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Article ; Online: Seeking and embracing change.

Hamasaki, Maho

2018 Volume 20, Issue 9, Page(s) 1002

MeSH term(s)	Asian Continental Ancestry Group/psychology ; Biomedical Research/history ; Career Choice ; Career Mobility ; Cell Biology/history ; Cultural Characteristics ; Gender Identity ; History, 20th Century ; History, 21st Century ; Humans ; Male ; Research Personnel/history ; Research Personnel/psychology ; Women, Working/history ; Women, Working/psychology
Language	English
Publishing date	2018-08-27
Publishing country	England
Document type	Autobiography ; Historical Article ; Journal Article ; Portrait
ZDB-ID	1474722-4
ISSN	1476-4679 ; 1465-7392
ISSN (online)	1476-4679
ISSN	1465-7392
DOI	10.1038/s41556-018-0180-6
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Zs.A 5169: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 12: Show issues

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Article ; Online: Monitoring and assessment of lysosomal membrane damage in cultured cells using the high-content imager.

Tabata, Keisuke / Saeki, Marika / Yoshimori, Tamotsu / Hamasaki, Maho

STAR protocols

2023 Volume 4, Issue 2, Page(s) 102236

Abstract: Autophagy is an intracellular self-degradation process in which part of the cytoplasm, aggregates, or damaged organelles are degraded in lysosomes. Lysophagy is a specific form of selective autophagy responsible for clearing damaged lysosomes. Here, we ... ...

Abstract	Autophagy is an intracellular self-degradation process in which part of the cytoplasm, aggregates, or damaged organelles are degraded in lysosomes. Lysophagy is a specific form of selective autophagy responsible for clearing damaged lysosomes. Here, we present a protocol for inducing lysosomal damage in cultured cells and assessing lysosomal damage using a high-content imager and software program. We describe steps for induction of lysosomal damage, image acquisition with spinning disk confocal microscopy, and image analysis using Pathfinder. We then detail data analysis of the clearance of damaged lysosomes. For complete details on the use and execution of this protocol, please refer to Teranishi et al. (2022).
Language	English
Publishing date	2023-04-18
Publishing country	United States
Document type	Journal Article
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2023.102236
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Article ; Online: How cells recognize and remove the perforated lysosome.

Tabata, Keisuke / Saeki, Marika / Yoshimori, Tamotsu / Hamasaki, Maho

Autophagy

2022 Volume 19, Issue 6, Page(s) 1869–1871

Abstract: Macroautophagy (hereafter autophagy) is a highly conserved intracellular degradation system to maintain cellular homeostasis by degrading cellular components such as misfolded proteins, nonfunctional organelles, pathogens, and cytosol. Conversely, ... ...

Abstract	Macroautophagy (hereafter autophagy) is a highly conserved intracellular degradation system to maintain cellular homeostasis by degrading cellular components such as misfolded proteins, nonfunctional organelles, pathogens, and cytosol. Conversely, selective autophagy targets and degrades specific cargo, such as organelles, bacteria,
MeSH term(s)	Autophagy ; Lysosomes/metabolism ; Macroautophagy ; Ubiquitination ; Organelles/metabolism ; Proteins/metabolism
Chemical Substances	Proteins
Language	English
Publishing date	2022-11-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2454135-7
ISSN	1554-8635 ; 1554-8627
ISSN (online)	1554-8635
ISSN	1554-8627
DOI	10.1080/15548627.2022.2138686
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Zs.A 6741: Show issues

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Article ; Online: Autophagy and kidney aging.

Minami, Satoshi / Yamamoto, Takeshi / Yamamoto-Imoto, Hitomi / Isaka, Yoshitaka / Hamasaki, Maho

Progress in biophysics and molecular biology

2023 Volume 179, Page(s) 10–15

Abstract: Autophagy is a highly conserved intracellular degradation system in eukaryotes that maintains cellular and tissue homeostasis. Upon autophagy induction, cytoplasmic components are engulfed by a double-membrane organelle called the autophagosome that ... ...

Abstract	Autophagy is a highly conserved intracellular degradation system in eukaryotes that maintains cellular and tissue homeostasis. Upon autophagy induction, cytoplasmic components are engulfed by a double-membrane organelle called the autophagosome that fuses with a lysosome to degrade its contents. In recent years, it has become clear that autophagy becomes dysregulated with aging, which leads to age-related diseases. Kidney function is particularly prone to age-related decline, and aging is the most significant risk factor for chronic kidney disease. This review first discuss the relationship between autophagy and kidney aging. Second, we describe how age-related dysregulation of autophagy occurs. Finally, we discuss the potential of autophagy-targeting drugs to ameliorate human kidney aging and the approaches necessary to discover such agents.
MeSH term(s)	Humans ; Autophagy ; Kidney ; Autophagosomes/metabolism ; Aging ; Organelles
Language	English
Publishing date	2023-02-26
Publishing country	England
Document type	Review ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209302-9
ISSN	1873-1732 ; 0079-6107
ISSN (online)	1873-1732
ISSN	0079-6107
DOI	10.1016/j.pbiomolbio.2023.02.005
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Uc I Zs.281: Show issues

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Article ; Online: The mechanisms and roles of selective autophagy in mammals.

Vargas, Jose Norberto S / Hamasaki, Maho / Kawabata, Tsuyoshi / Youle, Richard J / Yoshimori, Tamotsu

Nature reviews. Molecular cell biology

2022 Volume 24, Issue 3, Page(s) 167–185

Abstract: Autophagy is a process that targets various intracellular elements for degradation. Autophagy can be non-selective - associated with the indiscriminate engulfment of cytosolic components - occurring in response to nutrient starvation and is commonly ... ...

Abstract	Autophagy is a process that targets various intracellular elements for degradation. Autophagy can be non-selective - associated with the indiscriminate engulfment of cytosolic components - occurring in response to nutrient starvation and is commonly referred to as bulk autophagy. By contrast, selective autophagy degrades specific targets, such as damaged organelles (mitophagy, lysophagy, ER-phagy, ribophagy), aggregated proteins (aggrephagy) or invading bacteria (xenophagy), thereby being importantly involved in cellular quality control. Hence, not surprisingly, aberrant selective autophagy has been associated with various human pathologies, prominently including neurodegeneration and infection. In recent years, considerable progress has been made in understanding mechanisms governing selective cargo engulfment in mammals, including the identification of ubiquitin-dependent selective autophagy receptors such as p62, NBR1, OPTN and NDP52, which can bind cargo and ubiquitin simultaneously to initiate pathways leading to autophagy initiation and membrane recruitment. This progress opens the prospects for enhancing selective autophagy pathways to boost cellular quality control capabilities and alleviate pathology.
MeSH term(s)	Animals ; Humans ; Macroautophagy ; Proteins/metabolism ; Autophagy ; Ubiquitin/metabolism ; Mammals/metabolism
Chemical Substances	Proteins ; Ubiquitin
Language	English
Publishing date	2022-10-27
Publishing country	England
Document type	Journal Article ; Review ; Research Support, N.I.H., Intramural
ZDB-ID	2031313-5
ISSN	1471-0080 ; 1471-0072
ISSN (online)	1471-0080
ISSN	1471-0072
DOI	10.1038/s41580-022-00542-2
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Zs.A 5446: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 26: Show issues

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Article ; Online: Selective-plane-activation structured illumination microscopy.

Temma, Kenta / Oketani, Ryosuke / Kubo, Toshiki / Bando, Kazuki / Maeda, Shunsuke / Sugiura, Kazunori / Matsuda, Tomoki / Heintzmann, Rainer / Kaminishi, Tatsuya / Fukuda, Koki / Hamasaki, Maho / Nagai, Takeharu / Fujita, Katsumasa

Nature methods

2024

Abstract: The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize ...

Abstract	The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.
Language	English
Publishing date	2024-04-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2169522-2
ISSN	1548-7105 ; 1548-7091
ISSN (online)	1548-7105
ISSN	1548-7091
DOI	10.1038/s41592-024-02236-3
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Zs.A 6022: Show issues

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Article ; Online: Three-Color Simultaneous Live Imaging of Autophagy-Related Structures.

Ueda, Hiroyuki / Kunitaki, Ouin / Hamasaki, Maho

Methods in molecular biology (Clifton, N.J.)

2018 Volume 1880, Page(s) 223–230

Abstract: Simultaneous live cell imaging of multiple proteins helps to analyze mobility and interactions among proteins over time. Since autophagosomes depend on other organelles for their formation, it is necessary to observe this process with multiple ... ...

Abstract	Simultaneous live cell imaging of multiple proteins helps to analyze mobility and interactions among proteins over time. Since autophagosomes depend on other organelles for their formation, it is necessary to observe this process with multiple fluorsphores to mark multiple organelles and the autophagosomes. To do so, we set up three cameras on one microscope to be able to acquire three colors at the same time. Here we describe the setup using a Yokogawa spinning disk confocal microscope (CSU-W1) with Andor TuCam system attaching 3 × Zyla 4.2 CMOS cameras (Andor) and detail the method for acquiring live images.
MeSH term(s)	Animals ; Autophagosomes/ultrastructure ; Autophagy ; COS Cells ; Cell Line ; Cell Survival ; Cercopithecus aethiops ; Equipment Design ; Humans ; Image Processing, Computer-Assisted/instrumentation ; Image Processing, Computer-Assisted/methods ; Microscopy, Confocal/instrumentation ; Microscopy, Confocal/methods ; Microscopy, Fluorescence/instrumentation ; Microscopy, Fluorescence/methods ; Optical Imaging/instrumentation ; Optical Imaging/methods ; Software
Language	English
Publishing date	2018-12-20
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-8873-0_14
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Article ; Online: Identification of CUL4A-DDB1-WDFY1 as an E3 ubiquitin ligase complex involved in initiation of lysophagy.

Teranishi, Hirofumi / Tabata, Keisuke / Saeki, Marika / Umemoto, Tetsuo / Hatta, Tomohisa / Otomo, Takanobu / Yamamoto, Kentaro / Natsume, Toru / Yoshimori, Tamotsu / Hamasaki, Maho

Cell reports

2022 Volume 40, Issue 11, Page(s) 111349

Abstract: Macroautophagy is a bulk degradation system in which double membrane-bound structures called autophagosomes to deliver cytosolic materials to lysosomes. Autophagy promotes cellular homeostasis by selectively recognizing and sequestering specific targets, ...

Abstract	Macroautophagy is a bulk degradation system in which double membrane-bound structures called autophagosomes to deliver cytosolic materials to lysosomes. Autophagy promotes cellular homeostasis by selectively recognizing and sequestering specific targets, such as damaged organelles, protein aggregates, and invading bacteria, termed selective autophagy. We previously reported a type of selective autophagy, lysophagy, which helps clear damaged lysosomes. Damaged lysosomes become ubiquitinated and recruit autophagic machinery. Proteomic studies using transfection reagent-coated beads and further evaluations reveal that a CUL4A-DDB1-WDFY1 E3 ubiquitin ligase complex is essential to initiate lysophagy and clear damaged lysosomes. Moreover, we show that LAMP2 is ubiquitinated by the CUL4A E3 ligase complex as a substrate on damaged lysosomes. These results reveal how cells selectively tag damaged lysosomes to initiate autophagy for the clearance of lysosomes.
MeSH term(s)	Lysosomes/metabolism ; Macroautophagy ; Proteomics ; Ubiquitin/metabolism ; Ubiquitin-Protein Ligases/metabolism
Chemical Substances	Ubiquitin ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
Language	English
Publishing date	2022-09-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2022.111349
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Article ; Online: Loss of RUBCN/rubicon in adipocytes mediates the upregulation of autophagy to promote the fasting response.

Yamamuro, Tadashi / Nakamura, Shuhei / Yanagawa, Kyosuke / Tokumura, Ayaka / Kawabata, Tsuyoshi / Fukuhara, Atsunori / Teranishi, Hirofumi / Hamasaki, Maho / Shimomura, Iichiro / Yoshimori, Tamotsu

Autophagy

2022 Volume 18, Issue 11, Page(s) 2686–2696

Abstract: Upon fasting, adipocytes release their lipids that accumulate in the liver, thus promoting hepatic steatosis and ketone body production. However, the mechanisms underlying this process are not fully understood. In this study, we found that fasting caused ...

Abstract	Upon fasting, adipocytes release their lipids that accumulate in the liver, thus promoting hepatic steatosis and ketone body production. However, the mechanisms underlying this process are not fully understood. In this study, we found that fasting caused a substantial decrease in the adipose levels of RUBCN/rubicon, a negative regulator of macroautophagy/autophagy, along with an increase in autophagy. Adipose-specific
MeSH term(s)	Mice ; Animals ; Autophagy/genetics ; Fasting ; Up-Regulation/genetics ; Adipocytes/metabolism ; Adipogenesis ; Mice, Knockout ; Fatty Liver/metabolism ; Carrier Proteins/metabolism ; PPAR gamma/genetics ; Intracellular Signaling Peptides and Proteins/metabolism
Chemical Substances	Carrier Proteins ; PPAR gamma ; Intracellular Signaling Peptides and Proteins
Language	English
Publishing date	2022-03-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2454135-7
ISSN	1554-8635 ; 1554-8627
ISSN (online)	1554-8635
ISSN	1554-8627
DOI	10.1080/15548627.2022.2047341
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Zs.A 6741: Show issues

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Article ; Online: Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging.

Ogura, Monami / Kaminishi, Tatsuya / Shima, Takayuki / Torigata, Miku / Bekku, Nao / Tabata, Keisuke / Minami, Satoshi / Nishino, Kohei / Nezu, Akiko / Hamasaki, Maho / Kosako, Hidetaka / Yoshimori, Tamotsu / Nakamura, Shuhei

EMBO reports

2023 Volume 24, Issue 12, Page(s) e57300

Abstract: Lysosomes are degradative organelles and signaling hubs that maintain cell and tissue homeostasis, and lysosomal dysfunction is implicated in aging and reduced longevity. Lysosomes are frequently damaged, but their repair mechanisms remain unclear. Here, ...

Abstract	Lysosomes are degradative organelles and signaling hubs that maintain cell and tissue homeostasis, and lysosomal dysfunction is implicated in aging and reduced longevity. Lysosomes are frequently damaged, but their repair mechanisms remain unclear. Here, we demonstrate that damaged lysosomal membranes are repaired by microautophagy (a process termed "microlysophagy") and identify key regulators of the first and last steps. We reveal the AGC kinase STK38 as a novel microlysophagy regulator. Through phosphorylation of the scaffold protein DOK1, STK38 is specifically required for the lysosomal recruitment of the AAA+ ATPase VPS4, which terminates microlysophagy by promoting the disassembly of ESCRT components. By contrast, microlysophagy initiation involves non-canonical lipidation of ATG8s, especially the GABARAP subfamily, which is required for ESCRT assembly through interaction with ALIX. Depletion of STK38 and GABARAPs accelerates DNA damage-induced cellular senescence in human cells and curtails lifespan in C. elegans, respectively. Thus, microlysophagy is regulated by STK38 and GABARAPs and could be essential for maintaining lysosomal integrity and preventing aging.
MeSH term(s)	Animals ; Humans ; Microautophagy ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Lysosomes/metabolism ; Intracellular Membranes/metabolism ; Endosomal Sorting Complexes Required for Transport/genetics ; Endosomal Sorting Complexes Required for Transport/metabolism ; Autophagy ; Microtubule-Associated Proteins/metabolism ; Apoptosis Regulatory Proteins/metabolism ; Protein Serine-Threonine Kinases/genetics ; Protein Serine-Threonine Kinases/metabolism
Chemical Substances	Endosomal Sorting Complexes Required for Transport ; GABARAP protein, human ; Microtubule-Associated Proteins ; Apoptosis Regulatory Proteins ; STK38 protein, human (EC 2.7.11.1) ; Protein Serine-Threonine Kinases (EC 2.7.11.1)
Language	English
Publishing date	2023-11-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2020896-0
ISSN	1469-3178 ; 1469-221X
ISSN (online)	1469-3178
ISSN	1469-221X
DOI	10.15252/embr.202357300
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Zs.A 5433: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 41: Show issues

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