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Artikel ; Online: Establishment of an intragastric surgical model using C57BL/6 mice to study the vaccine efficacy of OMV-based immunogens against Helicobacter pylori.

Das, Sanjib / Halder, Prolay / Banerjee, Soumalya / Mukhopadhyay, Asish Kumar / Dutta, Shanta / Koley, Hemanta

2024

Abstract: Chronic gastritis is one of the major symptoms of gastro-duodenal disorders typically induced by Helicobacter pylori (H. pylori). To date, no suitable model is available to study pathophysiology and therapeutic measures accurately. Here, we have ... ...

Abstract	Chronic gastritis is one of the major symptoms of gastro-duodenal disorders typically induced by Helicobacter pylori (H. pylori). To date, no suitable model is available to study pathophysiology and therapeutic measures accurately. Here, we have presented a successful surgical infection model of H. pylori-induced gastritis in C57BL/6 mice that resembles features similar to human infection. The proposed model does not require any preparatory treatment other than surgical intervention. C57BL/6 mice were injected with wild-type SS1 (Sydney strain 1, reference strain) directly into the stomach. Seven days post infection, infected animals showed alterations in cytokine responses along with inflammatory cell infiltration in the lamina propria, depicting a prominent inflammatory response due to infection. To understand the immunogenicity and protective efficacy, the mice were immunized with outer membrane vesicles (OMVs) isolated from an indigenous strain with putative virulence factors of H. pylori [A61C (1), cag+/vacA s1m1]. In contrast to the non-immunized cohort, the OMV-immunized cohort showed a gradual increase in serum immunoglobulin(s) levels on the 35th day after the first immunization. This conferred protective immunity against subsequent challenge with the reference strain (SS1). Direct inoculation of H. pylori into the stomach influenced infection in a short time and, more importantly, in a dose-dependent manner, indicating the usefulness of the developed model for pathophysiology, therapeutic and prophylactic studies.
Sprache	Englisch
Erscheinungsdatum	2024-04-11
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2632264-X
ISSN	2046-6390 ; 2046-6390
ISSN (online)	2046-6390
ISSN	2046-6390
DOI	10.1242/bio.060282
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Intranasal immunization of mice with chimera of Salmonella Typhi protein elicits protective intestinal immunity.

Chakraborty, Suparna / Dutta, Pujarini / Pal, Ananda / Chakraborty, Swarnali / Banik, George / Halder, Prolay / Gope, Animesh / Miyoshi, Shin-Ichi / Das, Santasabuj

NPJ vaccines

2024 Band 9, Heft 1, Seite(n) 24

Abstract: Development of safe, highly effective and affordable enteric fever vaccines is a global health priority. Live, oral typhoid vaccines induce strong mucosal immunity and long-term protection, but safety remains a concern. In contrast, efficacy wears off ... ...

Abstract	Development of safe, highly effective and affordable enteric fever vaccines is a global health priority. Live, oral typhoid vaccines induce strong mucosal immunity and long-term protection, but safety remains a concern. In contrast, efficacy wears off rapidly for injectable, polysaccharide-based vaccines, which elicit poor mucosal response. We previously reported Salmonella Typhi outer membrane protein, T2544 as a potential candidate for bivalent (S. Typhi and S. Paratyphi A) vaccine development. Here, we show that intranasal immunization with a subunit vaccine (chimera of T2544 and cholera toxin B subunit) induced strong systemic and intestinal mucosal immunity and protection from S. Typhi challenge in a mouse model. CTB-T2544 augmented gut-homing receptor expression on lymphocytes that produced Th1 and Th17 cytokines, secretory IgA in stool that inhibited bacterial motility and epithelial attachment, antibody recall response and affinity maturation with increased number of follicular helper T cells and CD4+ central and effector memory cells.
Sprache	Englisch
Erscheinungsdatum	2024-02-06
Erscheinungsland	England
Dokumenttyp	Journal Article
ISSN	2059-0105
ISSN (online)	2059-0105
DOI	10.1038/s41541-024-00812-4
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Stable Recombinant Invasion Plasmid Antigen C (IpaC)-Based Single Dose Nanovaccine for Shigellosis.

Baruah, Namrata / Halder, Prolay / Koley, Hemanta / Katti, Dhirendra S

Molecular pharmaceutics

2022 Band 19, Heft 11, Seite(n) 3884–3893

Abstract: Shigellosis, caused by the ... ...

Abstract	Shigellosis, caused by the bacteria
Mesh-Begriff(e)	Mice ; Animals ; Dysentery, Bacillary/prevention & control ; Antigens, Bacterial/genetics ; Antibodies, Bacterial ; Plasmids/genetics ; Mice, Inbred BALB C ; Diarrhea
Chemische Substanzen	Antigens, Bacterial ; Antibodies, Bacterial
Sprache	Englisch
Erscheinungsdatum	2022-09-19
Erscheinungsland	United States
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2138405-8
ISSN	1543-8392 ; 1543-8384
ISSN (online)	1543-8392
ISSN	1543-8384
DOI	10.1021/acs.molpharmaceut.2c00378
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Rotavirus non-structural protein 4 usurps host cellular RIPK1-RIPK3 complex to induce MLKL-dependent necroptotic cell death.

Chandra, Pritam / Patra, Upayan / Mukhopadhyay, Urbi / Mukherjee, Arpita / Halder, Prolay / Koley, Hemanta / Chawla-Sarkar, Mamta

Biochimica et biophysica acta. Molecular cell research

2024 Band 1871, Heft 5, Seite(n) 119745

Abstract: The dynamic interface between invading viral pathogens and programmed cell death (PCD) of the host is a finely regulated process. Host cellular demise at the end of the viral life cycle ensures the release of progeny virions to initiate new infection ... ...

Abstract	The dynamic interface between invading viral pathogens and programmed cell death (PCD) of the host is a finely regulated process. Host cellular demise at the end of the viral life cycle ensures the release of progeny virions to initiate new infection cycles. Rotavirus (RV), a diarrheagenic virus with double-stranded RNA genome, has been reported to trigger different types of PCD such as apoptosis and pyroptosis in a highly regulated way to successfully disseminate progeny virions. Recently our lab also showed that induction of MLKL-driven programmed necroptosis by RV. However, the host cellular machinery involved in RV-induced necroptosis and the upstream viral trigger responsible for it remained unaddressed. In the present study, the signalling upstream of MLKL-driven necroptosis has been delineated where the involvement of Receptor interacting serine/threonine kinase 3 (RIPK3) and 1 (RIPK1) from the host side and RV non-structural protein 4 (NSP4) as the viral trigger for necroptosis has been shown. Interestingly, RV-NSP4 was found to be an integral component of the necrosome complex by interacting with RIPK1, thereby bypassing the requirement of RIPK1 kinase activity. Subsequently, NSP4-driven elevated cytosolic Ca
Mesh-Begriff(e)	Receptor-Interacting Protein Serine-Threonine Kinases/metabolism ; Receptor-Interacting Protein Serine-Threonine Kinases/genetics ; Viral Nonstructural Proteins/metabolism ; Viral Nonstructural Proteins/genetics ; Humans ; Protein Kinases/metabolism ; Protein Kinases/genetics ; Necroptosis ; Rotavirus/metabolism ; Animals ; Host-Pathogen Interactions ; Toxins, Biological/metabolism
Chemische Substanzen	Receptor-Interacting Protein Serine-Threonine Kinases (EC 2.7.11.1) ; Viral Nonstructural Proteins ; Protein Kinases (EC 2.7.-) ; MLKL protein, human (EC 2.7.-) ; RIPK3 protein, human (EC 2.7.11.1) ; RIPK1 protein, human (EC 2.7.11.1) ; NS28 protein, rotavirus ; Toxins, Biological
Sprache	Englisch
Erscheinungsdatum	2024-05-06
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbamcr.2024.119745
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Facile synthesis of multi-faceted, biomimetic and cross-protective nanoparticle-based vaccines for drug-resistant Shigella: a flexible platform technology.

Baruah, Namrata / Ahamad, Nadim / Halder, Prolay / Koley, Hemanta / Katti, Dhirendra S

Journal of nanobiotechnology

2023 Band 21, Heft 1, Seite(n) 34

Abstract: Background: No commercial vaccines are available against drug-resistant Shigella due to serotype-specific/narrow-range of protection. Nanoparticle-based biomimetic vaccines involving stable, conserved, immunogenic proteins fabricated using facile ... ...

Abstract	Background: No commercial vaccines are available against drug-resistant Shigella due to serotype-specific/narrow-range of protection. Nanoparticle-based biomimetic vaccines involving stable, conserved, immunogenic proteins fabricated using facile chemistries can help formulate a translatable cross-protective Shigella vaccine. Such systems can also negate cold-chain transportation/storage thus overcoming challenges prevalent in various settings. Methods: We explored facile development of biomimetic poly (lactide-co-glycolide)/PLGA 50:50 based nanovaccines (NVs), encapsulating conserved stabilized antigen(s)/immunostimulant of S. dysenteriae 1 origin surface-modified using simple chemistries. All encapsulants (IpaC/IpaB/LPS) and nanoparticles (NPs)-bare and modified (NV), were thoroughly characterized. Effect of IpaC on cellular uptake of NPs was assessed in-vitro. Immunogenicity of the NVs was assessed in-vivo in BALB/c mice by intranasal immunization. Cross-protective efficacy was assessed by intraperitoneally challenging the immunized groups with a high dose of heterologous S. flexneri 2a and observing for visible diarrhea, weight loss and survival. Passive-protective ability of the simplest NV was assessed in the 5-day old progeny of vaccinated mice. Results: All the antigens and immunostimulant to be encapsulated were successfully purified and found to be stable both before and after encapsulation into NPs. The ~ 300 nm sized NPs with a zeta potential of ~ - 25 mV released ~ 60% antigen by 14th day suggesting an appropriate delivery kinetics. The NPs could be successfully surface-modified with IpaC and/or CpG DNA. In vitro experiments revealed that the presence of IpaC can significantly increase cellular uptake of NPs. All NVs were found to be cytocompatible and highly immunogenic. Antibodies in sera of NV-immunized mice could recognize heterologous Shigella. Immunized sera also showed high antibody and cytokine response. The immunized groups were protected from diarrhea and weight loss with ~ 70-80% survival upon heterologous Shigella challenge. The simplest NV showed ~ 88% survival in neonates. Conclusions: Facile formulation of biomimetic NVs can result in significant cross-protection. Further, passive protection in neonates suggest that parental immunization could protect infants, the most vulnerable group in context of Shigella infection. Non-invasive route of vaccination can also lead to greater patient compliance making it amenable for mass-immunization. Overall, our work contributes towards a yet to be reported platform technology for facile development of cross-protective Shigella vaccines.
Mesh-Begriff(e)	Animals ; Mice ; Pharmaceutical Preparations ; Biomimetics ; Shigella ; Adjuvants, Immunologic ; Shigella Vaccines/genetics ; Nanoparticles ; Antibodies, Bacterial ; Mice, Inbred BALB C
Chemische Substanzen	Pharmaceutical Preparations ; Adjuvants, Immunologic ; Shigella Vaccines ; Antibodies, Bacterial
Sprache	Englisch
Erscheinungsdatum	2023-01-29
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2100022-0
ISSN	1477-3155 ; 1477-3155
ISSN (online)	1477-3155
ISSN	1477-3155
DOI	10.1186/s12951-023-01780-y
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Controlling the bacterial load of Salmonella Typhi in an experimental mouse model by a lytic Salmonella phage STWB21: a phage therapy approach.

Mondal, Payel / Halder, Prolay / Mallick, Bani / Bhaumik, Subhadip / Koley, Hemanta / Dutta, Shanta / Dutta, Moumita

BMC microbiology

2023 Band 23, Heft 1, Seite(n) 324

Abstract: Background: Salmonella enterica serotype Typhi is one of the major pathogens causing typhoid fever and a public health burden worldwide. Recently, the increasing number of multidrug-resistant strains of Salmonella spp. has made this utmost necessary to ... ...

Abstract	Background: Salmonella enterica serotype Typhi is one of the major pathogens causing typhoid fever and a public health burden worldwide. Recently, the increasing number of multidrug-resistant strains of Salmonella spp. has made this utmost necessary to consider bacteriophages as a potential alternative to antibiotics for S. Typhi infection treatment. Salmonella phage STWB21, isolated from environmental water, has earlier been reported to be effective as a safe biocontrol agent by our group. In this study, we evaluated the efficacy of phage STWB21 in reducing the burden of salmonellosis in a mammalian host by inhibiting Salmonella Typhi invasion into the liver and spleen tissue. Results: Phage treatment significantly improved the survival percentage of infected mice. This study also demonstrated that oral administration of phage treatment could be beneficial in both preventive and therapeutic treatment of salmonellosis caused by S. Typhi. Altogether the result showed that the phage treatment could control tissue inflammation in mice before and after Salmonella infection. Conclusions: To the best of our knowledge, this is the first report of phage therapy in a mouse model against a clinically isolated Salmonella Typhi strain that includes direct visualization of histopathology and ultrathin section microscopy images from the liver and spleen sections.
Mesh-Begriff(e)	Animals ; Mice ; Salmonella typhi ; Phage Therapy ; Bacterial Load ; Typhoid Fever/therapy ; Typhoid Fever/microbiology ; Salmonella Infections/therapy ; Bacteriophages ; Salmonella Phages ; Mammals
Sprache	Englisch
Erscheinungsdatum	2023-11-03
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2041505-9
ISSN	1471-2180 ; 1471-2180
ISSN (online)	1471-2180
ISSN	1471-2180
DOI	10.1186/s12866-023-03040-3
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Bacterial ghost cell based bivalent candidate vaccine against Salmonella Typhi and Salmonella Paratyphi A: A prophylactic study in BALB/c mice.

Halder, Prolay / Maiti, Suhrid / Banerjee, Soumalya / Das, Sanjib / Dutta, Moumita / Dutta, Shanta / Koley, Hemanta

Vaccine

2023 Band 41, Heft 41, Seite(n) 5994–6007

Abstract: Typhoid and emerging paratyphoid fever are a severe enteric disease worldwide with high morbidity and mortality. Licensed typhoid vaccines are in the market, but no paratyphoid vaccine is currently available. In the present study we developed a bivalent ... ...

Abstract	Typhoid and emerging paratyphoid fever are a severe enteric disease worldwide with high morbidity and mortality. Licensed typhoid vaccines are in the market, but no paratyphoid vaccine is currently available. In the present study we developed a bivalent vaccine against Salmonella Typhi and Paratyphi A using a bacterial ghost platform. Bacterial ghost cells (BGs) are bacteria-derived cell membranes without cytoplasmic contents that retain their cellular morphology, including all cell surface features. Furthermore, BGs have inherent adjuvant properties that promote an enhanced humoral and cellular immune reaction to the target antigen. Sodium hydroxide was used to prepare ghost cells of Salmonella Typhi and Paratyphi A. The bacterial ghost cells were characterised using electron microscopy. Then BALB/c mice were immunized three times (0
Mesh-Begriff(e)	Mice ; Animals ; Salmonella typhi ; Salmonella paratyphi A ; Mice, Inbred BALB C ; Typhoid Fever/prevention & control ; Typhoid-Paratyphoid Vaccines
Chemische Substanzen	Typhoid-Paratyphoid Vaccines
Sprache	Englisch
Erscheinungsdatum	2023-08-23
Erscheinungsland	Netherlands
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2023.08.049
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Suppression of classical nuclear import pathway by importazole and ivermectin inhibits rotavirus replication.

Sarkar, Rakesh / Banerjee, Shreya / Halder, Prolay / Koley, Hemanta / Komoto, Satoshi / Chawla-Sarkar, Mamta

The Journal of antimicrobial chemotherapy

2022 Band 77, Heft 12, Seite(n) 3443–3455

Abstract: Background: Rotavirus is the foremost cause of acute gastroenteritis among infants in resource-poor countries, causing severe morbidity and mortality. The currently available rotavirus vaccines are effective in reducing severity of the disease but not ... ...

Abstract	Background: Rotavirus is the foremost cause of acute gastroenteritis among infants in resource-poor countries, causing severe morbidity and mortality. The currently available rotavirus vaccines are effective in reducing severity of the disease but not the infection rates, thus antivirals as an adjunct therapy are needed to reduce the morbidity in children. Viruses rely on host cellular machinery for nearly every step of the replication cycle. Therefore, targeting host factors that are indispensable for virus replication could be a promising strategy. Objectives: To assess the therapeutic potential of ivermectin and importazole against rotaviruses. Methods: Antirotaviral activity of importazole and ivermectin was measured against various rotavirus strains (RV-SA11, RV-Wa, RV-A5-13, RV-EW) in vitro and in vivo by quantifying viral protein expression by western blot, analysing viroplasm formation by confocal microscopy, and measuring virus yield by plaque assay. Results: Importin-β1 and Ran were found to be induced during rotavirus infection. Knocking down importin-β1 severely impaired rotavirus replication, suggesting a critical role for importin-β1 in the rotavirus life cycle. In vitro studies revealed that treatment of ivermectin and importazole resulted in reduced synthesis of viral proteins, diminished production of infectious virus particles, and decrease in viroplasm-positive cells. Mechanistic study proved that both drugs perform antirotavirus activity by inhibiting the function of importin-β1. In vivo investigations in mice also confirmed the antirotavirus potential of importazole and ivermectin at non-toxic doses. Treatments of rotavirus-infected mice with either drug resulted in diminished shedding of viral particles in the stool sample, reduced expression of viral protein in the small intestine and restoration of damaged intestinal villi comapared to untreated infected mice. Conclusions: The study highlights the potential of importazole and ivermectin as antirotavirus therapeutics.
Mesh-Begriff(e)	Animals ; Mice ; Active Transport, Cell Nucleus ; Ivermectin/pharmacology ; Karyopherins/metabolism ; Rotavirus/drug effects ; Rotavirus/physiology ; Viral Proteins ; Virus Replication ; Rotavirus Infections/drug therapy
Chemische Substanzen	importazole ; Ivermectin (70288-86-7) ; Karyopherins ; Viral Proteins
Sprache	Englisch
Erscheinungsdatum	2022-10-10
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dkac339
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: A tetravalent Shigella outer membrane vesicles based candidate vaccine offered cross-protection against all the serogroups of Shigella in adult mice.

Bhaumik, Ushasi / Halder, Prolay / Howlader, Debaki Ranjan / Banerjee, Soumalya / Maiti, Suhrid / Dutta, Shanta / Koley, Hemanta

Microbes and infection

2023 Band 25, Heft 5, Seite(n) 105100

Abstract: In today's world and mostly in low and middle income countries, Shigella flexneri and Shigella sonnei remains the major causative agent of clinical bacillary dysentery. Based on contemporary epidemiology, a tetravalent Outer Membrane Vesicle (OMVs) based ...

Abstract	In today's world and mostly in low and middle income countries, Shigella flexneri and Shigella sonnei remains the major causative agent of clinical bacillary dysentery. Based on contemporary epidemiology, a tetravalent Outer Membrane Vesicle (OMVs) based immunogen was formulated using the most commonly circulating Shigella strains, namely, S. flexneri 2a, S. flexneri 3a, S. flexneri 6 and S. sonnei I, in a 1:1:1:1 ratio. Adult BALB/c mice were orally immunized in a prime-boost-boost manner. Tetravalent Shigella OMVs immunogen induced significant and persistent serum and mucosal antibodies against OMVs, Outer Membrane Proteins (OMPs) and lipopolysaccharides (LPS). Tetravalent OMVs also primed cell mediated immune response effectively. Protective efficacy against six heterologous Shigella strains was checked in an intra-peritoneal mouse model. Immunized mice survived lethal infection better than the non-immunized mice cohort with fewer replicating bacteria isolated from their gut. This study establishes the possibilities of tetravalent OMVs immunogen to become a potent vaccine candidate against human shigellosis, overcoming the limitations of sero-specific cross-protection of Shigella species.
Mesh-Begriff(e)	Animals ; Adult ; Humans ; Mice ; Shigella Vaccines ; Serogroup ; Shigella ; Dysentery, Bacillary/prevention & control ; Dysentery, Bacillary/microbiology ; Shigella flexneri ; Vaccines ; Antibodies, Bacterial
Chemische Substanzen	Shigella Vaccines ; Vaccines ; Antibodies, Bacterial
Sprache	Englisch
Erscheinungsdatum	2023-01-22
Erscheinungsland	France
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1465093-9
ISSN	1769-714X ; 1286-4579
ISSN (online)	1769-714X
ISSN	1286-4579
DOI	10.1016/j.micinf.2023.105100
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Bivalent non-typhoidal Salmonella outer membrane vesicles immunized mice sera confer passive protection against gastroenteritis in a suckling mice model

Maiti, Suhrid / Howlader, Debaki Ranjan / Halder, Prolay / Bhaumik, Ushasi / Dutta, Moumita / Dutta, Shanta / Koley, Hemanta

Vaccine. 2021 Jan. 08, v. 39, no. 2

2021

Abstract: Invasive non-typhoidal Salmonella (iNTS) serovars, especially Salmonella Typhimurium (ST) and Salmonella Enteritidis (SE), cause gastroenteritis worldwide. Due to the emergence of multi-drug resistance in iNTS, a broad-spectrum vaccine is urgently needed ...

Abstract	Invasive non-typhoidal Salmonella (iNTS) serovars, especially Salmonella Typhimurium (ST) and Salmonella Enteritidis (SE), cause gastroenteritis worldwide. Due to the emergence of multi-drug resistance in iNTS, a broad-spectrum vaccine is urgently needed for the prevention of iNTS infection. Currently, there is no effective licensed vaccine against iNTS available in the market. We have formulated an outer membrane vesicles (OMVs) based bivalent immunogen as a vaccine candidate to generate broad-spectrum protective immunity against both recently circulating prevalent ST and SE. We have isolated OMVs from ST and SE and formulated the immunogen by mixing both OMVs (1:1 ratio). Three doses of bivalent immunogen significantly induced humoral immune responses against lipopolysaccharides (LPSs) and outer membrane proteins (OMPs) as well as a cell-mediated immune response in adult mice. We also observed that proteins of OMVs act as an adjuvant for generation of high levels of anti-LPS antibodies through T cell activation. We then characterized the one-day old suckling mice model for both ST and SE mediated gastroenteritis and used the model for a passive protection study. In the passive protection study, we found the passive transfer of bivalent OMVs immunized sera significantly reduced ST and SE mediated colonization and gastroenteritis symptoms in the colon of suckling mice compared to non-immunized sera recipients. The overall study demonstrated that OMVs based bivalent vaccine could generate broad-spectrum immunity against prevalent iNTS mediated gastroenteritis. This study also established the suckling mice model as a suitable animal model for vaccine study against iNTS mediated gastroenteritis.
Schlagwörter	Salmonella Enteritidis ; Salmonella Typhimurium ; T-lymphocytes ; adjuvants ; adults ; animal models ; antigens ; cell-mediated immunity ; colon ; gastroenteritis ; lipopolysaccharides ; markets ; multiple drug resistance ; serotypes ; vaccines
Sprache	Englisch
Erscheinungsverlauf	2021-0108
Umfang	p. 380-393.
Erscheinungsort	Elsevier Ltd
Dokumenttyp	Artikel
Anmerkung	NAL-AP-2-clean
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2020.11.040
Datenquelle	NAL Katalog (AGRICOLA)

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Zusatzmaterialien

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Zusatzmaterialien

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Zusatzmaterialien

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Verfügbar in ZB MED Köln/Königswinter

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Zusatzmaterialien

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Verfügbar in ZB MED Köln/Königswinter

Über subito bestellen