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  1. Article ; Online: Analysis, Design and Realization of a Furnace for In Situ Wettability Experiments at High Temperatures under X-ray Microtomography.

    Fedele, Roberto / Hameed, Fareeha / Cefis, Nicola / Vergani, Gabriele

    Journal of imaging

    2021  Volume 7, Issue 11

    Abstract: In this study, we analyzed the problem of a compact furnace, to be used for in situ experiments in a cone-beam X-ray microtomography commercial system. The design process was accomplished and outlined through its main steps, until the realization of a ... ...

    Abstract In this study, we analyzed the problem of a compact furnace, to be used for in situ experiments in a cone-beam X-ray microtomography commercial system. The design process was accomplished and outlined through its main steps, until the realization of a prototype. The furnace was conceived to carry out wettability experiments at temperatures up to 700 °C and under inert atmosphere on sessile droplets of a molten metal alloy, with a few millimeters diameter, posed on a thin ceramic substrate. X-ray imaging of the molten droplet is expected to permit an accurate three-dimensional reconstruction of the droplet profile and a robust estimation of the related quantities (such as the contact angle and the surface tension) utilized for the assessment of metal-ceramic joints by brazing. The challenges faced during this project, mostly related to the constraints of the setup, and the novel solutions implemented were discussed also with the support of analytical and numerical tools, in terms of interaction of X-rays with matter, geometry and working principle, heat transfer and insulation, material selection.
    Language English
    Publishing date 2021-11-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2824270-1
    ISSN 2313-433X ; 2313-433X
    ISSN (online) 2313-433X
    ISSN 2313-433X
    DOI 10.3390/jimaging7110240
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Tissue engineered periosteum: Fabrication of a gelatin basedtrilayer composite scaffold with biomimetic properties for enhanced bone healing.

    Tariq, Sana / Shah, Saqlain A / Hameed, Fareeha / Mutahir, Zeeshan / Khalid, Hamad / Tufail, Asma / Akhtar, Hafsah / Chaudhry, Aqif Anwar / Khan, Ather Farooq

    International journal of biological macromolecules

    2024  Volume 263, Issue Pt 2, Page(s) 130371

    Abstract: The periosteum, a vascularized tissue membrane, is essential in bone regeneration following fractures and bone loss due to some other reasons, yet there exist several research gaps concerning its regeneration. These gaps encompass reduced cellular ... ...

    Abstract The periosteum, a vascularized tissue membrane, is essential in bone regeneration following fractures and bone loss due to some other reasons, yet there exist several research gaps concerning its regeneration. These gaps encompass reduced cellular proliferation and bioactivity, potential toxicity, heightened stiffness of scaffold materials, unfavorable porosity, expensive materials and procedures, and suboptimal survivability or inappropriate degradation rates of the implanted materials. This research used an interdisciplinary approach by forming a new material fabricated through electrospinning for the proposed application as a layer-by-layer tissue-engineered periosteum (TEP). TEP comprises poly(ε-caprolactone) (PCL), PCL/gelatin/magnesium-doped zinc oxide (vascular layer), and gelatin/bioactive glass/COD liver oil (osteoconductive layer). These materials were selected for their diverse properties, when integrated into the scaffold formation, successfully mimic the characteristics of native periosteum. Scanning electron microscopy (SEM) was employed to confirm the trilayer structure of the scaffold and determine the average fiber diameter. In-vitro degradation and swelling studies demonstrated a uniform degradation rate that matches the typical recovery time of periosteum. The scaffold exhibited excellent mechanical properties comparable to natural periosteum. Furthermore, the sustained release kinetics of COD liver oil were observed in the trilayer scaffold. Cell culture results indicated that the three-dimensional topography of the scaffold promoted cell growth, proliferation, and attachment, confirming its non-toxicity, biocompatibility, and bioactivity. This study suggests that the fabricated scaffold holds promise as a potential artificial periosteum for treating periostitis and bone fractures.
    MeSH term(s) Tissue Scaffolds/chemistry ; Gelatin/chemistry ; Periosteum ; Biomimetics ; Cod Liver Oil ; Polyesters/chemistry ; Tissue Engineering/methods
    Chemical Substances Gelatin (9000-70-8) ; Cod Liver Oil (8001-69-2) ; Polyesters
    Language English
    Publishing date 2024-02-27
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.130371
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
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  3. Article ; Online: Detection of MCR-1 Gene in Multiple Drug Resistant Escherichia coli and Klebsiella pneumoniae in Human Clinical Samples from Peshawar, Pakistan.

    Hameed, Fareeha / Khan, Muhammad Asif / Bilal, Hazrat / Muhammad, Hafsah / Tayyab Ur Rehman

    Combinatorial chemistry & high throughput screening

    2020  Volume 24, Issue 5, Page(s) 737–742

    Abstract: Background: The presence of plasmid mediated mcr-1 gene in multidrug resistant Gram-negative bacteria poses a serious public health concern in today's world.: Objective: The present study was aimed to detect the presence of plasmid mediated mcr-1 ... ...

    Abstract Background: The presence of plasmid mediated mcr-1 gene in multidrug resistant Gram-negative bacteria poses a serious public health concern in today's world.
    Objective: The present study was aimed to detect the presence of plasmid mediated mcr-1 encoding resistance to colistin in multiple drug resistant (MDR) E. coli and K. pneumoniae isolates.
    Methods: A total of 180 clinical isolates of E. coli (n=120) and K. pneumoniae (n=60) were isolated from different clinical specimens, i.e., urine, blood, stool and pus, from diagnostic labs of two major public sector tertiary care hospitals in Peshawar, Pakistan. MDR profile of these isolates was assessed through Kirby-Baur disc diffusion method. All isolates were screened for colistin resistance by dilution methods. Colistin resistant isolates were subjected to PCR for mcr-1 detection and confirmation was done by Sanger sequencing method.
    Results: Overall, 83.3% (100/120) E. coli and 93.3% (56/60) K. pneumoniae were detected as MDR. Colistin resistance was found in 23.3% (28/120) E. coli and 40% (24/60) K. pneumoniae isolates, whereas mcr-1 gene was detected in 10 out of 52 colistin resistant isolates, including six E. coli and four K. pneumoniae isolates. Minimum inhibitory concentrations (MICs) of colistin in these ten mcr-1 positive isolates ranged from 4μg/ml to 16μg/ml. All mcr-1 positive isolates showed 99% sequence similarity when compared with other present sequences in GenBank.
    Conclusion: Hence, our study confirms the presence of mcr-1 mediated colistin resistance in the studied area. Therefore, urgently larger scale surveillance studies are recommended to investigate prevalence of mcr-1 mediated colistin resistance and to prevent its further spread in the area.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Colistin/pharmacology ; Dose-Response Relationship, Drug ; Drug Resistance, Multiple, Bacterial/genetics ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/isolation & purification ; Escherichia coli Proteins/metabolism ; Humans ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/genetics ; Pakistan
    Chemical Substances Anti-Bacterial Agents ; Escherichia coli Proteins ; MCR-1 protein, E coli ; Colistin (Z67X93HJG1)
    Language English
    Publishing date 2020-09-14
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2064785-2
    ISSN 1875-5402 ; 1386-2073
    ISSN (online) 1875-5402
    ISSN 1386-2073
    DOI 10.2174/1386207323666200914100119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5577: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Detection of blaNDM-1 gene in ESBL producing Escherichia coli and Klebsiella pneumoniae isolated from urine samples.

    Naeem, Sohni / Bilal, Hazrat / Muhammad, Hafsah / Khan, Muhammad Asif / Hameed, Fareeha / Bahadur, Sher / Rehman, Tayyab Ur

    Journal of infection in developing countries

    2021  Volume 15, Issue 4, Page(s) 516–522

    Abstract: Introduction: Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae are the most prominent bacterial species resistant to almost all commonly used antibiotics. Carbapenem is one of the last resort drugs for treating such emerging ... ...

    Abstract Introduction: Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae are the most prominent bacterial species resistant to almost all commonly used antibiotics. Carbapenem is one of the last resort drugs for treating such emerging multidrug-resistant bacteria. This study aimed to detect carbapenem-resistant blaNDM-1 gene in ESBL producing E. coli and K. pneumoniae isolates.
    Methodology: A total of 190 E. coli and 350 K. pneumoniae isolates were screened for extended spectrumβ-lactamase (ESBL), carbapenemase and metallo β-lactamase (MBL) production via double-disk synergy test (DDST), modified Hodge test and combined-disk diffusion method. The blaNDM-1 gene was detected by PCR and confirmed via Sanger sequencing method.
    Results: Of the 540 isolates tested, 71.8% were found to be multidrug-resistant. Overall rate of ESBL-positive isolates were 57.89% E. coli and 31.42% K. pneumoniae. Among ESBL positive isolates, 49.09% E. coli and 40% K. pneumoniae were positive for carbapenemase production whereas MBL production was detected in 29% E. coli and 22% K. pneumoniae isolates. In MBL positive isolates, (37%) E. coli and (40%) K. pneumoniae isolates harboured blaNDM-1 gene. The pair-wise DNA was aligned with the NDM-1 sequence from GenBank. The alignment score was 243 and the blast nucleotide sequencing results showed 97% sequence similarity with the sequences in GenBank for the blaNDM-1 gene.
    Conclusions: The blaNDM-1 gene was found to be the most prevalent in urine samples. There is a dire need to conduct screening tests in hospitals and communities to find out the exact prevalence of the blaNDM-1 spread in our population.
    MeSH term(s) Anti-Bacterial Agents/therapeutic use ; Carbapenems/therapeutic use ; Cross-Sectional Studies ; Drug Resistance, Multiple, Bacterial/drug effects ; Drug Resistance, Multiple, Bacterial/genetics ; Escherichia coli/isolation & purification ; Humans ; Klebsiella pneumoniae/isolation & purification ; Microbial Sensitivity Tests/methods ; beta-Lactamases/urine
    Chemical Substances Anti-Bacterial Agents ; Carbapenems ; beta-Lactamases (EC 3.5.2.6) ; beta-lactamase NDM-1 (EC 3.5.2.6)
    Language English
    Publishing date 2021-04-30
    Publishing country Italy
    Document type Journal Article ; Multicenter Study
    ZDB-ID 2394024-4
    ISSN 1972-2680 ; 2036-6590
    ISSN (online) 1972-2680
    ISSN 2036-6590
    DOI 10.3855/jidc.12850
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Molecular Epidemiology of

    Bilal, Hazrat / Rehman, Tayyab Ur / Khan, Muhammad Asif / Hameed, Fareeha / Jian, Zhang Gao / Han, Jianxiong / Yang, Xingyuan

    Infection and drug resistance

    2021  Volume 14, Page(s) 1467–1479

    Abstract: Purpose: The multiple-drug resistant : Methods: In total, 545 strains of : Findings: A total of four (0.73%) colistin-resistant strains carrying alongside : Conclusion: ... ...

    Abstract Purpose: The multiple-drug resistant
    Methods: In total, 545 strains of
    Findings: A total of four (0.73%) colistin-resistant strains carrying alongside
    Conclusion: The
    Language English
    Publishing date 2021-04-16
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2494856-1
    ISSN 1178-6973
    ISSN 1178-6973
    DOI 10.2147/IDR.S302687
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Plasmid-mediated mcr-1 gene in Acinetobacter baumannii and Pseudomonas aeruginosa: first report from Pakistan.

    Hameed, Fareeha / Khan, Muhammad Asif / Muhammad, Hafsah / Sarwar, Tahir / Bilal, Hazrat / Rehman, Tayyab Ur

    Revista da Sociedade Brasileira de Medicina Tropical

    2019  Volume 52, Page(s) e20190237

    Abstract: Introduction: The increased use of colistin against infections caused by Acinetobacter baumannii and Pseudomonas aeruginosa has resulted in colistin resistance. The purpose of this study was to detect plasmid-mediated mcr-1 gene in colistin-resistant A. ...

    Abstract Introduction: The increased use of colistin against infections caused by Acinetobacter baumannii and Pseudomonas aeruginosa has resulted in colistin resistance. The purpose of this study was to detect plasmid-mediated mcr-1 gene in colistin-resistant A. baumannii and P. aeruginosa isolates.
    Methods: A total of 146 clinical isolates of A. baumannii (n = 62) and P. aeruginosa (n = 84) were collected from the four largest tertiary care hospitals in Peshawar, Pakistan. All bacterial isolates were phenotypically screened for multidrug resistance using the Kirby-Baur disc diffusion method. The minimum inhibitory concentration (MIC) of colistin in all isolates was phenotypically performed using dilution methods. mcr-1 gene was detected through polymerase chain reaction and the nucleotide sequence of amplicon was determined using Sanger sequencing.
    Results: Approximately 96.7% A. baumannii and 83.3% P. aeruginosa isolates were resistant to multiple antibiotics. Colistin resistance was found in 9.6% (6/62) of A. baumannii and 11.9% (10/84) of P. aeruginosa isolates. Among 16 colistin resistant isolates, the mcr-1 gene was detected in one A. baumannii (1.61% of total isolates; 16.6% of colistin resistant isolates) and one P. aeruginosa strain (1.19% of total isolates; 10% of colistin resistant isolates). Nucleotide BLAST showed 98-99% sequence similarity to sequences of the mcr-1 gene in GenBank.
    Conclusions: Our study reports, for the first time, the emergence of plasmid-mediated mcr-1-encoded colistin resistance in multidrug resistant strains of A. baumannii and P. aeruginosa. Further large scales studies are recommended to investigate the prevalence of this mode of resistance in these highly pathogenic bacteria.
    MeSH term(s) Acinetobacter Infections/microbiology ; Acinetobacter baumannii/drug effects ; Acinetobacter baumannii/genetics ; Bacterial Proteins/genetics ; Drug Resistance, Bacterial ; Humans ; Microbial Sensitivity Tests ; Pakistan ; Plasmids/genetics ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics
    Chemical Substances Bacterial Proteins
    Language English
    Publishing date 2019-09-05
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 1038126-0
    ISSN 1678-9849 ; 0037-8682
    ISSN (online) 1678-9849
    ISSN 0037-8682
    DOI 10.1590/0037-8682-0237-2019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2726: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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