Article ; Online: Hsa_circ_0004872 alleviates meningioma progression by sponging miR-190a-3p/PTEN signaling.
2024 Volume 24, Issue 1, Page(s) 345
Abstract: Background: Meningioma, the most prevalent intracranial tumor, possesses a significant propensity for malignant transformation. Circular RNAs (circ-RNAs), a class of non-coding RNAs, have emerged as crucial players in tumorigenesis. This study explores ... ...
| Abstract | Background: Meningioma, the most prevalent intracranial tumor, possesses a significant propensity for malignant transformation. Circular RNAs (circ-RNAs), a class of non-coding RNAs, have emerged as crucial players in tumorigenesis. This study explores the functional relevance of hsa_circ_0004872, a specific circ-RNA, in the context of meningioma. Methods: Molecular structure and stability of hsa_circ_0004872 were elucidated through PCR identification. Meningioma cell proliferation and apoptosis were assessed using the CCK-8 assay and flow cytometry, respectively. Gene and protein expression were analyzed via qRT-PCR and western blot. Molecular interactions were confirmed through dual-luciferase reporter gene and RIP assays. Results: Hsa_circ_0004872, derived from exons 2 to 4 of the host gene MAPK1, demonstrated enhanced stability compared to its host MAPK1. Clinical data described that hsa_circ_0004872 was reduced in meningioma tissues and cell lines, and negatively correlated to poor survival rate of meningioma patients. Overexpression of hsa_circ_0004872 exhibited inhibitory effects on cell proliferation and promotion of apoptosis in vitro. Subsequent investigations unveiled a direct interaction between hsa_circ_0004872 and miR-190a-3p, leading to the activation of the PI3K/AKT signaling pathway through targeting PTEN. Notably, miR-190a-3p silence accelerated the apoptosis and proliferation inhibition of meningioma cells by inactivating PTEN/PI3K/AKT signaling, while miR-190a-3p overexpression showed an opposite effect, which greatly reversed the anti-tumor effects of hsa_circ_0004872 overexpression. Conclusion: In summary, our findings highlighted the intricate role of hsa_circ_0004872 in meningioma, shedding light on the regulatory mechanisms involving circ-RNAs in tumor progression. This positions hsa_circ_0004872 as a potential key regulatory factor in meningioma with implications for future therapeutic interventions. |
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| MeSH term(s) | Humans ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation/genetics ; Cell Transformation, Neoplastic ; Gene Expression Regulation, Neoplastic ; Meningeal Neoplasms/genetics ; Meningioma/genetics ; MicroRNAs/genetics ; Phosphatidylinositol 3-Kinases/genetics ; Proto-Oncogene Proteins c-akt/genetics ; PTEN Phosphohydrolase/genetics ; Signal Transduction/genetics |
| Chemical Substances | MicroRNAs ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; PTEN Phosphohydrolase (EC 3.1.3.67) ; PTEN protein, human (EC 3.1.3.67) ; MIRN190 microRNA, human |
| Language | English |
| Publishing date | 2024-03-18 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2041352-X |
| ISSN | 1471-2407 ; 1471-2407 |
| ISSN (online) | 1471-2407 |
| ISSN | 1471-2407 |
| DOI | 10.1186/s12885-024-12084-1 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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