Article ; Online: Pure platelet-rich plasma promotes semaphorin-3A expression: a novel insight to ameliorate intervertebral disk degeneration in vitro.
Journal of orthopaedic surgery and research
2023 Volume 18, Issue 1, Page(s) 789
Abstract: Introduction: Intervertebral disk degeneration (IVDD) can be effectively treated using platelet-rich plasma (PRP). While the exact process is fully understood, it is believed that using pure PRP (P-PRP) without leukocytes is a better option for ... ...
| Abstract | Introduction: Intervertebral disk degeneration (IVDD) can be effectively treated using platelet-rich plasma (PRP). While the exact process is fully understood, it is believed that using pure PRP (P-PRP) without leukocytes is a better option for preventing IVDD. Semaphorin-3A (Sema3A), an inhibitor of angiogenesis and innervation, is essential for preserving IVDD's homeostasis. Whether PRP prevents IVDD by modifying Sema3A has yet to receive much research. This work aims to clarify how P-PRP affects Sema3A when IVDD develops in vitro. Methods: Nucleus pulposus cells (NPCs) isolated from 8-week-old male Sprague-Dawley rats were exposed to 10 ng/ml IL-1β and then treated with P-PRP or leukocyte platelet-rich plasma (L-PRP) in vitro, followed by measuring cell proliferation, apoptosis and microstructures, inflammatory gene and Sema3A expression, as well as anabolic and catabolic protein expression by immunostaining, quantitative real-time polymerase chain reaction (qPCR), western blot, and enzyme-linked immunosorbent assay (ELISA). Results: In comparison with L-PRP, P-PRP had a higher concentration of growth factors but a lower concentration of inflammatory substances. P-PRP increased the proliferation of NPCs, while IL-1 relieved the amount of apoptosis due to its intervention. Anabolic genes, aggrecan, and collagen II had higher expression levels. MMP-3 and ADAMTS-4, two catabolic or inflammatory genes, showed lower expression levels. Sema3A activity was enhanced after P-PRP injection, whereas CD31 and NF200 expression levels were suppressed. Conclusions: P-PRP enhanced the performance of NPCs in IVDD by modifying the NF-κB signaling pathway and encouraging Sema3A expression, which may offer new therapy options for IVDD. The translational potential of this article: The findings provide a new therapeutic target for the treatment of IVDD and show a novel light on the probable mechanism of PRP and the function of Sema3A in the progression of IVDD. |
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| MeSH term(s) | Animals ; Male ; Rats ; Collagen/metabolism ; Intervertebral Disc Degeneration/therapy ; Intervertebral Disc Degeneration/metabolism ; Platelet-Rich Plasma/metabolism ; Rats, Sprague-Dawley ; Semaphorin-3A/analysis ; Semaphorin-3A/metabolism |
| Chemical Substances | Collagen (9007-34-5) ; Semaphorin-3A ; Sema3a protein, rat |
| Language | English |
| Publishing date | 2023-10-20 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2252548-8 |
| ISSN | 1749-799X ; 1749-799X |
| ISSN (online) | 1749-799X |
| ISSN | 1749-799X |
| DOI | 10.1186/s13018-023-04290-7 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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