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  1. Article ; Online: Developing a checklist for reporting research using simulated patient methodology (CRiSP): a consensus study.

    Amaratunge, Suvini / Harrison, Morgan / Clifford, Rhonda / Seubert, Liza / Page, Amy / Bond, Christine

    The International journal of pharmacy practice

    2021  Volume 29, Issue 3, Page(s) 218–227

    Abstract: Objectives: Simulated patients are increasingly used to measure outcomes in health services but reporting is suboptimal. This study aims to create a checklist for the reporting of simulated patient (SP) methodology.: Methods: This was a Delphi study. ...

    Abstract Objectives: Simulated patients are increasingly used to measure outcomes in health services but reporting is suboptimal. This study aims to create a checklist for the reporting of simulated patient (SP) methodology.
    Methods: This was a Delphi study. The authors of health service research studies using SP methodology were invited to participate. Round 1 questionnaire assessed the applicability of the TIDieR (Template for Intervention Description and Replication) reporting checklist for SP methodology and asked for rewording of/additional items. Responses were thematically analysed to generate Round 2 items in which participants rated each item for importance (seven-point Likert scale) and median, mode and IQR were calculated. In Round 3, participants were invited to rescore their Round 2 responses. Consensus was defined as an IQR ≤ 1 (Extremely important) and median ≤ 2 (Very important). All consensus items were considered for inclusion in the checklist. Similarly, worded items were rationalised and items not specific to SP methodology or other existing checklists were excluded.
    Key findings: Twenty-nine authors participated in Round 1 and a further seven for Rounds 2 and 3. Twenty-six responses were analysed for Round 1, 30 for Round 2 and 28 for Round 3. There was consensus on 29 of 54 items in Round 2 and 45 of 63 items in Round 3. The final checklist comprised 28 items.
    Conclusions: A new reporting checklist to guide the reporting of studies, using simulated patients, complementary to CONSORT or STROBE, has been developed and will now be tested for usability.
    MeSH term(s) Checklist ; Consensus ; Delphi Technique ; Humans ; Research Design ; Surveys and Questionnaires
    Language English
    Publishing date 2021-04-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 1087040-4
    ISSN 2042-7174 ; 0961-7671
    ISSN (online) 2042-7174
    ISSN 0961-7671
    DOI 10.1093/ijpp/riaa002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Cellular mechanotransduction of human osteoblasts in microgravity.

    Wubshet, Nadab H / Cai, Grace / Chen, Samuel J / Sullivan, Molly / Reeves, Mark / Mays, David / Harrison, Morgan / Varnado, Paul / Yang, Benjamin / Arreguin-Martinez, Esmeralda / Qu, Yunjia / Lin, Shan-Shan / Duran, Pamela / Aguilar, Carlos / Giza, Shelby / Clements, Twyman / Liu, Allen P

    NPJ microgravity

    2024  Volume 10, Issue 1, Page(s) 35

    Abstract: Astronauts experience significant and rapid bone loss as a result of an extended stay in space, making the International Space Station (ISS) the perfect laboratory for studying osteoporosis due to the accelerated nature of bone loss on the ISS. This ... ...

    Abstract Astronauts experience significant and rapid bone loss as a result of an extended stay in space, making the International Space Station (ISS) the perfect laboratory for studying osteoporosis due to the accelerated nature of bone loss on the ISS. This prompts the question, how does the lack of load due to zero-gravity propagate to bone-forming cells, human fetal osteoblasts (hFOBs), altering their maturation to mineralization? Here, we aim to study the mechanotransduction mechanisms by which bone loss occurs in microgravity. Two automated experiments, microfluidic chips capable of measuring single-cell mechanics via aspiration and cell spheroids incubated in pressure-controlled chambers, were each integrated into a CubeLab deployed to the ISS National Laboratory. For the first experiment, we report protrusion measurements of aspirated cells after exposure to microgravity at the ISS and compare these results to ground control conducted inside the CubeLab. We found slightly elongated protrusions for space samples compared to ground samples indicating softening of hFOB cells in microgravity. In the second experiment, we encapsulated osteoblast spheroids in collagen gel and incubated the samples in pressure-controlled chambers. We found that microgravity significantly reduced filamentous actin levels in the hFOB spheroids. When subjected to pressure, the spheroids exhibited increased pSMAD1/5/9 expression, regardless of the microgravity condition. Moreover, microgravity reduced YAP expression, while pressure increased YAP levels, thus restoring YAP expression for spheroids in microgravity. Our study provides insights into the influence of microgravity on the mechanical properties of bone cells and the impact of compressive pressure on cell signaling in space.
    Language English
    Publishing date 2024-03-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2823626-9
    ISSN 2373-8065
    ISSN 2373-8065
    DOI 10.1038/s41526-024-00386-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Cellular mechanotransduction of human osteoblasts in microgravity.

    Wubshet, Nadab H / Cai, Grace / Chen, Samuel J / Sullivan, Molly / Reeves, Mark / Mays, David / Harrison, Morgan / Varnado, Paul / Yang, Benjamin / Arreguin-Martinez, Esmeralda / Qu, Yunjia / Lin, Shan-Shan / Duran, Pamela / Aguilar, Carlos / Giza, Shelby / Clements, Twyman / Liu, Allen P

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Astronauts experience significant and rapid bone loss as a result of an extended stay in space, making the International Space Station (ISS) the perfect laboratory for studying osteoporosis due to the accelerated nature of bone loss on the ISS. This ... ...

    Abstract Astronauts experience significant and rapid bone loss as a result of an extended stay in space, making the International Space Station (ISS) the perfect laboratory for studying osteoporosis due to the accelerated nature of bone loss on the ISS. This prompts the question, how does the lack of load due to zero-gravity propagate to bone-forming cells, human fetal osteoblasts (hFOBs), altering their maturation to mineralization? Here, we aim to study the mechanotransduction mechanisms by which bone loss occurs in microgravity. Two automated experiments, 4 microfluidic chips capable of measuring single-cell mechanics of hFOBs via aspiration and cell spheroids incubated in pressure-controlled chambers, were each integrated into a CubeLab deployed to the ISS National Laboratory. For the first experiment, we report protrusion measurements of aspirated cells after exposure to microgravity at the ISS and compare these results to ground control conducted inside the CubeLab. Our analysis revealed slightly elongated protrusions for space samples compared to ground samples indicating softening of hFOB cells in microgravity. In the second experiment, we encapsulated osteoblast spheroids in collagen gel and incubated the samples in pressure-controlled chambers. We found that microgravity significantly reduced filamentous actin levels in the hFOB spheroids. When subjected to pressure, the spheroids exhibited increased pSMAD1/5/9 expression, regardless of the microgravity condition. Moreover, microgravity reduced YAP expression, while pressure increased YAP levels, thus restoring YAP expression for spheroids in microgravity. Our study provides insights into the influence of microgravity on the mechanical properties of bone cells and the impact of compressive pressure on cell behavior and signaling in space.
    Language English
    Publishing date 2024-03-03
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.03.583164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Assessing the reporting quality of simulated patient studies in pharmacy research using a novel checklist (CRiSP).

    Amaratunge, Suvini / Harrison, Morgan / Perry, Danae / Bond, Christine / Ceulemans, Michael / Foulon, Veerle / Clifford, Rhonda / Seubert, Liza

    Research in social & administrative pharmacy : RSAP

    2021  Volume 18, Issue 2, Page(s) 2301–2307

    Abstract: Background: Use of simulated patients (SP) to assess the quality of pharmacy services and impact of interventions is increasing. The CRiSP (Checklist for Reporting research using Simulated Patient methodology) checklist was recently developed, assisting ...

    Abstract Background: Use of simulated patients (SP) to assess the quality of pharmacy services and impact of interventions is increasing. The CRiSP (Checklist for Reporting research using Simulated Patient methodology) checklist was recently developed, assisting researchers to report items necessary to meet a minimum agreed standard.
    Objective(s): To identify which CRiSP items were reported in SP studies for community pharmacy research, identify any gaps in reporting and describe the overall quality of reporting for the SP studies identified.
    Methods: Papers published during 2018-2020 using SP methodology in community pharmacy settings were identified from MEDLINE and Embase. The 50 most recent ones were selected. Data were extracted independently and in duplicate. Each paper received a coded numerical value denoting compliance with each item of CRiSP (1 = yes, 2 = no, 3 = unclear, 4 = not applicable, 5 = partially complete). Data were analysed using Microsoft Excel and reported as frequencies and percentages of each code for the checklist items, across the 50 papers.
    Results: No paper fulfilled all items in the CRiSP checklist. The mode(s) of delivery of SP assessments (item 17) was reported in all papers, while use of the term SP (item 1); number of SPs (4a); scenario details (9a); describing procedures
    Conclusions: This review identified that pharmacy research involving SP methodology is often inadequately reported by researchers. The CRiSP checklist is a comprehensive tool to assess the quality of SP methodology reporting but may require some refinement to ensure consistency in use.
    MeSH term(s) Checklist ; Humans ; Pharmaceutical Services ; Pharmacy Research ; Research Report
    Language English
    Publishing date 2021-04-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2192059-X
    ISSN 1934-8150 ; 1551-7411
    ISSN (online) 1934-8150
    ISSN 1551-7411
    DOI 10.1016/j.sapharm.2021.04.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Transcatheter Aortic Valve Replacement Patient Care Improvements; It Takes a Team.

    Keegan, Patricia A / Mitchell, Rae / Stoneman, Christine / Arrington, William Shane / Spahr, Angela / Brown, Thomas / Biven, Kelby / Donovan, Emily / Kalinke, Louisa / Mathew, Preethy / Harrison, Morgan / Jones, Emily / Higgins, Mary / Hester, Kenya / Gaston, Jeanette / Mortorano, Cecilia

    Critical care nursing clinics of North America

    2022  Volume 34, Issue 2, Page(s) 205–214

    Abstract: Team-based care has been recommended by numerous cardiovascular organizations involving the treatment of valvular heart disease. Utilization of the cardiovascular team (CVT) in valvular programs has been discussed but there is a paucity of data involving ...

    Abstract Team-based care has been recommended by numerous cardiovascular organizations involving the treatment of valvular heart disease. Utilization of the cardiovascular team (CVT) in valvular programs has been discussed but there is a paucity of data involving team roles, backgrounds, or expectations. This article will describe a single health system and the roles of the CVT involved in the transcatheter aortic valve replacement (TAVR) program.
    MeSH term(s) Aortic Valve Stenosis/surgery ; Humans ; Patient Care ; Patient Care Team ; Risk Factors ; Transcatheter Aortic Valve Replacement ; Treatment Outcome
    Language English
    Publishing date 2022-04-28
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1313997-6
    ISSN 1558-3481 ; 0899-5885
    ISSN (online) 1558-3481
    ISSN 0899-5885
    DOI 10.1016/j.cnc.2022.02.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Genome Sequences of Subcluster M2 Mycobacteriophages Estes and Aziz.

    Fitzgerald, Sara K / Johnson, Eleanor H / Storz, Sophie H R / Ballard, Claire / Battaglia, Samantha / Boice, Mikelle / Bramwell-Butcher, Joshua / Dedinsky, Megan / DeKlotz, Joshua / Diaz, Izabel / Engley, Andrew / Ernst, Lindsey / Gonzales, Erica / Groscost, Alyssa / Grosser, Paige / Haider, Alicia / Harrison, Morgan / Husler, Kurt / Lau, Jalisa /
    Monlux, Melina / Paratore, Jason / Ruesch, Trevor / Schlesinger, Mikaela / Scholes, Anthony / Poxleitner, Marianne K / Anders, Kirk R

    Microbiology resource announcements

    2021  Volume 10, Issue 10

    Abstract: Estes and Aziz are mycobacteriophages that were isolated ... ...

    Abstract Estes and Aziz are mycobacteriophages that were isolated on
    Language English
    Publishing date 2021-03-11
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00104-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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