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  1. Article: MTSS1 inhibits metastatic potential and induces G2/M phase cell cycle arrest in gastric cancer.

    Liu, Ke / Jiao, Xiao-Dong / Hao, Jie-Lu / Qin, Bao-Dong / Wu, Ying / Chen, Wei / Liu, Jun / He, Xi / Zang, Yuan-Sheng

    OncoTargets and therapy

    2019  Volume 12, Page(s) 5143–5152

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2019-07-02
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2495130-4
    ISSN 1178-6930
    ISSN 1178-6930
    DOI 10.2147/OTT.S203165
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Reductive Amination Combining Dimethylation for Quantitative Analysis of Early-Stage Glycated Proteins

    Tong, Qing-He / Hao-Jie Lu / Lei Zhang / Li-Qi Xie / Tao Tao / Tian-Yang Yan

    Analytical chemistry. 2018 Feb. 21, v. 90, no. 6

    2018  

    Abstract: Due to the critical role glycation plays in many serious pathological conditions, such as diabetes, it is of great significance to discover protein glycation at an early stage for precaution and prediction of the disease. Here, a method of reductive ... ...

    Abstract Due to the critical role glycation plays in many serious pathological conditions, such as diabetes, it is of great significance to discover protein glycation at an early stage for precaution and prediction of the disease. Here, a method of reductive amination combining dimethylation (RAD) was developed for the quantification of early-stage glycated proteins. The quantitative analysis was first carried out by reducing the samples using NaBH3CN or NaBD3CN, resulting in a 1 Da mass shift and the stabilization of early-stage protein glycation. The two samples were then digested and isotopically dimethylated to achieve the mass shift of 4m + 3n (m represents the number of N-termini and Lys residues, and n represents the number of glycated sites) between light- and heavy-labeled glycated peptides for quantification. Consequently, the false positive result can be removed according to the different mass shifts of glycated peptides and non-glycated peptides. In quantification of glycated myoglobin, RAD showed good linearity (R2 > 0.99) and reproducibility (CVs ≤ 1.6%) in 2 orders of magnitude (1:10–10:1). RAD was then applied to quantify the endogenous glycated proteins in the serum of diabetic patients, revealing significant differences in the glycation level between the patients with complicated retinal detachment and those without. In conclusion, RAD is an effective method for quantifying endogenous glycated proteins.
    Keywords amination ; blood serum ; diabetes ; glycation ; myoglobin ; patients ; peptides ; prediction ; quantitative analysis
    Language English
    Dates of publication 2018-0221
    Size p. 3752-3758.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.7b03668
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article: ELISA–PLA: A novel hybrid platform for the rapid, highly sensitive and specific quantification of proteins and post-translational modifications

    Tong, Qing-He / Hao-Jie Lu / Li-Qi Xie / Tao Tao

    Biosensors & bioelectronics. 2016 June 15, v. 80

    2016  

    Abstract: Detection of low-abundance proteins and their post-translational modifications (PTMs) remains a great challenge. A conventional enzyme-linked immunosorbent assay (ELISA) is not sensitive enough to detect low-abundance PTMs and suffers from nonspecific ... ...

    Abstract Detection of low-abundance proteins and their post-translational modifications (PTMs) remains a great challenge. A conventional enzyme-linked immunosorbent assay (ELISA) is not sensitive enough to detect low-abundance PTMs and suffers from nonspecific detection. Herein, a rapid, highly sensitive and specific platform integrating ELISA with a proximity ligation assay (PLA), termed ELISA–PLA, was developed. Using ELISA–PLA, the specificity was improved by the simultaneous and proximate recognition of targets through multiple probes, and the sensitivity was significantly improved by rolling circle amplification (RCA). For GFP, the limit of detection (LOD) was decreased by two orders of magnitude compared to that of ELISA. Using site-specific phospho-antibody and pan-specific phospho-antibody, ELISA–PLA was successfully applied to quantify the phosphorylation dynamics of ERK1/2 and the overall tyrosine phosphorylation level of ERK1/2, respectively. ELISA–PLA was also used to quantify the O-GlcNAcylation of AKT, c-Fos, CREB and STAT3, which is faster and more sensitive than the conventional immunoprecipitation and western blotting (IP–WB) method. As a result, the sample consumption of ELISA–PLA was reduced 40-fold compared to IP–WB. Therefore, ELISA–PLA could be a promising platform for the rapid, sensitive and specific detection of proteins and PTMs.
    Keywords biosensors ; detection limit ; enzyme-linked immunosorbent assay ; phosphorylation ; post-translational modification ; precipitin tests ; proteins ; tyrosine ; Western blotting
    Language English
    Dates of publication 2016-0615
    Size p. 385-391.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2016.02.006
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

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  4. Article ; Online: CARM1 Methylates GAPDH to Regulate Glucose Metabolism and Is Suppressed in Liver Cancer

    Xing-Yu Zhong / Xiu-Ming Yuan / Ying-Ying Xu / Miao Yin / Wei-Wei Yan / Shao-Wu Zou / Li-Ming Wei / Hao-Jie Lu / Yi-Ping Wang / Qun-Ying Lei

    Cell Reports, Vol 24, Iss 12, Pp 3207-

    2018  Volume 3223

    Abstract: Summary: Increased aerobic glycolysis is a hallmark of cancer metabolism. How cancer cells coordinate glucose metabolism with extracellular glucose levels remains largely unknown. Here, we report that coactivator-associated arginine methyltransferase 1 ( ... ...

    Abstract Summary: Increased aerobic glycolysis is a hallmark of cancer metabolism. How cancer cells coordinate glucose metabolism with extracellular glucose levels remains largely unknown. Here, we report that coactivator-associated arginine methyltransferase 1 (CARM1 or PRMT4) signals glucose availability to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and suppresses glycolysis in liver cancer cells. CARM1 methylates GAPDH at arginine 234 (R234), inhibiting its catalytic activity. Glucose starvation leads to CARM1 upregulation, further inducing R234 hypermethylation and GAPDH inhibition. The re-expression of wild-type GAPDH, but not of its methylation-mimetic mutant, sustains glycolytic levels. CARM1 inhibition increases glycolytic flux and glycolysis. R234 methylation delays tumor cell proliferation in vitro and in vivo. Compared with normal tissues, R234 is hypomethylated in malignant clinical hepatocellular carcinoma samples. Notably, R234 methylation positively correlates with CARM1 expression in these liver cancer samples. Our findings thus reveal that CARM1-mediated GAPDH methylation is a key regulatory mechanism of glucose metabolism in liver cancer. : GAPDH is a critical enzyme in glycolysis. Zhong et al. find that CARM1 methylates GAPDH at R234 and inhibits its activity in an AMPK-dependent manner. R234 methylation inhibits glycolysis and proliferation of liver cancer cell lines. In hepatocellular carcinoma patient samples, GADPH R234 is hypomethylated, and there is a positive correlation between CARM1 levels and R234 methylation. Keywords: GAPDH, CARM1, arginine methylation, coenzyme affinity, glycolysis, AMPK, nutrient sensing, Warburg effect, metabolic reprogramming, liver cancer
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Solving signal instability to maintain the second-order advantage in the resolution and determination of multi-analytes in complex systems by modeling liquid chromatography–mass spectrometry data using alternating trilinear decomposition method assisted with piecewise direct standardization

    Gu, Hui-Wen / Hai-Long Wu / Hao-Jie Lu / Hui Xia / Li-Xia Xie / Peng-Yuan Yang / Ru-Qin Yu / Shan-Shan Li / Xiao-Li Yin / Ya-Juan Liu

    Journal of chromatography. 2015 Aug. 14, v. 1407

    2015  

    Abstract: The application of calibration transfer methods has been successful in combination with near-infrared spectroscopy or other tools for prediction of chemical composition. One of the developed methods that can provide accurate performances is the piecewise ...

    Abstract The application of calibration transfer methods has been successful in combination with near-infrared spectroscopy or other tools for prediction of chemical composition. One of the developed methods that can provide accurate performances is the piecewise direct standardization (PDS) method, which in this paper is firstly applied to transfer from one day to another the second-order calibration model based on alternating trilinear decomposition (ATLD) method built for the interference-free resolution and determination of multi-analytes in complex systems by liquid chromatography–mass spectrometry (LC–MS) in full scan mode. This is an example of LC–MS analysis in which interferences have been found, making necessary the use of second-order calibration because of its capacity for modeling this phenomenon, which implies analytes of interest can be resolved and quantified even in the presence of overlapped peaks and unknown interferences. Once the second-order calibration model based on ATLD method was built, the calibration transfer was conducted to compensate for the signal instability of LC–MS instrument over time. This allows one to reduce the volume of the heavy works for complete recalibration which is necessary for later accurate determinations. The root-mean-square error of prediction (RMSEP) and average recovery were used to evaluate the performances of the proposed strategy. Results showed that the number of calibration samples used on the real LC–MS data was reduced by using the PDS method from 11 to 3 while producing comparable RMSEP values and recovery values that were statistically the same (F-test, 95% confidence level) to those obtained with 11 calibration samples. This methodology is in accordance with the highly recommended green analytical chemistry principles, since it can reduce the experimental efforts and cost with regard to the use of a new calibration model built in modified conditions.
    Keywords analytical chemistry ; calibration ; chemical composition ; liquid chromatography ; mass spectrometry ; models ; near-infrared spectroscopy ; prediction
    Language English
    Dates of publication 2015-0814
    Size p. 157-168.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 218139-3
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    DOI 10.1016/j.chroma.2015.06.049
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 813: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  6. Article ; Online: VHL deficiency augments anthracycline sensitivity of clear cell renal cell carcinomas by down-regulating ALDH2

    Yao-Hui Gao / Zhao-Xia Wu / Li-Qi Xie / Cai-Xia Li / Yu-Qin Mao / Yan-Tao Duan / Bing Han / San-Feng Han / Yun Yu / Hao-Jie Lu / Peng-Yuan Yang / Tian-Rui Xu / Jing-Lin Xia / Guo-Qiang Chen / Li-Shun Wang

    Nature Communications, Vol 8, Iss 1, Pp 1-

    2017  Volume 14

    Abstract: The VHL tumour suppressor gene is lost in approximately 70% of clear cell renal cell carcinoma (ccRCC). In this study, the authors demonstrate that VHL loss in these tumours augments anthracyclines chemotherapy by down-regulation of ALDH2. ...

    Abstract The VHL tumour suppressor gene is lost in approximately 70% of clear cell renal cell carcinoma (ccRCC). In this study, the authors demonstrate that VHL loss in these tumours augments anthracyclines chemotherapy by down-regulation of ALDH2.
    Keywords Science ; Q
    Language English
    Publishing date 2017-06-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Multi-targeted interference-free determination of ten β-blockers in human urine and plasma samples by alternating trilinear decomposition algorithm-assisted liquid chromatography–mass spectrometry in full scan mode: Comparison with multiple reaction monitoring

    Gu, Hui-Wen / Hai-Long Wu / Hao-Jie Lu / Hui Xia / Peng-Yuan Yang / Ru-Qin Yu / Shu-Rong Zhang / Xiao-Dong Sun / Xiao-Li Yin / Ya-Juan Liu / Yi-Feng Jin / Yong Li

    Analytica chimica acta. 2014 Oct. 27, v. 848

    2014  

    Abstract: β-blockers are the first-line therapeutic agents for treating cardiovascular diseases and also a class of prohibited substances in athletic competitions. In this work, a smart strategy that combines three-way liquid chromatography–mass spectrometry ( ... ...

    Abstract β-blockers are the first-line therapeutic agents for treating cardiovascular diseases and also a class of prohibited substances in athletic competitions. In this work, a smart strategy that combines three-way liquid chromatography–mass spectrometry (LC–MS) data with second-order calibration method based on alternating trilinear decomposition (ATLD) algorithm was developed for simultaneous determination of ten β-blockers in human urine and plasma samples. This flexible strategy proved to be a useful tool to solve the problems of overlapped peaks and uncalibrated interferences encountered in quantitative LC–MS, and made the multi-targeted interference-free qualitative and quantitative analysis of β-blockers in complex matrices possible. The limits of detection were in the range of 2.0×10−5–6.2×10−3μgmL−1, and the average recoveries were between 90 and 110% with standard deviations and average relative prediction errors less than 10%, indicating that the strategy could provide satisfactory prediction results for ten β-blockers in human urine and plasma samples only using liquid chromatography hyphenated single–quadrupole mass spectrometer in full scan mode. To further confirm the feasibility and reliability of the proposed method, the same batch samples were analyzed by multiple reaction monitoring (MRM) method. T-test demonstrated that there are no significant differences between the prediction results of the two methods. Considering the advantages of fast, low-cost, high sensitivity, and no need of complicated chromatographic and tandem mass spectrometric conditions optimization, the proposed strategy is expected to be extended as an attractive alternative method to quantify analyte(s) of interest in complex systems such as cells, biological fluids, food, environment, pharmaceuticals and other complex samples.
    Keywords algorithms ; analytical chemistry ; beta-adrenergic antagonists ; cardiovascular diseases ; detection limit ; humans ; liquid chromatography ; mass spectrometry ; monitoring ; prediction ; quantitative analysis ; spectrometers ; t-test ; urine
    Language English
    Dates of publication 2014-1027
    Size p. 10-24.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2014.08.052
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  8. Article ; Online: S100A4 over-expression underlies lymph node metastasis and poor prognosis in colorectal cancer

    Li-Yong Huang, Ye Xu, Guo-Xiang Cai, Zu-Qing Guan, Wei-Qi Sheng, Hong-Fen Lu, Li-Qi Xie, Hao-Jie Lu, San-Jun Cai

    World Journal of Gastroenterology, Vol 17, Iss 1, Pp 69-

    2011  Volume 78

    Abstract: AIM: To develop lymph node metastasis (LNM)-associated biomarkers for colorectal cancer (CRC) using quantitative proteome analysis.METHODS: Differences in protein expression between primary CRC with LNM (LNM CRC) and without LNM (non-LNM CRC) were ... ...

    Abstract AIM: To develop lymph node metastasis (LNM)-associated biomarkers for colorectal cancer (CRC) using quantitative proteome analysis.METHODS: Differences in protein expression between primary CRC with LNM (LNM CRC) and without LNM (non-LNM CRC) were assessed using methyl esterification stable isotope labeling coupled with 2D liquid chromatography followed by tandem mass spectrometry (2D-LC-MS/MS). The relationship to clinicopathological parameters and prognosis of candidate biomarkers was examined using an independent sample set.RESULTS: Forty-three proteins were found to be differentially expressed by at least 2.5-fold in two types of CRC. S100A4 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by Western blotting, immunohistochemistry and real-time quantitative polymerase chain reaction. Further immunohistochemistry on another 112 CRC cases showed that overexpression of S100A4 frequently existed in LNM CRC compared with non-LNM CRC (P < 0.001). Overexpression of S100A4 was significantly associated with LNM (P < 0.001), advanced TNM stage (P < 0.001), increased 5-year recurrence rate (P < 0.001) and decreased 5-year overall survival rate (P < 0.001). Univariate and multivariate analyses indicated that S100A4 expression was an independent prognostic factor for recurrence and survival of CRC patients (P < 0.05).CONCLUSION: S100A4 might serve as a powerful biomarker for LNM and a prognostic factor in CRC.
    Keywords Colorectal cancer ; Lymph node metastasis ; Prognosis ; Proteome analysis ; S100A4 ; Diseases of the digestive system. Gastroenterology ; RC799-869 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Gastroenterology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Subject code 610
    Language English
    Publishing date 2011-01-01T00:00:00Z
    Publisher Baishideng Publishing Group Co. Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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