Article: A sensitive LC-MS assay using derivatization with boron trifluoride to quantify curcuminoids in biological samples
Analytical biochemistry. 2020 May 01, v. 596
2020
Abstract: A procedure is described to measure curcumin (C), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC), tetrahydrocurcumim (TC) and their glucuronidated metabolites (CG, DMCG, and BDMCG) in plasma, brain, liver and tumor samples. The procedure involves ... ...
| Abstract | A procedure is described to measure curcumin (C), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC), tetrahydrocurcumim (TC) and their glucuronidated metabolites (CG, DMCG, and BDMCG) in plasma, brain, liver and tumor samples. The procedure involves converting the analytes to their boron difluoride derivatives and analyzing them by combined liquid chromatography coupled to an ion trap mass spectrometer operating in the negative ion MSn scan mode. The method has superb limits of detection of 0.01 nM for all curcuminoids and 0.5 nM for TC and the glucuroniated metabolites, and several representative chromatograms of biological samples containing these analytes are provided. In addition, the pharmacokinetic profile of these compounds in one human who daily consumed an over-the-counter curcuminoid product shows the peak and changes in circulating concentrations achieved by this mode of administration. |
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| Keywords | blood plasma ; boron ; brain ; chemical species ; curcumin ; derivatization ; detection limit ; humans ; liquid chromatography ; liver ; mass spectrometry ; metabolites ; neoplasms ; pharmacokinetics ; spectrometers |
| Language | English |
| Dates of publication | 2020-0501 |
| Publishing place | Elsevier Inc. |
| Document type | Article |
| ZDB-ID | 1110-1 |
| ISSN | 1096-0309 ; 0003-2697 |
| ISSN (online) | 1096-0309 |
| ISSN | 0003-2697 |
| DOI | 10.1016/j.ab.2020.113636 |
| Database | NAL-Catalogue (AGRICOLA) |
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