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  1. Book ; Online ; Thesis: Untersuchungen zur Interaktion des Proliferating Cell Nuclear Antigen (PCNA) mit p15 und Identifizierung eines potenten peptidischen Inhibitors

    Hardebeck, Sarah Verfasser] / [Jose, Joachim [Akademischer Betreuer]

    2024  

    Author's details Sarah Hardebeck ; Betreuer: Joachim Jose
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universitäts- und Landesbibliothek Münster
    Publishing place Münster
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  2. Article ; Online: Identification of a potent PCNA-p15-interaction inhibitor by autodisplay-based peptide library screening.

    Hardebeck, Sarah / Jácobo Goebbels, Natalie / Michalski, Caroline / Schreiber, Sebastian / Jose, Joachim

    Microbial biotechnology

    2024  Volume 17, Issue 4, Page(s) e14471

    Abstract: Proliferating cell nuclear antigen (PCNA) is an essential factor for DNA metabolism. The influence of PCNA on DNA replication and repair, combined with the high expression rate of PCNA in various tumours renders PCNA a promising target for cancer therapy. ...

    Abstract Proliferating cell nuclear antigen (PCNA) is an essential factor for DNA metabolism. The influence of PCNA on DNA replication and repair, combined with the high expression rate of PCNA in various tumours renders PCNA a promising target for cancer therapy. In this context, an autodisplay-based screening method was developed to identify peptidic PCNA interaction inhibitors. A 12-mer randomized peptide library consisting of 2.54 × 10
    MeSH term(s) Proliferating Cell Nuclear Antigen/metabolism ; Proliferating Cell Nuclear Antigen/genetics ; Proliferating Cell Nuclear Antigen/chemistry ; Peptide Library ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Protein Binding ; Flow Cytometry ; Drug Evaluation, Preclinical/methods ; Cell Surface Display Techniques/methods ; Humans ; Peptides/metabolism ; Peptides/genetics ; Peptides/chemistry ; Peptides/pharmacology
    Chemical Substances Proliferating Cell Nuclear Antigen ; Peptide Library ; PCNA protein, human ; Peptides
    Language English
    Publishing date 2024-04-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2406063-X
    ISSN 1751-7915 ; 1751-7915
    ISSN (online) 1751-7915
    ISSN 1751-7915
    DOI 10.1111/1751-7915.14471
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A FRET-Based Assay for the Identification of PCNA Inhibitors.

    Hardebeck, Sarah / Schreiber, Sebastian / Adick, Annika / Langer, Klaus / Jose, Joachim

    International journal of molecular sciences

    2023  Volume 24, Issue 14

    Abstract: Proliferating cell nuclear antigen (PCNA) is the key regulator of human DNA metabolism. One important interaction partner is p15, involved in DNA replication and repair. Targeting the PCNA-p15 interaction is a promising therapeutic strategy against ... ...

    Abstract Proliferating cell nuclear antigen (PCNA) is the key regulator of human DNA metabolism. One important interaction partner is p15, involved in DNA replication and repair. Targeting the PCNA-p15 interaction is a promising therapeutic strategy against cancer. Here, a Förster resonance energy transfer (FRET)-based assay for the analysis of the PCNA-p15 interaction was developed. Next to the application as screening tool for the identification and characterization of PCNA-p15 interaction inhibitors, the assay is also suitable for the investigation of mutation-induced changes in their affinity. This is particularly useful for analyzing disease associated PCNA or p15 variants at the molecular level. Recently, the PCNA variant C148S has been associated with Ataxia-telangiectasia-like disorder type 2 (ATLD2). ATLD2 is a neurodegenerative disease based on defects in DNA repair due to an impaired PCNA. Incubation time dependent FRET measurements indicated no effect on PCNA
    MeSH term(s) Humans ; Proliferating Cell Nuclear Antigen/genetics ; Proliferating Cell Nuclear Antigen/metabolism ; Fluorescence Resonance Energy Transfer ; Neurodegenerative Diseases ; Protein Binding ; DNA Replication
    Chemical Substances Proliferating Cell Nuclear Antigen
    Language English
    Publishing date 2023-07-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241411858
    Database MEDical Literature Analysis and Retrieval System OnLINE

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