LIVIVO - Search results -

Search results

Result 1 - 10 of total 14

Search options

Article ; Online: Mycena species can be opportunist-generalist plant root invaders.

Harder, Christoffer Bugge / Hesling, Emily / Botnen, Synnøve S / Lorberau, Kelsey E / Dima, Bálint / von Bonsdorff-Salminen, Tea / Niskanen, Tuula / Jarvis, Susan G / Ouimette, Andrew / Hester, Alison / Hobbie, Erik A / Taylor, Andy F S / Kauserud, Håvard

Environmental microbiology

2023 Volume 25, Issue 10, Page(s) 1875–1893

Abstract: Traditional strict separation of fungi into ecological niches as mutualist, parasite or saprotroph is increasingly called into question. Sequences of assumed saprotrophs have been amplified from plant root interiors, and several saprotrophic genera can ... ...

Abstract	Traditional strict separation of fungi into ecological niches as mutualist, parasite or saprotroph is increasingly called into question. Sequences of assumed saprotrophs have been amplified from plant root interiors, and several saprotrophic genera can invade and interact with host plants in laboratory growth experiments. However, it is uncertain if root invasion by saprotrophic fungi is a widespread phenomenon and if laboratory interactions mirror field conditions. Here, we focused on the widespread and speciose saprotrophic genus Mycena and performed (1) a systematic survey of their occurrences (in ITS1/ITS2 datasets) in mycorrhizal roots of 10 plant species, and (2) an analysis of natural abundances of
MeSH term(s)	Mycorrhizae ; Agaricales ; Symbiosis ; Plants/microbiology ; Plant Roots/microbiology
Language	English
Publishing date	2023-05-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.16398
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- Full text online
- Order with fees

Article ; Online: In vitro evidence of root colonization suggests ecological versatility in the genus Mycena.

Thoen, Ella / Harder, Christoffer Bugge / Kauserud, Håvard / Botnen, Synnøve S / Vik, Unni / Taylor, Andy F S / Menkis, Audrius / Skrede, Inger

The New phytologist

2020 Volume 227, Issue 2, Page(s) 601–612

Abstract: The root-associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus ... ...

Abstract	The root-associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus Mycena are frequently major components in molecular studies of root-associated fungal communities, suggesting that an evaluation of their trophic status is warranted. Here, we report on interactions between a range of Mycena species and the plant Betula pendula. In all, 17 Mycena species were inoculated onto B. pendula seedlings. Physical interactions between hyphae and fine roots were examined using differential staining and fluorescence microscopy. Physiological interactions were investigated using
MeSH term(s)	Agaricales ; Mycorrhizae ; Plant Roots ; Seedlings ; Symbiosis
Language	English
Publishing date	2020-04-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.16545
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 868: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Ultrastructure and Phylogenetic Position of Regin rotiferus and Otto terricolus Genera et Species Novae (Bicosoecida, Heterokonta/Stramenopiles)

Harder, Christoffer Bugge / Flemming Ekelund / Sergey A. Karpov

Protist. 2014 Mar., v. 165

2014

Abstract: We describe two novel flagellates isolated from soil, Regin rotiferus and Otto terricolus, genera et species novae, which we cultivated and characterized by light and transmission electron microscopy and by 18S rDNA sequence analysis. Both strains ... ...

Abstract	We describe two novel flagellates isolated from soil, Regin rotiferus and Otto terricolus, genera et species novae, which we cultivated and characterized by light and transmission electron microscopy and by 18S rDNA sequence analysis. Both strains exhibit the key characteristic structural feature of Bicosoecida; i.e. the L-shaped cytostomal root system with an x-fiber, used for feeding. Otto terricolus displays unique novel morphological traits; thus, it has a basal swelling on each flagellum, a root 3/root 2 distribution of 10 + 1 microtubules, and an amoeboid stage in its life cycle. Regin rotiferus has flagella without swellings and a root 3/root 2 distribution of 7 + 3 microtubules, a pattern commonly observed in the Bicosoecida. We present an updated exhaustive maximum likelihood phylogeny of 48 cultured, complete or nearly complete (+1600bp) 18S rDNA Bicosoecida sequences. Both new species fall into a well-supported freshwater Siluaniidae clade, without being particularly closely related. The morphology and phylogeny do not conclusively support Rictus as a member of Bicosoecida.
Keywords	developmental stages ; flagellum ; freshwater ; microtubules ; new species ; nucleotide sequences ; phylogeny ; ribosomal DNA ; root systems ; sequence analysis ; soil ; transmission electron microscopy ; ultrastructure
Language	English
Dates of publication	2014-03
Size	p. 144-160.
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	2036014-9
ISSN	1618-0941 ; 1434-4610
ISSN (online)	1618-0941
ISSN	1434-4610
DOI	10.1016/j.protis.2014.01.004
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Ultrastructure and phylogenetic position of Regin rotiferus and Otto terricolus genera et species novae (Bicosoecida, Heterokonta/Stramenopiles).

Harder, Christoffer Bugge / Ekelund, Flemming / Karpov, Sergey A

Protist

2014 Volume 165, Issue 2, Page(s) 144–160

Abstract	We describe two novel flagellates isolated from soil, Regin rotiferus and Otto terricolus, genera et species novae, which we cultivated and characterized by light and transmission electron microscopy and by 18S rDNA sequence analysis. Both strains exhibit the key characteristic structural feature of Bicosoecida; i.e. the L-shaped cytostomal root system with an x-fiber, used for feeding. Otto terricolus displays unique novel morphological traits; thus, it has a basal swelling on each flagellum, a root 3/root 2 distribution of 10 + 1 microtubules, and an amoeboid stage in its life cycle. Regin rotiferus has flagella without swellings and a root 3/root 2 distribution of 7 + 3 microtubules, a pattern commonly observed in the Bicosoecida. We present an updated exhaustive maximum likelihood phylogeny of 48 cultured, complete or nearly complete (+1600 bp) 18S rDNA Bicosoecida sequences. Both new species fall into a well-supported freshwater Siluaniidae clade, without being particularly closely related. The morphology and phylogeny do not conclusively support Rictus as a member of Bicosoecida.
MeSH term(s)	Cluster Analysis ; DNA, Algal/chemistry ; DNA, Algal/genetics ; DNA, Ribosomal/chemistry ; DNA, Ribosomal/genetics ; DNA, Ribosomal Spacer/genetics ; Microscopy ; Organelles/ultrastructure ; Phylogeny ; RNA, Ribosomal, 18S/genetics ; Sequence Analysis, DNA ; Soil/parasitology ; Stramenopiles/classification ; Stramenopiles/genetics ; Stramenopiles/ultrastructure
Chemical Substances	DNA, Algal ; DNA, Ribosomal ; DNA, Ribosomal Spacer ; RNA, Ribosomal, 18S ; Soil
Language	English
Publishing date	2014-03
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2036014-9
ISSN	1618-0941 ; 1434-4610
ISSN (online)	1618-0941
ISSN	1434-4610
DOI	10.1016/j.protis.2014.01.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Aminobacter MSH1-Mineralisation of BAM in Sand-Filters Depends on Biological Diversity.

Ekelund, Flemming / Harder, Christoffer Bugge / Knudsen, Berith Elkær / Aamand, Jens

PloS one

2015 Volume 10, Issue 6, Page(s) e0128838

Abstract: BAM (2,6-dichlorobenzamide) is a metabolite of the pesticide dichlobenil. Naturally occurring bacteria that can utilize BAM are rare. Often the compound cannot be degraded before it reaches the groundwater and therefore it poses a serious threat to ... ...

Abstract	BAM (2,6-dichlorobenzamide) is a metabolite of the pesticide dichlobenil. Naturally occurring bacteria that can utilize BAM are rare. Often the compound cannot be degraded before it reaches the groundwater and therefore it poses a serious threat to drinking water supplies. The bacterial strain Aminobacter MSH1 is a BAM degrader and therefore a potential candidate to be amended to sand filters in waterworks to remediate BAM polluted drinking water. A common problem in bioremediation is that bacteria artificially introduced into new diverse environments often thrive poorly, which is even more unfortunate because biologically diverse environments may ensure a more complete decomposition. To test the bioaugmentative potential of MSH1, we used a serial dilution approach to construct microcosms with different biological diversity. Subsequently, we amended Aminobacter MSH1 to the microcosms in two final concentrations; i.e. 10(5) cells mL(-1) and 10(7) cells mL(-1). We anticipated that BAM degradation would be most efficient at "intermediate diversities" as low diversity would counteract decomposition because of incomplete decomposition of metabolites and high diversity would be detrimental because of eradication of Aminobacter MSH1. This hypothesis was only confirmed when Aminobacter MSH1 was amended in concentrations of 10(5) cells mL(-1). Our findings suggest that Aminobacter MSH1 is a very promising bioremediator at several diversity levels.
MeSH term(s)	Bacterial Load ; Benzamides/metabolism ; Construction Materials/microbiology ; Phyllobacteriaceae/metabolism ; Soil Microbiology
Chemical Substances	Benzamides ; 2,6-dichlorobenzamide (E9JWF529EB)
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0128838
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Full text

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Local diversity of heathland Cercozoa explored by in-depth sequencing.

Harder, Christoffer Bugge / Rønn, Regin / Brejnrod, Asker / Bass, David / Al-Soud, Waleed Abu / Ekelund, Flemming

The ISME journal

2016 Volume 10, Issue 10, Page(s) 2488–2497

Abstract: Cercozoa are abundant free-living soil protozoa and quantitatively important in soil food webs; yet, targeted high-throughput sequencing (HTS) has not yet been applied to this group. Here we describe the development of a targeted assay to explore ... ...

Abstract	Cercozoa are abundant free-living soil protozoa and quantitatively important in soil food webs; yet, targeted high-throughput sequencing (HTS) has not yet been applied to this group. Here we describe the development of a targeted assay to explore Cercozoa using HTS, and we apply this assay to measure Cercozoan community response to drought in a Danish climate manipulation experiment (two sites exposed to artificial drought, two unexposed). Based on a comparison of the hypervariable regions of the 18S ribosomal DNA of 193 named Cercozoa, we concluded that the V4 region is the most suitable for group-specific diversity analysis. We then designed a set of highly specific primers (encompassing ~270 bp) for 454 sequencing. The primers captured all major cercozoan groups; and >95% of the obtained sequences were from Cercozoa. From 443 350 high-quality short reads (>300 bp), we recovered 1585 operational taxonomic units defined by >95% V4 sequence similarity. Taxonomic annotation by phylogeny enabled us to assign >95% of our reads to order level and ~85% to genus level despite the presence of a large, hitherto unknown diversity. Over 40% of the annotated sequences were assigned to Glissomonad genera, whereas the most common individually named genus was the euglyphid Trinema. Cercozoan diversity was largely resilient to drought, although we observed a community composition shift towards fewer testate amoebae.
MeSH term(s)	Biodiversity ; Cercozoa/classification ; Cercozoa/genetics ; Cercozoa/isolation & purification ; DNA Primers/genetics ; DNA, Ribosomal/genetics ; High-Throughput Nucleotide Sequencing ; Phylogeny ; Soil/parasitology
Chemical Substances	DNA Primers ; DNA, Ribosomal ; Soil
Language	English
Publishing date	2016
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2406536-5
ISSN	1751-7370 ; 1751-7362
ISSN (online)	1751-7370
ISSN	1751-7362
DOI	10.1038/ismej.2016.31
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 7773: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Towards diagnostic metagenomics of Campylobacter in fecal samples.

Andersen, Sandra Christine / Kiil, Kristoffer / Harder, Christoffer Bugge / Josefsen, Mathilde Hasseldam / Persson, Søren / Nielsen, Eva Møller / Hoorfar, Jeffrey

BMC microbiology

2017 Volume 17, Issue 1, Page(s) 133

Abstract: Background: The development of diagnostic metagenomics is driven by the need for universal, culture-independent methods for detection and characterization of pathogens to substitute the time-consuming, organism-specific, and often culture-based ... ...

Abstract	Background: The development of diagnostic metagenomics is driven by the need for universal, culture-independent methods for detection and characterization of pathogens to substitute the time-consuming, organism-specific, and often culture-based laboratory procedures for epidemiological source-tracing. Some of the challenges in diagnostic metagenomics are, that it requires a great next-generation sequencing depth and unautomated data analysis. Results: DNA from human fecal samples spiked with 7.75 × 10 Conclusions: Sensitivity in diagnostic metagenomics is improving and has reached a clinically relevant level. There are still challenges to overcome before real-time diagnostic metagenomics can replace quantitative polymerase chain reaction (qPCR) or culture-based surveillance and diagnostics, but it is a promising new technology.
Language	English
Publishing date	2017-06-08
Publishing country	England
Document type	Journal Article
ISSN	1471-2180
ISSN (online)	1471-2180
DOI	10.1186/s12866-017-1041-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: The bacterial microbiota in first-void urine from men with and without idiopathic urethritis.

Frølund, Maria / Wikström, Arne / Lidbrink, Peter / Abu Al-Soud, Waleed / Larsen, Niels / Harder, Christoffer Bugge / Sørensen, Søren Johannes / Jensen, Jørgen Skov / Ahrens, Peter

PloS one

2018 Volume 13, Issue 7, Page(s) e0201380

Abstract: Background: Non-gonococcal urethritis (NGU) is a common syndrome in men. NGU may have several causes, but many cases are caused by sexually transmitted infections that may also cause complications in their female partners. Chlamydia trachomatis and ... ...

Abstract	Background: Non-gonococcal urethritis (NGU) is a common syndrome in men. NGU may have several causes, but many cases are caused by sexually transmitted infections that may also cause complications in their female partners. Chlamydia trachomatis and Mycoplasma genitalium are the most common causes of NGU, but in up to 35% of the cases, none of the known viral or bacterial causes are found. Traditionally, pathogens have been detected using various culture techniques that may not identify all species present in the urethra. To address this, we used culture-independent methods for analysis of the male urethral microbiota. Methods: This case-control study analysed first void urine samples, collected at STD clinics in Stockholm, Sweden from men with idiopathic urethritis (IU), i.e. negative for Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Ureaplasma urealyticum, Trichomonas vaginalis, adenovirus, and herpes simplex virus type 1 and -2 together with samples from men without urethritis. Forty-six controls and 39 idiopathic urethritis patients were analysed. Results: The microbiota was highly diverse: None of the 302 operational taxonomic units (OTUs) found in negative controls and IU patients were found in all of the samples or even in all of the samples in one group. More than 50% of the OTUs were only found in one or two of the total of 85 samples. Still the most dominant 1/6 of the genera constituted 79% of the sequences. Hierarchical clustering in a heatmap showed no specific clustering of patients or controls. A number of IU patient samples were dominated by a single genus previously related to urethritis (Gardnerella, Haemophilus, Ureaplasma). Conclusion: The male urethra contain a very diverse composition of bacteria, even in healthy controls. NGU may be caused by a number of different bacteria but more studies including a higher number of samples are needed for elucidation of the role of each species.
MeSH term(s)	Adenoviridae/classification ; Adenoviridae/genetics ; Adult ; Gram-Negative Bacteria/classification ; Gram-Negative Bacteria/genetics ; Herpesvirus 1, Human/genetics ; Herpesvirus 2, Human/genetics ; Humans ; Male ; Middle Aged ; Urethra/microbiology ; Urethra/virology ; Urethritis/microbiology ; Urethritis/urine ; Urethritis/virology ; Urine/microbiology ; Urine/virology
Language	English
Publishing date	2018-07-27
Publishing country	United States
Document type	Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0201380
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: A comparison between ITS phylogenetic relationships and morphological species recognition within Mycena sect. Calodontes in Northern Europe

Harder, Christoffer Bugge / Læssøe, Thomas / Kjøller, Rasmus / Frøslev, Tobias G

Mycological progress. 2010 Aug., v. 9, no. 3

2010

Abstract: The members of Mycena sect. Calodontes (Tricholomataceae s.l., Basidiomycota) are characterised by a collybioid aspect and more or less purplish to reddish colours and a distinct raphanoid odour. In Europe, nine species have been recognised though some ... ...

Abstract	The members of Mycena sect. Calodontes (Tricholomataceae s.l., Basidiomycota) are characterised by a collybioid aspect and more or less purplish to reddish colours and a distinct raphanoid odour. In Europe, nine species have been recognised though some of these based on somewhat dubious morphological differences. Historically, most were assigned to Mycena pura. However, since Mycena pura displays one of the most striking colour variabilities within European agarics, many attempts have been made to subdivide it into independent entities, and several forms, varieties and species have been split from Mycena pura s.l. based largely on differences in colouration, gross macromorphology or other phenetic traits. We developed a large sample of ITS sequences of all species of sect. Calodontes known from Europe for which vouchers exist. Furthermore, partial LSU data were developed and additional sequences downloaded from GENBANK to assess the relationship of Calodontes with other Mycena spp. We show that most Calodontes form a monophyletic group including a few North and South American collections, but that this cannot be conclusively shown when an additional North American sequence is added. For all other species than M. pura and M. diosma, we found morphological species recognition to be in agreement with the ITS data. Several significantly different clades can be recognised within the M. pura morphospecies, none of which can be linked to the observed (and described by proxy) colour varieties/forms. Indications of a possible environmental basis of the colour differentiation in the M. pura morphospecies are discussed.
Keywords	Mycena ; Tricholomataceae ; color ; internal transcribed spacers ; odors ; phylogeny ; Northern European region
Language	English
Dates of publication	2010-08
Size	p. 395-405.
Publisher	Springer-Verlag
Publishing place	Berlin/Heidelberg
Document type	Article
ZDB-ID	2226747-5
ISSN	1861-8952 ; 1617-416X
ISSN (online)	1861-8952
ISSN	1617-416X
DOI	10.1007/s11557-009-0648-7
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article: Amyloidity is not diagnostic for species in the Mycena pearsoniana complex (Mycena sectio Calodontes)

Harder, Christoffer Bugge / Lodge, D. Jean / Petersen, Ronald H / Hughes, Karen W / Blanco, Joaquin Cifuentes / Frøslev, Tobias Guldberg / Læssøe, Thomas

Mycological progress. 2012 Aug., v. 11, no. 3

2012

Abstract: In Mycena sectio Calodontes with otherwise amyloid spores, the inamyloid spores of Mycena pearsoniana Dennis ex Singer were a distinguishing feature for this species and its subsection Violacella. Although the original concept of this species was ... ...

Abstract	In Mycena sectio Calodontes with otherwise amyloid spores, the inamyloid spores of Mycena pearsoniana Dennis ex Singer were a distinguishing feature for this species and its subsection Violacella. Although the original concept of this species was European, Singer chose to typify it with material collected in Mexico. The name has since been applied to all European collections with inamyloid spores and decurrent lamellae. Our phylogenetic analysis of 91 ITS sequences from European, North and South American Calodontes collections shows that European collections identified as M. pearsoniana fall into two well-supported sibling clades together with both inamyloid and weakly amyloid North American collections. Since the holotype of M. pearsoniana is in an advanced state of decay, we have selected an epitype from a North American locality with a climate comparable to the Mexican type locality. Our results show weakly and inamyloid spore reactions to be homoplastic in Calodontes, and furthermore that spores of M. pearsoniana can show either amyloid or inamyloid reactions interchangeably. This raises doubt about the taxonomic value of this trait in Mycena systematics.
Keywords	Mycena ; amyloid ; climate ; internal transcribed spacers ; phylogeny ; spores ; Mexico
Language	English
Dates of publication	2012-08
Size	p. 725-732.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	2226747-5
ISSN	1861-8952 ; 1617-416X
ISSN (online)	1861-8952
ISSN	1617-416X
DOI	10.1007/s11557-011-0782-x
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

To top

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito