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  1. Article ; Online: Towards the direct detection of viral materials at the surface of protective face masks via infrared spectroscopy

    Vanessa Schorer / Julian Haas / Robert Stach / Vjekoslav Kokoric / Rüdiger Groß / Jan Muench / Tim Hummel / Harald Sobek / Jan Mennig / Boris Mizaikoff

    Scientific Reports, Vol 12, Iss 1, Pp 1-

    2022  Volume 9

    Abstract: Abstract The ongoing COVID-19 pandemic represents a considerable risk for the general public and especially for health care workers. To avoid an overloading of the health care system and to control transmission chains, the development of rapid and cost- ... ...

    Abstract Abstract The ongoing COVID-19 pandemic represents a considerable risk for the general public and especially for health care workers. To avoid an overloading of the health care system and to control transmission chains, the development of rapid and cost-effective techniques allowing for the reliable diagnosis of individuals with acute respiratory infections are crucial. Uniquely, the present study focuses on the development of a direct face mask sampling approach, as worn (i.e., used) disposable face masks contain exogenous environmental constituents, as well as endogenously exhaled breath aerosols. Optical techniques—and specifically infrared (IR) molecular spectroscopic techniques—are promising tools for direct virus detection at the surface of such masks. In the present study, a rapid and non-destructive approach for monitoring exposure scenarios via medical face masks using attenuated total reflection infrared spectroscopy is presented. Complementarily, IR external reflection spectroscopy was evaluated in comparison for rapid mask analysis. The utility of a face mask-based sampling approach was demonstrated by differentiating water, proteins, and virus-like particles sampled onto the mask. Data analysis using multivariate statistical algorithms enabled unambiguously classifying spectral signatures of individual components and biospecies. This approach has the potential to be extended towards the rapid detection of SARS-CoV-2—as shown herein for the example of virus-like particles which are morphologically equivalent to authentic virus—without any additional sample preparation or elaborate testing equipment at laboratory facilities. Therefore, this strategy may be implemented as a routine large-scale monitoring routine, e.g., at health care institutions, nursing homes, etc. ensuring the health and safety of medical personnel.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Advances in imprinting strategies for selective virus recognition a review

    Gast, Manuela / Harald Sobek / Boris Mizaikoff

    Trends in analytical chemistry. 2019 May, v. 114

    2019  

    Abstract: Molecular imprinting enables the fabrication of versatile functional polymers with pre-designed molecular target selectivity, inherent robustness, reusability, and reproducible production. Using advanced synthesis strategies, molecularly imprinted ... ...

    Abstract Molecular imprinting enables the fabrication of versatile functional polymers with pre-designed molecular target selectivity, inherent robustness, reusability, and reproducible production. Using advanced synthesis strategies, molecularly imprinted polymers even offer potential for virus recognition, which is of substantial interest as viral analysis and selective detection is a field of continuous development given increasing occurrence of viral variants and drug resistance. In this review, we discuss the most relevant virus imprinting strategies along with critical barriers for synthesizing virus-imprinted materials. Furthermore, selected applications are highlighted.
    Keywords drug resistance ; molecular imprinting ; polymers ; viruses
    Language English
    Dates of publication 2019-05
    Size p. 218-232.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2014041-1
    ISSN 0165-9936
    ISSN 0165-9936
    DOI 10.1016/j.trac.2019.03.010
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Enhanced Selectivity by Passivation: Molecular Imprints for Viruses with Exceptional Binding Properties

    Gast, Manuela / Boris Mizaikoff / Harald Sobek / Paul Walther / Stefanie Kühner

    Analytical chemistry. 2018 Mar. 05, v. 90, no. 9

    2018  

    Abstract: Inspired by the recognition processes found in biology such as enzyme–substrate and antibody–antigen interactions, synthetic systems with comparable molecular recognition properties have been investigated during recent years based on molecular imprinting ...

    Abstract Inspired by the recognition processes found in biology such as enzyme–substrate and antibody–antigen interactions, synthetic systems with comparable molecular recognition properties have been investigated during recent years based on molecular imprinting strategies. While materials with recognition capabilities for small molecules (i.e., with low molecular weight) have achieved substantial advancements, the synthesis of molecularly imprinted materials with virus recognition properties remains challenging to date. Likewise, protein–surface and protein–protein interactions are essential for a wide variety of biological applications in biotechnology. In biological sensor technology the coating of surfaces to prevent nonspecific adsorption interactions plays an important role. Particularly, polyethylene glycol (PEG) stands out for its high performance in preventing proteins from nonspecifically interactions. However, blocking agents such as the protein bovine serum albumin (BSA) can also be useful as unspecific binding prevention agents for passivation, without modification of the surface. Herein the influence of blocking agents as unspecific reaction components is investigated on the enhancements of selectivity from adenovirus-imprinted particles, whereas adenovirus was used as target species in molecular imprinting. Furthermore, quantitative polymerase chain reaction (qPCR) was used for the first time as virus quantification approach in this context.
    Keywords Adenoviridae ; adsorption ; antigen-antibody reactions ; binding properties ; biotechnology ; bovine serum albumin ; coatings ; enzyme substrates ; molecular imprinting ; molecular weight ; polyethylene glycol ; protein-protein interactions ; quantitative polymerase chain reaction ; viruses
    Language English
    Dates of publication 2018-0305
    Size p. 5576-5585.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.7b05148
    Database NAL-Catalogue (AGRICOLA)

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