 88% (95% CI: 78–95%), 80% (95% CI: 70–92%), 95% (95% CI: 90–100%), 7•91 (95% CI: 4•10–15•30), and 0•09 (95% CI: 0•03–0•30), respectively. The kappa score of 0•78 (95% CI: 0•65–0•90) suggested substantial agreement between DS-MCCE and EGD. Moreover, in participants undergoing EGD without sedation, the satisfaction of DS-MCCE was significantly better than that of EGD (p < 0•0001, d = 1•15 [95%CI: 0•88–1•42]). All participants confirmed the excretion of the capsule, and no adverse events occurred. Interpretation: DS-MCCE is an accurate alternative to EGD for detecting HRV in cACLD, which is safe and associated with better satisfaction. Funding: A full list of funding can be found in the Funding Support section.
    Keywords Clinical trial ; Prospective ; Portal hypertension ; Gastroesophageal varices ; Esophagogastroduodenoscopy ; Public aspects of medicine ; RA1-1270
    Subject code 610
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Three-column reconstruction through single posterior approach for the treatment of unstable thoracolumbar fracture.

    Haiyun, Yang / Rui, Gu / Shucai, Deng / Zhanhua, Jia / Xiaolin, Zhang / Xin, Liu / Xue, Wang / Gongyi, Lv / Jiankun, Liu

    Spine

    2010  Volume 35, Issue 8, Page(s) E295–302

    Abstract: Study design: A prospective study was performed.: Objective: To assess a technique, three-column reconstruction through single posterior (TRSP) approach, in treatment of highly unstable thoracolumbar fracture.: Summary of background data: The goal ...

    Abstract Study design: A prospective study was performed.
    Objective: To assess a technique, three-column reconstruction through single posterior (TRSP) approach, in treatment of highly unstable thoracolumbar fracture.
    Summary of background data: The goal of surgical intervention for treatment of unstable thoracolumbar fractures is to decompress the neural elements, restore vertebral body height, correct angular deformity, and stabilize the columns of the spine. Operative approaches remain disputed. Common opinions include short-segment posterior fixation, multiple-segment posterior fixation, stand-alone anterior approach fixation, and combined anterior-posterior approach. Each technique has its advantages and disadvantages.
    Methods: A consecutive series of 37 thoracolumbar fractures with load sharing scores > or =6 were managed with TRSP between May 2004 and September 2006. All patients were observed up for a minimum of 2 years. Demographic data, neurologic status, segmental kyphosis, segmental height, the fracture severity score, Visual Analogue Scale, and treatment-related complications were evaluated.
    Results: The mean operative time was 157 minutes (range, 120-240). The mean blood loss was 1086 mL (range, 700-3100). Averagely Frankel score improved from 3.46 to 4.46; kyphotic angulation improved from 25.75 degrees before surgery to 4.49 degrees at last follow-up, and the loss of segmental height improved from a mean of 35.22% before surgery to 7.01% at the time of the last follow-up. The mean preoperative Visual Analogue Scale score was 7.92, and most patients had no complain of pain at last follow-up. No patient experienced worsening of neurologic deficits and other severe complications at last follow-up. One patient developed titanium mess cage subsidence, but revision was not necessary.
    Conclusion: The technique of TRSP approach is safe, effective, and offers some advantages over the classic posterior, anterior, combined anterior-posterior approach for some specific highly unstable thoracolumbar fractures.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Back Pain/etiology ; Back Pain/surgery ; Decompression, Surgical/instrumentation ; Decompression, Surgical/methods ; Disability Evaluation ; Female ; Humans ; Internal Fixators ; Kyphosis/etiology ; Kyphosis/surgery ; Lumbar Vertebrae/diagnostic imaging ; Lumbar Vertebrae/pathology ; Lumbar Vertebrae/surgery ; Male ; Middle Aged ; Outcome Assessment (Health Care) ; Postoperative Complications/epidemiology ; Prospective Studies ; Prosthesis Implantation/instrumentation ; Prosthesis Implantation/methods ; Radiography ; Reconstructive Surgical Procedures/instrumentation ; Reconstructive Surgical Procedures/methods ; Severity of Illness Index ; Spinal Fractures/diagnostic imaging ; Spinal Fractures/pathology ; Spinal Fractures/surgery ; Spinal Fusion/instrumentation ; Spinal Fusion/methods ; Thoracic Vertebrae/diagnostic imaging ; Thoracic Vertebrae/pathology ; Thoracic Vertebrae/surgery ; Treatment Outcome ; Young Adult
    Language English
    Publishing date 2010-04-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 752024-4
    ISSN 1528-1159 ; 0362-2436
    ISSN (online) 1528-1159
    ISSN 0362-2436
    DOI 10.1097/BRS.0b013e3181c392b9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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